Roseateles aquatilis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiales genera incertae sedis; Roseateles

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5438 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A246JCN2|A0A246JCN2_9BURK Uncharacterized protein OS=Roseateles aquatilis OX=431061 GN=CDN99_13265 PE=4 SV=1
MM1 pKa = 6.56TTAEE5 pKa = 4.36PPGADD10 pKa = 3.54SAADD14 pKa = 3.57DD15 pKa = 4.13PMPRR19 pKa = 11.84PPEE22 pKa = 3.82QPDD25 pKa = 2.79IDD27 pKa = 3.92ACCGNGCDD35 pKa = 3.55PCIFDD40 pKa = 4.89LHH42 pKa = 6.98DD43 pKa = 3.96LAMDD47 pKa = 4.03QYY49 pKa = 11.01RR50 pKa = 11.84QDD52 pKa = 3.26LRR54 pKa = 11.84AWNARR59 pKa = 11.84HH60 pKa = 5.85PQEE63 pKa = 4.01QQ64 pKa = 3.16

Molecular weight: 7.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A246JF42|A0A246JF42_9BURK Fis family transcriptional regulator OS=Roseateles aquatilis OX=431061 GN=CDN99_12000 PE=4 SV=1
MM1 pKa = 7.44PRR3 pKa = 11.84SPPRR7 pKa = 11.84PRR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84LSLPSRR17 pKa = 11.84RR18 pKa = 11.84QARR21 pKa = 11.84HH22 pKa = 5.82RR23 pKa = 11.84SHH25 pKa = 6.97RR26 pKa = 11.84WPRR29 pKa = 11.84PRR31 pKa = 11.84RR32 pKa = 11.84PPRR35 pKa = 11.84PRR37 pKa = 11.84SPRR40 pKa = 11.84PPRR43 pKa = 11.84LPPPTRR49 pKa = 11.84PCPRR53 pKa = 11.84RR54 pKa = 11.84PSHH57 pKa = 6.24WPGPLNPPGRR67 pKa = 11.84SCPPSRR73 pKa = 11.84RR74 pKa = 11.84RR75 pKa = 3.36

Molecular weight: 8.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5438

0

5438

1868886

24

3663

343.7

37.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.309 ± 0.043

0.828 ± 0.01

5.613 ± 0.023

5.126 ± 0.033

3.189 ± 0.022

8.452 ± 0.036

2.157 ± 0.017

3.913 ± 0.02

2.86 ± 0.025

11.063 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.326 ± 0.017

2.332 ± 0.022

5.514 ± 0.029

4.026 ± 0.024

7.679 ± 0.031

5.484 ± 0.031

5.184 ± 0.032

7.412 ± 0.029

1.529 ± 0.014

2.002 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski