Flavobacterium sp. KMS

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium; unclassified Flavobacterium

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4503 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C1DJB6|A0A0C1DJB6_9FLAO Lycopene cyclase OS=Flavobacterium sp. KMS OX=1566023 GN=OA93_12105 PE=4 SV=1
MM1 pKa = 7.39NLSQEE6 pKa = 3.95DD7 pKa = 3.66WVAQFEE13 pKa = 4.23ADD15 pKa = 3.63EE16 pKa = 4.34NAVILDD22 pKa = 3.25VRR24 pKa = 11.84TEE26 pKa = 3.82DD27 pKa = 4.22EE28 pKa = 4.45YY29 pKa = 11.63NDD31 pKa = 4.5GYY33 pKa = 10.75IEE35 pKa = 4.11NALNIDD41 pKa = 3.72INKK44 pKa = 8.77GQAFIYY50 pKa = 10.14EE51 pKa = 4.3LEE53 pKa = 4.2EE54 pKa = 4.06LDD56 pKa = 4.07KK57 pKa = 11.64NKK59 pKa = 10.6NYY61 pKa = 10.59YY62 pKa = 9.51VYY64 pKa = 9.78CRR66 pKa = 11.84SGARR70 pKa = 11.84SAKK73 pKa = 9.79ACQIMNEE80 pKa = 4.09MGFDD84 pKa = 3.23NAYY87 pKa = 10.55NLLGGILDD95 pKa = 3.82WDD97 pKa = 4.52GEE99 pKa = 4.97TVTPP103 pKa = 4.98

Molecular weight: 11.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C1G595|A0A0C1G595_9FLAO Uncharacterized protein OS=Flavobacterium sp. KMS OX=1566023 GN=OA93_06035 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.91HH17 pKa = 4.39GFMDD21 pKa = 4.5RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.19GRR40 pKa = 11.84HH41 pKa = 5.41KK42 pKa = 10.14LTVSSEE48 pKa = 3.92PRR50 pKa = 11.84HH51 pKa = 5.77KK52 pKa = 10.61KK53 pKa = 9.84

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4503

0

4503

1523331

33

5426

338.3

38.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.286 ± 0.038

0.78 ± 0.013

5.38 ± 0.026

6.236 ± 0.038

5.288 ± 0.027

6.219 ± 0.037

1.566 ± 0.016

8.421 ± 0.038

8.248 ± 0.046

9.123 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.105 ± 0.018

6.522 ± 0.038

3.357 ± 0.021

3.332 ± 0.022

3.004 ± 0.024

6.651 ± 0.03

6.11 ± 0.049

6.083 ± 0.031

1.026 ± 0.013

4.263 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski