Amino acid dipepetide frequency for Gordonia phage Ruthy

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.694AlaAla: 17.694 ± 1.529
1.216AlaCys: 1.216 ± 0.344
8.574AlaAsp: 8.574 ± 0.713
8.513AlaGlu: 8.513 ± 0.701
3.527AlaPhe: 3.527 ± 0.578
9.729AlaGly: 9.729 ± 1.325
2.007AlaHis: 2.007 ± 0.408
6.81AlaIle: 6.81 ± 0.471
4.682AlaLys: 4.682 ± 0.989
8.756AlaLeu: 8.756 ± 0.877
3.101AlaMet: 3.101 ± 0.398
3.344AlaAsn: 3.344 ± 0.425
7.114AlaPro: 7.114 ± 0.845
4.621AlaGln: 4.621 ± 0.571
8.269AlaArg: 8.269 ± 1.17
5.716AlaSer: 5.716 ± 0.774
6.202AlaThr: 6.202 ± 0.654
8.574AlaVal: 8.574 ± 0.747
2.189AlaTrp: 2.189 ± 0.401
2.371AlaTyr: 2.371 ± 0.362
0.0AlaXaa: 0.0 ± 0.0
Cys
0.973CysAla: 0.973 ± 0.254
0.426CysCys: 0.426 ± 0.215
0.73CysAsp: 0.73 ± 0.246
0.851CysGlu: 0.851 ± 0.248
0.061CysPhe: 0.061 ± 0.062
1.277CysGly: 1.277 ± 0.323
0.365CysHis: 0.365 ± 0.201
0.122CysIle: 0.122 ± 0.081
0.122CysLys: 0.122 ± 0.105
0.365CysLeu: 0.365 ± 0.182
0.122CysMet: 0.122 ± 0.096
0.304CysAsn: 0.304 ± 0.12
0.547CysPro: 0.547 ± 0.231
0.182CysGln: 0.182 ± 0.097
1.034CysArg: 1.034 ± 0.272
0.79CysSer: 0.79 ± 0.238
0.73CysThr: 0.73 ± 0.223
0.547CysVal: 0.547 ± 0.181
0.122CysTrp: 0.122 ± 0.077
0.243CysTyr: 0.243 ± 0.119
0.0CysXaa: 0.0 ± 0.0
Asp
8.513AspAla: 8.513 ± 0.757
0.486AspCys: 0.486 ± 0.176
6.141AspAsp: 6.141 ± 0.756
4.256AspGlu: 4.256 ± 0.509
1.277AspPhe: 1.277 ± 0.278
6.263AspGly: 6.263 ± 0.602
2.675AspHis: 2.675 ± 0.395
2.615AspIle: 2.615 ± 0.327
1.946AspLys: 1.946 ± 0.324
5.716AspLeu: 5.716 ± 0.714
1.277AspMet: 1.277 ± 0.263
1.885AspAsn: 1.885 ± 0.399
5.472AspPro: 5.472 ± 0.909
2.615AspGln: 2.615 ± 0.409
5.108AspArg: 5.108 ± 0.722
2.554AspSer: 2.554 ± 0.484
4.256AspThr: 4.256 ± 0.428
3.831AspVal: 3.831 ± 0.437
1.581AspTrp: 1.581 ± 0.284
1.277AspTyr: 1.277 ± 0.293
0.0AspXaa: 0.0 ± 0.0
Glu
6.141GluAla: 6.141 ± 0.705
0.669GluCys: 0.669 ± 0.26
3.223GluAsp: 3.223 ± 0.516
2.311GluGlu: 2.311 ± 0.493
2.493GluPhe: 2.493 ± 0.421
3.831GluGly: 3.831 ± 0.547
1.338GluHis: 1.338 ± 0.322
2.432GluIle: 2.432 ± 0.4
2.311GluLys: 2.311 ± 0.521
6.141GluLeu: 6.141 ± 0.718
1.399GluMet: 1.399 ± 0.301
1.642GluAsn: 1.642 ± 0.307
2.797GluPro: 2.797 ± 0.422
2.675GluGln: 2.675 ± 0.307
4.196GluArg: 4.196 ± 0.648
3.04GluSer: 3.04 ± 0.418
3.587GluThr: 3.587 ± 0.461
3.892GluVal: 3.892 ± 0.557
1.034GluTrp: 1.034 ± 0.216
1.399GluTyr: 1.399 ± 0.23
0.0GluXaa: 0.0 ± 0.0
Phe
4.013PheAla: 4.013 ± 0.628
0.122PheCys: 0.122 ± 0.083
1.459PheAsp: 1.459 ± 0.285
1.581PheGlu: 1.581 ± 0.236
0.304PhePhe: 0.304 ± 0.125
2.736PheGly: 2.736 ± 0.528
0.912PheHis: 0.912 ± 0.255
1.034PheIle: 1.034 ± 0.287
1.094PheLys: 1.094 ± 0.286
1.52PheLeu: 1.52 ± 0.409
0.669PheMet: 0.669 ± 0.192
1.034PheAsn: 1.034 ± 0.216
1.642PhePro: 1.642 ± 0.368
0.669PheGln: 0.669 ± 0.221
1.885PheArg: 1.885 ± 0.334
1.581PheSer: 1.581 ± 0.293
2.493PheThr: 2.493 ± 0.402
1.763PheVal: 1.763 ± 0.332
0.73PheTrp: 0.73 ± 0.226
0.547PheTyr: 0.547 ± 0.186
0.0PheXaa: 0.0 ± 0.0
Gly
8.695GlyAla: 8.695 ± 0.853
0.669GlyCys: 0.669 ± 0.266
6.506GlyAsp: 6.506 ± 0.664
4.56GlyGlu: 4.56 ± 0.523
2.979GlyPhe: 2.979 ± 0.571
6.081GlyGly: 6.081 ± 0.818
1.642GlyHis: 1.642 ± 0.293
4.378GlyIle: 4.378 ± 1.165
2.919GlyLys: 2.919 ± 0.388
7.54GlyLeu: 7.54 ± 0.828
1.763GlyMet: 1.763 ± 0.302
2.128GlyAsn: 2.128 ± 0.318
4.013GlyPro: 4.013 ± 0.639
3.04GlyGln: 3.04 ± 0.509
5.776GlyArg: 5.776 ± 0.597
5.533GlySer: 5.533 ± 0.732
4.439GlyThr: 4.439 ± 0.487
6.628GlyVal: 6.628 ± 0.629
1.581GlyTrp: 1.581 ± 0.34
2.128GlyTyr: 2.128 ± 0.341
0.0GlyXaa: 0.0 ± 0.0
His
2.615HisAla: 2.615 ± 0.422
0.122HisCys: 0.122 ± 0.089
1.824HisAsp: 1.824 ± 0.473
0.912HisGlu: 0.912 ± 0.289
0.669HisPhe: 0.669 ± 0.201
1.763HisGly: 1.763 ± 0.362
0.973HisHis: 0.973 ± 0.328
1.155HisIle: 1.155 ± 0.278
0.547HisLys: 0.547 ± 0.169
2.067HisLeu: 2.067 ± 0.314
0.243HisMet: 0.243 ± 0.124
0.486HisAsn: 0.486 ± 0.139
1.885HisPro: 1.885 ± 0.358
1.034HisGln: 1.034 ± 0.286
1.338HisArg: 1.338 ± 0.336
1.216HisSer: 1.216 ± 0.278
1.885HisThr: 1.885 ± 0.342
1.216HisVal: 1.216 ± 0.214
0.73HisTrp: 0.73 ± 0.176
0.73HisTyr: 0.73 ± 0.205
0.0HisXaa: 0.0 ± 0.0
Ile
6.993IleAla: 6.993 ± 0.78
0.365IleCys: 0.365 ± 0.205
4.256IleAsp: 4.256 ± 0.486
3.527IleGlu: 3.527 ± 0.384
1.338IlePhe: 1.338 ± 0.323
4.013IleGly: 4.013 ± 0.726
1.034IleHis: 1.034 ± 0.252
1.824IleIle: 1.824 ± 0.384
2.007IleLys: 2.007 ± 0.476
2.432IleLeu: 2.432 ± 0.372
0.547IleMet: 0.547 ± 0.174
0.912IleAsn: 0.912 ± 0.298
2.919IlePro: 2.919 ± 0.538
1.277IleGln: 1.277 ± 0.325
2.919IleArg: 2.919 ± 0.332
2.493IleSer: 2.493 ± 0.401
3.587IleThr: 3.587 ± 0.501
3.04IleVal: 3.04 ± 0.353
0.608IleTrp: 0.608 ± 0.205
0.912IleTyr: 0.912 ± 0.241
0.0IleXaa: 0.0 ± 0.0
Lys
4.56LysAla: 4.56 ± 0.918
0.122LysCys: 0.122 ± 0.092
2.007LysAsp: 2.007 ± 0.327
1.824LysGlu: 1.824 ± 0.318
0.851LysPhe: 0.851 ± 0.298
2.25LysGly: 2.25 ± 0.398
0.547LysHis: 0.547 ± 0.169
1.946LysIle: 1.946 ± 0.443
1.277LysLys: 1.277 ± 0.354
2.736LysLeu: 2.736 ± 0.432
0.73LysMet: 0.73 ± 0.215
1.277LysAsn: 1.277 ± 0.627
1.885LysPro: 1.885 ± 0.347
1.094LysGln: 1.094 ± 0.267
2.615LysArg: 2.615 ± 0.353
1.703LysSer: 1.703 ± 0.342
2.858LysThr: 2.858 ± 0.43
3.223LysVal: 3.223 ± 0.457
0.426LysTrp: 0.426 ± 0.179
0.608LysTyr: 0.608 ± 0.197
0.0LysXaa: 0.0 ± 0.0
Leu
9.607LeuAla: 9.607 ± 0.739
0.486LeuCys: 0.486 ± 0.172
5.959LeuAsp: 5.959 ± 0.596
3.77LeuGlu: 3.77 ± 0.456
2.371LeuPhe: 2.371 ± 0.35
6.263LeuGly: 6.263 ± 0.548
1.642LeuHis: 1.642 ± 0.3
4.013LeuIle: 4.013 ± 0.528
1.763LeuLys: 1.763 ± 0.239
5.716LeuLeu: 5.716 ± 0.598
1.459LeuMet: 1.459 ± 0.38
2.128LeuAsn: 2.128 ± 0.421
3.831LeuPro: 3.831 ± 0.468
2.25LeuGln: 2.25 ± 0.427
6.689LeuArg: 6.689 ± 0.691
4.621LeuSer: 4.621 ± 0.588
5.776LeuThr: 5.776 ± 0.594
4.864LeuVal: 4.864 ± 0.616
1.703LeuTrp: 1.703 ± 0.417
1.155LeuTyr: 1.155 ± 0.27
0.0LeuXaa: 0.0 ± 0.0
Met
3.04MetAla: 3.04 ± 0.396
0.486MetCys: 0.486 ± 0.189
0.669MetAsp: 0.669 ± 0.197
0.547MetGlu: 0.547 ± 0.259
0.547MetPhe: 0.547 ± 0.168
1.459MetGly: 1.459 ± 0.305
0.365MetHis: 0.365 ± 0.181
0.79MetIle: 0.79 ± 0.206
0.608MetLys: 0.608 ± 0.182
1.155MetLeu: 1.155 ± 0.34
0.426MetMet: 0.426 ± 0.203
0.486MetAsn: 0.486 ± 0.205
1.216MetPro: 1.216 ± 0.268
0.912MetGln: 0.912 ± 0.189
0.912MetArg: 0.912 ± 0.247
1.338MetSer: 1.338 ± 0.313
2.432MetThr: 2.432 ± 0.379
1.52MetVal: 1.52 ± 0.264
0.426MetTrp: 0.426 ± 0.154
0.365MetTyr: 0.365 ± 0.142
0.0MetXaa: 0.0 ± 0.0
Asn
3.162AsnAla: 3.162 ± 0.466
0.243AsnCys: 0.243 ± 0.118
1.338AsnAsp: 1.338 ± 0.31
1.642AsnGlu: 1.642 ± 0.282
0.669AsnPhe: 0.669 ± 0.209
2.797AsnGly: 2.797 ± 0.438
0.973AsnHis: 0.973 ± 0.277
0.73AsnIle: 0.73 ± 0.203
0.73AsnLys: 0.73 ± 0.269
2.128AsnLeu: 2.128 ± 0.436
0.426AsnMet: 0.426 ± 0.152
0.486AsnAsn: 0.486 ± 0.171
2.493AsnPro: 2.493 ± 0.458
0.851AsnGln: 0.851 ± 0.195
2.493AsnArg: 2.493 ± 0.438
1.399AsnSer: 1.399 ± 0.259
1.581AsnThr: 1.581 ± 0.3
1.946AsnVal: 1.946 ± 0.5
0.669AsnTrp: 0.669 ± 0.272
0.426AsnTyr: 0.426 ± 0.187
0.0AsnXaa: 0.0 ± 0.0
Pro
7.418ProAla: 7.418 ± 0.769
0.73ProCys: 0.73 ± 0.212
4.804ProAsp: 4.804 ± 0.55
3.527ProGlu: 3.527 ± 0.456
0.912ProPhe: 0.912 ± 0.232
5.472ProGly: 5.472 ± 0.655
0.973ProHis: 0.973 ± 0.267
2.554ProIle: 2.554 ± 0.433
2.371ProLys: 2.371 ± 0.322
3.04ProLeu: 3.04 ± 0.412
1.216ProMet: 1.216 ± 0.267
1.094ProAsn: 1.094 ± 0.265
3.344ProPro: 3.344 ± 0.517
2.615ProGln: 2.615 ± 0.43
3.466ProArg: 3.466 ± 0.556
3.587ProSer: 3.587 ± 0.592
5.29ProThr: 5.29 ± 0.594
3.831ProVal: 3.831 ± 0.388
1.034ProTrp: 1.034 ± 0.303
1.277ProTyr: 1.277 ± 0.337
0.0ProXaa: 0.0 ± 0.0
Gln
4.804GlnAla: 4.804 ± 0.456
0.486GlnCys: 0.486 ± 0.183
1.277GlnAsp: 1.277 ± 0.271
0.851GlnGlu: 0.851 ± 0.339
1.399GlnPhe: 1.399 ± 0.293
2.615GlnGly: 2.615 ± 0.384
1.034GlnHis: 1.034 ± 0.278
1.459GlnIle: 1.459 ± 0.29
1.399GlnLys: 1.399 ± 0.376
3.77GlnLeu: 3.77 ± 0.478
0.547GlnMet: 0.547 ± 0.18
1.094GlnAsn: 1.094 ± 0.289
1.946GlnPro: 1.946 ± 0.313
1.52GlnGln: 1.52 ± 0.319
3.162GlnArg: 3.162 ± 0.484
1.763GlnSer: 1.763 ± 0.373
1.763GlnThr: 1.763 ± 0.322
2.371GlnVal: 2.371 ± 0.309
0.973GlnTrp: 0.973 ± 0.349
0.73GlnTyr: 0.73 ± 0.232
0.0GlnXaa: 0.0 ± 0.0
Arg
8.269ArgAla: 8.269 ± 0.797
0.973ArgCys: 0.973 ± 0.231
4.925ArgAsp: 4.925 ± 0.593
4.5ArgGlu: 4.5 ± 0.627
1.824ArgPhe: 1.824 ± 0.362
5.168ArgGly: 5.168 ± 0.561
2.007ArgHis: 2.007 ± 0.461
3.892ArgIle: 3.892 ± 0.487
2.493ArgLys: 2.493 ± 0.379
5.472ArgLeu: 5.472 ± 0.609
1.642ArgMet: 1.642 ± 0.301
2.128ArgAsn: 2.128 ± 0.285
3.892ArgPro: 3.892 ± 0.574
2.736ArgGln: 2.736 ± 0.465
6.932ArgArg: 6.932 ± 1.125
3.04ArgSer: 3.04 ± 0.441
4.5ArgThr: 4.5 ± 0.629
5.472ArgVal: 5.472 ± 0.694
1.824ArgTrp: 1.824 ± 0.357
1.581ArgTyr: 1.581 ± 0.368
0.0ArgXaa: 0.0 ± 0.0
Ser
6.81SerAla: 6.81 ± 0.733
0.304SerCys: 0.304 ± 0.12
2.797SerAsp: 2.797 ± 0.384
2.858SerGlu: 2.858 ± 0.346
1.338SerPhe: 1.338 ± 0.315
6.749SerGly: 6.749 ± 0.862
1.399SerHis: 1.399 ± 0.273
2.675SerIle: 2.675 ± 0.366
1.703SerLys: 1.703 ± 0.264
3.344SerLeu: 3.344 ± 0.466
1.094SerMet: 1.094 ± 0.341
1.459SerAsn: 1.459 ± 0.252
2.979SerPro: 2.979 ± 0.454
1.642SerGln: 1.642 ± 0.327
3.831SerArg: 3.831 ± 0.652
3.223SerSer: 3.223 ± 0.447
4.013SerThr: 4.013 ± 0.453
4.56SerVal: 4.56 ± 0.438
0.669SerTrp: 0.669 ± 0.164
1.216SerTyr: 1.216 ± 0.322
0.0SerXaa: 0.0 ± 0.0
Thr
6.81ThrAla: 6.81 ± 0.58
0.79ThrCys: 0.79 ± 0.273
5.412ThrAsp: 5.412 ± 0.572
3.892ThrGlu: 3.892 ± 0.558
2.311ThrPhe: 2.311 ± 0.348
5.898ThrGly: 5.898 ± 0.664
1.459ThrHis: 1.459 ± 0.368
3.77ThrIle: 3.77 ± 0.428
2.615ThrLys: 2.615 ± 0.545
4.804ThrLeu: 4.804 ± 0.563
1.034ThrMet: 1.034 ± 0.237
1.824ThrAsn: 1.824 ± 0.386
3.77ThrPro: 3.77 ± 0.435
1.459ThrGln: 1.459 ± 0.244
5.047ThrArg: 5.047 ± 0.89
3.344ThrSer: 3.344 ± 0.492
5.655ThrThr: 5.655 ± 0.624
6.081ThrVal: 6.081 ± 0.575
1.034ThrTrp: 1.034 ± 0.26
1.824ThrTyr: 1.824 ± 0.433
0.0ThrXaa: 0.0 ± 0.0
Val
8.148ValAla: 8.148 ± 0.628
0.73ValCys: 0.73 ± 0.218
5.108ValAsp: 5.108 ± 0.626
4.986ValGlu: 4.986 ± 0.707
1.824ValPhe: 1.824 ± 0.272
4.986ValGly: 4.986 ± 0.606
0.851ValHis: 0.851 ± 0.198
3.162ValIle: 3.162 ± 0.433
2.615ValLys: 2.615 ± 0.336
6.081ValLeu: 6.081 ± 0.522
1.399ValMet: 1.399 ± 0.255
2.067ValAsn: 2.067 ± 0.395
4.743ValPro: 4.743 ± 0.399
2.554ValGln: 2.554 ± 0.379
4.621ValArg: 4.621 ± 0.51
4.682ValSer: 4.682 ± 0.678
4.925ValThr: 4.925 ± 0.529
6.081ValVal: 6.081 ± 0.643
1.459ValTrp: 1.459 ± 0.381
0.73ValTyr: 0.73 ± 0.222
0.0ValXaa: 0.0 ± 0.0
Trp
1.581TrpAla: 1.581 ± 0.331
0.304TrpCys: 0.304 ± 0.148
1.338TrpAsp: 1.338 ± 0.282
0.912TrpGlu: 0.912 ± 0.236
0.486TrpPhe: 0.486 ± 0.163
1.52TrpGly: 1.52 ± 0.372
0.73TrpHis: 0.73 ± 0.238
0.851TrpIle: 0.851 ± 0.272
0.669TrpLys: 0.669 ± 0.176
2.128TrpLeu: 2.128 ± 0.317
0.486TrpMet: 0.486 ± 0.165
0.79TrpAsn: 0.79 ± 0.319
0.912TrpPro: 0.912 ± 0.225
0.73TrpGln: 0.73 ± 0.23
1.277TrpArg: 1.277 ± 0.273
1.52TrpSer: 1.52 ± 0.333
1.459TrpThr: 1.459 ± 0.263
1.034TrpVal: 1.034 ± 0.281
0.243TrpTrp: 0.243 ± 0.128
0.608TrpTyr: 0.608 ± 0.257
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.162TyrAla: 3.162 ± 0.466
0.182TyrCys: 0.182 ± 0.108
1.459TyrAsp: 1.459 ± 0.304
0.73TyrGlu: 0.73 ± 0.252
0.608TyrPhe: 0.608 ± 0.203
2.128TyrGly: 2.128 ± 0.285
0.486TyrHis: 0.486 ± 0.184
0.79TyrIle: 0.79 ± 0.241
0.669TyrLys: 0.669 ± 0.186
1.155TyrLeu: 1.155 ± 0.239
0.061TyrMet: 0.061 ± 0.06
0.608TyrAsn: 0.608 ± 0.206
1.155TyrPro: 1.155 ± 0.281
0.486TyrGln: 0.486 ± 0.159
1.703TyrArg: 1.703 ± 0.361
1.52TyrSer: 1.52 ± 0.28
1.399TyrThr: 1.399 ± 0.38
1.277TyrVal: 1.277 ± 0.367
0.547TyrTrp: 0.547 ± 0.166
0.365TyrTyr: 0.365 ± 0.176
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 73 proteins (16447 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski