Amino acid dipepetide frequency for Astyanax mexicanus (Blind cave fish) (Astyanax fasciatus mexicanus)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.034AlaAla: 6.034 ± 0.034	1.288AlaCys: 1.288 ± 0.01	3.053AlaAsp: 3.053 ± 0.014	4.685AlaGlu: 4.685 ± 0.021	2.392AlaPhe: 2.392 ± 0.013	4.296AlaGly: 4.296 ± 0.028	1.509AlaHis: 1.509 ± 0.01	2.573AlaIle: 2.573 ± 0.013	3.277AlaLys: 3.277 ± 0.017	6.568AlaLeu: 6.568 ± 0.025	1.469AlaMet: 1.469 ± 0.009	2.136AlaAsn: 2.136 ± 0.012	3.658AlaPro: 3.658 ± 0.025	2.962AlaGln: 2.962 ± 0.021	3.138AlaArg: 3.138 ± 0.019	5.36AlaSer: 5.36 ± 0.023	3.367AlaThr: 3.367 ± 0.019	5.047AlaVal: 5.047 ± 0.02	0.664AlaTrp: 0.664 ± 0.009	1.479AlaTyr: 1.479 ± 0.009	0.0AlaXaa: 0.0 ± 0.0
Cys	1.249CysAla: 1.249 ± 0.011	0.688CysCys: 0.688 ± 0.01	1.059CysAsp: 1.059 ± 0.012	1.304CysGlu: 1.304 ± 0.012	0.915CysPhe: 0.915 ± 0.008	1.678CysGly: 1.678 ± 0.017	0.613CysHis: 0.613 ± 0.007	1.009CysIle: 1.009 ± 0.009	1.186CysLys: 1.186 ± 0.008	2.177CysLeu: 2.177 ± 0.02	0.484CysMet: 0.484 ± 0.005	0.852CysAsn: 0.852 ± 0.009	1.376CysPro: 1.376 ± 0.017	0.98CysGln: 0.98 ± 0.01	1.356CysArg: 1.356 ± 0.013	2.35CysSer: 2.35 ± 0.019	1.249CysThr: 1.249 ± 0.011	1.689CysVal: 1.689 ± 0.016	0.326CysTrp: 0.326 ± 0.005	0.628CysTyr: 0.628 ± 0.006	0.0CysXaa: 0.0 ± 0.0
Asp	2.827AspAla: 2.827 ± 0.013	1.234AspCys: 1.234 ± 0.014	2.905AspAsp: 2.905 ± 0.018	3.623AspGlu: 3.623 ± 0.018	2.083AspPhe: 2.083 ± 0.012	3.483AspGly: 3.483 ± 0.017	1.17AspHis: 1.17 ± 0.008	2.569AspIle: 2.569 ± 0.014	2.561AspLys: 2.561 ± 0.012	5.02AspLeu: 5.02 ± 0.018	1.218AspMet: 1.218 ± 0.007	1.823AspAsn: 1.823 ± 0.012	2.873AspPro: 2.873 ± 0.014	1.987AspGln: 1.987 ± 0.012	2.578AspArg: 2.578 ± 0.017	4.49AspSer: 4.49 ± 0.02	2.578AspThr: 2.578 ± 0.013	3.231AspVal: 3.231 ± 0.016	0.663AspTrp: 0.663 ± 0.007	1.538AspTyr: 1.538 ± 0.01	0.0AspXaa: 0.0 ± 0.0
Glu	4.604GluAla: 4.604 ± 0.023	1.331GluCys: 1.331 ± 0.013	4.332GluAsp: 4.332 ± 0.018	7.65GluGlu: 7.65 ± 0.044	1.97GluPhe: 1.97 ± 0.012	3.999GluGly: 3.999 ± 0.017	1.498GluHis: 1.498 ± 0.008	2.906GluIle: 2.906 ± 0.013	4.959GluLys: 4.959 ± 0.028	6.212GluLeu: 6.212 ± 0.03	1.713GluMet: 1.713 ± 0.012	2.881GluAsn: 2.881 ± 0.015	2.898GluPro: 2.898 ± 0.019	3.156GluGln: 3.156 ± 0.017	4.332GluArg: 4.332 ± 0.026	4.524GluSer: 4.524 ± 0.023	3.429GluThr: 3.429 ± 0.021	4.25GluVal: 4.25 ± 0.018	0.691GluTrp: 0.691 ± 0.007	1.61GluTyr: 1.61 ± 0.01	0.0GluXaa: 0.0 ± 0.0
Phe	1.844PheAla: 1.844 ± 0.011	1.004PheCys: 1.004 ± 0.008	1.685PheAsp: 1.685 ± 0.011	1.855PheGlu: 1.855 ± 0.01	1.759PhePhe: 1.759 ± 0.05	2.16PheGly: 2.16 ± 0.014	0.979PheHis: 0.979 ± 0.007	1.99PheIle: 1.99 ± 0.011	1.8PheLys: 1.8 ± 0.011	3.85PheLeu: 3.85 ± 0.019	0.817PheMet: 0.817 ± 0.007	1.523PheAsn: 1.523 ± 0.011	1.821PhePro: 1.821 ± 0.012	1.633PheGln: 1.633 ± 0.01	1.936PheArg: 1.936 ± 0.011	3.505PheSer: 3.505 ± 0.015	2.417PheThr: 2.417 ± 0.012	2.146PheVal: 2.146 ± 0.011	0.48PheTrp: 0.48 ± 0.005	1.224PheTyr: 1.224 ± 0.008	0.0PheXaa: 0.0 ± 0.0
Gly	3.962GlyAla: 3.962 ± 0.027	1.251GlyCys: 1.251 ± 0.011	3.074GlyAsp: 3.074 ± 0.016	4.056GlyGlu: 4.056 ± 0.021	2.349GlyPhe: 2.349 ± 0.014	4.908GlyGly: 4.908 ± 0.034	1.622GlyHis: 1.622 ± 0.014	2.534GlyIle: 2.534 ± 0.013	3.613GlyLys: 3.613 ± 0.018	5.33GlyLeu: 5.33 ± 0.023	1.429GlyMet: 1.429 ± 0.011	2.401GlyAsn: 2.401 ± 0.012	3.127GlyPro: 3.127 ± 0.028	2.744GlyGln: 2.744 ± 0.021	3.56GlyArg: 3.56 ± 0.017	5.694GlySer: 5.694 ± 0.028	3.457GlyThr: 3.457 ± 0.029	4.012GlyVal: 4.012 ± 0.015	0.778GlyTrp: 0.778 ± 0.009	1.816GlyTyr: 1.816 ± 0.013	0.0GlyXaa: 0.0 ± 0.0
His	1.352HisAla: 1.352 ± 0.009	0.882HisCys: 0.882 ± 0.018	0.932HisAsp: 0.932 ± 0.006	1.207HisGlu: 1.207 ± 0.009	1.059HisPhe: 1.059 ± 0.01	1.513HisGly: 1.513 ± 0.01	1.062HisHis: 1.062 ± 0.015	1.284HisIle: 1.284 ± 0.011	1.279HisLys: 1.279 ± 0.009	2.656HisLeu: 2.656 ± 0.016	0.623HisMet: 0.623 ± 0.006	0.998HisAsn: 0.998 ± 0.008	1.594HisPro: 1.594 ± 0.014	1.344HisGln: 1.344 ± 0.014	1.59HisArg: 1.59 ± 0.01	2.551HisSer: 2.551 ± 0.019	2.023HisThr: 2.023 ± 0.044	1.424HisVal: 1.424 ± 0.009	0.335HisTrp: 0.335 ± 0.004	0.864HisTyr: 0.864 ± 0.007	0.0HisXaa: 0.0 ± 0.0
Ile	2.464IleAla: 2.464 ± 0.012	1.058IleCys: 1.058 ± 0.009	2.054IleAsp: 2.054 ± 0.012	2.418IleGlu: 2.418 ± 0.012	1.862IlePhe: 1.862 ± 0.012	2.232IleGly: 2.232 ± 0.012	1.404IleHis: 1.404 ± 0.02	2.465IleIle: 2.465 ± 0.013	2.588IleLys: 2.588 ± 0.014	4.225IleLeu: 4.225 ± 0.017	1.054IleMet: 1.054 ± 0.008	1.982IleAsn: 1.982 ± 0.011	2.496IlePro: 2.496 ± 0.015	2.165IleGln: 2.165 ± 0.012	2.396IleArg: 2.396 ± 0.012	3.956IleSer: 3.956 ± 0.016	2.895IleThr: 2.895 ± 0.014	2.552IleVal: 2.552 ± 0.015	0.478IleTrp: 0.478 ± 0.005	1.48IleTyr: 1.48 ± 0.019	0.0IleXaa: 0.0 ± 0.0
Lys	3.685LysAla: 3.685 ± 0.017	1.053LysCys: 1.053 ± 0.009	3.158LysAsp: 3.158 ± 0.015	4.749LysGlu: 4.749 ± 0.026	1.611LysPhe: 1.611 ± 0.009	3.132LysGly: 3.132 ± 0.02	1.488LysHis: 1.488 ± 0.011	2.599LysIle: 2.599 ± 0.015	4.908LysLys: 4.908 ± 0.113	5.043LysLeu: 5.043 ± 0.022	1.393LysMet: 1.393 ± 0.009	2.363LysAsn: 2.363 ± 0.015	3.123LysPro: 3.123 ± 0.022	2.592LysGln: 2.592 ± 0.013	3.396LysArg: 3.396 ± 0.017	4.127LysSer: 4.127 ± 0.023	3.239LysThr: 3.239 ± 0.017	3.464LysVal: 3.464 ± 0.016	0.584LysTrp: 0.584 ± 0.005	1.504LysTyr: 1.504 ± 0.011	0.0LysXaa: 0.0 ± 0.0
Leu	5.969LeuAla: 5.969 ± 0.022	2.261LeuCys: 2.261 ± 0.014	4.741LeuAsp: 4.741 ± 0.019	6.395LeuGlu: 6.395 ± 0.03	3.441LeuPhe: 3.441 ± 0.019	5.051LeuGly: 5.051 ± 0.02	2.713LeuHis: 2.713 ± 0.015	3.869LeuIle: 3.869 ± 0.018	5.806LeuLys: 5.806 ± 0.022	9.805LeuLeu: 9.805 ± 0.032	2.052LeuMet: 2.052 ± 0.01	3.779LeuAsn: 3.779 ± 0.015	5.239LeuPro: 5.239 ± 0.023	5.577LeuGln: 5.577 ± 0.028	5.62LeuArg: 5.62 ± 0.03	8.503LeuSer: 8.503 ± 0.035	5.157LeuThr: 5.157 ± 0.021	5.253LeuVal: 5.253 ± 0.018	1.04LeuTrp: 1.04 ± 0.009	2.668LeuTyr: 2.668 ± 0.014	0.0LeuXaa: 0.0 ± 0.0
Met	1.81MetAla: 1.81 ± 0.011	0.5MetCys: 0.5 ± 0.006	1.391MetAsp: 1.391 ± 0.008	1.916MetGlu: 1.916 ± 0.01	0.87MetPhe: 0.87 ± 0.009	1.353MetGly: 1.353 ± 0.009	0.471MetHis: 0.471 ± 0.004	0.89MetIle: 0.89 ± 0.007	1.446MetLys: 1.446 ± 0.009	2.04MetLeu: 2.04 ± 0.012	0.684MetMet: 0.684 ± 0.007	0.914MetAsn: 0.914 ± 0.007	1.11MetPro: 1.11 ± 0.012	0.988MetGln: 0.988 ± 0.008	1.142MetArg: 1.142 ± 0.008	1.802MetSer: 1.802 ± 0.011	1.195MetThr: 1.195 ± 0.008	1.493MetVal: 1.493 ± 0.01	0.251MetTrp: 0.251 ± 0.003	0.639MetTyr: 0.639 ± 0.008	0.0MetXaa: 0.0 ± 0.0
Asn	2.171AsnAla: 2.171 ± 0.012	0.907AsnCys: 0.907 ± 0.009	1.654AsnAsp: 1.654 ± 0.011	2.158AsnGlu: 2.158 ± 0.014	1.418AsnPhe: 1.418 ± 0.01	2.75AsnGly: 2.75 ± 0.015	0.998AsnHis: 0.998 ± 0.007	2.171AsnIle: 2.171 ± 0.012	2.21AsnLys: 2.21 ± 0.012	3.694AsnLeu: 3.694 ± 0.017	1.017AsnMet: 1.017 ± 0.008	1.783AsnAsn: 1.783 ± 0.013	2.246AsnPro: 2.246 ± 0.012	1.766AsnGln: 1.766 ± 0.012	1.992AsnArg: 1.992 ± 0.012	3.387AsnSer: 3.387 ± 0.019	2.343AsnThr: 2.343 ± 0.012	2.321AsnVal: 2.321 ± 0.012	0.461AsnTrp: 0.461 ± 0.005	1.143AsnTyr: 1.143 ± 0.009	0.0AsnXaa: 0.0 ± 0.0
Pro	4.423ProAla: 4.423 ± 0.028	1.083ProCys: 1.083 ± 0.012	2.792ProAsp: 2.792 ± 0.014	3.903ProGlu: 3.903 ± 0.021	1.801ProPhe: 1.801 ± 0.012	3.924ProGly: 3.924 ± 0.03	1.506ProHis: 1.506 ± 0.019	1.907ProIle: 1.907 ± 0.012	2.654ProLys: 2.654 ± 0.018	4.827ProLeu: 4.827 ± 0.021	1.018ProMet: 1.018 ± 0.009	1.928ProAsn: 1.928 ± 0.012	5.493ProPro: 5.493 ± 0.046	2.729ProGln: 2.729 ± 0.02	2.839ProArg: 2.839 ± 0.026	5.58ProSer: 5.58 ± 0.028	3.204ProThr: 3.204 ± 0.023	4.102ProVal: 4.102 ± 0.028	0.54ProTrp: 0.54 ± 0.006	1.525ProTyr: 1.525 ± 0.012	0.0ProXaa: 0.0 ± 0.0
Gln	3.115GlnAla: 3.115 ± 0.019	1.061GlnCys: 1.061 ± 0.012	2.318GlnAsp: 2.318 ± 0.013	3.509GlnGlu: 3.509 ± 0.02	1.385GlnPhe: 1.385 ± 0.011	2.605GlnGly: 2.605 ± 0.02	1.389GlnHis: 1.389 ± 0.011	1.973GlnIle: 1.973 ± 0.011	2.761GlnLys: 2.761 ± 0.016	4.396GlnLeu: 4.396 ± 0.026	1.105GlnMet: 1.105 ± 0.008	1.909GlnAsn: 1.909 ± 0.011	2.632GlnPro: 2.632 ± 0.019	3.342GlnGln: 3.342 ± 0.032	3.136GlnArg: 3.136 ± 0.02	3.694GlnSer: 3.694 ± 0.022	2.613GlnThr: 2.613 ± 0.015	2.703GlnVal: 2.703 ± 0.016	0.548GlnTrp: 0.548 ± 0.005	1.282GlnTyr: 1.282 ± 0.011	0.0GlnXaa: 0.0 ± 0.0
Arg	3.481ArgAla: 3.481 ± 0.019	1.251ArgCys: 1.251 ± 0.016	2.853ArgAsp: 2.853 ± 0.015	3.977ArgGlu: 3.977 ± 0.021	1.96ArgPhe: 1.96 ± 0.01	3.343ArgGly: 3.343 ± 0.021	1.509ArgHis: 1.509 ± 0.018	2.475ArgIle: 2.475 ± 0.013	3.546ArgLys: 3.546 ± 0.016	5.237ArgLeu: 5.237 ± 0.024	1.304ArgMet: 1.304 ± 0.009	2.132ArgAsn: 2.132 ± 0.012	3.078ArgPro: 3.078 ± 0.022	2.557ArgGln: 2.557 ± 0.015	4.341ArgArg: 4.341 ± 0.026	4.654ArgSer: 4.654 ± 0.028	2.968ArgThr: 2.968 ± 0.015	3.372ArgVal: 3.372 ± 0.015	0.663ArgTrp: 0.663 ± 0.007	1.531ArgTyr: 1.531 ± 0.009	0.0ArgXaa: 0.0 ± 0.0
Ser	5.814SerAla: 5.814 ± 0.026	1.969SerCys: 1.969 ± 0.017	4.407SerAsp: 4.407 ± 0.022	5.231SerGlu: 5.231 ± 0.028	3.221SerPhe: 3.221 ± 0.016	5.696SerGly: 5.696 ± 0.026	2.201SerHis: 2.201 ± 0.016	3.48SerIle: 3.48 ± 0.019	4.112SerLys: 4.112 ± 0.02	8.311SerLeu: 8.311 ± 0.037	1.807SerMet: 1.807 ± 0.011	3.06SerAsn: 3.06 ± 0.018	5.983SerPro: 5.983 ± 0.041	3.812SerGln: 3.812 ± 0.02	4.627SerArg: 4.627 ± 0.025	10.559SerSer: 10.559 ± 0.064	5.124SerThr: 5.124 ± 0.024	5.697SerVal: 5.697 ± 0.022	0.995SerTrp: 0.995 ± 0.01	2.188SerTyr: 2.188 ± 0.013	0.0SerXaa: 0.0 ± 0.0
Thr	4.135ThrAla: 4.135 ± 0.021	1.365ThrCys: 1.365 ± 0.015	2.913ThrAsp: 2.913 ± 0.014	3.847ThrGlu: 3.847 ± 0.019	2.068ThrPhe: 2.068 ± 0.012	3.84ThrGly: 3.84 ± 0.026	1.757ThrHis: 1.757 ± 0.044	2.424ThrIle: 2.424 ± 0.014	2.665ThrLys: 2.665 ± 0.014	5.516ThrLeu: 5.516 ± 0.023	1.193ThrMet: 1.193 ± 0.007	1.937ThrAsn: 1.937 ± 0.012	3.724ThrPro: 3.724 ± 0.025	2.462ThrGln: 2.462 ± 0.014	2.462ThrArg: 2.462 ± 0.014	4.779ThrSer: 4.779 ± 0.023	3.465ThrThr: 3.465 ± 0.044	4.367ThrVal: 4.367 ± 0.02	0.644ThrTrp: 0.644 ± 0.007	1.463ThrTyr: 1.463 ± 0.01	0.0ThrXaa: 0.0 ± 0.0
Val	3.928ValAla: 3.928 ± 0.015	1.917ValCys: 1.917 ± 0.018	3.15ValAsp: 3.15 ± 0.013	4.135ValGlu: 4.135 ± 0.018	2.613ValPhe: 2.613 ± 0.014	3.445ValGly: 3.445 ± 0.019	1.575ValHis: 1.575 ± 0.01	2.959ValIle: 2.959 ± 0.014	3.652ValLys: 3.652 ± 0.016	6.318ValLeu: 6.318 ± 0.02	1.506ValMet: 1.506 ± 0.01	2.476ValAsn: 2.476 ± 0.013	3.53ValPro: 3.53 ± 0.021	2.98ValGln: 2.98 ± 0.018	3.329ValArg: 3.329 ± 0.017	5.372ValSer: 5.372 ± 0.024	3.855ValThr: 3.855 ± 0.021	4.257ValVal: 4.257 ± 0.017	0.808ValTrp: 0.808 ± 0.007	1.87ValTyr: 1.87 ± 0.012	0.0ValXaa: 0.0 ± 0.0
Trp	0.668TrpAla: 0.668 ± 0.007	0.274TrpCys: 0.274 ± 0.005	0.621TrpAsp: 0.621 ± 0.006	0.735TrpGlu: 0.735 ± 0.008	0.458TrpPhe: 0.458 ± 0.005	0.637TrpGly: 0.637 ± 0.01	0.266TrpHis: 0.266 ± 0.005	0.599TrpIle: 0.599 ± 0.006	0.71TrpLys: 0.71 ± 0.006	1.095TrpLeu: 1.095 ± 0.008	0.339TrpMet: 0.339 ± 0.004	0.526TrpAsn: 0.526 ± 0.006	0.45TrpPro: 0.45 ± 0.005	0.467TrpGln: 0.467 ± 0.005	0.77TrpArg: 0.77 ± 0.007	0.962TrpSer: 0.962 ± 0.009	0.707TrpThr: 0.707 ± 0.008	0.699TrpVal: 0.699 ± 0.006	0.191TrpTrp: 0.191 ± 0.004	0.362TrpTyr: 0.362 ± 0.005	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.419TyrAla: 1.419 ± 0.009	0.791TyrCys: 0.791 ± 0.007	1.363TyrAsp: 1.363 ± 0.01	1.595TyrGlu: 1.595 ± 0.01	1.194TyrPhe: 1.194 ± 0.009	1.672TyrGly: 1.672 ± 0.012	0.841TyrHis: 0.841 ± 0.008	1.546TyrIle: 1.546 ± 0.02	1.479TyrLys: 1.479 ± 0.009	2.62TyrLeu: 2.62 ± 0.015	0.679TyrMet: 0.679 ± 0.006	1.191TyrAsn: 1.191 ± 0.008	1.326TyrPro: 1.326 ± 0.011	1.233TyrGln: 1.233 ± 0.008	1.693TyrArg: 1.693 ± 0.011	2.442TyrSer: 2.442 ± 0.018	1.739TyrThr: 1.739 ± 0.012	1.595TyrVal: 1.595 ± 0.01	0.396TyrTrp: 0.396 ± 0.005	1.045TyrTyr: 1.045 ± 0.009	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.022XaaXaa: 0.022 ± 0.014

Statistics based on 39383 proteins (22005327 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---