Cladophialophora immunda
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13606 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D2BWH6|A0A0D2BWH6_9EURO Uncharacterized protein OS=Cladophialophora immunda OX=569365 GN=PV07_11612 PE=4 SV=1
QQ1 pKa = 6.77 QPALAVYY8 pKa = 9.45 QLIQDD13 pKa = 3.94 YY14 pKa = 11.13 SSDD17 pKa = 3.82 AFWQGFDD24 pKa = 4.29 FFTDD28 pKa = 3.74 PDD30 pKa = 3.85 PTDD33 pKa = 3.8 ALVQFQSLEE42 pKa = 3.89 QANATGIAGFIEE54 pKa = 4.58 GGNASFAIYY63 pKa = 9.63 MGVDD67 pKa = 3.55 TEE69 pKa = 4.47 NVAPQGRR76 pKa = 11.84 ASVGVSSSQSFQHH89 pKa = 6.39 ALVIADD95 pKa = 3.73 IVHH98 pKa = 5.95 MPGGVCGSWPAFWMVGANWPNNGEE122 pKa = 3.77 IDD124 pKa = 3.41 IVEE127 pKa = 4.36 GVNDD131 pKa = 3.61 QPTNSMTLHH140 pKa = 6.53 TGQGAVVSNGTDD152 pKa = 3.58 FSGEE156 pKa = 4.64 LITSNCDD163 pKa = 2.9 AGGGVLATEE172 pKa = 4.61 WTADD176 pKa = 4.11 FIKK179 pKa = 10.11 IWFFPRR185 pKa = 11.84 GTFPDD190 pKa = 5.33 DD191 pKa = 3.13 IVSSNPTPSEE201 pKa = 3.51 NWGTRR206 pKa = 11.84 NSLFQGSFNLDD217 pKa = 3.09 DD218 pKa = 4.85 HH219 pKa = 6.99 FNNLQIVFDD228 pKa = 4.02 TTFCGQQ234 pKa = 3.02
Molecular weight: 25.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.704
IPC2_protein 3.656
IPC_protein 3.668
Toseland 3.439
ProMoST 3.846
Dawson 3.668
Bjellqvist 3.834
Wikipedia 3.63
Rodwell 3.49
Grimsley 3.35
Solomon 3.656
Lehninger 3.617
Nozaki 3.795
DTASelect 4.05
Thurlkill 3.516
EMBOSS 3.643
Sillero 3.795
Patrickios 0.947
IPC_peptide 3.656
IPC2_peptide 3.757
IPC2.peptide.svr19 3.709
Protein with the highest isoelectric point:
>tr|A0A0D2CWI6|A0A0D2CWI6_9EURO RNA exonuclease 4 OS=Cladophialophora immunda OX=569365 GN=PV07_01051 PE=3 SV=1
MM1 pKa = 7.12 QYY3 pKa = 10.62 RR4 pKa = 11.84 RR5 pKa = 11.84 IILEE9 pKa = 3.59 QRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 10.28 CSEE16 pKa = 4.18 DD17 pKa = 3.43 KK18 pKa = 10.84 DD19 pKa = 4.19 GKK21 pKa = 7.06 QQRR24 pKa = 11.84 GQEE27 pKa = 4.43 LNPDD31 pKa = 3.45 WAVTGAATRR40 pKa = 11.84 TSLSLKK46 pKa = 10.01 AAVAPRR52 pKa = 11.84 RR53 pKa = 11.84 RR54 pKa = 11.84 SIFSRR59 pKa = 11.84 QGVALVPCRR68 pKa = 11.84 QSLDD72 pKa = 3.36 RR73 pKa = 11.84 RR74 pKa = 11.84 VKK76 pKa = 9.63 LQGIKK81 pKa = 10.44 DD82 pKa = 3.58 HH83 pKa = 6.94 ASPIVRR89 pKa = 11.84 LPYY92 pKa = 10.4 SYY94 pKa = 11.14 AAWTSGFPPLCCRR107 pKa = 11.84 ITHH110 pKa = 6.25 SKK112 pKa = 10.21 HH113 pKa = 5.34 LWLEE117 pKa = 4.01 RR118 pKa = 11.84 SRR120 pKa = 11.84 CVCII124 pKa = 4.63
Molecular weight: 14.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 9.589
IPC_protein 10.394
Toseland 10.687
ProMoST 10.394
Dawson 10.774
Bjellqvist 10.511
Wikipedia 10.994
Rodwell 10.965
Grimsley 10.818
Solomon 10.906
Lehninger 10.877
Nozaki 10.716
DTASelect 10.496
Thurlkill 10.687
EMBOSS 11.096
Sillero 10.716
Patrickios 10.701
IPC_peptide 10.921
IPC2_peptide 9.853
IPC2.peptide.svr19 8.572
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12829
777
13606
6726764
31
6211
494.4
54.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.674 ± 0.018
1.296 ± 0.008
5.691 ± 0.014
6.102 ± 0.018
3.719 ± 0.012
6.768 ± 0.017
2.494 ± 0.009
4.796 ± 0.013
4.622 ± 0.016
9.062 ± 0.02
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.167 ± 0.008
3.512 ± 0.009
6.109 ± 0.019
4.167 ± 0.012
6.328 ± 0.017
8.202 ± 0.021
5.973 ± 0.016
6.106 ± 0.012
1.485 ± 0.008
2.726 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here