Luteovulum ovatum
Taxonomy:
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3594 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A285CU42|A0A285CU42_9RHOB XRE family transcriptional regulator OS=Luteovulum ovatum OX=439529 GN=SAMN05878503_10852 PE=4 SV=1
MM1 pKa = 7.85 RR2 pKa = 11.84 VRR4 pKa = 11.84 VLLVSSALVALSPVVAAAQEE24 pKa = 4.23 YY25 pKa = 10.52 SGGVTLGYY33 pKa = 10.94 GHH35 pKa = 7.21 TDD37 pKa = 2.98 VSDD40 pKa = 3.61 SDD42 pKa = 3.45 TDD44 pKa = 4.31 ANILTLDD51 pKa = 3.33 GRR53 pKa = 11.84 FGVDD57 pKa = 3.24 LGNGLRR63 pKa = 11.84 FGADD67 pKa = 2.58 ISGANVDD74 pKa = 3.6 IDD76 pKa = 4.1 GAADD80 pKa = 4.48 DD81 pKa = 5.26 LDD83 pKa = 3.9 QSVVGIYY90 pKa = 10.0 GAYY93 pKa = 10.01 GFANGTSAGLYY104 pKa = 9.02 VEE106 pKa = 4.97 RR107 pKa = 11.84 ADD109 pKa = 4.77 VDD111 pKa = 4.12 DD112 pKa = 5.87 DD113 pKa = 4.23 LTLTSYY119 pKa = 11.82 GLMLGYY125 pKa = 9.74 EE126 pKa = 4.39 AEE128 pKa = 4.41 GAALGGFYY136 pKa = 10.84 GEE138 pKa = 5.16 SDD140 pKa = 3.8 TDD142 pKa = 3.6 PALPNGGDD150 pKa = 3.49 VQDD153 pKa = 3.97 YY154 pKa = 10.42 GVTFRR159 pKa = 11.84 YY160 pKa = 8.13 TGAEE164 pKa = 4.69 GYY166 pKa = 8.21 TVGASALRR174 pKa = 11.84 TEE176 pKa = 4.27 ISSNGRR182 pKa = 11.84 EE183 pKa = 4.06 LNYY186 pKa = 10.77 DD187 pKa = 3.6 FAGIAGSYY195 pKa = 9.79 AVAPSWTLFGGLSDD209 pKa = 3.73 ISLDD213 pKa = 3.76 EE214 pKa = 4.96 LEE216 pKa = 5.65 LDD218 pKa = 3.85 VTTLGLGVSYY228 pKa = 10.78 DD229 pKa = 3.64 LSAVSRR235 pKa = 11.84 ANASVSLEE243 pKa = 4.03 VARR246 pKa = 11.84 SEE248 pKa = 4.55 TEE250 pKa = 3.87 VSGLEE255 pKa = 3.84 GDD257 pKa = 3.81 EE258 pKa = 4.18 TSVRR262 pKa = 11.84 LGLSIPLGSRR272 pKa = 11.84 GFAVPMNSVADD283 pKa = 3.69 SVLNPRR289 pKa = 11.84 HH290 pKa = 5.85 SAMSTSMMSAYY301 pKa = 10.34
Molecular weight: 30.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.687
IPC2_protein 3.77
IPC_protein 3.783
Toseland 3.567
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.719
Rodwell 3.605
Grimsley 3.465
Solomon 3.77
Lehninger 3.732
Nozaki 3.897
DTASelect 4.151
Thurlkill 3.617
EMBOSS 3.732
Sillero 3.91
Patrickios 1.189
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.799
Protein with the highest isoelectric point:
>tr|A0A285CPH0|A0A285CPH0_9RHOB Transcriptional regulator /LysR family transcriptional regulator OS=Luteovulum ovatum OX=439529 GN=SAMN05878503_10450 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.39 GGRR28 pKa = 11.84 LVLNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.06 GRR39 pKa = 11.84 KK40 pKa = 8.83 KK41 pKa = 10.63 LSAA44 pKa = 3.95
Molecular weight: 5.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3594
0
3594
1137852
26
12455
316.6
34.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.791 ± 0.087
0.883 ± 0.02
5.833 ± 0.036
5.511 ± 0.037
3.499 ± 0.029
8.923 ± 0.049
2.018 ± 0.024
4.678 ± 0.029
2.578 ± 0.038
10.515 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.549 ± 0.03
2.118 ± 0.022
5.631 ± 0.054
2.993 ± 0.021
7.782 ± 0.049
4.702 ± 0.037
5.373 ± 0.058
7.338 ± 0.043
1.419 ± 0.018
1.867 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here