Gordonia phage Ruthy

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Ruthyvirus; Gordonia virus Ruthy

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345L5G0|A0A345L5G0_9CAUD Uncharacterized protein OS=Gordonia phage Ruthy OX=2250323 GN=49 PE=4 SV=1
MM1 pKa = 7.09NTFLDD6 pKa = 4.39IILVRR11 pKa = 11.84TAPTPTSFIFAMCFWGALTVCVASYY36 pKa = 10.69LPRR39 pKa = 11.84LGGWLTDD46 pKa = 3.41ADD48 pKa = 5.34LDD50 pKa = 5.18DD51 pKa = 5.78EE52 pKa = 5.0YY53 pKa = 11.72DD54 pKa = 4.32DD55 pKa = 5.79DD56 pKa = 6.71LEE58 pKa = 4.32FTPIDD63 pKa = 3.76HH64 pKa = 6.2GHH66 pKa = 5.92IRR68 pKa = 3.56

Molecular weight: 7.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345L5I3|A0A345L5I3_9CAUD HNH endonuclease OS=Gordonia phage Ruthy OX=2250323 GN=72 PE=4 SV=1
MM1 pKa = 7.5TGRR4 pKa = 11.84NTDD7 pKa = 3.41HH8 pKa = 6.92LHH10 pKa = 4.98ITHH13 pKa = 7.47AEE15 pKa = 3.75RR16 pKa = 11.84DD17 pKa = 3.65KK18 pKa = 11.47LDD20 pKa = 3.91RR21 pKa = 11.84EE22 pKa = 4.37LVALIDD28 pKa = 3.74WLAEE32 pKa = 3.85ALTDD36 pKa = 3.88TLTQQTRR43 pKa = 11.84TGSGPRR49 pKa = 11.84VTTGRR54 pKa = 11.84GKK56 pKa = 9.75EE57 pKa = 3.79QPLPYY62 pKa = 9.83NSRR65 pKa = 11.84AAKK68 pKa = 8.65VARR71 pKa = 11.84RR72 pKa = 11.84LRR74 pKa = 11.84DD75 pKa = 3.43NLNRR79 pKa = 11.84AIIDD83 pKa = 3.65TCHH86 pKa = 5.83QRR88 pKa = 11.84HH89 pKa = 6.05LPHH92 pKa = 7.25PGHH95 pKa = 5.52TTRR98 pKa = 11.84ITSSTPVPYY107 pKa = 10.44APQFRR112 pKa = 11.84TFNATRR118 pKa = 11.84LLSIHH123 pKa = 7.43DD124 pKa = 3.71AATWLHH130 pKa = 6.29HH131 pKa = 6.01PRR133 pKa = 11.84HH134 pKa = 6.3LIALTLTDD142 pKa = 3.79TATAHH147 pKa = 5.97YY148 pKa = 9.95SAILAAITAAHH159 pKa = 6.15NAIEE163 pKa = 4.38RR164 pKa = 11.84PRR166 pKa = 11.84EE167 pKa = 3.59PDD169 pKa = 3.32YY170 pKa = 11.6VGSCTRR176 pKa = 11.84CNGDD180 pKa = 2.8LWGFDD185 pKa = 3.67DD186 pKa = 6.42DD187 pKa = 4.65KK188 pKa = 11.43TITCGACGLTIQRR201 pKa = 11.84IDD203 pKa = 2.84QDD205 pKa = 3.32ARR207 pKa = 11.84IDD209 pKa = 3.58RR210 pKa = 11.84EE211 pKa = 3.84LRR213 pKa = 11.84ARR215 pKa = 11.84LFTARR220 pKa = 11.84EE221 pKa = 4.01LVTIVADD228 pKa = 5.0RR229 pKa = 11.84LGQTIKK235 pKa = 10.74PKK237 pKa = 9.63TVYY240 pKa = 10.74ALTYY244 pKa = 10.31RR245 pKa = 11.84RR246 pKa = 11.84GNPITVRR253 pKa = 11.84GTNRR257 pKa = 11.84SGDD260 pKa = 3.65NLYY263 pKa = 11.0LCGDD267 pKa = 3.72VLDD270 pKa = 4.58ALTRR274 pKa = 11.84RR275 pKa = 11.84PRR277 pKa = 11.84RR278 pKa = 11.84RR279 pKa = 11.84KK280 pKa = 8.36QHH282 pKa = 5.6AVSAASRR289 pKa = 11.84HH290 pKa = 4.6TAQQ293 pKa = 4.34

Molecular weight: 32.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

16447

61

1653

225.3

24.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.3 ± 0.329

0.991 ± 0.172

6.98 ± 0.286

5.655 ± 0.243

2.779 ± 0.175

8.299 ± 0.46

2.341 ± 0.23

4.7 ± 0.203

3.454 ± 0.319

7.606 ± 0.223

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.97 ± 0.122

2.718 ± 0.19

5.855 ± 0.216

3.496 ± 0.158

7.327 ± 0.448

5.466 ± 0.21

6.883 ± 0.29

7.175 ± 0.213

1.958 ± 0.128

2.049 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski