Filibacter tadaridae
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3039 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3P5X1R8|A0A3P5X1R8_9BACL Transposase DDE domain protein OS=Filibacter tadaridae OX=2483811 GN=FILTAD_01195 PE=4 SV=1
MM1 pKa = 7.64 RR2 pKa = 11.84 NILIAYY8 pKa = 8.33 TSMTGNTEE16 pKa = 3.82 EE17 pKa = 4.18 LAEE20 pKa = 4.05 IAKK23 pKa = 10.11 VVLTGRR29 pKa = 11.84 GFDD32 pKa = 3.51 VTVKK36 pKa = 10.07 TFDD39 pKa = 3.81 AEE41 pKa = 4.24 AIAAKK46 pKa = 10.32 EE47 pKa = 3.58 FLAYY51 pKa = 10.35 DD52 pKa = 4.73 GILFGTYY59 pKa = 9.37 TYY61 pKa = 11.44 DD62 pKa = 4.99 DD63 pKa = 4.05 GNLPYY68 pKa = 10.05 EE69 pKa = 4.18 IEE71 pKa = 4.09 EE72 pKa = 4.66 TYY74 pKa = 11.54 DD75 pKa = 3.93 DD76 pKa = 5.28 LDD78 pKa = 3.9 TVSFTDD84 pKa = 3.24 KK85 pKa = 10.74 VVGVFGSGDD94 pKa = 2.99 SCYY97 pKa = 11.06 YY98 pKa = 9.24 EE99 pKa = 4.57 FCHH102 pKa = 6.19 ATDD105 pKa = 4.09 LMAEE109 pKa = 4.35 KK110 pKa = 9.01 FTEE113 pKa = 4.39 VGAHH117 pKa = 4.81 VVDD120 pKa = 5.12 SIVKK124 pKa = 9.77 VDD126 pKa = 5.44 LDD128 pKa = 3.72 AQQADD133 pKa = 4.2 VDD135 pKa = 4.1 RR136 pKa = 11.84 MQAMVNMFCDD146 pKa = 4.98 AIDD149 pKa = 3.42 QRR151 pKa = 11.84 LEE153 pKa = 3.99 DD154 pKa = 3.51 SCAA157 pKa = 3.2
Molecular weight: 17.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.715
IPC2_protein 3.872
IPC_protein 3.859
Toseland 3.643
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.783
Rodwell 3.694
Grimsley 3.554
Solomon 3.846
Lehninger 3.808
Nozaki 3.973
DTASelect 4.202
Thurlkill 3.706
EMBOSS 3.795
Sillero 3.986
Patrickios 1.1
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.873
Protein with the highest isoelectric point:
>tr|A0A3P5X1L9|A0A3P5X1L9_9BACL Uncharacterized protein OS=Filibacter tadaridae OX=2483811 GN=FILTAD_01828 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.34 QPNKK9 pKa = 8.21 RR10 pKa = 11.84 KK11 pKa = 9.62 RR12 pKa = 11.84 SKK14 pKa = 9.83 VHH16 pKa = 5.77 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSSKK25 pKa = 9.85 SGRR28 pKa = 11.84 RR29 pKa = 11.84 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 SKK37 pKa = 10.38 GRR39 pKa = 11.84 KK40 pKa = 8.51 VLSAA44 pKa = 4.05
Molecular weight: 5.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.414
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.384
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.021
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3039
0
3039
876302
29
1637
288.4
32.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.472 ± 0.048
0.622 ± 0.013
5.369 ± 0.037
7.45 ± 0.055
4.605 ± 0.036
7.123 ± 0.036
2.014 ± 0.021
7.752 ± 0.044
6.606 ± 0.039
9.616 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.988 ± 0.024
4.038 ± 0.032
3.628 ± 0.027
3.307 ± 0.029
4.236 ± 0.036
5.958 ± 0.032
5.782 ± 0.034
7.235 ± 0.042
0.959 ± 0.016
3.238 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here