Circoviridae 1 LDMD-2013

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; environmental samples

Average proteome isoelectric point is 8.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5TMT9|S5TMT9_9CIRC Uncharacterized protein OS=Circoviridae 1 LDMD-2013 OX=1379692 PE=4 SV=1
MM1 pKa = 7.06KK2 pKa = 9.69TLTGYY7 pKa = 10.69LSHH10 pKa = 7.05PKK12 pKa = 9.84VRR14 pKa = 11.84GEE16 pKa = 3.86KK17 pKa = 10.22FLVNRR22 pKa = 11.84WIGRR26 pKa = 11.84TFXKK30 pKa = 10.88NKK32 pKa = 6.7MTSIQNARR40 pKa = 11.84VVEE43 pKa = 4.39IYY45 pKa = 11.03VDD47 pKa = 4.03DD48 pKa = 5.21DD49 pKa = 3.78NSEE52 pKa = 4.15YY53 pKa = 10.47EE54 pKa = 3.9QEE56 pKa = 3.96DD57 pKa = 4.48AYY59 pKa = 11.42RR60 pKa = 11.84LATRR64 pKa = 11.84RR65 pKa = 11.84EE66 pKa = 4.09YY67 pKa = 10.3IRR69 pKa = 11.84RR70 pKa = 11.84RR71 pKa = 11.84VEE73 pKa = 3.62DD74 pKa = 3.42RR75 pKa = 11.84NNRR78 pKa = 11.84VARR81 pKa = 11.84RR82 pKa = 11.84LMEE85 pKa = 4.0RR86 pKa = 11.84TVHH89 pKa = 6.81HH90 pKa = 7.07GEE92 pKa = 3.93TDD94 pKa = 3.18MSHH97 pKa = 7.83DD98 pKa = 4.05SDD100 pKa = 3.77TDD102 pKa = 3.53EE103 pKa = 6.0LDD105 pKa = 3.31WEE107 pKa = 4.57LVNN110 pKa = 5.44

Molecular weight: 13.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5TMT9|S5TMT9_9CIRC Uncharacterized protein OS=Circoviridae 1 LDMD-2013 OX=1379692 PE=4 SV=1
MM1 pKa = 7.67SKK3 pKa = 10.22RR4 pKa = 11.84GYY6 pKa = 9.71GQLSHH11 pKa = 7.06APGTYY16 pKa = 10.27DD17 pKa = 3.14EE18 pKa = 5.31ALLGRR23 pKa = 11.84QVKK26 pKa = 10.5RR27 pKa = 11.84IDD29 pKa = 3.19EE30 pKa = 4.49GVRR33 pKa = 11.84VMDD36 pKa = 3.57TQEE39 pKa = 4.01YY40 pKa = 10.08VPLAEE45 pKa = 5.34DD46 pKa = 3.73ADD48 pKa = 4.27EE49 pKa = 4.55VGTGLFDD56 pKa = 3.51LTEE59 pKa = 4.1AASRR63 pKa = 11.84FGPAGLAAGGAAVGGYY79 pKa = 8.32EE80 pKa = 4.4LYY82 pKa = 10.73EE83 pKa = 3.88HH84 pKa = 7.17AAALKK89 pKa = 9.84ADD91 pKa = 3.68AVAAEE96 pKa = 4.64HH97 pKa = 6.02YY98 pKa = 10.04AVKK101 pKa = 10.34KK102 pKa = 8.64VVGMKK107 pKa = 10.07RR108 pKa = 11.84SFMSKK113 pKa = 7.93IHH115 pKa = 5.47EE116 pKa = 4.47AEE118 pKa = 4.06ALAHH122 pKa = 6.49HH123 pKa = 7.08IFHH126 pKa = 7.01EE127 pKa = 4.53SKK129 pKa = 10.59KK130 pKa = 10.08IKK132 pKa = 9.93NVPAAPPRR140 pKa = 11.84PEE142 pKa = 3.77PAFSSKK148 pKa = 10.23RR149 pKa = 11.84GQKK152 pKa = 10.06RR153 pKa = 11.84LLPGGGHH160 pKa = 4.74VVVAGEE166 pKa = 3.71RR167 pKa = 11.84RR168 pKa = 11.84NPKK171 pKa = 9.76KK172 pKa = 10.08HH173 pKa = 6.25FKK175 pKa = 9.84TLDD178 pKa = 3.34SQIQIAQKK186 pKa = 10.41ILNDD190 pKa = 3.65PAASDD195 pKa = 3.41KK196 pKa = 10.84TRR198 pKa = 11.84IYY200 pKa = 8.75YY201 pKa = 7.89TNRR204 pKa = 11.84LQHH207 pKa = 6.46LAAEE211 pKa = 4.32KK212 pKa = 10.76AKK214 pKa = 10.13GKK216 pKa = 10.34VYY218 pKa = 10.16HH219 pKa = 6.63AHH221 pKa = 6.98LKK223 pKa = 9.76PLGGFDD229 pKa = 3.24TTHH232 pKa = 7.56AITAIGYY239 pKa = 7.29VPPDD243 pKa = 3.22EE244 pKa = 4.65RR245 pKa = 11.84FWWSSNGRR253 pKa = 11.84VNTYY257 pKa = 10.16GMHH260 pKa = 6.16GAQRR264 pKa = 11.84RR265 pKa = 11.84AAMKK269 pKa = 10.27KK270 pKa = 7.82GTRR273 pKa = 11.84KK274 pKa = 9.38SAPYY278 pKa = 9.74HH279 pKa = 5.83PPKK282 pKa = 10.52SSARR286 pKa = 11.84YY287 pKa = 8.54RR288 pKa = 11.84GRR290 pKa = 11.84PRR292 pKa = 11.84STPRR296 pKa = 11.84RR297 pKa = 11.84VAKK300 pKa = 9.85PYY302 pKa = 9.99KK303 pKa = 9.44PKK305 pKa = 9.59NHH307 pKa = 6.2ARR309 pKa = 11.84KK310 pKa = 9.55KK311 pKa = 9.56YY312 pKa = 8.9YY313 pKa = 10.31YY314 pKa = 10.54KK315 pKa = 9.34PTQYY319 pKa = 11.63

Molecular weight: 35.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1022

110

358

255.5

28.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.611 ± 1.866

1.272 ± 0.658

4.99 ± 0.875

5.871 ± 1.081

3.425 ± 0.582

8.023 ± 0.976

3.033 ± 0.779

4.501 ± 0.764

7.436 ± 0.928

6.556 ± 0.461

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.957 ± 0.35

3.816 ± 0.978

5.186 ± 1.188

3.327 ± 0.365

8.317 ± 1.128

4.99 ± 0.305

6.164 ± 0.659

6.067 ± 0.546

0.978 ± 0.275

5.186 ± 0.44
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski