Facklamia hominis CCUG 36813
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1717 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K1LRY1|K1LRY1_9LACT Tetracycline resistance protein tetM OS=Facklamia hominis CCUG 36813 OX=883111 GN=HMPREF9706_01072 PE=3 SV=1
MM1 pKa = 7.34 TNEE4 pKa = 4.33 EE5 pKa = 4.76 LEE7 pKa = 4.37 SMIQEE12 pKa = 3.49 IHH14 pKa = 6.48 KK15 pKa = 10.57 NIVEE19 pKa = 4.21 NEE21 pKa = 4.0 YY22 pKa = 11.19 SSDD25 pKa = 3.95 SIKK28 pKa = 11.22 DD29 pKa = 3.43 KK30 pKa = 11.05 LADD33 pKa = 3.69 YY34 pKa = 11.26 ADD36 pKa = 5.02 DD37 pKa = 5.55 DD38 pKa = 4.62 DD39 pKa = 6.33 AISSSSLVAFVLNEE53 pKa = 3.73 NRR55 pKa = 11.84 HH56 pKa = 4.59 YY57 pKa = 8.46 TCTMIYY63 pKa = 10.49 SLFSRR68 pKa = 11.84 LLDD71 pKa = 3.1 QGYY74 pKa = 9.48 FSDD77 pKa = 5.57 DD78 pKa = 3.49 NPP80 pKa = 3.72
Molecular weight: 9.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.781
IPC2_protein 3.986
IPC_protein 3.923
Toseland 3.732
ProMoST 4.088
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.859
Rodwell 3.757
Grimsley 3.643
Solomon 3.897
Lehninger 3.859
Nozaki 4.037
DTASelect 4.253
Thurlkill 3.783
EMBOSS 3.859
Sillero 4.05
Patrickios 1.926
IPC_peptide 3.897
IPC2_peptide 4.024
IPC2.peptide.svr19 3.934
Protein with the highest isoelectric point:
>tr|K1LR34|K1LR34_9LACT Uncharacterized protein OS=Facklamia hominis CCUG 36813 OX=883111 GN=HMPREF9706_00767 PE=4 SV=1
LL1 pKa = 7.37 NSISVTISTLFSTLNATRR19 pKa = 11.84 VTISTIFSTLKK30 pKa = 10.24 ALRR33 pKa = 11.84 VTISTLFSTLNSISVTISTLFSTLSAMRR61 pKa = 11.84 VTIPTRR67 pKa = 11.84 CSTLNSIRR75 pKa = 11.84 VTISTPFSTLSSISVTISTLFSTLNTARR103 pKa = 11.84 VTISTLFSTLSPTRR117 pKa = 11.84 ATIATLFGTLRR128 pKa = 11.84 TLRR131 pKa = 11.84 VTIPPHH137 pKa = 6.25 FSTLRR142 pKa = 11.84 TLRR145 pKa = 11.84 ITISTRR151 pKa = 11.84 FGTLSSLRR159 pKa = 11.84 VTIPTRR165 pKa = 11.84 FGTLRR170 pKa = 11.84 TLRR173 pKa = 11.84 VTIYY177 pKa = 10.39 TRR179 pKa = 11.84 FSTLSPTRR187 pKa = 11.84 VTIPTRR193 pKa = 11.84 CSTLSTLRR201 pKa = 11.84 VTISTHH207 pKa = 5.94 FSTLSSLRR215 pKa = 11.84 VTIYY219 pKa = 10.53 TRR221 pKa = 11.84 FSTLSSISVTISTRR235 pKa = 11.84 FSTLNTARR243 pKa = 11.84 VTISTLFSTLSPTRR257 pKa = 11.84 VTISTLFSTLSPISVTT273 pKa = 3.61
Molecular weight: 29.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.392
IPC2_protein 11.008
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.12
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.842
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.146
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1717
0
1717
552778
29
3044
321.9
36.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.218 ± 0.056
0.716 ± 0.017
5.977 ± 0.061
6.669 ± 0.078
4.231 ± 0.056
6.216 ± 0.058
2.062 ± 0.027
7.407 ± 0.07
6.248 ± 0.064
10.534 ± 0.09
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.555 ± 0.032
4.387 ± 0.044
3.552 ± 0.041
5.352 ± 0.069
4.176 ± 0.042
6.362 ± 0.053
4.924 ± 0.054
6.464 ± 0.044
0.918 ± 0.02
4.031 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here