Sedimentitalea nanhaiensis
Average proteome isoelectric point is 6.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4762 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I6YJ17|A0A1I6YJ17_9RHOB NTE family protein OS=Sedimentitalea nanhaiensis OX=999627 GN=SAMN05216236_102244 PE=4 SV=1
MM1 pKa = 7.65 SGIGNATRR9 pKa = 11.84 YY10 pKa = 10.05 VSASGDD16 pKa = 3.43 AGIDD20 pKa = 3.88 GILGDD25 pKa = 4.84 RR26 pKa = 11.84 AWDD29 pKa = 3.85 DD30 pKa = 3.33 AALLIGAPPTPDD42 pKa = 3.39 VYY44 pKa = 11.33 SGGYY48 pKa = 10.12 GSGEE52 pKa = 4.21 DD53 pKa = 3.74 SGHH56 pKa = 5.2 VQATAEE62 pKa = 4.34 MTAMMQRR69 pKa = 11.84 SLDD72 pKa = 3.73 RR73 pKa = 11.84 QAGSAADD80 pKa = 4.19 DD81 pKa = 4.25 GFSLEE86 pKa = 4.52 GFTGQTVQQSTAASAHH102 pKa = 5.59 IRR104 pKa = 11.84 IAQTDD109 pKa = 4.26 DD110 pKa = 4.02 DD111 pKa = 4.56 PFGYY115 pKa = 8.14 GTAWAYY121 pKa = 10.53 YY122 pKa = 8.72 PSTGAASGDD131 pKa = 3.43 VWFSTVSYY139 pKa = 10.56 DD140 pKa = 3.15 YY141 pKa = 10.04 TYY143 pKa = 11.03 PVAGNYY149 pKa = 10.41 AYY151 pKa = 9.76 VTMVHH156 pKa = 6.18 EE157 pKa = 5.1 LGHH160 pKa = 6.5 ALGLEE165 pKa = 4.47 HH166 pKa = 6.95 GHH168 pKa = 6.33 LTGSYY173 pKa = 8.25 GTVPTSQDD181 pKa = 2.96 SMEE184 pKa = 4.32 YY185 pKa = 9.72 TVMTYY190 pKa = 10.17 RR191 pKa = 11.84 SYY193 pKa = 11.03 IGASPGNYY201 pKa = 9.77 SNEE204 pKa = 3.74 TWGYY208 pKa = 7.54 AQSWMMLDD216 pKa = 3.66 IAALQHH222 pKa = 5.93 MYY224 pKa = 10.5 GANFTTNSGDD234 pKa = 3.55 TVYY237 pKa = 10.39 KK238 pKa = 9.96 WSPGSGATLVNGGIGIDD255 pKa = 3.49 PGANRR260 pKa = 11.84 IFATVWDD267 pKa = 4.31 GGGTDD272 pKa = 3.75 TFDD275 pKa = 3.4 LSAYY279 pKa = 8.11 ATALDD284 pKa = 3.69 IDD286 pKa = 4.85 LAPGAASVFDD296 pKa = 3.91 SAQLARR302 pKa = 11.84 LGPGEE307 pKa = 3.96 YY308 pKa = 10.43 ARR310 pKa = 11.84 GNIYY314 pKa = 10.54 NALLYY319 pKa = 10.82 QGDD322 pKa = 3.79 TRR324 pKa = 11.84 SLIEE328 pKa = 4.27 HH329 pKa = 6.23 ATGGTGDD336 pKa = 4.55 DD337 pKa = 3.59 RR338 pKa = 11.84 MSGNQADD345 pKa = 3.54 NRR347 pKa = 11.84 LRR349 pKa = 11.84 GGDD352 pKa = 3.8 GNDD355 pKa = 3.28 TLMGQAGNDD364 pKa = 3.37 TLMGQRR370 pKa = 11.84 DD371 pKa = 4.11 DD372 pKa = 5.73 DD373 pKa = 3.82 ILYY376 pKa = 10.97 GNAGNDD382 pKa = 3.59 TLKK385 pKa = 11.31 GNGGNDD391 pKa = 3.26 TLYY394 pKa = 11.41 GNGDD398 pKa = 3.85 DD399 pKa = 5.69 DD400 pKa = 5.72 LLIGHH405 pKa = 7.4 GGHH408 pKa = 7.43 DD409 pKa = 3.5 SLYY412 pKa = 9.99 GQDD415 pKa = 3.83 GNDD418 pKa = 3.49 VLRR421 pKa = 11.84 GIAGQDD427 pKa = 3.29 TLDD430 pKa = 4.32 GGTGDD435 pKa = 5.66 DD436 pKa = 3.59 RR437 pKa = 11.84 LFGNGGADD445 pKa = 3.54 TLSGGAGQDD454 pKa = 3.48 KK455 pKa = 10.88 LFGAAGFDD463 pKa = 3.57 MLYY466 pKa = 11.1 GEE468 pKa = 5.43 AGNDD472 pKa = 3.26 QLTGGRR478 pKa = 11.84 GADD481 pKa = 3.38 QMTGGDD487 pKa = 3.62 GADD490 pKa = 3.05 VFRR493 pKa = 11.84 FVAVNDD499 pKa = 3.94 SPVGGGIDD507 pKa = 3.8 TILDD511 pKa = 3.81 FTPGSDD517 pKa = 4.42 RR518 pKa = 11.84 IDD520 pKa = 3.81 LSGLAATPLGFQGFGNHH537 pKa = 6.17 SGSAPDD543 pKa = 4.51 VILQHH548 pKa = 6.19 VSGDD552 pKa = 3.6 TRR554 pKa = 11.84 ILADD558 pKa = 4.43 LDD560 pKa = 3.8 GDD562 pKa = 3.96 AQSDD566 pKa = 3.88 LRR568 pKa = 11.84 IDD570 pKa = 3.83 LSGVLTLSGADD581 pKa = 3.96 FIFF584 pKa = 4.49
Molecular weight: 60.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.68
IPC2_protein 3.808
IPC_protein 3.872
Toseland 3.63
ProMoST 4.062
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.872
Rodwell 3.694
Grimsley 3.528
Solomon 3.884
Lehninger 3.846
Nozaki 3.999
DTASelect 4.342
Thurlkill 3.694
EMBOSS 3.872
Sillero 3.999
Patrickios 1.252
IPC_peptide 3.884
IPC2_peptide 3.973
IPC2.peptide.svr19 3.877
Protein with the highest isoelectric point:
>tr|A0A1I7CMQ1|A0A1I7CMQ1_9RHOB Diguanylate cyclase/phosphodiesterase OS=Sedimentitalea nanhaiensis OX=999627 GN=SAMN05216236_1183 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 8.96 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.62 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATKK25 pKa = 10.45 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.43 EE41 pKa = 3.72 LSAA44 pKa = 5.03
Molecular weight: 5.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.423
IPC2_protein 10.906
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.092
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4762
0
4762
1450164
28
2361
304.5
33.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.065 ± 0.049
0.938 ± 0.012
6.154 ± 0.031
5.448 ± 0.033
3.713 ± 0.024
8.634 ± 0.039
2.066 ± 0.018
5.26 ± 0.025
3.103 ± 0.03
10.03 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.839 ± 0.017
2.693 ± 0.02
5.104 ± 0.025
3.475 ± 0.021
6.835 ± 0.032
5.249 ± 0.028
5.515 ± 0.022
7.248 ± 0.029
1.385 ± 0.014
2.248 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here