Paramaledivibacter caminithermalis DSM 15212
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3784 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M6Q1N6|A0A1M6Q1N6_9CLOT L-asparaginase OS=Paramaledivibacter caminithermalis DSM 15212 OX=1121301 GN=SAMN02745912_02381 PE=3 SV=1
MM1 pKa = 7.48 KK2 pKa = 10.05 KK3 pKa = 10.16 YY4 pKa = 10.52 RR5 pKa = 11.84 CIPCGYY11 pKa = 9.88 EE12 pKa = 3.57 YY13 pKa = 10.65 DD14 pKa = 4.1 PQVGDD19 pKa = 4.09 PDD21 pKa = 4.19 SGVAPGTAFEE31 pKa = 5.97 DD32 pKa = 4.28 IPDD35 pKa = 3.7 DD36 pKa = 3.88 WVCPVCGVGKK46 pKa = 10.52 DD47 pKa = 3.17 MFEE50 pKa = 4.09 PVEE53 pKa = 4.0
Molecular weight: 5.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.822
IPC2_protein 3.923
IPC_protein 3.821
Toseland 3.617
ProMoST 3.948
Dawson 3.821
Bjellqvist 4.075
Wikipedia 3.783
Rodwell 3.656
Grimsley 3.541
Solomon 3.795
Lehninger 3.757
Nozaki 3.961
DTASelect 4.164
Thurlkill 3.694
EMBOSS 3.795
Sillero 3.948
Patrickios 0.401
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.915
Protein with the highest isoelectric point:
>tr|A0A1M6NYK6|A0A1M6NYK6_9CLOT Uncharacterized protein family (UPF0180) OS=Paramaledivibacter caminithermalis DSM 15212 OX=1121301 GN=SAMN02745912_01937 PE=4 SV=1
MM1 pKa = 7.74 TYY3 pKa = 10.12 QPKK6 pKa = 9.89 KK7 pKa = 8.23 RR8 pKa = 11.84 QRR10 pKa = 11.84 KK11 pKa = 8.39 KK12 pKa = 8.49 EE13 pKa = 3.5 HH14 pKa = 6.11 GFRR17 pKa = 11.84 KK18 pKa = 9.77 RR19 pKa = 11.84 MKK21 pKa = 8.75 TKK23 pKa = 10.24 SGRR26 pKa = 11.84 NVLKK30 pKa = 10.19 RR31 pKa = 11.84 RR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 KK35 pKa = 8.91 GRR37 pKa = 11.84 KK38 pKa = 9.02 RR39 pKa = 11.84 LTAA42 pKa = 4.18
Molecular weight: 5.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.339
IPC2_protein 10.877
IPC_protein 12.281
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.281
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 12.003
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.005
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3784
0
3784
1112298
39
2372
293.9
33.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.55 ± 0.041
1.059 ± 0.017
5.592 ± 0.031
7.434 ± 0.044
4.352 ± 0.031
6.42 ± 0.041
1.459 ± 0.015
10.65 ± 0.05
9.341 ± 0.046
9.11 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.596 ± 0.018
5.964 ± 0.037
2.924 ± 0.024
2.314 ± 0.019
3.772 ± 0.027
5.814 ± 0.03
4.664 ± 0.024
6.152 ± 0.034
0.708 ± 0.013
4.125 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here