Bos taurus papillomavirus 8
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A6XA98|A6XA98_BPV5 Major capsid protein L1 OS=Bos taurus papillomavirus 8 OX=2758968 GN=L1 PE=3 SV=1
MM1 pKa = 7.47 AVRR4 pKa = 11.84 VRR6 pKa = 11.84 RR7 pKa = 11.84 VKK9 pKa = 10.41 RR10 pKa = 11.84 ANPYY14 pKa = 10.57 DD15 pKa = 4.17 LYY17 pKa = 9.98 RR18 pKa = 11.84 TCATGDD24 pKa = 3.64 CPQDD28 pKa = 3.34 VKK30 pKa = 11.59 DD31 pKa = 3.92 KK32 pKa = 11.21 FEE34 pKa = 5.49 HH35 pKa = 5.53 NTLADD40 pKa = 5.16 KK41 pKa = 10.46 ILKK44 pKa = 8.89 WGSTGIFLGGLGIGTGQGRR63 pKa = 11.84 PGLGTYY69 pKa = 10.32 SPLGRR74 pKa = 11.84 GGGGGVSGRR83 pKa = 11.84 IPTRR87 pKa = 11.84 PSGSRR92 pKa = 11.84 PLGRR96 pKa = 11.84 PFNSGPIDD104 pKa = 3.66 TIGAGGLRR112 pKa = 11.84 PSVEE116 pKa = 4.13 SSVNSADD123 pKa = 3.89 VVALLPDD130 pKa = 3.87 SPSVVTPDD138 pKa = 4.25 SMPVDD143 pKa = 4.08 PGVGGLDD150 pKa = 2.89 IHH152 pKa = 8.29 AEE154 pKa = 4.23 VVEE157 pKa = 4.48 EE158 pKa = 3.9 PSLIFVEE165 pKa = 4.68 PHH167 pKa = 4.84 ATEE170 pKa = 4.44 DD171 pKa = 4.2 VAVLDD176 pKa = 4.33 MNPAEE181 pKa = 4.84 HH182 pKa = 6.91 DD183 pKa = 3.54 QSVHH187 pKa = 5.76 LSSSTTHH194 pKa = 6.5 HH195 pKa = 6.09 NPSFQGHH202 pKa = 4.34 VTLYY206 pKa = 9.87 TDD208 pKa = 2.87 IGEE211 pKa = 4.33 TSEE214 pKa = 5.79 VEE216 pKa = 4.11 NLWIGGTSIGSNRR229 pKa = 11.84 GEE231 pKa = 4.35 EE232 pKa = 3.77 IQMQLFSTPRR242 pKa = 11.84 SSTPEE247 pKa = 3.5 TEE249 pKa = 4.28 AIGRR253 pKa = 11.84 VRR255 pKa = 11.84 GRR257 pKa = 11.84 ANWFSRR263 pKa = 11.84 RR264 pKa = 11.84 YY265 pKa = 6.48 YY266 pKa = 8.3 TQVSVRR272 pKa = 11.84 DD273 pKa = 3.97 PKK275 pKa = 10.89 FLEE278 pKa = 3.87 QPRR281 pKa = 11.84 EE282 pKa = 3.74 YY283 pKa = 9.8 FTYY286 pKa = 10.28 GFEE289 pKa = 4.25 NPAYY293 pKa = 9.83 EE294 pKa = 4.71 PDD296 pKa = 3.84 PLEE299 pKa = 4.92 DD300 pKa = 3.58 SFDD303 pKa = 3.69 VSEE306 pKa = 4.4 VSPSVRR312 pKa = 11.84 VEE314 pKa = 3.68 PEE316 pKa = 3.63 FRR318 pKa = 11.84 DD319 pKa = 3.78 LTHH322 pKa = 5.21 VTAAKK327 pKa = 9.47 TLTGNTGRR335 pKa = 11.84 LGVSRR340 pKa = 11.84 VGTKK344 pKa = 10.57 LSIGTRR350 pKa = 11.84 SGVRR354 pKa = 11.84 IGGQVHH360 pKa = 5.95 FRR362 pKa = 11.84 YY363 pKa = 10.12 SLSTIADD370 pKa = 3.69 EE371 pKa = 5.18 EE372 pKa = 4.39 GLEE375 pKa = 3.95 LLPRR379 pKa = 11.84 SAAEE383 pKa = 4.26 GSAGPLQHH391 pKa = 6.8 SAEE394 pKa = 4.31 TVLTDD399 pKa = 3.06 GHH401 pKa = 6.29 DD402 pKa = 3.5 AYY404 pKa = 11.7 VEE406 pKa = 3.98 VDD408 pKa = 3.42 HH409 pKa = 7.01 NSTGSAYY416 pKa = 10.78 SEE418 pKa = 3.86 IDD420 pKa = 3.66 LLDD423 pKa = 3.99 EE424 pKa = 5.91 DD425 pKa = 4.92 EE426 pKa = 4.47 VTPHH430 pKa = 5.98 GVLVFHH436 pKa = 7.25 DD437 pKa = 5.01 RR438 pKa = 11.84 PAEE441 pKa = 3.93 TDD443 pKa = 3.62 VVPITDD449 pKa = 2.96 VSYY452 pKa = 10.96 AIKK455 pKa = 10.02 PLSTIPGDD463 pKa = 3.95 DD464 pKa = 4.69 LLPSSTITMQNGPVHH479 pKa = 7.24 VDD481 pKa = 3.19 GQDD484 pKa = 3.33 NIVPDD489 pKa = 4.42 IIIIDD494 pKa = 3.99 FSVEE498 pKa = 3.28 GTYY501 pKa = 10.68 FLNTYY506 pKa = 8.86 AHH508 pKa = 7.08 PSLHH512 pKa = 5.76 KK513 pKa = 9.88 RR514 pKa = 11.84 KK515 pKa = 9.67 KK516 pKa = 9.9 RR517 pKa = 11.84 RR518 pKa = 11.84 LSS520 pKa = 3.29
Molecular weight: 56.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.999
IPC2_protein 4.978
IPC_protein 4.965
Toseland 4.94
ProMoST 5.13
Dawson 4.978
Bjellqvist 5.105
Wikipedia 4.876
Rodwell 4.902
Grimsley 4.876
Solomon 4.978
Lehninger 4.94
Nozaki 5.118
DTASelect 5.283
Thurlkill 4.952
EMBOSS 4.927
Sillero 5.181
Patrickios 4.037
IPC_peptide 4.991
IPC2_peptide 5.181
IPC2.peptide.svr19 5.105
Protein with the highest isoelectric point:
>tr|A6XA92|A6XA92_BPV5 E7 OS=Bos taurus papillomavirus 8 OX=2758968 PE=4 SV=1
MM1 pKa = 7.72 PCFIPSYY8 pKa = 10.83 NFDD11 pKa = 3.81 GLDD14 pKa = 3.52 CLVCKK19 pKa = 10.46 RR20 pKa = 11.84 PLGSYY25 pKa = 10.13 DD26 pKa = 3.55 ALKK29 pKa = 10.94 CKK31 pKa = 8.39 TNKK34 pKa = 9.05 FRR36 pKa = 11.84 RR37 pKa = 11.84 VHH39 pKa = 6.68 RR40 pKa = 11.84 DD41 pKa = 2.6 GHH43 pKa = 6.24 VYY45 pKa = 10.71 GMCCGCTEE53 pKa = 4.65 LLLKK57 pKa = 10.13 IEE59 pKa = 4.84 RR60 pKa = 11.84 EE61 pKa = 4.17 DD62 pKa = 3.93 NPWSLVCPHH71 pKa = 7.07 DD72 pKa = 3.84 FKK74 pKa = 11.41 KK75 pKa = 11.1 VFGRR79 pKa = 11.84 QVGDD83 pKa = 3.95 CIVRR87 pKa = 11.84 CYY89 pKa = 11.22 YY90 pKa = 10.29 CGCPLSDD97 pKa = 3.93 SEE99 pKa = 4.8 KK100 pKa = 10.64 DD101 pKa = 3.2 RR102 pKa = 11.84 HH103 pKa = 5.69 ALASEE108 pKa = 5.29 GYY110 pKa = 10.03 LFLRR114 pKa = 11.84 GKK116 pKa = 9.72 PRR118 pKa = 11.84 GRR120 pKa = 11.84 CYY122 pKa = 11.05 ACASDD127 pKa = 3.77 GRR129 pKa = 11.84 RR130 pKa = 11.84 PCLYY134 pKa = 10.67
Molecular weight: 15.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.278
IPC2_protein 7.688
IPC_protein 7.468
Toseland 6.912
ProMoST 8.17
Dawson 8.17
Bjellqvist 8.726
Wikipedia 8.068
Rodwell 8.185
Grimsley 6.883
Solomon 8.229
Lehninger 8.244
Nozaki 9.048
DTASelect 8.331
Thurlkill 8.375
EMBOSS 8.375
Sillero 8.77
Patrickios 2.219
IPC_peptide 8.214
IPC2_peptide 8.214
IPC2.peptide.svr19 8.221
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
2439
40
593
304.9
33.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.191 ± 0.588
3.034 ± 1.195
5.74 ± 0.409
5.453 ± 0.395
4.223 ± 0.417
7.749 ± 0.905
2.788 ± 0.523
3.772 ± 0.384
4.592 ± 0.685
8.979 ± 0.829
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.517 ± 0.238
3.895 ± 0.743
6.765 ± 0.943
4.059 ± 0.693
6.191 ± 0.754
7.626 ± 0.687
6.56 ± 0.558
6.56 ± 0.637
1.312 ± 0.238
2.993 ± 0.415
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here