Corynebacterium sphenisci DSM 44792
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1827 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L7CYY5|A0A1L7CYY5_9CORY ABC transporter OS=Corynebacterium sphenisci DSM 44792 OX=1437874 GN=CSPHI_08825 PE=4 SV=1
MM1 pKa = 7.68 NDD3 pKa = 3.31 GGFSGDD9 pKa = 3.36 ANRR12 pKa = 11.84 WGAPGDD18 pKa = 3.76 PQGPGPADD26 pKa = 3.66 GGAGGPMPEE35 pKa = 4.04 QPAWPAEE42 pKa = 4.06 NPEE45 pKa = 4.61 LTWSADD51 pKa = 3.74 PLPPAAPPPAGGHH64 pKa = 6.16 PPAGGYY70 pKa = 7.68 PPPAGYY76 pKa = 9.39 PGAQPAQTAAWSATPHH92 pKa = 6.38 AAQLGQQPPPTAMMPPMPAGSTPTGGGAGGGMLPGIAVVAALLLLLGGGGWWWLTQDD149 pKa = 4.77 ADD151 pKa = 4.18 PAPIAADD158 pKa = 3.27 AEE160 pKa = 4.34 LAEE163 pKa = 4.72 EE164 pKa = 4.9 GGFTASAEE172 pKa = 4.07 PDD174 pKa = 3.72 GEE176 pKa = 4.59 DD177 pKa = 3.59 GSGAGAEE184 pKa = 4.02 AGATGEE190 pKa = 4.41 STPPPAPSPTSSRR203 pKa = 11.84 STEE206 pKa = 3.73 PEE208 pKa = 3.69 ARR210 pKa = 11.84 SSGCSVDD217 pKa = 4.41 ADD219 pKa = 3.56 AAVIGRR225 pKa = 11.84 AVDD228 pKa = 3.7 EE229 pKa = 4.53 VNGKK233 pKa = 9.23 YY234 pKa = 9.8 RR235 pKa = 11.84 GRR237 pKa = 11.84 WEE239 pKa = 3.74 YY240 pKa = 10.75 RR241 pKa = 11.84 GEE243 pKa = 4.45 SNFDD247 pKa = 3.08 GCHH250 pKa = 6.11 PLTYY254 pKa = 10.45 AVLYY258 pKa = 6.57 EE259 pKa = 4.16 TSGSAGHH266 pKa = 6.34 TMLLMFHH273 pKa = 6.63 GPDD276 pKa = 3.52 YY277 pKa = 11.37 LGVDD281 pKa = 3.22 SNYY284 pKa = 9.48 PQRR287 pKa = 11.84 ANSIRR292 pKa = 11.84 VTDD295 pKa = 4.14 DD296 pKa = 3.22 GFIVEE301 pKa = 4.44 YY302 pKa = 10.5 TDD304 pKa = 5.69 LEE306 pKa = 4.77 DD307 pKa = 5.07 GGDD310 pKa = 3.65 SVEE313 pKa = 4.54 SVEE316 pKa = 4.14 YY317 pKa = 8.99 WWDD320 pKa = 3.34 GSKK323 pKa = 10.24 VAHH326 pKa = 6.77 SGRR329 pKa = 11.84 IPNTGYY335 pKa = 10.86 DD336 pKa = 3.38 ALGG339 pKa = 3.48
Molecular weight: 34.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.783
IPC2_protein 3.973
IPC_protein 3.961
Toseland 3.757
ProMoST 4.101
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.846
Rodwell 3.795
Grimsley 3.668
Solomon 3.935
Lehninger 3.884
Nozaki 4.05
DTASelect 4.253
Thurlkill 3.795
EMBOSS 3.859
Sillero 4.075
Patrickios 1.914
IPC_peptide 3.935
IPC2_peptide 4.062
IPC2.peptide.svr19 3.974
Protein with the highest isoelectric point:
>tr|A0A1L7CVH4|A0A1L7CVH4_9CORY Beta-lactamase OS=Corynebacterium sphenisci DSM 44792 OX=1437874 GN=CSPHI_00795 PE=4 SV=1
MM1 pKa = 7.11 NAVAAVWRR9 pKa = 11.84 RR10 pKa = 11.84 DD11 pKa = 3.36 VLRR14 pKa = 11.84 TLRR17 pKa = 11.84 NKK19 pKa = 10.67 AVMVSALLIPGLFLLAFHH37 pKa = 7.15 AAFSRR42 pKa = 11.84 SAADD46 pKa = 4.24 LGIDD50 pKa = 3.56 YY51 pKa = 10.9 ADD53 pKa = 3.8 YY54 pKa = 11.3 LLPTAMMQAIIFTAGGSALAVARR77 pKa = 11.84 DD78 pKa = 3.89 AEE80 pKa = 4.34 EE81 pKa = 5.45 GIHH84 pKa = 6.33 SRR86 pKa = 11.84 LRR88 pKa = 11.84 TMPAPMWAVAAGRR101 pKa = 11.84 VLADD105 pKa = 4.0 LTRR108 pKa = 11.84 MAWSGGIVLALSLALGARR126 pKa = 11.84 IRR128 pKa = 11.84 GGIGGILVMLLIFALVTAIIAAGVDD153 pKa = 4.11 GLCLLSAKK161 pKa = 9.81 PVSTALLFQSLTIIILMFSTAFVPAEE187 pKa = 4.11 ALPDD191 pKa = 3.56 ALAPVIRR198 pKa = 11.84 HH199 pKa = 5.25 VPVSPILEE207 pKa = 4.4 TIRR210 pKa = 11.84 ARR212 pKa = 11.84 MAGDD216 pKa = 3.28 PAGAAGVEE224 pKa = 3.95 AAAWLVALAGAAGWGFVHH242 pKa = 7.09 ALSRR246 pKa = 11.84 RR247 pKa = 11.84 SHH249 pKa = 5.92 AA250 pKa = 4.17
Molecular weight: 25.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.334
IPC2_protein 9.326
IPC_protein 10.409
Toseland 10.379
ProMoST 10.248
Dawson 10.511
Bjellqvist 10.321
Wikipedia 10.804
Rodwell 10.467
Grimsley 10.584
Solomon 10.701
Lehninger 10.657
Nozaki 10.394
DTASelect 10.306
Thurlkill 10.409
EMBOSS 10.804
Sillero 10.467
Patrickios 10.248
IPC_peptide 10.687
IPC2_peptide 9.516
IPC2.peptide.svr19 8.596
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1827
0
1827
605766
40
3043
331.6
35.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
15.97 ± 0.122
0.725 ± 0.014
6.172 ± 0.054
6.113 ± 0.065
2.667 ± 0.035
10.081 ± 0.055
2.028 ± 0.025
3.937 ± 0.036
1.734 ± 0.047
9.979 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.988 ± 0.023
1.699 ± 0.028
6.031 ± 0.054
2.086 ± 0.027
8.268 ± 0.058
3.889 ± 0.037
5.125 ± 0.033
8.336 ± 0.048
1.369 ± 0.025
1.803 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here