Mycoplasma cynos (strain C142)
Average proteome isoelectric point is 7.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 871 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|L0RUD4|L0RUD4_MYCC1 L-ribulose-5-phosphate 4-epimerase OS=Mycoplasma cynos (strain C142) OX=1246955 GN=MCYN0231 PE=4 SV=1
MM1 pKa = 7.44 NCNTMYY7 pKa = 9.38 KK8 pKa = 9.25 TILGATIEE16 pKa = 4.64 IINDD20 pKa = 4.78 GIVEE24 pKa = 4.21 TDD26 pKa = 3.15 VNAAQMPVVCISTIINEE43 pKa = 4.16 IKK45 pKa = 10.38 IIDD48 pKa = 4.22 HH49 pKa = 6.84 IIINSIWIIWDD60 pKa = 3.14 NSIFF64 pKa = 3.66
Molecular weight: 7.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.865
IPC2_protein 4.19
IPC_protein 3.973
Toseland 3.795
ProMoST 4.164
Dawson 3.973
Bjellqvist 4.139
Wikipedia 3.935
Rodwell 3.821
Grimsley 3.732
Solomon 3.948
Lehninger 3.897
Nozaki 4.126
DTASelect 4.291
Thurlkill 3.884
EMBOSS 3.935
Sillero 4.101
Patrickios 1.952
IPC_peptide 3.948
IPC2_peptide 4.075
IPC2.peptide.svr19 4.003
Protein with the highest isoelectric point:
>tr|L0RWC2|L0RWC2_MYCC1 Uncharacterized protein OS=Mycoplasma cynos (strain C142) OX=1246955 GN=MCYN0133 PE=4 SV=1
MM1 pKa = 7.34 ARR3 pKa = 11.84 KK4 pKa = 9.41 ALIEE8 pKa = 3.88 KK9 pKa = 9.99 SKK11 pKa = 10.28 RR12 pKa = 11.84 HH13 pKa = 5.76 PKK15 pKa = 10.14 FSTRR19 pKa = 11.84 AYY21 pKa = 8.48 TRR23 pKa = 11.84 CEE25 pKa = 3.8 LCGRR29 pKa = 11.84 PHH31 pKa = 7.07 AVLRR35 pKa = 11.84 KK36 pKa = 9.46 YY37 pKa = 10.22 KK38 pKa = 9.85 ICRR41 pKa = 11.84 ICFRR45 pKa = 11.84 NLAHH49 pKa = 6.89 EE50 pKa = 4.48 GKK52 pKa = 10.19 IPGVKK57 pKa = 9.38 KK58 pKa = 10.89 ASWW61 pKa = 3.03
Molecular weight: 7.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.323
IPC2_protein 9.765
IPC_protein 10.248
Toseland 10.877
ProMoST 10.452
Dawson 10.95
Bjellqvist 10.599
Wikipedia 11.096
Rodwell 11.359
Grimsley 10.965
Solomon 11.038
Lehninger 11.023
Nozaki 10.862
DTASelect 10.584
Thurlkill 10.862
EMBOSS 11.257
Sillero 10.877
Patrickios 11.096
IPC_peptide 11.052
IPC2_peptide 9.706
IPC2.peptide.svr19 8.54
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
871
0
871
298842
40
2711
343.1
39.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.669 ± 0.077
0.444 ± 0.02
5.611 ± 0.074
6.855 ± 0.089
5.469 ± 0.075
4.411 ± 0.083
1.36 ± 0.028
10.078 ± 0.097
11.297 ± 0.116
9.412 ± 0.073
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.614 ± 0.035
8.439 ± 0.11
2.628 ± 0.053
3.075 ± 0.047
2.859 ± 0.044
6.68 ± 0.069
4.898 ± 0.048
5.011 ± 0.072
0.913 ± 0.024
4.278 ± 0.062
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here