Stomatobaculum longum
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2070 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H1HT06|H1HT06_9FIRM Probable tRNA sulfurtransferase OS=Stomatobaculum longum OX=796942 GN=thiI PE=3 SV=1
MM1 pKa = 7.57 EE2 pKa = 4.44 EE3 pKa = 4.03 EE4 pKa = 4.06 KK5 pKa = 10.83 RR6 pKa = 11.84 EE7 pKa = 4.08 PEE9 pKa = 3.91 AFRR12 pKa = 11.84 AASLVVRR19 pKa = 11.84 DD20 pKa = 4.09 EE21 pKa = 4.1 NSEE24 pKa = 4.11 EE25 pKa = 3.83 VTLYY29 pKa = 10.37 ILEE32 pKa = 4.12 EE33 pKa = 4.08 TRR35 pKa = 11.84 LNGCSYY41 pKa = 10.82 ILAVDD46 pKa = 3.89 SEE48 pKa = 4.44 EE49 pKa = 4.2 ADD51 pKa = 3.54 EE52 pKa = 6.47 DD53 pKa = 4.45 GACYY57 pKa = 10.36 LLKK60 pKa = 10.66 DD61 pKa = 3.78 VSGADD66 pKa = 3.58 DD67 pKa = 3.99 AEE69 pKa = 4.09 AVYY72 pKa = 10.59 EE73 pKa = 4.06 FVEE76 pKa = 4.27 NEE78 pKa = 3.84 EE79 pKa = 3.98 EE80 pKa = 3.77 AGYY83 pKa = 9.04 MLEE86 pKa = 3.96 IFQTILEE93 pKa = 4.74 DD94 pKa = 3.28 EE95 pKa = 4.73 GADD98 pKa = 3.98 IDD100 pKa = 5.51 LLDD103 pKa = 3.91 EE104 pKa = 4.43
Molecular weight: 11.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.854
IPC2_protein 3.592
IPC_protein 3.541
Toseland 3.363
ProMoST 3.656
Dawson 3.503
Bjellqvist 3.706
Wikipedia 3.401
Rodwell 3.376
Grimsley 3.274
Solomon 3.478
Lehninger 3.439
Nozaki 3.63
DTASelect 3.745
Thurlkill 3.401
EMBOSS 3.414
Sillero 3.656
Patrickios 1.748
IPC_peptide 3.49
IPC2_peptide 3.63
IPC2.peptide.svr19 3.657
Protein with the highest isoelectric point:
>tr|H1HW81|H1HW81_9FIRM ABC transporter domain-containing protein OS=Stomatobaculum longum OX=796942 GN=HMPREF9623_01860 PE=4 SV=1
MM1 pKa = 7.08 SAGRR5 pKa = 11.84 GRR7 pKa = 11.84 SALVFLFLGLALLLLFYY24 pKa = 10.49 WNLAAGTVLFSPGKK38 pKa = 9.78 LWALLSTPGSKK49 pKa = 10.61 DD50 pKa = 2.99 PAASILFKK58 pKa = 10.62 IRR60 pKa = 11.84 LPRR63 pKa = 11.84 ALMALLLGGALAVSGFLLQTYY84 pKa = 7.7 FANPIAGPFVLGVSSGARR102 pKa = 11.84 LAVAICMMLFFARR115 pKa = 11.84 NRR117 pKa = 11.84 ATSSLALVLAAFLGSLVSLGFLLAVSSRR145 pKa = 11.84 VRR147 pKa = 11.84 HH148 pKa = 4.78 MAALLVAGIMIGYY161 pKa = 8.35 ICSAATDD168 pKa = 4.08 FLLSFAADD176 pKa = 3.6 SEE178 pKa = 4.61 IVSLHH183 pKa = 5.82 SWSQGSFSGMSLAEE197 pKa = 3.91 VRR199 pKa = 11.84 TAALLIAGCAVALLFLAKK217 pKa = 10.19 PLGAVQLGEE226 pKa = 4.54 SYY228 pKa = 11.34 AEE230 pKa = 3.92 SLGVNVMRR238 pKa = 11.84 LRR240 pKa = 11.84 TLLILLASLLAATVTAFAGPVSFVGVAAPFLIQRR274 pKa = 11.84 LLCTNKK280 pKa = 9.71 PQLVLPASFLGGAVFCMASDD300 pKa = 4.52 FIARR304 pKa = 11.84 TAFQPTEE311 pKa = 3.97 LALSTVTSFFGAPIVLWLLVKK332 pKa = 10.6 RR333 pKa = 11.84 RR334 pKa = 11.84 GKK336 pKa = 10.34
Molecular weight: 35.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 9.619
IPC_protein 10.335
Toseland 10.54
ProMoST 10.292
Dawson 10.672
Bjellqvist 10.394
Wikipedia 10.862
Rodwell 10.891
Grimsley 10.716
Solomon 10.76
Lehninger 10.73
Nozaki 10.57
DTASelect 10.365
Thurlkill 10.555
EMBOSS 10.95
Sillero 10.599
Patrickios 10.613
IPC_peptide 10.76
IPC2_peptide 9.633
IPC2.peptide.svr19 8.498
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2070
0
2070
680711
32
3615
328.8
36.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.772 ± 0.075
1.322 ± 0.024
5.176 ± 0.042
7.75 ± 0.078
4.097 ± 0.038
7.508 ± 0.052
1.745 ± 0.023
5.682 ± 0.051
5.556 ± 0.049
10.168 ± 0.085
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.568 ± 0.029
3.553 ± 0.043
3.744 ± 0.037
3.168 ± 0.026
6.052 ± 0.063
5.714 ± 0.04
5.276 ± 0.059
6.645 ± 0.036
0.879 ± 0.021
3.623 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here