Rhizobacter sp. Root1221

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiales genera incertae sedis; Rhizobacter; unclassified Rhizobacter

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5887 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q6WFD7|A0A0Q6WFD7_9BURK Uncharacterized protein OS=Rhizobacter sp. Root1221 OX=1736433 GN=ASC87_28750 PE=4 SV=1
MM1 pKa = 7.73TDD3 pKa = 4.08DD4 pKa = 4.23KK5 pKa = 11.62GATATQAVXLTIXGTNDD22 pKa = 2.91APVITSALQSGAVKK36 pKa = 10.27EE37 pKa = 4.23DD38 pKa = 3.24TALTVTGTVSSSDD51 pKa = 3.38VDD53 pKa = 3.81HH54 pKa = 6.96GATAAYY60 pKa = 9.89SGNTTGSYY68 pKa = 11.24GSFAVDD74 pKa = 3.29ASTGVWTYY82 pKa = 10.56TLDD85 pKa = 3.89NANHH89 pKa = 5.88QNLAQDD95 pKa = 3.72EE96 pKa = 4.85THH98 pKa = 6.67TEE100 pKa = 4.2TFTVTVTDD108 pKa = 4.13DD109 pKa = 3.12KK110 pKa = 11.54GATVTQDD117 pKa = 3.34VILTITGTNDD127 pKa = 3.04GPVITSAVQSGVVKK141 pKa = 10.58EE142 pKa = 4.26DD143 pKa = 3.25TALTATGTVASSDD156 pKa = 3.58VDD158 pKa = 3.64HH159 pKa = 7.0GATAAYY165 pKa = 9.89SGNTTGSYY173 pKa = 11.05GSFVVDD179 pKa = 4.07AGTGVWTYY187 pKa = 10.63TLDD190 pKa = 3.8NTNHH194 pKa = 5.73QNLAQDD200 pKa = 3.72EE201 pKa = 4.84THH203 pKa = 6.6TEE205 pKa = 4.12TFAVTVTDD213 pKa = 4.47DD214 pKa = 4.23FGATAVQNVLLTITGTNDD232 pKa = 2.59APAITSAVQSGTVKK246 pKa = 10.65EE247 pKa = 4.35DD248 pKa = 3.23TALTATGTVASSDD261 pKa = 3.58VDD263 pKa = 3.64HH264 pKa = 7.0GATAAYY270 pKa = 9.91SGNTTGTYY278 pKa = 10.79GSFEE282 pKa = 4.1VDD284 pKa = 2.54AGTGVWTYY292 pKa = 10.63TLDD295 pKa = 3.8NTNHH299 pKa = 5.61QNLAQGEE306 pKa = 4.61SQHH309 pKa = 5.43EE310 pKa = 4.5TFTVTVTDD318 pKa = 4.43DD319 pKa = 3.38KK320 pKa = 11.63GATATQDD327 pKa = 3.52VIVTITGTNDD337 pKa = 2.9VPVITSTAQSGTVKK351 pKa = 10.62EE352 pKa = 4.44DD353 pKa = 3.29TTLTATGTVASSDD366 pKa = 3.58VDD368 pKa = 3.64HH369 pKa = 7.0GATAAYY375 pKa = 9.91SGNTTGTYY383 pKa = 10.54GSFVVDD389 pKa = 4.06AGTGVWTYY397 pKa = 7.01TLRR400 pKa = 11.84DD401 pKa = 3.69SSRR404 pKa = 11.84GPDD407 pKa = 3.4DD408 pKa = 5.22HH409 pKa = 6.92GHH411 pKa = 4.58QQ412 pKa = 3.93

Molecular weight: 41.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q6X2F3|A0A0Q6X2F3_9BURK Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit C OS=Rhizobacter sp. Root1221 OX=1736433 GN=gatC PE=3 SV=1
MM1 pKa = 7.33KK2 pKa = 9.38RR3 pKa = 11.84TYY5 pKa = 9.73QASKK9 pKa = 7.81TRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.79GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5887

0

5887

1990138

30

5752

338.1

36.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.064 ± 0.038

0.849 ± 0.01

5.57 ± 0.025

4.959 ± 0.029

3.46 ± 0.021

8.506 ± 0.033

2.347 ± 0.016

3.854 ± 0.024

2.893 ± 0.031

10.619 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.274 ± 0.016

2.537 ± 0.023

5.442 ± 0.031

3.699 ± 0.018

7.23 ± 0.033

5.414 ± 0.03

5.573 ± 0.038

8.169 ± 0.028

1.481 ± 0.015

2.058 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski