Caldisphaera lagunensis (strain DSM 15908 / JCM 11604 / ANMR 0165 / IC-154)
Average proteome isoelectric point is 7.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1477 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|L0A9F8|L0A9F8_CALLD Membrane-bound serine protease (ClpP class) OS=Caldisphaera lagunensis (strain DSM 15908 / JCM 11604 / ANMR 0165 / IC-154) OX=1056495 GN=Calag_0762 PE=4 SV=1
MM1 pKa = 7.25 TCYY4 pKa = 10.11 IDD6 pKa = 4.58 LDD8 pKa = 4.01 NVNDD12 pKa = 4.07 LDD14 pKa = 5.01 EE15 pKa = 6.4 ISDD18 pKa = 3.85 EE19 pKa = 4.44 LNDD22 pKa = 3.68 FAEE25 pKa = 5.64 DD26 pKa = 3.01 ISTIQDD32 pKa = 3.53 YY33 pKa = 11.17 LDD35 pKa = 3.17 EE36 pKa = 5.35 HH37 pKa = 6.48 MGDD40 pKa = 4.6 FIEE43 pKa = 5.24 KK44 pKa = 10.4 LNEE47 pKa = 3.94 ALNSEE52 pKa = 4.78 GIVLSCNEE60 pKa = 3.82 AKK62 pKa = 10.2 EE63 pKa = 4.44 GNMEE67 pKa = 4.38 CKK69 pKa = 10.58 YY70 pKa = 10.69 KK71 pKa = 10.66 FQSDD75 pKa = 4.74 LIGGEE80 pKa = 4.18 VIFLVEE86 pKa = 5.26 IDD88 pKa = 4.16 PFDD91 pKa = 4.63 NKK93 pKa = 10.52 IKK95 pKa = 10.74 DD96 pKa = 3.55 YY97 pKa = 11.11 DD98 pKa = 3.39 IDD100 pKa = 4.41 KK101 pKa = 10.76 IRR103 pKa = 11.84 LITKK107 pKa = 9.48
Molecular weight: 12.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.095
IPC2_protein 3.846
IPC_protein 3.834
Toseland 3.617
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.745
Rodwell 3.656
Grimsley 3.528
Solomon 3.808
Lehninger 3.757
Nozaki 3.935
DTASelect 4.151
Thurlkill 3.668
EMBOSS 3.757
Sillero 3.948
Patrickios 3.261
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.875
Protein with the highest isoelectric point:
>tr|L0A8L4|L0A8L4_CALLD 50S ribosomal protein L15e OS=Caldisphaera lagunensis (strain DSM 15908 / JCM 11604 / ANMR 0165 / IC-154) OX=1056495 GN=rpl15e PE=3 SV=1
MM1 pKa = 7.58 ARR3 pKa = 11.84 NKK5 pKa = 10.21 HH6 pKa = 5.33 LARR9 pKa = 11.84 KK10 pKa = 9.14 LRR12 pKa = 11.84 LAKK15 pKa = 10.24 AGKK18 pKa = 9.75 SNEE21 pKa = 4.29 SVPVWVVVKK30 pKa = 7.84 TVRR33 pKa = 11.84 KK34 pKa = 7.51 FTRR37 pKa = 11.84 NPKK40 pKa = 8.56 SRR42 pKa = 11.84 NWRR45 pKa = 11.84 TSKK48 pKa = 10.98 LKK50 pKa = 10.6 VV51 pKa = 3.09
Molecular weight: 5.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 10.716
IPC_protein 12.237
Toseland 12.427
ProMoST 12.896
Dawson 12.427
Bjellqvist 12.413
Wikipedia 12.896
Rodwell 12.34
Grimsley 12.471
Solomon 12.91
Lehninger 12.808
Nozaki 12.427
DTASelect 12.413
Thurlkill 12.427
EMBOSS 12.925
Sillero 12.427
Patrickios 12.076
IPC_peptide 12.91
IPC2_peptide 11.886
IPC2.peptide.svr19 9.015
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1477
0
1477
442436
32
1405
299.6
33.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.689 ± 0.058
0.618 ± 0.019
4.848 ± 0.055
6.681 ± 0.073
4.099 ± 0.054
6.476 ± 0.059
1.228 ± 0.022
10.833 ± 0.078
8.346 ± 0.092
9.882 ± 0.071
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.362 ± 0.024
6.203 ± 0.068
3.971 ± 0.047
1.96 ± 0.031
3.57 ± 0.057
7.021 ± 0.076
4.362 ± 0.058
5.998 ± 0.044
1.009 ± 0.024
4.844 ± 0.061
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here