Frankia sp. EUN1h

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Frankiales; Frankiaceae; Frankia; unclassified Frankia

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7402 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S1QLY2|A0A1S1QLY2_9ACTN Uncharacterized protein OS=Frankia sp. EUN1h OX=1834515 GN=BCD49_22455 PE=4 SV=1
MM1 pKa = 6.76STAEE5 pKa = 4.0IAQPPYY11 pKa = 9.98RR12 pKa = 11.84PSFAAIPTASAHH24 pKa = 6.23HH25 pKa = 6.76AATSPATGLVTAADD39 pKa = 3.9SDD41 pKa = 4.31APAGDD46 pKa = 3.65AVLGVSGIDD55 pKa = 3.42ASSDD59 pKa = 3.08ASGVSGVSAVMPAPAGSGLTHH80 pKa = 7.56PDD82 pKa = 3.25HH83 pKa = 7.69ADD85 pKa = 3.47GDD87 pKa = 4.44DD88 pKa = 3.3ACIADD93 pKa = 3.83GG94 pKa = 4.44

Molecular weight: 8.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S1QSJ9|A0A1S1QSJ9_9ACTN Uncharacterized protein OS=Frankia sp. EUN1h OX=1834515 GN=BCD49_18500 PE=4 SV=1
MM1 pKa = 7.55LRR3 pKa = 11.84LCNLPSTPGDD13 pKa = 3.45VRR15 pKa = 11.84ASRR18 pKa = 11.84HH19 pKa = 4.17PAARR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84SRR27 pKa = 11.84HH28 pKa = 5.2RR29 pKa = 11.84RR30 pKa = 11.84WHH32 pKa = 5.55VGSPVLVIGLAAGGGLAAGGGLAAGGGLAALLTATTLATRR72 pKa = 11.84RR73 pKa = 11.84RR74 pKa = 11.84RR75 pKa = 11.84RR76 pKa = 11.84VARR79 pKa = 11.84TTRR82 pKa = 11.84RR83 pKa = 11.84GAHH86 pKa = 5.06RR87 pKa = 11.84TT88 pKa = 3.5

Molecular weight: 9.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7402

0

7402

2529610

29

6661

341.7

36.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.603 ± 0.053

0.738 ± 0.007

6.247 ± 0.022

5.007 ± 0.023

2.726 ± 0.015

9.674 ± 0.03

2.117 ± 0.012

3.343 ± 0.019

1.496 ± 0.019

10.337 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.615 ± 0.011

1.641 ± 0.015

6.485 ± 0.031

2.542 ± 0.013

8.326 ± 0.027

4.989 ± 0.02

6.021 ± 0.021

8.642 ± 0.026

1.478 ± 0.011

1.905 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski