Amino acid dipepetide frequency for Flavobacterium sp. KMS

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.181AlaAla: 4.181 ± 0.078	0.513AlaCys: 0.513 ± 0.022	3.204AlaAsp: 3.204 ± 0.064	3.662AlaGlu: 3.662 ± 0.053	3.219AlaPhe: 3.219 ± 0.051	4.09AlaGly: 4.09 ± 0.069	0.944AlaHis: 0.944 ± 0.025	5.672AlaIle: 5.672 ± 0.075	5.009AlaLys: 5.009 ± 0.079	6.064AlaLeu: 6.064 ± 0.075	1.455AlaMet: 1.455 ± 0.037	3.642AlaAsn: 3.642 ± 0.054	1.914AlaPro: 1.914 ± 0.04	2.397AlaGln: 2.397 ± 0.04	1.705AlaArg: 1.705 ± 0.034	4.201AlaSer: 4.201 ± 0.068	4.098AlaThr: 4.098 ± 0.078	3.883AlaVal: 3.883 ± 0.061	0.599AlaTrp: 0.599 ± 0.02	2.403AlaTyr: 2.403 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	0.436CysAla: 0.436 ± 0.02	0.108CysCys: 0.108 ± 0.01	0.432CysAsp: 0.432 ± 0.018	0.462CysGlu: 0.462 ± 0.021	0.452CysPhe: 0.452 ± 0.018	0.62CysGly: 0.62 ± 0.023	0.172CysHis: 0.172 ± 0.012	0.644CysIle: 0.644 ± 0.024	0.534CysLys: 0.534 ± 0.019	0.648CysLeu: 0.648 ± 0.022	0.143CysMet: 0.143 ± 0.01	0.477CysAsn: 0.477 ± 0.021	0.332CysPro: 0.332 ± 0.017	0.216CysGln: 0.216 ± 0.011	0.186CysArg: 0.186 ± 0.012	0.624CysSer: 0.624 ± 0.025	0.492CysThr: 0.492 ± 0.017	0.441CysVal: 0.441 ± 0.019	0.074CysTrp: 0.074 ± 0.007	0.311CysTyr: 0.311 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	3.572AspAla: 3.572 ± 0.065	0.429AspCys: 0.429 ± 0.02	2.609AspAsp: 2.609 ± 0.054	3.395AspGlu: 3.395 ± 0.049	3.611AspPhe: 3.611 ± 0.049	3.514AspGly: 3.514 ± 0.063	0.71AspHis: 0.71 ± 0.022	4.225AspIle: 4.225 ± 0.053	4.711AspLys: 4.711 ± 0.067	5.16AspLeu: 5.16 ± 0.064	1.006AspMet: 1.006 ± 0.028	3.39AspAsn: 3.39 ± 0.06	1.633AspPro: 1.633 ± 0.039	1.458AspGln: 1.458 ± 0.038	1.579AspArg: 1.579 ± 0.033	3.229AspSer: 3.229 ± 0.054	2.61AspThr: 2.61 ± 0.04	3.442AspVal: 3.442 ± 0.054	0.694AspTrp: 0.694 ± 0.025	2.821AspTyr: 2.821 ± 0.051	0.0AspXaa: 0.0 ± 0.0
Glu	3.936GluAla: 3.936 ± 0.065	0.344GluCys: 0.344 ± 0.016	3.083GluAsp: 3.083 ± 0.051	4.077GluGlu: 4.077 ± 0.068	2.867GluPhe: 2.867 ± 0.049	3.24GluGly: 3.24 ± 0.053	0.926GluHis: 0.926 ± 0.026	5.831GluIle: 5.831 ± 0.065	6.185GluLys: 6.185 ± 0.085	5.774GluLeu: 5.774 ± 0.081	1.488GluMet: 1.488 ± 0.034	4.807GluAsn: 4.807 ± 0.064	1.485GluPro: 1.485 ± 0.032	2.075GluGln: 2.075 ± 0.042	2.115GluArg: 2.115 ± 0.039	3.452GluSer: 3.452 ± 0.048	3.664GluThr: 3.664 ± 0.054	3.888GluVal: 3.888 ± 0.063	0.611GluTrp: 0.611 ± 0.021	2.514GluTyr: 2.514 ± 0.047	0.0GluXaa: 0.0 ± 0.0
Phe	3.095PheAla: 3.095 ± 0.051	0.465PheCys: 0.465 ± 0.019	3.221PheAsp: 3.221 ± 0.051	3.305PheGlu: 3.305 ± 0.052	2.894PhePhe: 2.894 ± 0.053	3.603PheGly: 3.603 ± 0.057	0.804PheHis: 0.804 ± 0.026	4.207PheIle: 4.207 ± 0.066	3.836PheLys: 3.836 ± 0.055	4.84PheLeu: 4.84 ± 0.074	1.137PheMet: 1.137 ± 0.028	3.198PheAsn: 3.198 ± 0.057	1.761PhePro: 1.761 ± 0.032	1.541PheGln: 1.541 ± 0.027	1.513PheArg: 1.513 ± 0.03	4.159PheSer: 4.159 ± 0.058	3.282PheThr: 3.282 ± 0.061	3.088PheVal: 3.088 ± 0.053	0.571PheTrp: 0.571 ± 0.022	2.363PheTyr: 2.363 ± 0.035	0.0PheXaa: 0.0 ± 0.0
Gly	3.951GlyAla: 3.951 ± 0.065	0.611GlyCys: 0.611 ± 0.027	3.078GlyAsp: 3.078 ± 0.053	3.232GlyGlu: 3.232 ± 0.05	3.555GlyPhe: 3.555 ± 0.049	4.331GlyGly: 4.331 ± 0.096	0.963GlyHis: 0.963 ± 0.024	5.525GlyIle: 5.525 ± 0.075	5.086GlyLys: 5.086 ± 0.066	5.548GlyLeu: 5.548 ± 0.072	1.455GlyMet: 1.455 ± 0.037	3.878GlyAsn: 3.878 ± 0.062	1.26GlyPro: 1.26 ± 0.041	1.808GlyGln: 1.808 ± 0.038	1.882GlyArg: 1.882 ± 0.04	4.013GlySer: 4.013 ± 0.061	4.206GlyThr: 4.206 ± 0.078	4.216GlyVal: 4.216 ± 0.069	0.684GlyTrp: 0.684 ± 0.023	2.907GlyTyr: 2.907 ± 0.045	0.0GlyXaa: 0.0 ± 0.0
His	0.836HisAla: 0.836 ± 0.024	0.171HisCys: 0.171 ± 0.013	0.781HisAsp: 0.781 ± 0.025	0.928HisGlu: 0.928 ± 0.023	1.087HisPhe: 1.087 ± 0.028	0.935HisGly: 0.935 ± 0.029	0.391HisHis: 0.391 ± 0.016	1.293HisIle: 1.293 ± 0.029	1.098HisLys: 1.098 ± 0.028	1.544HisLeu: 1.544 ± 0.033	0.269HisMet: 0.269 ± 0.014	0.955HisAsn: 0.955 ± 0.024	0.733HisPro: 0.733 ± 0.024	0.587HisGln: 0.587 ± 0.021	0.532HisArg: 0.532 ± 0.019	0.999HisSer: 0.999 ± 0.028	0.863HisThr: 0.863 ± 0.024	0.748HisVal: 0.748 ± 0.026	0.175HisTrp: 0.175 ± 0.011	0.736HisTyr: 0.736 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	5.907IleAla: 5.907 ± 0.072	0.695IleCys: 0.695 ± 0.022	4.966IleAsp: 4.966 ± 0.061	5.682IleGlu: 5.682 ± 0.072	3.811IlePhe: 3.811 ± 0.061	5.25IleGly: 5.25 ± 0.066	1.415IleHis: 1.415 ± 0.038	6.857IleIle: 6.857 ± 0.093	6.639IleLys: 6.639 ± 0.077	7.465IleLeu: 7.465 ± 0.097	1.471IleMet: 1.471 ± 0.034	5.283IleAsn: 5.283 ± 0.063	3.379IlePro: 3.379 ± 0.052	2.728IleGln: 2.728 ± 0.043	2.425IleArg: 2.425 ± 0.04	5.932IleSer: 5.932 ± 0.069	5.471IleThr: 5.471 ± 0.071	5.081IleVal: 5.081 ± 0.063	0.711IleTrp: 0.711 ± 0.021	3.046IleTyr: 3.046 ± 0.054	0.0IleXaa: 0.0 ± 0.0
Lys	5.019LysAla: 5.019 ± 0.072	0.397LysCys: 0.397 ± 0.019	4.649LysAsp: 4.649 ± 0.065	6.286LysGlu: 6.286 ± 0.092	3.179LysPhe: 3.179 ± 0.045	4.633LysGly: 4.633 ± 0.062	1.178LysHis: 1.178 ± 0.027	7.324LysIle: 7.324 ± 0.074	7.51LysLys: 7.51 ± 0.104	6.782LysLeu: 6.782 ± 0.076	2.274LysMet: 2.274 ± 0.04	6.009LysAsn: 6.009 ± 0.08	2.604LysPro: 2.604 ± 0.046	2.72LysGln: 2.72 ± 0.049	2.507LysArg: 2.507 ± 0.04	4.986LysSer: 4.986 ± 0.063	5.296LysThr: 5.296 ± 0.06	4.849LysVal: 4.849 ± 0.059	0.897LysTrp: 0.897 ± 0.027	3.377LysTyr: 3.377 ± 0.056	0.0LysXaa: 0.0 ± 0.0
Leu	5.688LeuAla: 5.688 ± 0.069	0.684LeuCys: 0.684 ± 0.021	4.871LeuAsp: 4.871 ± 0.06	5.576LeuGlu: 5.576 ± 0.07	5.114LeuPhe: 5.114 ± 0.069	5.525LeuGly: 5.525 ± 0.065	1.442LeuHis: 1.442 ± 0.032	7.123LeuIle: 7.123 ± 0.099	7.84LeuLys: 7.84 ± 0.09	8.735LeuLeu: 8.735 ± 0.111	1.961LeuMet: 1.961 ± 0.04	5.884LeuAsn: 5.884 ± 0.072	3.471LeuPro: 3.471 ± 0.049	3.251LeuGln: 3.251 ± 0.056	2.792LeuArg: 2.792 ± 0.054	6.62LeuSer: 6.62 ± 0.082	5.179LeuThr: 5.179 ± 0.067	5.321LeuVal: 5.321 ± 0.056	0.769LeuTrp: 0.769 ± 0.024	3.384LeuTyr: 3.384 ± 0.048	0.0LeuXaa: 0.0 ± 0.0
Met	1.446MetAla: 1.446 ± 0.029	0.148MetCys: 0.148 ± 0.009	1.037MetAsp: 1.037 ± 0.027	1.233MetGlu: 1.233 ± 0.027	0.832MetPhe: 0.832 ± 0.024	1.304MetGly: 1.304 ± 0.028	0.377MetHis: 0.377 ± 0.016	1.612MetIle: 1.612 ± 0.033	2.323MetLys: 2.323 ± 0.041	1.812MetLeu: 1.812 ± 0.04	0.536MetMet: 0.536 ± 0.023	1.348MetAsn: 1.348 ± 0.03	0.808MetPro: 0.808 ± 0.026	0.821MetGln: 0.821 ± 0.027	0.727MetArg: 0.727 ± 0.024	1.414MetSer: 1.414 ± 0.027	1.178MetThr: 1.178 ± 0.031	1.249MetVal: 1.249 ± 0.033	0.14MetTrp: 0.14 ± 0.01	0.704MetTyr: 0.704 ± 0.024	0.0MetXaa: 0.0 ± 0.0
Asn	3.964AsnAla: 3.964 ± 0.057	0.568AsnCys: 0.568 ± 0.025	3.437AsnAsp: 3.437 ± 0.046	4.0AsnGlu: 4.0 ± 0.054	3.271AsnPhe: 3.271 ± 0.05	4.217AsnGly: 4.217 ± 0.079	1.104AsnHis: 1.104 ± 0.027	5.064AsnIle: 5.064 ± 0.067	5.112AsnLys: 5.112 ± 0.071	5.691AsnLeu: 5.691 ± 0.074	1.193AsnMet: 1.193 ± 0.03	4.292AsnAsn: 4.292 ± 0.077	2.911AsnPro: 2.911 ± 0.05	2.46AsnGln: 2.46 ± 0.043	2.063AsnArg: 2.063 ± 0.039	4.309AsnSer: 4.309 ± 0.063	3.943AsnThr: 3.943 ± 0.059	3.605AsnVal: 3.605 ± 0.056	0.851AsnTrp: 0.851 ± 0.026	3.164AsnTyr: 3.164 ± 0.057	0.0AsnXaa: 0.0 ± 0.0
Pro	2.078ProAla: 2.078 ± 0.049	0.218ProCys: 0.218 ± 0.013	1.971ProAsp: 1.971 ± 0.039	2.661ProGlu: 2.661 ± 0.048	1.883ProPhe: 1.883 ± 0.034	1.815ProGly: 1.815 ± 0.045	0.491ProHis: 0.491 ± 0.018	2.821ProIle: 2.821 ± 0.048	2.662ProLys: 2.662 ± 0.043	2.923ProLeu: 2.923 ± 0.048	0.698ProMet: 0.698 ± 0.022	2.344ProAsn: 2.344 ± 0.047	0.763ProPro: 0.763 ± 0.023	1.065ProGln: 1.065 ± 0.029	0.839ProArg: 0.839 ± 0.025	2.142ProSer: 2.142 ± 0.038	2.172ProThr: 2.172 ± 0.051	2.334ProVal: 2.334 ± 0.045	0.286ProTrp: 0.286 ± 0.014	1.402ProTyr: 1.402 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	1.933GlnAla: 1.933 ± 0.043	0.186GlnCys: 0.186 ± 0.012	1.625GlnAsp: 1.625 ± 0.037	2.248GlnGlu: 2.248 ± 0.047	1.677GlnPhe: 1.677 ± 0.032	1.679GlnGly: 1.679 ± 0.036	0.5GlnHis: 0.5 ± 0.019	2.861GlnIle: 2.861 ± 0.041	3.056GlnLys: 3.056 ± 0.048	3.286GlnLeu: 3.286 ± 0.06	0.729GlnMet: 0.729 ± 0.024	2.454GlnAsn: 2.454 ± 0.039	0.964GlnPro: 0.964 ± 0.029	1.289GlnGln: 1.289 ± 0.034	1.065GlnArg: 1.065 ± 0.024	2.093GlnSer: 2.093 ± 0.037	2.01GlnThr: 2.01 ± 0.042	1.852GlnVal: 1.852 ± 0.04	0.363GlnTrp: 0.363 ± 0.017	1.443GlnTyr: 1.443 ± 0.033	0.0GlnXaa: 0.0 ± 0.0
Arg	1.781ArgAla: 1.781 ± 0.035	0.185ArgCys: 0.185 ± 0.01	1.509ArgAsp: 1.509 ± 0.032	1.941ArgGlu: 1.941 ± 0.042	1.705ArgPhe: 1.705 ± 0.041	1.608ArgGly: 1.608 ± 0.032	0.491ArgHis: 0.491 ± 0.016	2.809ArgIle: 2.809 ± 0.046	2.535ArgLys: 2.535 ± 0.046	2.897ArgLeu: 2.897 ± 0.053	0.767ArgMet: 0.767 ± 0.024	2.0ArgAsn: 2.0 ± 0.044	0.937ArgPro: 0.937 ± 0.024	0.892ArgGln: 0.892 ± 0.024	1.037ArgArg: 1.037 ± 0.032	1.669ArgSer: 1.669 ± 0.039	1.642ArgThr: 1.642 ± 0.034	1.874ArgVal: 1.874 ± 0.042	0.356ArgTrp: 0.356 ± 0.013	1.398ArgTyr: 1.398 ± 0.031	0.0ArgXaa: 0.0 ± 0.0
Ser	3.866SerAla: 3.866 ± 0.063	0.712SerCys: 0.712 ± 0.023	3.584SerAsp: 3.584 ± 0.054	3.772SerGlu: 3.772 ± 0.046	4.126SerPhe: 4.126 ± 0.054	4.816SerGly: 4.816 ± 0.076	1.027SerHis: 1.027 ± 0.025	5.678SerIle: 5.678 ± 0.075	5.204SerLys: 5.204 ± 0.064	6.122SerLeu: 6.122 ± 0.081	1.249SerMet: 1.249 ± 0.033	4.085SerAsn: 4.085 ± 0.063	2.16SerPro: 2.16 ± 0.041	2.238SerGln: 2.238 ± 0.042	1.87SerArg: 1.87 ± 0.034	4.478SerSer: 4.478 ± 0.078	3.741SerThr: 3.741 ± 0.061	4.037SerVal: 4.037 ± 0.06	0.721SerTrp: 0.721 ± 0.024	3.023SerTyr: 3.023 ± 0.055	0.0SerXaa: 0.0 ± 0.0
Thr	4.058ThrAla: 4.058 ± 0.074	0.409ThrCys: 0.409 ± 0.022	3.336ThrAsp: 3.336 ± 0.05	3.461ThrGlu: 3.461 ± 0.052	3.15ThrPhe: 3.15 ± 0.043	4.119ThrGly: 4.119 ± 0.078	0.908ThrHis: 0.908 ± 0.026	5.691ThrIle: 5.691 ± 0.066	4.457ThrLys: 4.457 ± 0.056	5.367ThrLeu: 5.367 ± 0.067	0.963ThrMet: 0.963 ± 0.024	3.795ThrAsn: 3.795 ± 0.06	2.638ThrPro: 2.638 ± 0.049	2.027ThrGln: 2.027 ± 0.038	1.595ThrArg: 1.595 ± 0.039	3.903ThrSer: 3.903 ± 0.051	4.073ThrThr: 4.073 ± 0.08	3.979ThrVal: 3.979 ± 0.076	0.571ThrTrp: 0.571 ± 0.023	2.598ThrTyr: 2.598 ± 0.06	0.0ThrXaa: 0.0 ± 0.0
Val	4.063ValAla: 4.063 ± 0.061	0.508ValCys: 0.508 ± 0.02	3.23ValAsp: 3.23 ± 0.049	3.446ValGlu: 3.446 ± 0.049	3.352ValPhe: 3.352 ± 0.051	3.666ValGly: 3.666 ± 0.053	0.893ValHis: 0.893 ± 0.025	5.098ValIle: 5.098 ± 0.059	4.479ValLys: 4.479 ± 0.06	5.821ValLeu: 5.821 ± 0.07	1.255ValMet: 1.255 ± 0.034	3.691ValAsn: 3.691 ± 0.055	2.125ValPro: 2.125 ± 0.044	1.784ValGln: 1.784 ± 0.037	1.782ValArg: 1.782 ± 0.038	4.511ValSer: 4.511 ± 0.062	3.994ValThr: 3.994 ± 0.09	4.113ValVal: 4.113 ± 0.068	0.559ValTrp: 0.559 ± 0.02	2.461ValTyr: 2.461 ± 0.044	0.0ValXaa: 0.0 ± 0.0
Trp	0.593TrpAla: 0.593 ± 0.023	0.1TrpCys: 0.1 ± 0.008	0.64TrpAsp: 0.64 ± 0.021	0.645TrpGlu: 0.645 ± 0.019	0.543TrpPhe: 0.543 ± 0.022	0.672TrpGly: 0.672 ± 0.022	0.211TrpHis: 0.211 ± 0.013	0.807TrpIle: 0.807 ± 0.025	0.785TrpLys: 0.785 ± 0.024	0.899TrpLeu: 0.899 ± 0.028	0.268TrpMet: 0.268 ± 0.012	0.705TrpAsn: 0.705 ± 0.023	0.201TrpPro: 0.201 ± 0.012	0.414TrpGln: 0.414 ± 0.017	0.355TrpArg: 0.355 ± 0.017	0.677TrpSer: 0.677 ± 0.023	0.588TrpThr: 0.588 ± 0.026	0.579TrpVal: 0.579 ± 0.023	0.142TrpTrp: 0.142 ± 0.009	0.439TrpTyr: 0.439 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.455TyrAla: 2.455 ± 0.046	0.352TyrCys: 0.352 ± 0.017	2.536TyrAsp: 2.536 ± 0.046	2.347TyrGlu: 2.347 ± 0.04	2.551TyrPhe: 2.551 ± 0.041	2.55TyrGly: 2.55 ± 0.04	0.718TyrHis: 0.718 ± 0.022	3.073TyrIle: 3.073 ± 0.049	3.416TyrLys: 3.416 ± 0.057	3.853TyrLeu: 3.853 ± 0.054	0.731TyrMet: 0.731 ± 0.022	2.982TyrAsn: 2.982 ± 0.056	1.488TyrPro: 1.488 ± 0.034	1.543TyrGln: 1.543 ± 0.031	1.465TyrArg: 1.465 ± 0.032	3.097TyrSer: 3.097 ± 0.054	2.598TyrThr: 2.598 ± 0.053	2.253TyrVal: 2.253 ± 0.038	0.487TyrTrp: 0.487 ± 0.02	2.135TyrTyr: 2.135 ± 0.053	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4503 proteins (1523331 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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