Pseudothermotoga hypogea DSM 11164 = NBRC 106472

Taxonomy: cellular organisms; Bacteria; Thermotogae; Thermotogae; Thermotogales; Thermotogaceae; Pseudothermotoga; Pseudothermotoga hypogea

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2094 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0X1KQ37|A0A0X1KQ37_9THEM NADH dehydrogenase OS=Pseudothermotoga hypogea DSM 11164 = NBRC 106472 OX=1123384 GN=AJ81_03360 PE=4 SV=1
MM1 pKa = 7.37GGWKK5 pKa = 9.84RR6 pKa = 11.84CPQCGEE12 pKa = 3.66KK13 pKa = 10.45CRR15 pKa = 11.84NNVRR19 pKa = 11.84KK20 pKa = 9.74CPSCGYY26 pKa = 9.78VFEE29 pKa = 5.37KK30 pKa = 10.72KK31 pKa = 9.76EE32 pKa = 4.15CPDD35 pKa = 3.83CGAVIDD41 pKa = 5.38DD42 pKa = 6.06DD43 pKa = 4.87IDD45 pKa = 3.52EE46 pKa = 4.83CPICGWLFSDD56 pKa = 4.31EE57 pKa = 4.81FFDD60 pKa = 6.03EE61 pKa = 4.34RR62 pKa = 11.84DD63 pKa = 2.99EE64 pKa = 5.42DD65 pKa = 4.22YY66 pKa = 10.94DD67 pKa = 4.72YY68 pKa = 11.96EE69 pKa = 4.4DD70 pKa = 5.88LEE72 pKa = 4.89DD73 pKa = 4.35FHH75 pKa = 8.7DD76 pKa = 4.4LSDD79 pKa = 5.18YY80 pKa = 11.15EE81 pKa = 5.81DD82 pKa = 3.84EE83 pKa = 4.7DD84 pKa = 3.81

Molecular weight: 9.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0X1KTH7|A0A0X1KTH7_9THEM FeS cluster assembly scaffold IscU OS=Pseudothermotoga hypogea DSM 11164 = NBRC 106472 OX=1123384 GN=AJ81_10270 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.21QPNRR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 9.76RR12 pKa = 11.84QKK14 pKa = 8.17THH16 pKa = 5.19GFLVRR21 pKa = 11.84SRR23 pKa = 11.84TASGRR28 pKa = 11.84KK29 pKa = 8.42VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.49GRR39 pKa = 11.84WRR41 pKa = 11.84LAVV44 pKa = 3.35

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2094

0

2094

670145

31

1650

320.0

36.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.064 ± 0.048

0.954 ± 0.017

4.795 ± 0.033

7.427 ± 0.064

5.023 ± 0.045

6.614 ± 0.044

1.679 ± 0.021

6.8 ± 0.041

6.63 ± 0.041

10.77 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.571 ± 0.023

3.589 ± 0.026

3.878 ± 0.035

2.909 ± 0.033

5.496 ± 0.04

6.006 ± 0.045

4.658 ± 0.034

8.722 ± 0.037

1.068 ± 0.024

3.347 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski