Amino acid dipepetide frequency for Rhodobacteraceae bacterium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	14.735AlaAla: 14.735 ± 0.193	0.976AlaCys: 0.976 ± 0.036	7.005AlaAsp: 7.005 ± 0.097	7.919AlaGlu: 7.919 ± 0.121	4.141AlaPhe: 4.141 ± 0.071	9.638AlaGly: 9.638 ± 0.112	2.062AlaHis: 2.062 ± 0.043	6.442AlaIle: 6.442 ± 0.086	4.395AlaLys: 4.395 ± 0.083	12.123AlaLeu: 12.123 ± 0.128	3.777AlaMet: 3.777 ± 0.071	3.161AlaAsn: 3.161 ± 0.065	4.9AlaPro: 4.9 ± 0.078	4.071AlaGln: 4.071 ± 0.066	7.037AlaArg: 7.037 ± 0.097	5.574AlaSer: 5.574 ± 0.081	6.212AlaThr: 6.212 ± 0.093	8.103AlaVal: 8.103 ± 0.101	1.301AlaTrp: 1.301 ± 0.041	2.541AlaTyr: 2.541 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	1.006CysAla: 1.006 ± 0.036	0.103CysCys: 0.103 ± 0.01	0.652CysAsp: 0.652 ± 0.025	0.463CysGlu: 0.463 ± 0.02	0.326CysPhe: 0.326 ± 0.019	0.956CysGly: 0.956 ± 0.032	0.281CysHis: 0.281 ± 0.018	0.464CysIle: 0.464 ± 0.023	0.234CysLys: 0.234 ± 0.016	0.754CysLeu: 0.754 ± 0.026	0.192CysMet: 0.192 ± 0.013	0.249CysAsn: 0.249 ± 0.016	0.512CysPro: 0.512 ± 0.023	0.236CysGln: 0.236 ± 0.016	0.532CysArg: 0.532 ± 0.024	0.491CysSer: 0.491 ± 0.022	0.431CysThr: 0.431 ± 0.026	0.623CysVal: 0.623 ± 0.026	0.11CysTrp: 0.11 ± 0.012	0.242CysTyr: 0.242 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	7.272AspAla: 7.272 ± 0.102	0.587AspCys: 0.587 ± 0.023	3.896AspAsp: 3.896 ± 0.116	3.735AspGlu: 3.735 ± 0.066	2.475AspPhe: 2.475 ± 0.05	5.696AspGly: 5.696 ± 0.103	1.513AspHis: 1.513 ± 0.045	3.422AspIle: 3.422 ± 0.06	1.911AspLys: 1.911 ± 0.042	6.638AspLeu: 6.638 ± 0.092	1.827AspMet: 1.827 ± 0.052	1.509AspAsn: 1.509 ± 0.047	3.415AspPro: 3.415 ± 0.067	2.122AspGln: 2.122 ± 0.049	4.344AspArg: 4.344 ± 0.073	2.152AspSer: 2.152 ± 0.055	3.281AspThr: 3.281 ± 0.092	4.562AspVal: 4.562 ± 0.078	1.104AspTrp: 1.104 ± 0.033	1.757AspTyr: 1.757 ± 0.041	0.0AspXaa: 0.0 ± 0.0
Glu	7.863GluAla: 7.863 ± 0.12	0.378GluCys: 0.378 ± 0.021	3.426GluAsp: 3.426 ± 0.072	3.545GluGlu: 3.545 ± 0.08	2.001GluPhe: 2.001 ± 0.043	4.694GluGly: 4.694 ± 0.074	1.154GluHis: 1.154 ± 0.034	3.682GluIle: 3.682 ± 0.065	2.409GluLys: 2.409 ± 0.058	5.203GluLeu: 5.203 ± 0.074	1.875GluMet: 1.875 ± 0.043	1.917GluAsn: 1.917 ± 0.046	2.317GluPro: 2.317 ± 0.061	2.016GluGln: 2.016 ± 0.054	3.995GluArg: 3.995 ± 0.074	2.197GluSer: 2.197 ± 0.046	3.899GluThr: 3.899 ± 0.068	4.653GluVal: 4.653 ± 0.068	0.773GluTrp: 0.773 ± 0.035	1.099GluTyr: 1.099 ± 0.038	0.0GluXaa: 0.0 ± 0.0
Phe	4.425PheAla: 4.425 ± 0.074	0.419PheCys: 0.419 ± 0.022	3.052PheAsp: 3.052 ± 0.049	2.301PheGlu: 2.301 ± 0.048	1.498PhePhe: 1.498 ± 0.049	3.918PheGly: 3.918 ± 0.069	0.747PheHis: 0.747 ± 0.031	1.89PheIle: 1.89 ± 0.044	1.149PheLys: 1.149 ± 0.035	3.227PheLeu: 3.227 ± 0.08	0.944PheMet: 0.944 ± 0.033	1.242PheAsn: 1.242 ± 0.041	1.49PhePro: 1.49 ± 0.041	0.999PheGln: 0.999 ± 0.031	2.024PheArg: 2.024 ± 0.054	2.091PheSer: 2.091 ± 0.055	2.22PheThr: 2.22 ± 0.057	2.886PheVal: 2.886 ± 0.069	0.59PheTrp: 0.59 ± 0.027	1.023PheTyr: 1.023 ± 0.032	0.0PheXaa: 0.0 ± 0.0
Gly	8.982GlyAla: 8.982 ± 0.113	0.887GlyCys: 0.887 ± 0.032	4.873GlyAsp: 4.873 ± 0.11	4.621GlyGlu: 4.621 ± 0.079	3.695GlyPhe: 3.695 ± 0.068	6.94GlyGly: 6.94 ± 0.119	1.885GlyHis: 1.885 ± 0.045	4.756GlyIle: 4.756 ± 0.079	3.429GlyLys: 3.429 ± 0.076	8.564GlyLeu: 8.564 ± 0.115	2.6GlyMet: 2.6 ± 0.05	2.329GlyAsn: 2.329 ± 0.078	3.238GlyPro: 3.238 ± 0.057	3.084GlyGln: 3.084 ± 0.059	5.157GlyArg: 5.157 ± 0.08	4.284GlySer: 4.284 ± 0.073	4.583GlyThr: 4.583 ± 0.076	6.505GlyVal: 6.505 ± 0.073	1.343GlyTrp: 1.343 ± 0.038	2.376GlyTyr: 2.376 ± 0.046	0.0GlyXaa: 0.0 ± 0.0
His	2.006HisAla: 2.006 ± 0.041	0.235HisCys: 0.235 ± 0.015	1.315HisAsp: 1.315 ± 0.041	1.031HisGlu: 1.031 ± 0.031	0.83HisPhe: 0.83 ± 0.026	1.87HisGly: 1.87 ± 0.045	0.609HisHis: 0.609 ± 0.037	1.063HisIle: 1.063 ± 0.035	0.594HisLys: 0.594 ± 0.029	2.037HisLeu: 2.037 ± 0.05	0.601HisMet: 0.601 ± 0.028	0.535HisAsn: 0.535 ± 0.022	1.227HisPro: 1.227 ± 0.037	0.594HisGln: 0.594 ± 0.023	1.267HisArg: 1.267 ± 0.036	0.994HisSer: 0.994 ± 0.03	0.907HisThr: 0.907 ± 0.034	1.44HisVal: 1.44 ± 0.041	0.352HisTrp: 0.352 ± 0.02	0.562HisTyr: 0.562 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	7.422IleAla: 7.422 ± 0.078	0.644IleCys: 0.644 ± 0.026	4.114IleAsp: 4.114 ± 0.068	3.944IleGlu: 3.944 ± 0.061	1.877IlePhe: 1.877 ± 0.048	5.216IleGly: 5.216 ± 0.083	0.975IleHis: 0.975 ± 0.032	2.713IleIle: 2.713 ± 0.057	1.78IleLys: 1.78 ± 0.047	5.172IleLeu: 5.172 ± 0.085	1.277IleMet: 1.277 ± 0.036	1.657IleAsn: 1.657 ± 0.055	2.566IlePro: 2.566 ± 0.05	1.357IleGln: 1.357 ± 0.037	3.289IleArg: 3.289 ± 0.061	3.18IleSer: 3.18 ± 0.058	3.192IleThr: 3.192 ± 0.058	4.339IleVal: 4.339 ± 0.073	0.721IleTrp: 0.721 ± 0.029	1.293IleTyr: 1.293 ± 0.038	0.0IleXaa: 0.0 ± 0.0
Lys	4.251LysAla: 4.251 ± 0.076	0.225LysCys: 0.225 ± 0.015	2.082LysAsp: 2.082 ± 0.052	1.855LysGlu: 1.855 ± 0.049	1.233LysPhe: 1.233 ± 0.041	3.012LysGly: 3.012 ± 0.062	0.675LysHis: 0.675 ± 0.027	1.974LysIle: 1.974 ± 0.05	1.431LysLys: 1.431 ± 0.055	3.268LysLeu: 3.268 ± 0.066	1.043LysMet: 1.043 ± 0.039	0.968LysAsn: 0.968 ± 0.033	1.883LysPro: 1.883 ± 0.051	1.062LysGln: 1.062 ± 0.035	2.397LysArg: 2.397 ± 0.053	2.208LysSer: 2.208 ± 0.048	2.186LysThr: 2.186 ± 0.051	2.714LysVal: 2.714 ± 0.063	0.464LysTrp: 0.464 ± 0.021	0.756LysTyr: 0.756 ± 0.031	0.0LysXaa: 0.0 ± 0.0
Leu	11.217LeuAla: 11.217 ± 0.133	0.927LeuCys: 0.927 ± 0.033	5.959LeuAsp: 5.959 ± 0.082	5.045LeuGlu: 5.045 ± 0.084	3.526LeuPhe: 3.526 ± 0.078	7.831LeuGly: 7.831 ± 0.109	1.745LeuHis: 1.745 ± 0.044	5.609LeuIle: 5.609 ± 0.095	3.459LeuLys: 3.459 ± 0.062	8.073LeuLeu: 8.073 ± 0.113	2.824LeuMet: 2.824 ± 0.053	3.022LeuAsn: 3.022 ± 0.052	5.097LeuPro: 5.097 ± 0.07	2.689LeuGln: 2.689 ± 0.049	6.397LeuArg: 6.397 ± 0.086	6.89LeuSer: 6.89 ± 0.093	6.147LeuThr: 6.147 ± 0.088	6.464LeuVal: 6.464 ± 0.094	1.297LeuTrp: 1.297 ± 0.043	2.038LeuTyr: 2.038 ± 0.049	0.0LeuXaa: 0.0 ± 0.0
Met	3.455MetAla: 3.455 ± 0.064	0.203MetCys: 0.203 ± 0.013	1.575MetAsp: 1.575 ± 0.042	1.47MetGlu: 1.47 ± 0.04	0.9MetPhe: 0.9 ± 0.03	2.399MetGly: 2.399 ± 0.058	0.478MetHis: 0.478 ± 0.026	1.8MetIle: 1.8 ± 0.044	1.211MetLys: 1.211 ± 0.039	2.554MetLeu: 2.554 ± 0.053	0.877MetMet: 0.877 ± 0.036	0.991MetAsn: 0.991 ± 0.031	1.486MetPro: 1.486 ± 0.038	0.952MetGln: 0.952 ± 0.033	1.92MetArg: 1.92 ± 0.044	1.84MetSer: 1.84 ± 0.041	2.335MetThr: 2.335 ± 0.052	2.018MetVal: 2.018 ± 0.044	0.303MetTrp: 0.303 ± 0.017	0.394MetTyr: 0.394 ± 0.022	0.0MetXaa: 0.0 ± 0.0
Asn	3.447AsnAla: 3.447 ± 0.059	0.299AsnCys: 0.299 ± 0.015	1.891AsnAsp: 1.891 ± 0.071	1.37AsnGlu: 1.37 ± 0.041	1.085AsnPhe: 1.085 ± 0.035	2.982AsnGly: 2.982 ± 0.072	0.6AsnHis: 0.6 ± 0.026	1.563AsnIle: 1.563 ± 0.038	0.874AsnLys: 0.874 ± 0.032	2.76AsnLeu: 2.76 ± 0.065	0.829AsnMet: 0.829 ± 0.035	0.792AsnAsn: 0.792 ± 0.03	1.95AsnPro: 1.95 ± 0.048	0.775AsnGln: 0.775 ± 0.028	1.927AsnArg: 1.927 ± 0.05	1.309AsnSer: 1.309 ± 0.037	1.586AsnThr: 1.586 ± 0.044	2.163AsnVal: 2.163 ± 0.051	0.48AsnTrp: 0.48 ± 0.022	0.751AsnTyr: 0.751 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	4.953ProAla: 4.953 ± 0.085	0.351ProCys: 0.351 ± 0.021	3.606ProAsp: 3.606 ± 0.069	3.778ProGlu: 3.778 ± 0.077	1.941ProPhe: 1.941 ± 0.05	2.903ProGly: 2.903 ± 0.05	0.972ProHis: 0.972 ± 0.032	2.618ProIle: 2.618 ± 0.05	1.835ProLys: 1.835 ± 0.051	4.32ProLeu: 4.32 ± 0.07	1.332ProMet: 1.332 ± 0.034	1.577ProAsn: 1.577 ± 0.039	1.707ProPro: 1.707 ± 0.044	1.529ProGln: 1.529 ± 0.042	2.395ProArg: 2.395 ± 0.055	2.675ProSer: 2.675 ± 0.054	2.719ProThr: 2.719 ± 0.058	3.992ProVal: 3.992 ± 0.066	0.581ProTrp: 0.581 ± 0.026	1.106ProTyr: 1.106 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	3.609GlnAla: 3.609 ± 0.067	0.213GlnCys: 0.213 ± 0.013	1.623GlnAsp: 1.623 ± 0.044	1.494GlnGlu: 1.494 ± 0.037	1.19GlnPhe: 1.19 ± 0.036	2.395GlnGly: 2.395 ± 0.046	0.567GlnHis: 0.567 ± 0.021	2.209GlnIle: 2.209 ± 0.046	1.195GlnLys: 1.195 ± 0.038	2.92GlnLeu: 2.92 ± 0.057	1.156GlnMet: 1.156 ± 0.035	1.008GlnAsn: 1.008 ± 0.028	1.439GlnPro: 1.439 ± 0.04	0.972GlnGln: 0.972 ± 0.034	2.058GlnArg: 2.058 ± 0.049	2.05GlnSer: 2.05 ± 0.05	1.98GlnThr: 1.98 ± 0.043	2.371GlnVal: 2.371 ± 0.045	0.439GlnTrp: 0.439 ± 0.021	0.614GlnTyr: 0.614 ± 0.025	0.0GlnXaa: 0.0 ± 0.0
Arg	6.802ArgAla: 6.802 ± 0.101	0.454ArgCys: 0.454 ± 0.023	4.148ArgAsp: 4.148 ± 0.067	3.599ArgGlu: 3.599 ± 0.069	2.478ArgPhe: 2.478 ± 0.054	4.255ArgGly: 4.255 ± 0.075	1.368ArgHis: 1.368 ± 0.039	3.696ArgIle: 3.696 ± 0.066	2.432ArgLys: 2.432 ± 0.057	6.606ArgLeu: 6.606 ± 0.1	1.917ArgMet: 1.917 ± 0.044	1.856ArgAsn: 1.856 ± 0.042	2.873ArgPro: 2.873 ± 0.05	2.25ArgGln: 2.25 ± 0.054	4.265ArgArg: 4.265 ± 0.083	3.3ArgSer: 3.3 ± 0.059	2.959ArgThr: 2.959 ± 0.064	4.423ArgVal: 4.423 ± 0.079	0.843ArgTrp: 0.843 ± 0.034	1.506ArgTyr: 1.506 ± 0.041	0.0ArgXaa: 0.0 ± 0.0
Ser	5.799SerAla: 5.799 ± 0.088	0.466SerCys: 0.466 ± 0.023	3.511SerAsp: 3.511 ± 0.068	2.951SerGlu: 2.951 ± 0.059	2.298SerPhe: 2.298 ± 0.049	5.303SerGly: 5.303 ± 0.08	1.103SerHis: 1.103 ± 0.036	2.962SerIle: 2.962 ± 0.056	1.805SerLys: 1.805 ± 0.043	5.077SerLeu: 5.077 ± 0.073	1.521SerMet: 1.521 ± 0.038	1.607SerAsn: 1.607 ± 0.037	2.403SerPro: 2.403 ± 0.053	1.639SerGln: 1.639 ± 0.039	3.083SerArg: 3.083 ± 0.062	2.727SerSer: 2.727 ± 0.06	2.771SerThr: 2.771 ± 0.059	4.171SerVal: 4.171 ± 0.064	0.667SerTrp: 0.667 ± 0.027	1.415SerTyr: 1.415 ± 0.038	0.0SerXaa: 0.0 ± 0.0
Thr	6.449ThrAla: 6.449 ± 0.095	0.461ThrCys: 0.461 ± 0.023	3.502ThrAsp: 3.502 ± 0.068	3.292ThrGlu: 3.292 ± 0.058	2.197ThrPhe: 2.197 ± 0.055	5.247ThrGly: 5.247 ± 0.085	1.128ThrHis: 1.128 ± 0.04	3.306ThrIle: 3.306 ± 0.072	1.802ThrLys: 1.802 ± 0.044	5.977ThrLeu: 5.977 ± 0.085	1.358ThrMet: 1.358 ± 0.034	1.602ThrAsn: 1.602 ± 0.041	3.236ThrPro: 3.236 ± 0.056	1.688ThrGln: 1.688 ± 0.045	3.308ThrArg: 3.308 ± 0.064	2.935ThrSer: 2.935 ± 0.058	3.219ThrThr: 3.219 ± 0.068	4.788ThrVal: 4.788 ± 0.094	0.676ThrTrp: 0.676 ± 0.025	1.461ThrTyr: 1.461 ± 0.041	0.0ThrXaa: 0.0 ± 0.0
Val	8.578ValAla: 8.578 ± 0.106	0.639ValCys: 0.639 ± 0.023	4.454ValAsp: 4.454 ± 0.067	4.714ValGlu: 4.714 ± 0.072	2.863ValPhe: 2.863 ± 0.055	5.665ValGly: 5.665 ± 0.094	1.341ValHis: 1.341 ± 0.038	4.705ValIle: 4.705 ± 0.069	2.585ValLys: 2.585 ± 0.055	7.161ValLeu: 7.161 ± 0.104	2.246ValMet: 2.246 ± 0.054	2.243ValAsn: 2.243 ± 0.059	3.496ValPro: 3.496 ± 0.056	2.198ValGln: 2.198 ± 0.046	4.141ValArg: 4.141 ± 0.068	4.315ValSer: 4.315 ± 0.071	5.039ValThr: 5.039 ± 0.091	5.91ValVal: 5.91 ± 0.106	0.937ValTrp: 0.937 ± 0.032	1.595ValTyr: 1.595 ± 0.038	0.0ValXaa: 0.0 ± 0.0
Trp	1.313TrpAla: 1.313 ± 0.042	0.129TrpCys: 0.129 ± 0.012	0.799TrpAsp: 0.799 ± 0.027	0.633TrpGlu: 0.633 ± 0.032	0.589TrpPhe: 0.589 ± 0.028	0.995TrpGly: 0.995 ± 0.033	0.328TrpHis: 0.328 ± 0.021	0.758TrpIle: 0.758 ± 0.03	0.505TrpLys: 0.505 ± 0.026	1.524TrpLeu: 1.524 ± 0.044	0.41TrpMet: 0.41 ± 0.02	0.454TrpAsn: 0.454 ± 0.021	0.602TrpPro: 0.602 ± 0.021	0.509TrpGln: 0.509 ± 0.023	0.959TrpArg: 0.959 ± 0.031	0.789TrpSer: 0.789 ± 0.032	0.779TrpThr: 0.779 ± 0.032	1.004TrpVal: 1.004 ± 0.034	0.207TrpTrp: 0.207 ± 0.014	0.269TrpTyr: 0.269 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.529TyrAla: 2.529 ± 0.06	0.263TyrCys: 0.263 ± 0.017	1.733TyrAsp: 1.733 ± 0.046	1.336TyrGlu: 1.336 ± 0.041	0.992TyrPhe: 0.992 ± 0.029	2.215TyrGly: 2.215 ± 0.052	0.539TyrHis: 0.539 ± 0.023	1.093TyrIle: 1.093 ± 0.032	0.653TyrLys: 0.653 ± 0.025	2.297TyrLeu: 2.297 ± 0.056	0.552TyrMet: 0.552 ± 0.023	0.715TyrAsn: 0.715 ± 0.028	1.054TyrPro: 1.054 ± 0.032	0.761TyrGln: 0.761 ± 0.029	1.538TyrArg: 1.538 ± 0.041	1.279TyrSer: 1.279 ± 0.037	1.185TyrThr: 1.185 ± 0.046	1.695TyrVal: 1.695 ± 0.04	0.367TyrTrp: 0.367 ± 0.02	0.623TyrTyr: 0.623 ± 0.026	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3201 proteins (992233 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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