Amino acid dipepetide frequency for Mycoplasma cynos (strain C142)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.399AlaAla: 2.399 ± 0.117
0.298AlaCys: 0.298 ± 0.032
2.175AlaAsp: 2.175 ± 0.099
2.627AlaGlu: 2.627 ± 0.121
2.272AlaPhe: 2.272 ± 0.104
2.229AlaGly: 2.229 ± 0.112
0.693AlaHis: 0.693 ± 0.055
5.217AlaIle: 5.217 ± 0.165
6.03AlaLys: 6.03 ± 0.205
4.708AlaLeu: 4.708 ± 0.147
0.719AlaMet: 0.719 ± 0.054
3.403AlaAsn: 3.403 ± 0.112
1.188AlaPro: 1.188 ± 0.072
1.442AlaGln: 1.442 ± 0.083
1.727AlaArg: 1.727 ± 0.089
3.065AlaSer: 3.065 ± 0.113
2.48AlaThr: 2.48 ± 0.096
1.981AlaVal: 1.981 ± 0.104
0.335AlaTrp: 0.335 ± 0.034
1.7AlaTyr: 1.7 ± 0.075
0.0AlaXaa: 0.0 ± 0.0
Cys
0.315CysAla: 0.315 ± 0.03
0.037CysCys: 0.037 ± 0.011
0.291CysAsp: 0.291 ± 0.036
0.261CysGlu: 0.261 ± 0.037
0.254CysPhe: 0.254 ± 0.028
0.268CysGly: 0.268 ± 0.036
0.087CysHis: 0.087 ± 0.017
0.452CysIle: 0.452 ± 0.033
0.301CysLys: 0.301 ± 0.032
0.482CysLeu: 0.482 ± 0.041
0.07CysMet: 0.07 ± 0.015
0.291CysAsn: 0.291 ± 0.03
0.117CysPro: 0.117 ± 0.02
0.127CysGln: 0.127 ± 0.021
0.141CysArg: 0.141 ± 0.021
0.402CysSer: 0.402 ± 0.041
0.167CysThr: 0.167 ± 0.024
0.207CysVal: 0.207 ± 0.025
0.037CysTrp: 0.037 ± 0.01
0.134CysTyr: 0.134 ± 0.021
0.0CysXaa: 0.0 ± 0.0
Asp
2.958AspAla: 2.958 ± 0.117
0.171AspCys: 0.171 ± 0.025
3.176AspAsp: 3.176 ± 0.124
4.611AspGlu: 4.611 ± 0.161
3.744AspPhe: 3.744 ± 0.119
2.547AspGly: 2.547 ± 0.109
0.793AspHis: 0.793 ± 0.058
5.22AspIle: 5.22 ± 0.139
5.986AspLys: 5.986 ± 0.184
5.474AspLeu: 5.474 ± 0.165
0.562AspMet: 0.562 ± 0.045
4.046AspAsn: 4.046 ± 0.162
1.533AspPro: 1.533 ± 0.077
2.008AspGln: 2.008 ± 0.095
1.332AspArg: 1.332 ± 0.067
3.239AspSer: 3.239 ± 0.13
2.098AspThr: 2.098 ± 0.09
3.269AspVal: 3.269 ± 0.106
0.525AspTrp: 0.525 ± 0.045
2.821AspTyr: 2.821 ± 0.115
0.0AspXaa: 0.0 ± 0.0
Glu
3.199GluAla: 3.199 ± 0.132
0.201GluCys: 0.201 ± 0.028
2.536GluAsp: 2.536 ± 0.108
4.173GluGlu: 4.173 ± 0.163
4.113GluPhe: 4.113 ± 0.133
2.232GluGly: 2.232 ± 0.101
0.92GluHis: 0.92 ± 0.059
7.994GluIle: 7.994 ± 0.181
8.717GluLys: 8.717 ± 0.227
7.653GluLeu: 7.653 ± 0.19
1.211GluMet: 1.211 ± 0.073
6.033GluAsn: 6.033 ± 0.179
1.332GluPro: 1.332 ± 0.073
2.366GluGln: 2.366 ± 0.107
1.877GluArg: 1.877 ± 0.079
3.276GluSer: 3.276 ± 0.111
3.37GluThr: 3.37 ± 0.116
3.584GluVal: 3.584 ± 0.136
0.525GluTrp: 0.525 ± 0.039
3.243GluTyr: 3.243 ± 0.111
0.0GluXaa: 0.0 ± 0.0
Phe
2.48PheAla: 2.48 ± 0.096
0.355PheCys: 0.355 ± 0.035
3.744PheAsp: 3.744 ± 0.134
3.601PheGlu: 3.601 ± 0.115
2.891PhePhe: 2.891 ± 0.144
2.777PheGly: 2.777 ± 0.113
0.552PheHis: 0.552 ± 0.051
5.635PheIle: 5.635 ± 0.184
5.719PheLys: 5.719 ± 0.17
5.264PheLeu: 5.264 ± 0.169
0.813PheMet: 0.813 ± 0.05
4.564PheAsn: 4.564 ± 0.141
1.275PhePro: 1.275 ± 0.069
1.262PheGln: 1.262 ± 0.069
1.298PheArg: 1.298 ± 0.07
4.26PheSer: 4.26 ± 0.144
2.359PheThr: 2.359 ± 0.103
2.838PheVal: 2.838 ± 0.097
0.706PheTrp: 0.706 ± 0.045
2.296PheTyr: 2.296 ± 0.107
0.0PheXaa: 0.0 ± 0.0
Gly
2.413GlyAla: 2.413 ± 0.121
0.207GlyCys: 0.207 ± 0.029
2.111GlyAsp: 2.111 ± 0.082
2.222GlyGlu: 2.222 ± 0.096
2.808GlyPhe: 2.808 ± 0.111
2.54GlyGly: 2.54 ± 0.131
0.78GlyHis: 0.78 ± 0.062
4.688GlyIle: 4.688 ± 0.18
4.353GlyLys: 4.353 ± 0.134
4.133GlyLeu: 4.133 ± 0.13
0.843GlyMet: 0.843 ± 0.058
2.536GlyAsn: 2.536 ± 0.102
0.927GlyPro: 0.927 ± 0.068
1.308GlyGln: 1.308 ± 0.071
1.593GlyArg: 1.593 ± 0.086
3.058GlySer: 3.058 ± 0.097
2.53GlyThr: 2.53 ± 0.09
2.617GlyVal: 2.617 ± 0.108
0.422GlyTrp: 0.422 ± 0.039
2.014GlyTyr: 2.014 ± 0.096
0.003GlyXaa: 0.003 ± 0.004
His
0.502HisAla: 0.502 ± 0.043
0.084HisCys: 0.084 ± 0.016
0.542HisAsp: 0.542 ± 0.039
0.796HisGlu: 0.796 ± 0.051
0.877HisPhe: 0.877 ± 0.061
0.683HisGly: 0.683 ± 0.047
0.281HisHis: 0.281 ± 0.033
1.372HisIle: 1.372 ± 0.065
1.536HisLys: 1.536 ± 0.075
1.258HisLeu: 1.258 ± 0.069
0.187HisMet: 0.187 ± 0.028
1.292HisAsn: 1.292 ± 0.073
0.485HisPro: 0.485 ± 0.04
0.495HisGln: 0.495 ± 0.042
0.381HisArg: 0.381 ± 0.037
0.96HisSer: 0.96 ± 0.062
0.703HisThr: 0.703 ± 0.045
0.522HisVal: 0.522 ± 0.041
0.12HisTrp: 0.12 ± 0.02
0.519HisTyr: 0.519 ± 0.041
0.0HisXaa: 0.0 ± 0.0
Ile
4.872IleAla: 4.872 ± 0.129
0.525IleCys: 0.525 ± 0.046
6.515IleAsp: 6.515 ± 0.194
6.505IleGlu: 6.505 ± 0.144
5.769IlePhe: 5.769 ± 0.189
4.3IleGly: 4.3 ± 0.166
1.195IleHis: 1.195 ± 0.068
9.681IleIle: 9.681 ± 0.245
11.498IleLys: 11.498 ± 0.227
9.042IleLeu: 9.042 ± 0.24
1.579IleMet: 1.579 ± 0.08
9.022IleAsn: 9.022 ± 0.224
2.777IlePro: 2.777 ± 0.095
2.717IleGln: 2.717 ± 0.09
2.513IleArg: 2.513 ± 0.101
7.854IleSer: 7.854 ± 0.206
4.604IleThr: 4.604 ± 0.133
4.902IleVal: 4.902 ± 0.146
0.827IleTrp: 0.827 ± 0.055
4.079IleTyr: 4.079 ± 0.153
0.0IleXaa: 0.0 ± 0.0
Lys
5.019LysAla: 5.019 ± 0.183
0.371LysCys: 0.371 ± 0.036
6.763LysAsp: 6.763 ± 0.24
9.075LysGlu: 9.075 ± 0.233
4.735LysPhe: 4.735 ± 0.138
3.738LysGly: 3.738 ± 0.123
1.462LysHis: 1.462 ± 0.072
12.003LysIle: 12.003 ± 0.249
12.11LysLys: 12.11 ± 0.286
10.099LysLeu: 10.099 ± 0.215
2.369LysMet: 2.369 ± 0.104
10.698LysAsn: 10.698 ± 0.249
2.831LysPro: 2.831 ± 0.129
3.45LysGln: 3.45 ± 0.137
3.336LysArg: 3.336 ± 0.104
6.264LysSer: 6.264 ± 0.135
6.502LysThr: 6.502 ± 0.163
5.454LysVal: 5.454 ± 0.142
1.011LysTrp: 1.011 ± 0.059
5.675LysTyr: 5.675 ± 0.166
0.0LysXaa: 0.0 ± 0.0
Leu
4.43LeuAla: 4.43 ± 0.146
0.495LeuCys: 0.495 ± 0.042
5.498LeuAsp: 5.498 ± 0.168
6.425LeuGlu: 6.425 ± 0.188
5.247LeuPhe: 5.247 ± 0.166
4.303LeuGly: 4.303 ± 0.127
1.158LeuHis: 1.158 ± 0.072
9.376LeuIle: 9.376 ± 0.224
10.852LeuLys: 10.852 ± 0.21
8.202LeuLeu: 8.202 ± 0.166
1.593LeuMet: 1.593 ± 0.066
8.894LeuAsn: 8.894 ± 0.187
2.526LeuPro: 2.526 ± 0.093
2.322LeuGln: 2.322 ± 0.082
2.66LeuArg: 2.66 ± 0.102
7.01LeuSer: 7.01 ± 0.153
4.39LeuThr: 4.39 ± 0.128
4.748LeuVal: 4.748 ± 0.133
0.79LeuTrp: 0.79 ± 0.058
3.202LeuTyr: 3.202 ± 0.115
0.0LeuXaa: 0.0 ± 0.0
Met
0.649MetAla: 0.649 ± 0.05
0.09MetCys: 0.09 ± 0.017
0.79MetAsp: 0.79 ± 0.051
0.753MetGlu: 0.753 ± 0.061
1.011MetPhe: 1.011 ± 0.059
0.579MetGly: 0.579 ± 0.041
0.261MetHis: 0.261 ± 0.027
1.687MetIle: 1.687 ± 0.066
1.79MetLys: 1.79 ± 0.077
1.663MetLeu: 1.663 ± 0.078
0.351MetMet: 0.351 ± 0.035
1.328MetAsn: 1.328 ± 0.059
0.572MetPro: 0.572 ± 0.052
0.609MetGln: 0.609 ± 0.046
0.522MetArg: 0.522 ± 0.046
1.198MetSer: 1.198 ± 0.071
0.713MetThr: 0.713 ± 0.049
0.87MetVal: 0.87 ± 0.052
0.154MetTrp: 0.154 ± 0.02
0.549MetTyr: 0.549 ± 0.041
0.0MetXaa: 0.0 ± 0.0
Asn
3.54AsnAla: 3.54 ± 0.121
0.234AsnCys: 0.234 ± 0.029
5.381AsnAsp: 5.381 ± 0.145
6.803AsnGlu: 6.803 ± 0.17
4.016AsnPhe: 4.016 ± 0.134
3.467AsnGly: 3.467 ± 0.126
1.101AsnHis: 1.101 ± 0.06
8.279AsnIle: 8.279 ± 0.193
9.798AsnLys: 9.798 ± 0.259
7.569AsnLeu: 7.569 ± 0.19
1.047AsnMet: 1.047 ± 0.058
7.777AsnAsn: 7.777 ± 0.214
2.577AsnPro: 2.577 ± 0.093
3.048AsnGln: 3.048 ± 0.12
1.907AsnArg: 1.907 ± 0.083
5.725AsnSer: 5.725 ± 0.171
3.694AsnThr: 3.694 ± 0.103
4.082AsnVal: 4.082 ± 0.123
0.853AsnTrp: 0.853 ± 0.06
3.493AsnTyr: 3.493 ± 0.124
0.0AsnXaa: 0.0 ± 0.0
Pro
1.131ProAla: 1.131 ± 0.061
0.1ProCys: 0.1 ± 0.02
1.436ProAsp: 1.436 ± 0.073
2.285ProGlu: 2.285 ± 0.109
1.405ProPhe: 1.405 ± 0.07
1.426ProGly: 1.426 ± 0.075
0.435ProHis: 0.435 ± 0.037
2.386ProIle: 2.386 ± 0.098
2.985ProLys: 2.985 ± 0.127
2.081ProLeu: 2.081 ± 0.08
0.341ProMet: 0.341 ± 0.033
2.205ProAsn: 2.205 ± 0.087
0.552ProPro: 0.552 ± 0.052
0.8ProGln: 0.8 ± 0.058
0.703ProArg: 0.703 ± 0.059
1.824ProSer: 1.824 ± 0.09
1.489ProThr: 1.489 ± 0.072
1.185ProVal: 1.185 ± 0.074
0.281ProTrp: 0.281 ± 0.029
1.231ProTyr: 1.231 ± 0.078
0.0ProXaa: 0.0 ± 0.0
Gln
1.432GlnAla: 1.432 ± 0.074
0.09GlnCys: 0.09 ± 0.017
1.426GlnAsp: 1.426 ± 0.069
2.322GlnGlu: 2.322 ± 0.106
1.215GlnPhe: 1.215 ± 0.072
1.221GlnGly: 1.221 ± 0.07
0.335GlnHis: 0.335 ± 0.031
3.115GlnIle: 3.115 ± 0.116
4.384GlnLys: 4.384 ± 0.155
2.55GlnLeu: 2.55 ± 0.089
0.609GlnMet: 0.609 ± 0.048
3.162GlnAsn: 3.162 ± 0.112
0.686GlnPro: 0.686 ± 0.05
0.97GlnGln: 0.97 ± 0.068
1.138GlnArg: 1.138 ± 0.071
1.797GlnSer: 1.797 ± 0.074
1.533GlnThr: 1.533 ± 0.073
1.359GlnVal: 1.359 ± 0.072
0.231GlnTrp: 0.231 ± 0.03
1.168GlnTyr: 1.168 ± 0.063
0.0GlnXaa: 0.0 ± 0.0
Arg
1.285ArgAla: 1.285 ± 0.074
0.094ArgCys: 0.094 ± 0.017
1.436ArgAsp: 1.436 ± 0.077
1.854ArgGlu: 1.854 ± 0.079
1.375ArgPhe: 1.375 ± 0.068
1.228ArgGly: 1.228 ± 0.082
0.418ArgHis: 0.418 ± 0.039
3.005ArgIle: 3.005 ± 0.123
3.135ArgLys: 3.135 ± 0.117
2.647ArgLeu: 2.647 ± 0.105
0.509ArgMet: 0.509 ± 0.046
2.105ArgAsn: 2.105 ± 0.096
0.79ArgPro: 0.79 ± 0.058
0.987ArgGln: 0.987 ± 0.067
1.218ArgArg: 1.218 ± 0.08
1.82ArgSer: 1.82 ± 0.074
1.502ArgThr: 1.502 ± 0.064
1.626ArgVal: 1.626 ± 0.077
0.264ArgTrp: 0.264 ± 0.029
1.285ArgTyr: 1.285 ± 0.075
0.003ArgXaa: 0.003 ± 0.003
Ser
2.761SerAla: 2.761 ± 0.111
0.412SerCys: 0.412 ± 0.042
3.534SerAsp: 3.534 ± 0.116
4.404SerGlu: 4.404 ± 0.12
4.169SerPhe: 4.169 ± 0.14
3.386SerGly: 3.386 ± 0.117
0.94SerHis: 0.94 ± 0.066
6.318SerIle: 6.318 ± 0.153
7.653SerLys: 7.653 ± 0.163
6.609SerLeu: 6.609 ± 0.161
1.111SerMet: 1.111 ± 0.058
5.264SerAsn: 5.264 ± 0.135
1.462SerPro: 1.462 ± 0.092
1.991SerGln: 1.991 ± 0.078
1.834SerArg: 1.834 ± 0.081
4.966SerSer: 4.966 ± 0.156
3.427SerThr: 3.427 ± 0.117
3.172SerVal: 3.172 ± 0.119
0.559SerTrp: 0.559 ± 0.048
2.828SerTyr: 2.828 ± 0.102
0.0SerXaa: 0.0 ± 0.0
Thr
2.024ThrAla: 2.024 ± 0.075
0.181ThrCys: 0.181 ± 0.027
2.346ThrAsp: 2.346 ± 0.088
2.831ThrGlu: 2.831 ± 0.102
2.804ThrPhe: 2.804 ± 0.105
2.309ThrGly: 2.309 ± 0.095
0.756ThrHis: 0.756 ± 0.055
5.197ThrIle: 5.197 ± 0.14
5.521ThrLys: 5.521 ± 0.154
4.829ThrLeu: 4.829 ± 0.125
0.703ThrMet: 0.703 ± 0.049
4.156ThrAsn: 4.156 ± 0.174
1.787ThrPro: 1.787 ± 0.111
1.499ThrGln: 1.499 ± 0.08
1.479ThrArg: 1.479 ± 0.066
3.376ThrSer: 3.376 ± 0.117
2.717ThrThr: 2.717 ± 0.099
2.118ThrVal: 2.118 ± 0.108
0.445ThrTrp: 0.445 ± 0.041
1.904ThrTyr: 1.904 ± 0.081
0.0ThrXaa: 0.0 ± 0.0
Val
2.858ValAla: 2.858 ± 0.095
0.261ValCys: 0.261 ± 0.031
3.102ValAsp: 3.102 ± 0.105
3.413ValGlu: 3.413 ± 0.137
2.848ValPhe: 2.848 ± 0.107
2.547ValGly: 2.547 ± 0.113
0.653ValHis: 0.653 ± 0.05
4.434ValIle: 4.434 ± 0.137
4.966ValLys: 4.966 ± 0.134
4.805ValLeu: 4.805 ± 0.127
0.827ValMet: 0.827 ± 0.059
3.577ValAsn: 3.577 ± 0.096
1.516ValPro: 1.516 ± 0.073
1.422ValGln: 1.422 ± 0.073
1.365ValArg: 1.365 ± 0.069
3.47ValSer: 3.47 ± 0.099
2.429ValThr: 2.429 ± 0.085
3.045ValVal: 3.045 ± 0.126
0.435ValTrp: 0.435 ± 0.046
2.135ValTyr: 2.135 ± 0.084
0.0ValXaa: 0.0 ± 0.0
Trp
0.458TrpAla: 0.458 ± 0.041
0.057TrpCys: 0.057 ± 0.014
0.482TrpAsp: 0.482 ± 0.038
0.535TrpGlu: 0.535 ± 0.054
0.586TrpPhe: 0.586 ± 0.052
0.448TrpGly: 0.448 ± 0.038
0.087TrpHis: 0.087 ± 0.017
0.96TrpIle: 0.96 ± 0.054
0.94TrpLys: 0.94 ± 0.06
0.77TrpLeu: 0.77 ± 0.05
0.144TrpMet: 0.144 ± 0.022
0.74TrpAsn: 0.74 ± 0.055
0.184TrpPro: 0.184 ± 0.026
0.194TrpGln: 0.194 ± 0.023
0.274TrpArg: 0.274 ± 0.028
0.545TrpSer: 0.545 ± 0.046
0.539TrpThr: 0.539 ± 0.047
0.539TrpVal: 0.539 ± 0.048
0.127TrpTrp: 0.127 ± 0.02
0.519TrpTyr: 0.519 ± 0.046
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.961TyrAla: 1.961 ± 0.077
0.177TyrCys: 0.177 ± 0.029
2.831TyrAsp: 2.831 ± 0.114
3.058TyrGlu: 3.058 ± 0.108
2.55TyrPhe: 2.55 ± 0.101
1.881TyrGly: 1.881 ± 0.089
0.689TyrHis: 0.689 ± 0.046
3.758TyrIle: 3.758 ± 0.118
4.688TyrLys: 4.688 ± 0.145
4.287TyrLeu: 4.287 ± 0.146
0.545TyrMet: 0.545 ± 0.042
3.299TyrAsn: 3.299 ± 0.123
1.164TyrPro: 1.164 ± 0.069
1.723TyrGln: 1.723 ± 0.077
1.288TyrArg: 1.288 ± 0.081
2.687TyrSer: 2.687 ± 0.111
1.737TyrThr: 1.737 ± 0.077
1.988TyrVal: 1.988 ± 0.089
0.482TyrTrp: 0.482 ± 0.039
1.988TyrTyr: 1.988 ± 0.115
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.003XaaLys: 0.003 ± 0.003
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.003XaaGln: 0.003 ± 0.004
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 871 proteins (298842 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski