Amino acid dipepetide frequency for Caldisphaera lagunensis (strain DSM 15908 / JCM 11604 / ANMR 0165 / IC-154)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.745AlaAla: 3.745 ± 0.127
0.298AlaCys: 0.298 ± 0.03
2.335AlaAsp: 2.335 ± 0.071
3.214AlaGlu: 3.214 ± 0.089
2.561AlaPhe: 2.561 ± 0.083
3.734AlaGly: 3.734 ± 0.132
0.773AlaHis: 0.773 ± 0.045
6.46AlaIle: 6.46 ± 0.148
4.661AlaLys: 4.661 ± 0.115
6.568AlaLeu: 6.568 ± 0.153
1.571AlaMet: 1.571 ± 0.069
2.699AlaAsn: 2.699 ± 0.084
1.589AlaPro: 1.589 ± 0.064
1.202AlaGln: 1.202 ± 0.059
2.168AlaArg: 2.168 ± 0.08
4.084AlaSer: 4.084 ± 0.113
2.416AlaThr: 2.416 ± 0.074
3.512AlaVal: 3.512 ± 0.108
0.57AlaTrp: 0.57 ± 0.037
2.735AlaTyr: 2.735 ± 0.092
0.0AlaXaa: 0.0 ± 0.0
Cys
0.307CysAla: 0.307 ± 0.03
0.084CysCys: 0.084 ± 0.015
0.328CysAsp: 0.328 ± 0.026
0.355CysGlu: 0.355 ± 0.032
0.179CysPhe: 0.179 ± 0.024
0.692CysGly: 0.692 ± 0.049
0.081CysHis: 0.081 ± 0.014
0.518CysIle: 0.518 ± 0.039
0.565CysLys: 0.565 ± 0.048
0.425CysLeu: 0.425 ± 0.033
0.145CysMet: 0.145 ± 0.017
0.493CysAsn: 0.493 ± 0.037
0.599CysPro: 0.599 ± 0.045
0.118CysGln: 0.118 ± 0.017
0.185CysArg: 0.185 ± 0.026
0.348CysSer: 0.348 ± 0.03
0.208CysThr: 0.208 ± 0.022
0.271CysVal: 0.271 ± 0.03
0.043CysTrp: 0.043 ± 0.01
0.24CysTyr: 0.24 ± 0.027
0.0CysXaa: 0.0 ± 0.0
Asp
2.696AspAla: 2.696 ± 0.077
0.276AspCys: 0.276 ± 0.031
2.138AspAsp: 2.138 ± 0.072
3.761AspGlu: 3.761 ± 0.109
2.183AspPhe: 2.183 ± 0.07
2.47AspGly: 2.47 ± 0.084
0.597AspHis: 0.597 ± 0.036
5.397AspIle: 5.397 ± 0.131
4.109AspLys: 4.109 ± 0.121
5.194AspLeu: 5.194 ± 0.127
1.2AspMet: 1.2 ± 0.059
2.629AspAsn: 2.629 ± 0.087
2.382AspPro: 2.382 ± 0.079
0.913AspGln: 0.913 ± 0.045
1.503AspArg: 1.503 ± 0.064
2.658AspSer: 2.658 ± 0.084
1.786AspThr: 1.786 ± 0.076
3.515AspVal: 3.515 ± 0.102
0.493AspTrp: 0.493 ± 0.031
2.579AspTyr: 2.579 ± 0.088
0.0AspXaa: 0.0 ± 0.0
Glu
3.897GluAla: 3.897 ± 0.11
0.335GluCys: 0.335 ± 0.03
3.173GluAsp: 3.173 ± 0.103
5.741GluGlu: 5.741 ± 0.152
2.694GluPhe: 2.694 ± 0.086
3.804GluGly: 3.804 ± 0.118
0.613GluHis: 0.613 ± 0.039
7.617GluIle: 7.617 ± 0.184
6.871GluLys: 6.871 ± 0.157
6.392GluLeu: 6.392 ± 0.139
1.537GluMet: 1.537 ± 0.059
4.602GluAsn: 4.602 ± 0.105
1.973GluPro: 1.973 ± 0.071
1.187GluGln: 1.187 ± 0.056
2.565GluArg: 2.565 ± 0.081
3.833GluSer: 3.833 ± 0.105
2.778GluThr: 2.778 ± 0.094
4.03GluVal: 4.03 ± 0.103
0.624GluTrp: 0.624 ± 0.043
2.547GluTyr: 2.547 ± 0.08
0.0GluXaa: 0.0 ± 0.0
Phe
2.375PheAla: 2.375 ± 0.096
0.262PheCys: 0.262 ± 0.031
2.156PheAsp: 2.156 ± 0.075
2.1PheGlu: 2.1 ± 0.078
1.584PhePhe: 1.584 ± 0.074
2.703PheGly: 2.703 ± 0.087
0.665PheHis: 0.665 ± 0.038
4.315PheIle: 4.315 ± 0.13
2.95PheLys: 2.95 ± 0.082
4.068PheLeu: 4.068 ± 0.113
1.008PheMet: 1.008 ± 0.054
2.787PheAsn: 2.787 ± 0.1
1.56PhePro: 1.56 ± 0.063
0.676PheGln: 0.676 ± 0.041
1.318PheArg: 1.318 ± 0.058
3.384PheSer: 3.384 ± 0.109
1.937PheThr: 1.937 ± 0.072
2.608PheVal: 2.608 ± 0.081
0.382PheTrp: 0.382 ± 0.032
2.152PheTyr: 2.152 ± 0.08
0.0PheXaa: 0.0 ± 0.0
Gly
3.478GlyAla: 3.478 ± 0.107
0.475GlyCys: 0.475 ± 0.035
2.841GlyAsp: 2.841 ± 0.076
3.971GlyGlu: 3.971 ± 0.098
3.463GlyPhe: 3.463 ± 0.087
4.448GlyGly: 4.448 ± 0.125
0.938GlyHis: 0.938 ± 0.048
7.56GlyIle: 7.56 ± 0.157
5.341GlyLys: 5.341 ± 0.121
6.654GlyLeu: 6.654 ± 0.141
1.424GlyMet: 1.424 ± 0.059
3.361GlyAsn: 3.361 ± 0.109
2.084GlyPro: 2.084 ± 0.065
1.119GlyGln: 1.119 ± 0.054
2.31GlyArg: 2.31 ± 0.076
4.001GlySer: 4.001 ± 0.107
2.909GlyThr: 2.909 ± 0.092
4.15GlyVal: 4.15 ± 0.107
0.764GlyTrp: 0.764 ± 0.053
3.467GlyTyr: 3.467 ± 0.099
0.0GlyXaa: 0.0 ± 0.0
His
0.802HisAla: 0.802 ± 0.048
0.099HisCys: 0.099 ± 0.013
0.558HisAsp: 0.558 ± 0.034
0.818HisGlu: 0.818 ± 0.044
0.513HisPhe: 0.513 ± 0.033
1.022HisGly: 1.022 ± 0.044
0.264HisHis: 0.264 ± 0.028
1.187HisIle: 1.187 ± 0.041
0.759HisLys: 0.759 ± 0.041
1.196HisLeu: 1.196 ± 0.052
0.332HisMet: 0.332 ± 0.023
0.617HisAsn: 0.617 ± 0.043
0.737HisPro: 0.737 ± 0.037
0.208HisGln: 0.208 ± 0.022
0.479HisArg: 0.479 ± 0.035
0.683HisSer: 0.683 ± 0.042
0.504HisThr: 0.504 ± 0.038
0.762HisVal: 0.762 ± 0.044
0.154HisTrp: 0.154 ± 0.021
0.588HisTyr: 0.588 ± 0.039
0.0HisXaa: 0.0 ± 0.0
Ile
6.806IleAla: 6.806 ± 0.157
0.545IleCys: 0.545 ± 0.041
6.134IleAsp: 6.134 ± 0.121
6.862IleGlu: 6.862 ± 0.145
3.921IlePhe: 3.921 ± 0.119
6.848IleGly: 6.848 ± 0.144
1.166IleHis: 1.166 ± 0.056
11.948IleIle: 11.948 ± 0.211
9.283IleLys: 9.283 ± 0.204
9.662IleLeu: 9.662 ± 0.173
2.455IleMet: 2.455 ± 0.069
7.402IleAsn: 7.402 ± 0.161
4.374IlePro: 4.374 ± 0.106
1.675IleGln: 1.675 ± 0.055
3.601IleArg: 3.601 ± 0.086
8.706IleSer: 8.706 ± 0.183
4.984IleThr: 4.984 ± 0.112
6.433IleVal: 6.433 ± 0.117
0.739IleTrp: 0.739 ± 0.044
4.789IleTyr: 4.789 ± 0.116
0.0IleXaa: 0.0 ± 0.0
Lys
4.392LysAla: 4.392 ± 0.102
0.583LysCys: 0.583 ± 0.041
4.416LysAsp: 4.416 ± 0.133
7.725LysGlu: 7.725 ± 0.186
2.667LysPhe: 2.667 ± 0.092
5.255LysGly: 5.255 ± 0.12
0.802LysHis: 0.802 ± 0.038
9.748LysIle: 9.748 ± 0.195
7.016LysLys: 7.016 ± 0.18
7.371LysLeu: 7.371 ± 0.146
2.145LysMet: 2.145 ± 0.067
5.687LysAsn: 5.687 ± 0.129
2.997LysPro: 2.997 ± 0.086
1.693LysGln: 1.693 ± 0.058
3.103LysArg: 3.103 ± 0.1
4.699LysSer: 4.699 ± 0.107
3.334LysThr: 3.334 ± 0.083
5.034LysVal: 5.034 ± 0.13
0.789LysTrp: 0.789 ± 0.044
4.005LysTyr: 4.005 ± 0.098
0.0LysXaa: 0.0 ± 0.0
Leu
6.021LeuAla: 6.021 ± 0.149
0.461LeuCys: 0.461 ± 0.036
4.855LeuAsp: 4.855 ± 0.121
5.879LeuGlu: 5.879 ± 0.122
3.833LeuPhe: 3.833 ± 0.115
6.659LeuGly: 6.659 ± 0.133
1.04LeuHis: 1.04 ± 0.048
11.125LeuIle: 11.125 ± 0.209
8.198LeuLys: 8.198 ± 0.158
9.319LeuLeu: 9.319 ± 0.22
2.238LeuMet: 2.238 ± 0.083
6.521LeuAsn: 6.521 ± 0.163
3.869LeuPro: 3.869 ± 0.102
1.772LeuGln: 1.772 ± 0.076
3.894LeuArg: 3.894 ± 0.11
8.153LeuSer: 8.153 ± 0.171
4.403LeuThr: 4.403 ± 0.114
5.253LeuVal: 5.253 ± 0.118
0.868LeuTrp: 0.868 ± 0.046
4.464LeuTyr: 4.464 ± 0.113
0.0LeuXaa: 0.0 ± 0.0
Met
1.519MetAla: 1.519 ± 0.056
0.093MetCys: 0.093 ± 0.014
1.083MetAsp: 1.083 ± 0.049
1.756MetGlu: 1.756 ± 0.065
0.816MetPhe: 0.816 ± 0.044
1.779MetGly: 1.779 ± 0.07
0.348MetHis: 0.348 ± 0.028
2.4MetIle: 2.4 ± 0.085
2.12MetLys: 2.12 ± 0.072
2.027MetLeu: 2.027 ± 0.062
0.504MetMet: 0.504 ± 0.034
1.225MetAsn: 1.225 ± 0.05
1.241MetPro: 1.241 ± 0.047
0.558MetGln: 0.558 ± 0.033
1.051MetArg: 1.051 ± 0.053
1.754MetSer: 1.754 ± 0.067
0.961MetThr: 0.961 ± 0.051
1.435MetVal: 1.435 ± 0.056
0.194MetTrp: 0.194 ± 0.022
0.753MetTyr: 0.753 ± 0.05
0.0MetXaa: 0.0 ± 0.0
Asn
3.295AsnAla: 3.295 ± 0.084
0.486AsnCys: 0.486 ± 0.034
3.155AsnAsp: 3.155 ± 0.091
4.807AsnGlu: 4.807 ± 0.117
2.195AsnPhe: 2.195 ± 0.074
3.564AsnGly: 3.564 ± 0.116
0.579AsnHis: 0.579 ± 0.04
6.564AsnIle: 6.564 ± 0.14
5.87AsnLys: 5.87 ± 0.148
5.904AsnLeu: 5.904 ± 0.122
1.331AsnMet: 1.331 ± 0.054
4.403AsnAsn: 4.403 ± 0.127
2.715AsnPro: 2.715 ± 0.086
1.349AsnGln: 1.349 ± 0.056
1.736AsnArg: 1.736 ± 0.072
4.041AsnSer: 4.041 ± 0.115
2.464AsnThr: 2.464 ± 0.088
3.827AsnVal: 3.827 ± 0.104
0.527AsnTrp: 0.527 ± 0.036
3.219AsnTyr: 3.219 ± 0.094
0.0AsnXaa: 0.0 ± 0.0
Pro
1.711ProAla: 1.711 ± 0.067
0.233ProCys: 0.233 ± 0.023
1.912ProAsp: 1.912 ± 0.073
2.739ProGlu: 2.739 ± 0.092
1.752ProPhe: 1.752 ± 0.068
2.73ProGly: 2.73 ± 0.095
0.531ProHis: 0.531 ± 0.031
4.057ProIle: 4.057 ± 0.099
3.011ProLys: 3.011 ± 0.084
3.879ProLeu: 3.879 ± 0.098
0.841ProMet: 0.841 ± 0.04
2.265ProAsn: 2.265 ± 0.086
1.67ProPro: 1.67 ± 0.07
0.825ProGln: 0.825 ± 0.045
1.381ProArg: 1.381 ± 0.057
3.031ProSer: 3.031 ± 0.089
1.928ProThr: 1.928 ± 0.08
2.427ProVal: 2.427 ± 0.076
0.597ProTrp: 0.597 ± 0.044
2.19ProTyr: 2.19 ± 0.077
0.0ProXaa: 0.0 ± 0.0
Gln
1.282GlnAla: 1.282 ± 0.063
0.102GlnCys: 0.102 ± 0.014
0.845GlnAsp: 0.845 ± 0.047
1.254GlnGlu: 1.254 ± 0.053
0.753GlnPhe: 0.753 ± 0.045
1.232GlnGly: 1.232 ± 0.059
0.194GlnHis: 0.194 ± 0.019
1.989GlnIle: 1.989 ± 0.072
1.453GlnLys: 1.453 ± 0.061
1.991GlnLeu: 1.991 ± 0.073
0.477GlnMet: 0.477 ± 0.034
1.257GlnAsn: 1.257 ± 0.05
0.649GlnPro: 0.649 ± 0.041
0.649GlnGln: 0.649 ± 0.045
0.748GlnArg: 0.748 ± 0.04
1.302GlnSer: 1.302 ± 0.059
1.006GlnThr: 1.006 ± 0.056
1.182GlnVal: 1.182 ± 0.059
0.258GlnTrp: 0.258 ± 0.023
0.979GlnTyr: 0.979 ± 0.053
0.0GlnXaa: 0.0 ± 0.0
Arg
2.007ArgAla: 2.007 ± 0.076
0.325ArgCys: 0.325 ± 0.036
1.761ArgAsp: 1.761 ± 0.069
2.638ArgGlu: 2.638 ± 0.082
1.471ArgPhe: 1.471 ± 0.06
2.285ArgGly: 2.285 ± 0.075
0.542ArgHis: 0.542 ± 0.036
3.494ArgIle: 3.494 ± 0.098
3.248ArgLys: 3.248 ± 0.077
3.601ArgLeu: 3.601 ± 0.099
0.744ArgMet: 0.744 ± 0.049
2.057ArgAsn: 2.057 ± 0.073
1.537ArgPro: 1.537 ± 0.066
0.689ArgGln: 0.689 ± 0.041
1.697ArgArg: 1.697 ± 0.064
2.109ArgSer: 2.109 ± 0.076
1.209ArgThr: 1.209 ± 0.058
2.22ArgVal: 2.22 ± 0.077
0.398ArgTrp: 0.398 ± 0.03
1.666ArgTyr: 1.666 ± 0.055
0.0ArgXaa: 0.0 ± 0.0
Ser
3.035SerAla: 3.035 ± 0.093
0.509SerCys: 0.509 ± 0.037
2.882SerAsp: 2.882 ± 0.099
3.616SerGlu: 3.616 ± 0.102
3.241SerPhe: 3.241 ± 0.094
4.312SerGly: 4.312 ± 0.111
0.958SerHis: 0.958 ± 0.048
7.558SerIle: 7.558 ± 0.162
5.723SerLys: 5.723 ± 0.13
8.487SerLeu: 8.487 ± 0.166
1.831SerMet: 1.831 ± 0.069
4.285SerAsn: 4.285 ± 0.124
2.604SerPro: 2.604 ± 0.079
1.749SerGln: 1.749 ± 0.076
2.421SerArg: 2.421 ± 0.068
5.648SerSer: 5.648 ± 0.186
3.241SerThr: 3.241 ± 0.126
3.551SerVal: 3.551 ± 0.101
0.827SerTrp: 0.827 ± 0.042
3.727SerTyr: 3.727 ± 0.105
0.0SerXaa: 0.0 ± 0.0
Thr
2.563ThrAla: 2.563 ± 0.098
0.244ThrCys: 0.244 ± 0.024
1.627ThrAsp: 1.627 ± 0.056
1.973ThrGlu: 1.973 ± 0.061
1.932ThrPhe: 1.932 ± 0.077
3.395ThrGly: 3.395 ± 0.105
0.644ThrHis: 0.644 ± 0.045
4.591ThrIle: 4.591 ± 0.107
2.918ThrLys: 2.918 ± 0.081
4.468ThrLeu: 4.468 ± 0.111
1.06ThrMet: 1.06 ± 0.047
2.344ThrAsn: 2.344 ± 0.069
2.251ThrPro: 2.251 ± 0.096
1.022ThrGln: 1.022 ± 0.053
1.424ThrArg: 1.424 ± 0.061
3.28ThrSer: 3.28 ± 0.121
2.59ThrThr: 2.59 ± 0.154
2.565ThrVal: 2.565 ± 0.097
0.515ThrTrp: 0.515 ± 0.033
2.213ThrTyr: 2.213 ± 0.082
0.0ThrXaa: 0.0 ± 0.0
Val
3.54ValAla: 3.54 ± 0.103
0.355ValCys: 0.355 ± 0.035
3.158ValAsp: 3.158 ± 0.094
3.899ValGlu: 3.899 ± 0.11
2.55ValPhe: 2.55 ± 0.084
3.804ValGly: 3.804 ± 0.101
0.707ValHis: 0.707 ± 0.043
6.369ValIle: 6.369 ± 0.136
5.067ValLys: 5.067 ± 0.129
5.524ValLeu: 5.524 ± 0.117
1.372ValMet: 1.372 ± 0.055
3.456ValAsn: 3.456 ± 0.104
2.489ValPro: 2.489 ± 0.08
0.9ValGln: 0.9 ± 0.046
2.095ValArg: 2.095 ± 0.087
4.704ValSer: 4.704 ± 0.117
2.581ValThr: 2.581 ± 0.089
3.888ValVal: 3.888 ± 0.105
0.576ValTrp: 0.576 ± 0.037
2.95ValTyr: 2.95 ± 0.075
0.0ValXaa: 0.0 ± 0.0
Trp
0.628TrpAla: 0.628 ± 0.044
0.054TrpCys: 0.054 ± 0.011
0.57TrpAsp: 0.57 ± 0.044
0.671TrpGlu: 0.671 ± 0.043
0.459TrpPhe: 0.459 ± 0.038
0.703TrpGly: 0.703 ± 0.039
0.163TrpHis: 0.163 ± 0.02
1.028TrpIle: 1.028 ± 0.046
0.732TrpLys: 0.732 ± 0.037
0.983TrpLeu: 0.983 ± 0.052
0.219TrpMet: 0.219 ± 0.022
0.579TrpAsn: 0.579 ± 0.041
0.418TrpPro: 0.418 ± 0.036
0.215TrpGln: 0.215 ± 0.023
0.475TrpArg: 0.475 ± 0.031
0.597TrpSer: 0.597 ± 0.034
0.285TrpThr: 0.285 ± 0.022
0.603TrpVal: 0.603 ± 0.043
0.197TrpTrp: 0.197 ± 0.027
0.506TrpTyr: 0.506 ± 0.038
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.794TyrAla: 2.794 ± 0.096
0.364TyrCys: 0.364 ± 0.034
2.552TyrAsp: 2.552 ± 0.086
3.031TyrGlu: 3.031 ± 0.091
2.222TyrPhe: 2.222 ± 0.079
3.318TyrGly: 3.318 ± 0.077
0.676TyrHis: 0.676 ± 0.036
4.407TyrIle: 4.407 ± 0.103
3.569TyrLys: 3.569 ± 0.107
5.11TyrLeu: 5.11 ± 0.113
1.182TyrMet: 1.182 ± 0.061
3.363TyrAsn: 3.363 ± 0.097
1.973TyrPro: 1.973 ± 0.06
1.083TyrGln: 1.083 ± 0.061
1.544TyrArg: 1.544 ± 0.061
3.194TyrSer: 3.194 ± 0.092
2.097TyrThr: 2.097 ± 0.093
2.717TyrVal: 2.717 ± 0.077
0.572TyrTrp: 0.572 ± 0.044
2.672TyrTyr: 2.672 ± 0.094
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1477 proteins (442436 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski