Thermacetogenium phaeum (strain ATCC BAA-254 / DSM 26808 / PB)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Thermoanaerobacterales; Thermoanaerobacteraceae; Thermacetogenium; Thermacetogenium phaeum

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2803 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4LXA5|K4LXA5_THEPS Transposase OS=Thermacetogenium phaeum (strain ATCC BAA-254 / DSM 26808 / PB) OX=1089553 GN=Tph_c24210 PE=4 SV=1
MM1 pKa = 7.7RR2 pKa = 11.84KK3 pKa = 9.19KK4 pKa = 10.69ALVCQVVVLALALMGFGFAKK24 pKa = 9.99WSDD27 pKa = 3.82SVVVRR32 pKa = 11.84ATAQSGSVKK41 pKa = 9.93WGIFDD46 pKa = 3.54EE47 pKa = 5.17SVWQLDD53 pKa = 4.04EE54 pKa = 4.29GTDD57 pKa = 3.11WHH59 pKa = 8.24SEE61 pKa = 4.09MEE63 pKa = 4.43GGMLNYY69 pKa = 10.72DD70 pKa = 3.37QDD72 pKa = 4.25TEE74 pKa = 4.3GKK76 pKa = 9.77DD77 pKa = 3.3VGSTSVEE84 pKa = 3.89VTDD87 pKa = 4.8ANEE90 pKa = 4.75DD91 pKa = 3.85GVKK94 pKa = 9.54DD95 pKa = 3.7TLDD98 pKa = 3.24ITVSNAYY105 pKa = 8.63PCYY108 pKa = 10.03FNEE111 pKa = 3.87ITFDD115 pKa = 3.81VINAGTIPVIVQIPEE130 pKa = 4.21LTWMEE135 pKa = 4.29TEE137 pKa = 4.47SEE139 pKa = 4.31LEE141 pKa = 4.17SGVVYY146 pKa = 7.81YY147 pKa = 10.79LCSNGSIISEE157 pKa = 4.03YY158 pKa = 10.99DD159 pKa = 3.23DD160 pKa = 5.39GFVSPEE166 pKa = 3.62EE167 pKa = 3.92NGAVIEE173 pKa = 5.73VMFQDD178 pKa = 3.52NVSAQQHH185 pKa = 5.72PGEE188 pKa = 4.36VLSEE192 pKa = 4.2SICFHH197 pKa = 5.95VLQPAAQNDD206 pKa = 3.93QYY208 pKa = 11.73SFSLSIEE215 pKa = 3.83GVQWNEE221 pKa = 3.67SPIPAGSVVDD231 pKa = 3.79

Molecular weight: 25.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4LEG3|K4LEG3_THEPS PINc domain-containing protein OS=Thermacetogenium phaeum (strain ATCC BAA-254 / DSM 26808 / PB) OX=1089553 GN=Tph_c01020 PE=4 SV=1
MM1 pKa = 7.21QCGHH5 pKa = 6.92HH6 pKa = 7.3DD7 pKa = 3.29GRR9 pKa = 11.84GPGDD13 pKa = 3.56CLYY16 pKa = 11.13LLRR19 pKa = 11.84HH20 pKa = 5.86QGFEE24 pKa = 4.17GLRR27 pKa = 11.84GTAGEE32 pKa = 3.94QLKK35 pKa = 10.91AGSLHH40 pKa = 6.56RR41 pKa = 11.84ALPRR45 pKa = 11.84PLSGQTPMPPPAAPTEE61 pKa = 4.22YY62 pKa = 10.1EE63 pKa = 3.64NTRR66 pKa = 11.84RR67 pKa = 11.84VCRR70 pKa = 11.84RR71 pKa = 11.84ATYY74 pKa = 9.73GRR76 pKa = 11.84PGNRR80 pKa = 11.84TGEE83 pKa = 3.81ARR85 pKa = 11.84MTGRR89 pKa = 11.84DD90 pKa = 3.37ASTDD94 pKa = 3.42TLRR97 pKa = 11.84VSMEE101 pKa = 4.52WICCEE106 pKa = 4.87AKK108 pKa = 10.43RR109 pKa = 11.84CDD111 pKa = 3.38WQKK114 pKa = 11.07QFATCYY120 pKa = 9.91AASQSCPEE128 pKa = 3.83ASRR131 pKa = 11.84RR132 pKa = 11.84PVRR135 pKa = 11.84PGIGSRR141 pKa = 11.84AVDD144 pKa = 3.6RR145 pKa = 11.84QGMRR149 pKa = 11.84SIFMTGMRR157 pKa = 11.84FPKK160 pKa = 9.94RR161 pKa = 11.84GPGAHH166 pKa = 5.99RR167 pKa = 11.84TPVINLLLQPIGRR180 pKa = 11.84WKK182 pKa = 11.03SNALL186 pKa = 3.44

Molecular weight: 20.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2803

0

2803

812553

29

1762

289.9

32.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.844 ± 0.054

1.41 ± 0.02

4.784 ± 0.033

7.669 ± 0.052

3.786 ± 0.034

8.264 ± 0.045

1.687 ± 0.018

6.185 ± 0.042

5.163 ± 0.047

10.869 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.227 ± 0.019

3.041 ± 0.03

4.591 ± 0.032

3.099 ± 0.025

6.941 ± 0.048

5.014 ± 0.031

4.515 ± 0.027

7.778 ± 0.043

1.058 ± 0.021

3.075 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski