Amino acid dipepetide frequency for Pseudothermotoga hypogea DSM 11164 = NBRC 106472

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.248AlaAla: 5.248 ± 0.122
0.715AlaCys: 0.715 ± 0.035
2.71AlaAsp: 2.71 ± 0.063
4.424AlaGlu: 4.424 ± 0.09
3.357AlaPhe: 3.357 ± 0.075
4.899AlaGly: 4.899 ± 0.099
1.189AlaHis: 1.189 ± 0.043
5.511AlaIle: 5.511 ± 0.1
4.853AlaLys: 4.853 ± 0.082
8.416AlaLeu: 8.416 ± 0.124
2.106AlaMet: 2.106 ± 0.061
2.337AlaAsn: 2.337 ± 0.059
2.025AlaPro: 2.025 ± 0.058
2.398AlaGln: 2.398 ± 0.066
4.041AlaArg: 4.041 ± 0.082
4.235AlaSer: 4.235 ± 0.097
3.159AlaThr: 3.159 ± 0.074
6.055AlaVal: 6.055 ± 0.101
0.631AlaTrp: 0.631 ± 0.038
2.326AlaTyr: 2.326 ± 0.055
0.0AlaXaa: 0.0 ± 0.0
Cys
0.663CysAla: 0.663 ± 0.032
0.094CysCys: 0.094 ± 0.012
0.577CysAsp: 0.577 ± 0.034
0.827CysGlu: 0.827 ± 0.037
0.428CysPhe: 0.428 ± 0.028
0.974CysGly: 0.974 ± 0.04
0.227CysHis: 0.227 ± 0.018
0.491CysIle: 0.491 ± 0.029
0.443CysLys: 0.443 ± 0.028
0.851CysLeu: 0.851 ± 0.039
0.143CysMet: 0.143 ± 0.016
0.282CysAsn: 0.282 ± 0.025
0.525CysPro: 0.525 ± 0.033
0.201CysGln: 0.201 ± 0.017
0.478CysArg: 0.478 ± 0.028
0.585CysSer: 0.585 ± 0.028
0.4CysThr: 0.4 ± 0.028
0.936CysVal: 0.936 ± 0.044
0.133CysTrp: 0.133 ± 0.015
0.281CysTyr: 0.281 ± 0.021
0.0CysXaa: 0.0 ± 0.0
Asp
3.217AspAla: 3.217 ± 0.066
0.409AspCys: 0.409 ± 0.024
1.809AspAsp: 1.809 ± 0.055
4.48AspGlu: 4.48 ± 0.089
2.692AspPhe: 2.692 ± 0.073
3.551AspGly: 3.551 ± 0.074
0.864AspHis: 0.864 ± 0.033
2.905AspIle: 2.905 ± 0.071
2.261AspLys: 2.261 ± 0.062
5.344AspLeu: 5.344 ± 0.097
1.071AspMet: 1.071 ± 0.043
1.137AspAsn: 1.137 ± 0.036
2.765AspPro: 2.765 ± 0.068
1.23AspGln: 1.23 ± 0.046
2.711AspArg: 2.711 ± 0.073
2.373AspSer: 2.373 ± 0.058
1.7AspThr: 1.7 ± 0.052
4.948AspVal: 4.948 ± 0.078
0.686AspTrp: 0.686 ± 0.038
1.801AspTyr: 1.801 ± 0.053
0.0AspXaa: 0.0 ± 0.0
Glu
5.003GluAla: 5.003 ± 0.099
0.512GluCys: 0.512 ± 0.024
3.437GluAsp: 3.437 ± 0.074
6.805GluGlu: 6.805 ± 0.133
2.992GluPhe: 2.992 ± 0.066
4.12GluGly: 4.12 ± 0.093
1.213GluHis: 1.213 ± 0.041
5.209GluIle: 5.209 ± 0.098
6.73GluLys: 6.73 ± 0.128
7.864GluLeu: 7.864 ± 0.118
1.965GluMet: 1.965 ± 0.055
3.119GluAsn: 3.119 ± 0.074
2.543GluPro: 2.543 ± 0.075
2.513GluGln: 2.513 ± 0.068
5.224GluArg: 5.224 ± 0.105
3.596GluSer: 3.596 ± 0.081
3.316GluThr: 3.316 ± 0.078
5.241GluVal: 5.241 ± 0.086
0.691GluTrp: 0.691 ± 0.033
2.176GluTyr: 2.176 ± 0.07
0.0GluXaa: 0.0 ± 0.0
Phe
3.714PheAla: 3.714 ± 0.086
0.533PheCys: 0.533 ± 0.03
2.843PheAsp: 2.843 ± 0.08
4.069PheGlu: 4.069 ± 0.081
2.734PhePhe: 2.734 ± 0.082
3.62PheGly: 3.62 ± 0.081
0.855PheHis: 0.855 ± 0.035
2.59PheIle: 2.59 ± 0.058
2.802PheLys: 2.802 ± 0.065
5.554PheLeu: 5.554 ± 0.128
1.127PheMet: 1.127 ± 0.04
1.677PheAsn: 1.677 ± 0.052
1.731PhePro: 1.731 ± 0.05
1.313PheGln: 1.313 ± 0.042
2.138PheArg: 2.138 ± 0.061
3.713PheSer: 3.713 ± 0.082
2.24PheThr: 2.24 ± 0.064
4.76PheVal: 4.76 ± 0.089
0.573PheTrp: 0.573 ± 0.035
1.641PheTyr: 1.641 ± 0.053
0.0PheXaa: 0.0 ± 0.0
Gly
4.769GlyAla: 4.769 ± 0.091
0.74GlyCys: 0.74 ± 0.037
2.647GlyAsp: 2.647 ± 0.068
4.478GlyGlu: 4.478 ± 0.091
3.684GlyPhe: 3.684 ± 0.088
4.39GlyGly: 4.39 ± 0.104
1.176GlyHis: 1.176 ± 0.041
4.638GlyIle: 4.638 ± 0.096
4.736GlyLys: 4.736 ± 0.089
6.761GlyLeu: 6.761 ± 0.115
1.916GlyMet: 1.916 ± 0.055
2.046GlyAsn: 2.046 ± 0.057
2.031GlyPro: 2.031 ± 0.06
1.794GlyGln: 1.794 ± 0.059
3.381GlyArg: 3.381 ± 0.08
3.834GlySer: 3.834 ± 0.079
3.663GlyThr: 3.663 ± 0.081
5.818GlyVal: 5.818 ± 0.088
0.931GlyTrp: 0.931 ± 0.041
2.702GlyTyr: 2.702 ± 0.073
0.0GlyXaa: 0.0 ± 0.0
His
1.15HisAla: 1.15 ± 0.041
0.178HisCys: 0.178 ± 0.015
0.816HisAsp: 0.816 ± 0.04
1.125HisGlu: 1.125 ± 0.038
0.93HisPhe: 0.93 ± 0.038
1.38HisGly: 1.38 ± 0.045
0.37HisHis: 0.37 ± 0.025
0.979HisIle: 0.979 ± 0.037
0.698HisLys: 0.698 ± 0.031
1.821HisLeu: 1.821 ± 0.045
0.422HisMet: 0.422 ± 0.025
0.47HisAsn: 0.47 ± 0.024
1.013HisPro: 1.013 ± 0.046
0.457HisGln: 0.457 ± 0.028
0.946HisArg: 0.946 ± 0.04
0.931HisSer: 0.931 ± 0.034
0.789HisThr: 0.789 ± 0.034
1.561HisVal: 1.561 ± 0.055
0.179HisTrp: 0.179 ± 0.015
0.576HisTyr: 0.576 ± 0.033
0.0HisXaa: 0.0 ± 0.0
Ile
5.694IleAla: 5.694 ± 0.104
0.636IleCys: 0.636 ± 0.033
4.293IleAsp: 4.293 ± 0.07
5.864IleGlu: 5.864 ± 0.099
3.659IlePhe: 3.659 ± 0.08
4.439IleGly: 4.439 ± 0.095
1.031IleHis: 1.031 ± 0.042
3.475IleIle: 3.475 ± 0.079
3.68IleLys: 3.68 ± 0.084
6.912IleLeu: 6.912 ± 0.103
1.455IleMet: 1.455 ± 0.052
2.126IleAsn: 2.126 ± 0.053
2.837IlePro: 2.837 ± 0.07
1.588IleGln: 1.588 ± 0.05
2.952IleArg: 2.952 ± 0.067
4.036IleSer: 4.036 ± 0.073
3.067IleThr: 3.067 ± 0.068
7.46IleVal: 7.46 ± 0.112
0.609IleTrp: 0.609 ± 0.027
2.188IleTyr: 2.188 ± 0.051
0.0IleXaa: 0.0 ± 0.0
Lys
4.436LysAla: 4.436 ± 0.081
0.452LysCys: 0.452 ± 0.03
3.608LysAsp: 3.608 ± 0.073
4.917LysGlu: 4.917 ± 0.113
2.577LysPhe: 2.577 ± 0.065
3.836LysGly: 3.836 ± 0.079
1.109LysHis: 1.109 ± 0.043
5.457LysIle: 5.457 ± 0.089
5.039LysLys: 5.039 ± 0.087
7.24LysLeu: 7.24 ± 0.121
1.767LysMet: 1.767 ± 0.044
2.801LysAsn: 2.801 ± 0.073
2.568LysPro: 2.568 ± 0.066
2.079LysGln: 2.079 ± 0.059
4.069LysArg: 4.069 ± 0.079
3.235LysSer: 3.235 ± 0.073
3.141LysThr: 3.141 ± 0.075
5.244LysVal: 5.244 ± 0.089
0.577LysTrp: 0.577 ± 0.029
2.149LysTyr: 2.149 ± 0.062
0.0LysXaa: 0.0 ± 0.0
Leu
7.956LeuAla: 7.956 ± 0.128
1.097LeuCys: 1.097 ± 0.04
5.012LeuAsp: 5.012 ± 0.097
7.476LeuGlu: 7.476 ± 0.115
5.047LeuPhe: 5.047 ± 0.12
7.007LeuGly: 7.007 ± 0.124
1.515LeuHis: 1.515 ± 0.043
6.969LeuIle: 6.969 ± 0.115
8.153LeuLys: 8.153 ± 0.126
11.323LeuLeu: 11.323 ± 0.159
2.692LeuMet: 2.692 ± 0.067
4.277LeuAsn: 4.277 ± 0.081
4.157LeuPro: 4.157 ± 0.084
2.935LeuGln: 2.935 ± 0.066
6.139LeuArg: 6.139 ± 0.102
8.071LeuSer: 8.071 ± 0.114
5.288LeuThr: 5.288 ± 0.111
8.535LeuVal: 8.535 ± 0.114
0.967LeuTrp: 0.967 ± 0.042
3.086LeuTyr: 3.086 ± 0.075
0.0LeuXaa: 0.0 ± 0.0
Met
1.794MetAla: 1.794 ± 0.057
0.252MetCys: 0.252 ± 0.02
1.107MetAsp: 1.107 ± 0.042
1.521MetGlu: 1.521 ± 0.051
1.127MetPhe: 1.127 ± 0.042
1.665MetGly: 1.665 ± 0.049
0.31MetHis: 0.31 ± 0.024
2.02MetIle: 2.02 ± 0.059
2.376MetLys: 2.376 ± 0.071
2.449MetLeu: 2.449 ± 0.054
0.676MetMet: 0.676 ± 0.032
1.253MetAsn: 1.253 ± 0.05
0.979MetPro: 0.979 ± 0.037
0.628MetGln: 0.628 ± 0.028
1.858MetArg: 1.858 ± 0.053
1.567MetSer: 1.567 ± 0.051
1.146MetThr: 1.146 ± 0.04
2.02MetVal: 2.02 ± 0.055
0.197MetTrp: 0.197 ± 0.017
0.761MetTyr: 0.761 ± 0.033
0.0MetXaa: 0.0 ± 0.0
Asn
2.586AsnAla: 2.586 ± 0.062
0.425AsnCys: 0.425 ± 0.028
1.798AsnAsp: 1.798 ± 0.055
2.504AsnGlu: 2.504 ± 0.063
1.991AsnPhe: 1.991 ± 0.049
2.313AsnGly: 2.313 ± 0.057
0.57AsnHis: 0.57 ± 0.028
2.213AsnIle: 2.213 ± 0.063
1.621AsnLys: 1.621 ± 0.043
4.138AsnLeu: 4.138 ± 0.089
0.852AsnMet: 0.852 ± 0.038
1.168AsnAsn: 1.168 ± 0.046
1.941AsnPro: 1.941 ± 0.058
1.013AsnGln: 1.013 ± 0.035
1.767AsnArg: 1.767 ± 0.053
1.743AsnSer: 1.743 ± 0.052
1.437AsnThr: 1.437 ± 0.048
4.11AsnVal: 4.11 ± 0.085
0.401AsnTrp: 0.401 ± 0.027
1.301AsnTyr: 1.301 ± 0.036
0.0AsnXaa: 0.0 ± 0.0
Pro
2.528ProAla: 2.528 ± 0.061
0.252ProCys: 0.252 ± 0.018
2.149ProAsp: 2.149 ± 0.064
2.922ProGlu: 2.922 ± 0.065
2.032ProPhe: 2.032 ± 0.06
2.423ProGly: 2.423 ± 0.072
0.783ProHis: 0.783 ± 0.034
2.796ProIle: 2.796 ± 0.064
2.45ProLys: 2.45 ± 0.056
3.553ProLeu: 3.553 ± 0.073
0.928ProMet: 0.928 ± 0.038
1.647ProAsn: 1.647 ± 0.047
1.301ProPro: 1.301 ± 0.047
1.225ProGln: 1.225 ± 0.044
1.81ProArg: 1.81 ± 0.052
2.486ProSer: 2.486 ± 0.066
2.219ProThr: 2.219 ± 0.054
3.278ProVal: 3.278 ± 0.079
0.503ProTrp: 0.503 ± 0.029
1.495ProTyr: 1.495 ± 0.046
0.0ProXaa: 0.0 ± 0.0
Gln
2.035GlnAla: 2.035 ± 0.061
0.158GlnCys: 0.158 ± 0.016
1.297GlnAsp: 1.297 ± 0.045
2.014GlnGlu: 2.014 ± 0.065
1.24GlnPhe: 1.24 ± 0.042
1.453GlnGly: 1.453 ± 0.049
0.46GlnHis: 0.46 ± 0.024
2.75GlnIle: 2.75 ± 0.072
2.022GlnLys: 2.022 ± 0.056
3.478GlnLeu: 3.478 ± 0.07
0.897GlnMet: 0.897 ± 0.033
1.155GlnAsn: 1.155 ± 0.046
1.013GlnPro: 1.013 ± 0.038
1.159GlnGln: 1.159 ± 0.044
1.84GlnArg: 1.84 ± 0.064
1.422GlnSer: 1.422 ± 0.053
1.48GlnThr: 1.48 ± 0.05
2.106GlnVal: 2.106 ± 0.051
0.267GlnTrp: 0.267 ± 0.022
0.845GlnTyr: 0.845 ± 0.036
0.0GlnXaa: 0.0 ± 0.0
Arg
3.344ArgAla: 3.344 ± 0.067
0.579ArgCys: 0.579 ± 0.034
2.313ArgAsp: 2.313 ± 0.065
4.207ArgGlu: 4.207 ± 0.097
3.077ArgPhe: 3.077 ± 0.072
3.113ArgGly: 3.113 ± 0.074
0.88ArgHis: 0.88 ± 0.035
4.641ArgIle: 4.641 ± 0.084
3.968ArgLys: 3.968 ± 0.075
5.638ArgLeu: 5.638 ± 0.101
1.785ArgMet: 1.785 ± 0.05
2.117ArgAsn: 2.117 ± 0.053
1.794ArgPro: 1.794 ± 0.054
1.537ArgGln: 1.537 ± 0.055
3.204ArgArg: 3.204 ± 0.066
3.137ArgSer: 3.137 ± 0.067
2.734ArgThr: 2.734 ± 0.065
4.227ArgVal: 4.227 ± 0.078
0.622ArgTrp: 0.622 ± 0.029
2.04ArgTyr: 2.04 ± 0.057
0.0ArgXaa: 0.0 ± 0.0
Ser
3.731SerAla: 3.731 ± 0.083
0.618SerCys: 0.618 ± 0.032
2.774SerAsp: 2.774 ± 0.065
3.942SerGlu: 3.942 ± 0.084
3.722SerPhe: 3.722 ± 0.085
4.347SerGly: 4.347 ± 0.086
1.068SerHis: 1.068 ± 0.043
4.147SerIle: 4.147 ± 0.08
3.802SerLys: 3.802 ± 0.081
6.718SerLeu: 6.718 ± 0.102
1.665SerMet: 1.665 ± 0.049
2.065SerAsn: 2.065 ± 0.059
2.191SerPro: 2.191 ± 0.063
1.949SerGln: 1.949 ± 0.062
2.917SerArg: 2.917 ± 0.066
3.938SerSer: 3.938 ± 0.085
2.58SerThr: 2.58 ± 0.064
5.036SerVal: 5.036 ± 0.083
0.749SerTrp: 0.749 ± 0.036
2.103SerTyr: 2.103 ± 0.057
0.0SerXaa: 0.0 ± 0.0
Thr
3.371ThrAla: 3.371 ± 0.078
0.445ThrCys: 0.445 ± 0.023
1.985ThrAsp: 1.985 ± 0.06
2.489ThrGlu: 2.489 ± 0.067
2.423ThrPhe: 2.423 ± 0.062
3.513ThrGly: 3.513 ± 0.076
0.931ThrHis: 0.931 ± 0.033
3.65ThrIle: 3.65 ± 0.086
2.773ThrLys: 2.773 ± 0.064
5.118ThrLeu: 5.118 ± 0.101
1.237ThrMet: 1.237 ± 0.048
1.798ThrAsn: 1.798 ± 0.059
2.053ThrPro: 2.053 ± 0.061
1.27ThrGln: 1.27 ± 0.049
2.113ThrArg: 2.113 ± 0.05
2.835ThrSer: 2.835 ± 0.068
2.305ThrThr: 2.305 ± 0.061
4.296ThrVal: 4.296 ± 0.097
0.463ThrTrp: 0.463 ± 0.028
1.515ThrTyr: 1.515 ± 0.049
0.0ThrXaa: 0.0 ± 0.0
Val
6.23ValAla: 6.23 ± 0.093
1.028ValCys: 1.028 ± 0.048
4.317ValAsp: 4.317 ± 0.092
7.031ValGlu: 7.031 ± 0.113
4.211ValPhe: 4.211 ± 0.079
5.96ValGly: 5.96 ± 0.113
1.407ValHis: 1.407 ± 0.05
4.945ValIle: 4.945 ± 0.08
5.505ValLys: 5.505 ± 0.097
9.85ValLeu: 9.85 ± 0.136
1.992ValMet: 1.992 ± 0.058
2.828ValAsn: 2.828 ± 0.064
3.554ValPro: 3.554 ± 0.07
2.607ValGln: 2.607 ± 0.062
4.703ValArg: 4.703 ± 0.092
5.7ValSer: 5.7 ± 0.099
3.774ValThr: 3.774 ± 0.081
7.813ValVal: 7.813 ± 0.128
0.915ValTrp: 0.915 ± 0.036
2.849ValTyr: 2.849 ± 0.059
0.0ValXaa: 0.0 ± 0.0
Trp
0.697TrpAla: 0.697 ± 0.037
0.097TrpCys: 0.097 ± 0.011
0.531TrpAsp: 0.531 ± 0.029
0.6TrpGlu: 0.6 ± 0.032
0.592TrpPhe: 0.592 ± 0.028
0.658TrpGly: 0.658 ± 0.034
0.198TrpHis: 0.198 ± 0.021
0.828TrpIle: 0.828 ± 0.041
0.736TrpLys: 0.736 ± 0.03
1.203TrpLeu: 1.203 ± 0.049
0.33TrpMet: 0.33 ± 0.025
0.486TrpAsn: 0.486 ± 0.032
0.352TrpPro: 0.352 ± 0.024
0.389TrpGln: 0.389 ± 0.025
0.594TrpArg: 0.594 ± 0.032
0.597TrpSer: 0.597 ± 0.033
0.504TrpThr: 0.504 ± 0.034
0.679TrpVal: 0.679 ± 0.03
0.182TrpTrp: 0.182 ± 0.018
0.424TrpTyr: 0.424 ± 0.031
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.468TyrAla: 2.468 ± 0.067
0.319TyrCys: 0.319 ± 0.022
1.931TyrAsp: 1.931 ± 0.056
2.573TyrGlu: 2.573 ± 0.071
1.713TyrPhe: 1.713 ± 0.053
2.473TyrGly: 2.473 ± 0.067
0.634TyrHis: 0.634 ± 0.031
1.785TyrIle: 1.785 ± 0.054
1.655TyrLys: 1.655 ± 0.047
3.474TyrLeu: 3.474 ± 0.074
0.679TyrMet: 0.679 ± 0.035
1.101TyrAsn: 1.101 ± 0.038
1.409TyrPro: 1.409 ± 0.048
0.807TyrGln: 0.807 ± 0.035
2.07TyrArg: 2.07 ± 0.059
2.028TyrSer: 2.028 ± 0.054
1.64TyrThr: 1.64 ± 0.048
3.098TyrVal: 3.098 ± 0.073
0.401TyrTrp: 0.401 ± 0.029
1.213TyrTyr: 1.213 ± 0.047
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2094 proteins (670145 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski