Amino acid dipepetide frequency for Frankia sp. EUN1h

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	25.054AlaAla: 25.054 ± 0.162	1.043AlaCys: 1.043 ± 0.022	9.407AlaAsp: 9.407 ± 0.082	8.01AlaGlu: 8.01 ± 0.07	3.689AlaPhe: 3.689 ± 0.045	15.681AlaGly: 15.681 ± 0.102	2.681AlaHis: 2.681 ± 0.037	4.211AlaIle: 4.211 ± 0.041	2.089AlaLys: 2.089 ± 0.033	14.413AlaLeu: 14.413 ± 0.099	2.539AlaMet: 2.539 ± 0.031	2.128AlaAsn: 2.128 ± 0.031	7.583AlaPro: 7.583 ± 0.077	3.465AlaGln: 3.465 ± 0.038	11.665AlaArg: 11.665 ± 0.088	6.973AlaSer: 6.973 ± 0.055	8.484AlaThr: 8.484 ± 0.067	12.58AlaVal: 12.58 ± 0.09	1.852AlaTrp: 1.852 ± 0.027	2.378AlaTyr: 2.378 ± 0.031	0.101AlaXaa: 0.101 ± 0.006
Cys	1.05CysAla: 1.05 ± 0.023	0.089CysCys: 0.089 ± 0.006	0.47CysAsp: 0.47 ± 0.015	0.345CysGlu: 0.345 ± 0.013	0.233CysPhe: 0.233 ± 0.009	0.892CysGly: 0.892 ± 0.02	0.202CysHis: 0.202 ± 0.009	0.154CysIle: 0.154 ± 0.008	0.077CysLys: 0.077 ± 0.006	0.757CysLeu: 0.757 ± 0.019	0.102CysMet: 0.102 ± 0.006	0.115CysAsn: 0.115 ± 0.006	0.474CysPro: 0.474 ± 0.014	0.215CysGln: 0.215 ± 0.009	0.546CysArg: 0.546 ± 0.014	0.387CysSer: 0.387 ± 0.011	0.346CysThr: 0.346 ± 0.012	0.629CysVal: 0.629 ± 0.017	0.125CysTrp: 0.125 ± 0.007	0.175CysTyr: 0.175 ± 0.009	0.004CysXaa: 0.004 ± 0.001
Asp	8.098AspAla: 8.098 ± 0.062	0.391AspCys: 0.391 ± 0.014	4.256AspAsp: 4.256 ± 0.051	4.064AspGlu: 4.064 ± 0.045	1.508AspPhe: 1.508 ± 0.032	6.763AspGly: 6.763 ± 0.066	1.423AspHis: 1.423 ± 0.025	1.917AspIle: 1.917 ± 0.03	0.831AspLys: 0.831 ± 0.019	7.114AspLeu: 7.114 ± 0.056	0.734AspMet: 0.734 ± 0.018	0.894AspAsn: 0.894 ± 0.022	4.933AspPro: 4.933 ± 0.045	1.816AspGln: 1.816 ± 0.026	5.109AspArg: 5.109 ± 0.051	2.219AspSer: 2.219 ± 0.03	2.873AspThr: 2.873 ± 0.036	5.366AspVal: 5.366 ± 0.05	0.966AspTrp: 0.966 ± 0.02	1.16AspTyr: 1.16 ± 0.019	0.04AspXaa: 0.04 ± 0.004
Glu	6.646GluAla: 6.646 ± 0.061	0.299GluCys: 0.299 ± 0.011	2.316GluAsp: 2.316 ± 0.033	2.348GluGlu: 2.348 ± 0.039	1.386GluPhe: 1.386 ± 0.028	3.207GluGly: 3.207 ± 0.039	1.369GluHis: 1.369 ± 0.024	2.418GluIle: 2.418 ± 0.04	1.001GluLys: 1.001 ± 0.026	6.371GluLeu: 6.371 ± 0.064	0.777GluMet: 0.777 ± 0.019	0.872GluAsn: 0.872 ± 0.018	3.441GluPro: 3.441 ± 0.04	1.862GluGln: 1.862 ± 0.025	5.033GluArg: 5.033 ± 0.053	2.134GluSer: 2.134 ± 0.028	2.733GluThr: 2.733 ± 0.034	4.212GluVal: 4.212 ± 0.045	0.674GluTrp: 0.674 ± 0.018	0.942GluTyr: 0.942 ± 0.023	0.031GluXaa: 0.031 ± 0.003
Phe	3.884PheAla: 3.884 ± 0.046	0.289PheCys: 0.289 ± 0.012	2.11PheAsp: 2.11 ± 0.031	1.364PheGlu: 1.364 ± 0.028	0.824PhePhe: 0.824 ± 0.02	3.158PheGly: 3.158 ± 0.043	0.588PheHis: 0.588 ± 0.015	0.757PheIle: 0.757 ± 0.018	0.356PheLys: 0.356 ± 0.012	2.562PheLeu: 2.562 ± 0.034	0.359PheMet: 0.359 ± 0.012	0.505PheAsn: 0.505 ± 0.016	1.459PhePro: 1.459 ± 0.025	0.691PheGln: 0.691 ± 0.015	1.8PheArg: 1.8 ± 0.028	1.391PheSer: 1.391 ± 0.031	1.873PheThr: 1.873 ± 0.03	2.311PheVal: 2.311 ± 0.031	0.398PheTrp: 0.398 ± 0.013	0.572PheTyr: 0.572 ± 0.016	0.01PheXaa: 0.01 ± 0.002
Gly	11.946GlyAla: 11.946 ± 0.079	0.746GlyCys: 0.746 ± 0.021	5.387GlyAsp: 5.387 ± 0.05	4.606GlyGlu: 4.606 ± 0.048	2.805GlyPhe: 2.805 ± 0.031	9.83GlyGly: 9.83 ± 0.097	2.225GlyHis: 2.225 ± 0.035	3.431GlyIle: 3.431 ± 0.039	1.781GlyLys: 1.781 ± 0.025	9.888GlyLeu: 9.888 ± 0.072	1.841GlyMet: 1.841 ± 0.027	1.533GlyAsn: 1.533 ± 0.027	6.067GlyPro: 6.067 ± 0.064	3.104GlyGln: 3.104 ± 0.036	8.5GlyArg: 8.5 ± 0.076	5.259GlySer: 5.259 ± 0.048	5.81GlyThr: 5.81 ± 0.059	7.721GlyVal: 7.721 ± 0.061	1.852GlyTrp: 1.852 ± 0.031	2.348GlyTyr: 2.348 ± 0.032	0.06GlyXaa: 0.06 ± 0.005
His	2.56HisAla: 2.56 ± 0.037	0.159HisCys: 0.159 ± 0.008	1.355HisAsp: 1.355 ± 0.021	1.05HisGlu: 1.05 ± 0.02	0.57HisPhe: 0.57 ± 0.017	2.22HisGly: 2.22 ± 0.032	0.633HisHis: 0.633 ± 0.018	0.589HisIle: 0.589 ± 0.016	0.23HisLys: 0.23 ± 0.01	2.355HisLeu: 2.355 ± 0.033	0.267HisMet: 0.267 ± 0.011	0.317HisAsn: 0.317 ± 0.01	1.797HisPro: 1.797 ± 0.031	0.638HisGln: 0.638 ± 0.016	1.975HisArg: 1.975 ± 0.029	0.914HisSer: 0.914 ± 0.015	1.115HisThr: 1.115 ± 0.024	1.63HisVal: 1.63 ± 0.028	0.333HisTrp: 0.333 ± 0.011	0.452HisTyr: 0.452 ± 0.013	0.011HisXaa: 0.011 ± 0.002
Ile	4.907IleAla: 4.907 ± 0.05	0.309IleCys: 0.309 ± 0.01	2.458IleAsp: 2.458 ± 0.035	1.958IleGlu: 1.958 ± 0.033	0.835IlePhe: 0.835 ± 0.018	3.621IleGly: 3.621 ± 0.043	0.691IleHis: 0.691 ± 0.014	1.056IleIle: 1.056 ± 0.025	0.548IleLys: 0.548 ± 0.014	2.567IleLeu: 2.567 ± 0.034	0.48IleMet: 0.48 ± 0.015	0.687IleAsn: 0.687 ± 0.017	1.957IlePro: 1.957 ± 0.028	0.772IleGln: 0.772 ± 0.016	2.575IleArg: 2.575 ± 0.034	1.824IleSer: 1.824 ± 0.028	2.143IleThr: 2.143 ± 0.033	2.993IleVal: 2.993 ± 0.035	0.414IleTrp: 0.414 ± 0.013	0.617IleTyr: 0.617 ± 0.015	0.02IleXaa: 0.02 ± 0.003
Lys	2.066LysAla: 2.066 ± 0.033	0.072LysCys: 0.072 ± 0.005	0.865LysAsp: 0.865 ± 0.025	0.726LysGlu: 0.726 ± 0.019	0.346LysPhe: 0.346 ± 0.013	1.196LysGly: 1.196 ± 0.026	0.295LysHis: 0.295 ± 0.01	0.867LysIle: 0.867 ± 0.019	0.386LysLys: 0.386 ± 0.014	1.48LysLeu: 1.48 ± 0.024	0.275LysMet: 0.275 ± 0.011	0.351LysAsn: 0.351 ± 0.012	0.959LysPro: 0.959 ± 0.023	0.41LysGln: 0.41 ± 0.013	0.974LysArg: 0.974 ± 0.02	0.757LysSer: 0.757 ± 0.021	0.909LysThr: 0.909 ± 0.021	1.525LysVal: 1.525 ± 0.029	0.169LysTrp: 0.169 ± 0.009	0.322LysTyr: 0.322 ± 0.014	0.009LysXaa: 0.009 ± 0.002
Leu	17.502LeuAla: 17.502 ± 0.114	0.793LeuCys: 0.793 ± 0.017	7.093LeuAsp: 7.093 ± 0.059	4.305LeuGlu: 4.305 ± 0.047	2.616LeuPhe: 2.616 ± 0.034	9.52LeuGly: 9.52 ± 0.072	1.98LeuHis: 1.98 ± 0.031	3.069LeuIle: 3.069 ± 0.042	1.252LeuLys: 1.252 ± 0.025	10.94LeuLeu: 10.94 ± 0.102	1.403LeuMet: 1.403 ± 0.024	1.471LeuAsn: 1.471 ± 0.025	6.274LeuPro: 6.274 ± 0.052	1.718LeuGln: 1.718 ± 0.026	9.077LeuArg: 9.077 ± 0.074	5.034LeuSer: 5.034 ± 0.056	6.798LeuThr: 6.798 ± 0.055	9.601LeuVal: 9.601 ± 0.076	1.28LeuTrp: 1.28 ± 0.028	1.582LeuTyr: 1.582 ± 0.024	0.068LeuXaa: 0.068 ± 0.005
Met	2.181MetAla: 2.181 ± 0.028	0.131MetCys: 0.131 ± 0.007	0.787MetAsp: 0.787 ± 0.018	0.603MetGlu: 0.603 ± 0.016	0.506MetPhe: 0.506 ± 0.015	1.122MetGly: 1.122 ± 0.02	0.297MetHis: 0.297 ± 0.011	0.741MetIle: 0.741 ± 0.018	0.312MetLys: 0.312 ± 0.012	1.708MetLeu: 1.708 ± 0.025	0.269MetMet: 0.269 ± 0.011	0.357MetAsn: 0.357 ± 0.012	1.088MetPro: 1.088 ± 0.022	0.332MetGln: 0.332 ± 0.011	1.343MetArg: 1.343 ± 0.023	1.154MetSer: 1.154 ± 0.022	1.488MetThr: 1.488 ± 0.024	1.258MetVal: 1.258 ± 0.024	0.196MetTrp: 0.196 ± 0.009	0.265MetTyr: 0.265 ± 0.011	0.015MetXaa: 0.015 ± 0.002
Asn	2.081AsnAla: 2.081 ± 0.031	0.137AsnCys: 0.137 ± 0.008	0.878AsnAsp: 0.878 ± 0.02	0.714AsnGlu: 0.714 ± 0.017	0.418AsnPhe: 0.418 ± 0.013	1.681AsnGly: 1.681 ± 0.028	0.356AsnHis: 0.356 ± 0.012	0.657AsnIle: 0.657 ± 0.016	0.295AsnLys: 0.295 ± 0.012	1.74AsnLeu: 1.74 ± 0.032	0.241AsnMet: 0.241 ± 0.01	0.374AsnAsn: 0.374 ± 0.014	1.36AsnPro: 1.36 ± 0.024	0.498AsnGln: 0.498 ± 0.015	1.249AsnArg: 1.249 ± 0.022	0.784AsnSer: 0.784 ± 0.021	0.94AsnThr: 0.94 ± 0.02	1.399AsnVal: 1.399 ± 0.025	0.244AsnTrp: 0.244 ± 0.011	0.357AsnTyr: 0.357 ± 0.01	0.01AsnXaa: 0.01 ± 0.002
Pro	10.703ProAla: 10.703 ± 0.088	0.301ProCys: 0.301 ± 0.011	5.004ProAsp: 5.004 ± 0.046	3.509ProGlu: 3.509 ± 0.038	1.627ProPhe: 1.627 ± 0.026	7.205ProGly: 7.205 ± 0.068	1.163ProHis: 1.163 ± 0.023	1.635ProIle: 1.635 ± 0.023	0.918ProLys: 0.918 ± 0.018	5.181ProLeu: 5.181 ± 0.051	1.055ProMet: 1.055 ± 0.02	1.03ProAsn: 1.03 ± 0.021	4.72ProPro: 4.72 ± 0.073	1.175ProGln: 1.175 ± 0.023	4.593ProArg: 4.593 ± 0.047	3.548ProSer: 3.548 ± 0.047	3.923ProThr: 3.923 ± 0.048	5.516ProVal: 5.516 ± 0.05	0.882ProTrp: 0.882 ± 0.02	1.128ProTyr: 1.128 ± 0.024	0.038ProXaa: 0.038 ± 0.004
Gln	3.675GlnAla: 3.675 ± 0.032	0.154GlnCys: 0.154 ± 0.008	1.143GlnAsp: 1.143 ± 0.02	1.112GlnGlu: 1.112 ± 0.025	0.702GlnPhe: 0.702 ± 0.016	1.841GlnGly: 1.841 ± 0.025	0.55GlnHis: 0.55 ± 0.015	1.134GlnIle: 1.134 ± 0.022	0.405GlnLys: 0.405 ± 0.015	3.025GlnLeu: 3.025 ± 0.034	0.426GlnMet: 0.426 ± 0.014	0.457GlnAsn: 0.457 ± 0.016	1.836GlnPro: 1.836 ± 0.033	0.903GlnGln: 0.903 ± 0.022	2.264GlnArg: 2.264 ± 0.031	1.109GlnSer: 1.109 ± 0.019	1.339GlnThr: 1.339 ± 0.024	2.49GlnVal: 2.49 ± 0.034	0.364GlnTrp: 0.364 ± 0.011	0.474GlnTyr: 0.474 ± 0.013	0.014GlnXaa: 0.014 ± 0.003
Arg	11.365ArgAla: 11.365 ± 0.09	0.583ArgCys: 0.583 ± 0.019	4.73ArgAsp: 4.73 ± 0.046	4.087ArgGlu: 4.087 ± 0.046	2.53ArgPhe: 2.53 ± 0.033	6.24ArgGly: 6.24 ± 0.055	2.148ArgHis: 2.148 ± 0.032	2.939ArgIle: 2.939 ± 0.035	1.066ArgLys: 1.066 ± 0.022	9.441ArgLeu: 9.441 ± 0.078	1.645ArgMet: 1.645 ± 0.025	1.146ArgAsn: 1.146 ± 0.024	5.775ArgPro: 5.775 ± 0.057	2.426ArgGln: 2.426 ± 0.037	8.716ArgArg: 8.716 ± 0.078	4.044ArgSer: 4.044 ± 0.044	4.568ArgThr: 4.568 ± 0.048	6.387ArgVal: 6.387 ± 0.052	1.519ArgTrp: 1.519 ± 0.025	1.851ArgTyr: 1.851 ± 0.029	0.057ArgXaa: 0.057 ± 0.005
Ser	6.975SerAla: 6.975 ± 0.062	0.386SerCys: 0.386 ± 0.013	2.692SerAsp: 2.692 ± 0.032	2.103SerGlu: 2.103 ± 0.031	1.511SerPhe: 1.511 ± 0.025	5.638SerGly: 5.638 ± 0.048	0.925SerHis: 0.925 ± 0.021	1.562SerIle: 1.562 ± 0.028	0.774SerLys: 0.774 ± 0.018	4.668SerLeu: 4.668 ± 0.044	1.034SerMet: 1.034 ± 0.021	0.838SerAsn: 0.838 ± 0.022	3.666SerPro: 3.666 ± 0.047	1.256SerGln: 1.256 ± 0.024	3.846SerArg: 3.846 ± 0.044	2.864SerSer: 2.864 ± 0.038	3.151SerThr: 3.151 ± 0.036	4.005SerVal: 4.005 ± 0.038	0.898SerTrp: 0.898 ± 0.021	1.064SerTyr: 1.064 ± 0.022	0.036SerXaa: 0.036 ± 0.004
Thr	8.705ThrAla: 8.705 ± 0.061	0.43ThrCys: 0.43 ± 0.013	3.502ThrAsp: 3.502 ± 0.041	2.774ThrGlu: 2.774 ± 0.035	1.774ThrPhe: 1.774 ± 0.033	6.566ThrGly: 6.566 ± 0.053	1.099ThrHis: 1.099 ± 0.019	2.166ThrIle: 2.166 ± 0.032	0.889ThrLys: 0.889 ± 0.021	5.581ThrLeu: 5.581 ± 0.048	1.026ThrMet: 1.026 ± 0.018	1.032ThrAsn: 1.032 ± 0.022	4.152ThrPro: 4.152 ± 0.048	1.284ThrGln: 1.284 ± 0.025	4.105ThrArg: 4.105 ± 0.044	3.378ThrSer: 3.378 ± 0.043	3.897ThrThr: 3.897 ± 0.058	5.727ThrVal: 5.727 ± 0.048	0.892ThrTrp: 0.892 ± 0.019	1.184ThrTyr: 1.184 ± 0.024	0.044ThrXaa: 0.044 ± 0.004
Val	12.57ValAla: 12.57 ± 0.085	0.718ValCys: 0.718 ± 0.018	5.784ValAsp: 5.784 ± 0.049	4.654ValGlu: 4.654 ± 0.051	2.287ValPhe: 2.287 ± 0.03	7.468ValGly: 7.468 ± 0.051	1.743ValHis: 1.743 ± 0.027	3.087ValIle: 3.087 ± 0.037	1.189ValLys: 1.189 ± 0.024	9.401ValLeu: 9.401 ± 0.072	1.186ValMet: 1.186 ± 0.023	1.596ValAsn: 1.596 ± 0.025	5.153ValPro: 5.153 ± 0.049	1.72ValGln: 1.72 ± 0.027	6.653ValArg: 6.653 ± 0.057	4.297ValSer: 4.297 ± 0.041	5.816ValThr: 5.816 ± 0.053	8.551ValVal: 8.551 ± 0.081	1.064ValTrp: 1.064 ± 0.02	1.439ValTyr: 1.439 ± 0.026	0.051ValXaa: 0.051 ± 0.004
Trp	1.667TrpAla: 1.667 ± 0.024	0.167TrpCys: 0.167 ± 0.01	0.921TrpAsp: 0.921 ± 0.023	0.724TrpGlu: 0.724 ± 0.019	0.447TrpPhe: 0.447 ± 0.015	0.939TrpGly: 0.939 ± 0.022	0.363TrpHis: 0.363 ± 0.012	0.523TrpIle: 0.523 ± 0.014	0.281TrpLys: 0.281 ± 0.011	1.849TrpLeu: 1.849 ± 0.026	0.272TrpMet: 0.272 ± 0.01	0.342TrpAsn: 0.342 ± 0.011	0.934TrpPro: 0.934 ± 0.018	0.448TrpGln: 0.448 ± 0.014	1.42TrpArg: 1.42 ± 0.027	0.897TrpSer: 0.897 ± 0.022	0.95TrpThr: 0.95 ± 0.019	1.013TrpVal: 1.013 ± 0.022	0.315TrpTrp: 0.315 ± 0.011	0.3TrpTyr: 0.3 ± 0.01	0.009TrpXaa: 0.009 ± 0.002
Tyr	2.296TyrAla: 2.296 ± 0.028	0.18TyrCys: 0.18 ± 0.008	1.279TyrAsp: 1.279 ± 0.028	0.987TyrGlu: 0.987 ± 0.023	0.629TyrPhe: 0.629 ± 0.016	1.894TyrGly: 1.894 ± 0.033	0.425TyrHis: 0.425 ± 0.013	0.498TyrIle: 0.498 ± 0.016	0.267TyrLys: 0.267 ± 0.01	2.273TyrLeu: 2.273 ± 0.038	0.207TyrMet: 0.207 ± 0.009	0.355TyrAsn: 0.355 ± 0.012	1.183TyrPro: 1.183 ± 0.024	0.665TyrGln: 0.665 ± 0.017	1.771TyrArg: 1.771 ± 0.026	0.895TyrSer: 0.895 ± 0.017	1.005TyrThr: 1.005 ± 0.023	1.458TyrVal: 1.458 ± 0.023	0.338TyrTrp: 0.338 ± 0.013	0.434TyrTyr: 0.434 ± 0.014	0.016TyrXaa: 0.016 ± 0.002
Xaa	0.094XaaAla: 0.094 ± 0.007	0.008XaaCys: 0.008 ± 0.002	0.039XaaAsp: 0.039 ± 0.004	0.031XaaGlu: 0.031 ± 0.003	0.017XaaPhe: 0.017 ± 0.003	0.056XaaGly: 0.056 ± 0.005	0.013XaaHis: 0.013 ± 0.002	0.024XaaIle: 0.024 ± 0.003	0.012XaaLys: 0.012 ± 0.002	0.062XaaLeu: 0.062 ± 0.004	0.014XaaMet: 0.014 ± 0.002	0.013XaaAsn: 0.013 ± 0.002	0.042XaaPro: 0.042 ± 0.004	0.019XaaGln: 0.019 ± 0.003	0.051XaaArg: 0.051 ± 0.005	0.032XaaSer: 0.032 ± 0.004	0.047XaaThr: 0.047 ± 0.005	0.053XaaVal: 0.053 ± 0.005	0.007XaaTrp: 0.007 ± 0.002	0.009XaaTyr: 0.009 ± 0.002	0.004XaaXaa: 0.004 ± 0.002

Statistics based on 7402 proteins (2529610 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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