Rhodobacteraceae bacterium

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; unclassified Rhodobacteraceae

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3201 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K9JPW8|A0A2K9JPW8_9RHOB Phosphatidylglycerophosphatase A OS=Rhodobacteraceae bacterium OX=1904441 GN=B9057_06915 PE=4 SV=1
MM1 pKa = 7.27KK2 pKa = 7.86TTLTHH7 pKa = 5.45VAGLCFVATGAIAEE21 pKa = 4.41TSTLTIYY28 pKa = 10.36TYY30 pKa = 11.26DD31 pKa = 3.56GFNSEE36 pKa = 4.84WGPGPQIEE44 pKa = 4.74ANFEE48 pKa = 4.13AEE50 pKa = 4.49CACDD54 pKa = 3.93LEE56 pKa = 5.12FVAAGDD62 pKa = 3.84GAAVLSRR69 pKa = 11.84LKK71 pKa = 11.15LEE73 pKa = 4.68GANTDD78 pKa = 3.14ADD80 pKa = 3.72IVLGLDD86 pKa = 3.41TNLTADD92 pKa = 3.72AAATGLFAASGVTAAYY108 pKa = 8.98DD109 pKa = 4.75LPIEE113 pKa = 4.23WSDD116 pKa = 3.47DD117 pKa = 3.49TFVPFDD123 pKa = 3.01WGYY126 pKa = 9.49FAFVAGKK133 pKa = 10.21DD134 pKa = 3.14VDD136 pKa = 3.61APTNFRR142 pKa = 11.84EE143 pKa = 4.78LAASDD148 pKa = 3.98LKK150 pKa = 10.93IVIQDD155 pKa = 3.85PRR157 pKa = 11.84SSTPGLGLVLWVEE170 pKa = 4.06AAYY173 pKa = 10.44GDD175 pKa = 3.85EE176 pKa = 5.24AGSIWADD183 pKa = 3.18LADD186 pKa = 4.59NIVTVTPGWSEE197 pKa = 4.06AYY199 pKa = 10.34GLFLEE204 pKa = 5.29GEE206 pKa = 4.16ADD208 pKa = 3.5AVLSYY213 pKa = 7.6TTSPAYY219 pKa = 10.26HH220 pKa = 7.29LIAEE224 pKa = 4.75GDD226 pKa = 3.7DD227 pKa = 4.73SKK229 pKa = 10.33TSWAFEE235 pKa = 4.11EE236 pKa = 4.21GHH238 pKa = 5.52YY239 pKa = 9.6MQVEE243 pKa = 4.47VAAKK247 pKa = 9.85VASTDD252 pKa = 3.53VPEE255 pKa = 4.64LADD258 pKa = 3.54AFLAFMTTEE267 pKa = 5.09GFQSVIPTTNWMYY280 pKa = 10.47PAVTPAVGLPEE291 pKa = 4.41GFEE294 pKa = 4.33TLVTPSTALLLSSEE308 pKa = 4.16DD309 pKa = 3.53AAAKK313 pKa = 10.12SGPAIDD319 pKa = 3.62AWRR322 pKa = 11.84AALAQQ327 pKa = 3.73

Molecular weight: 34.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K9JJ31|A0A2K9JJ31_9RHOB UvrABC system protein B OS=Rhodobacteraceae bacterium OX=1904441 GN=uvrB PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84VRR12 pKa = 11.84KK13 pKa = 8.99NRR15 pKa = 11.84HH16 pKa = 3.77GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.97VLSAA44 pKa = 4.11

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3201

0

3201

992233

41

2628

310.0

33.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.611 ± 0.053

0.886 ± 0.013

6.322 ± 0.043

5.909 ± 0.038

3.813 ± 0.025

8.413 ± 0.042

2.007 ± 0.023

5.672 ± 0.03

3.569 ± 0.034

9.625 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.916 ± 0.023

2.943 ± 0.029

4.689 ± 0.033

3.15 ± 0.023

6.203 ± 0.048

5.328 ± 0.033

5.763 ± 0.038

7.483 ± 0.038

1.355 ± 0.019

2.342 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski