Taibaiella sp. KBW10
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3074 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3N7AAX8|A0A3N7AAX8_9BACT S9 family peptidase OS=Taibaiella sp. KBW10 OX=2153357 GN=DBR32_03435 PE=4 SV=1
MM1 pKa = 7.9 EE2 pKa = 6.33 DD3 pKa = 3.24 NMIHH7 pKa = 5.22 VHH9 pKa = 5.78 VEE11 pKa = 3.85 NADD14 pKa = 3.73 GSVDD18 pKa = 3.55 TMEE21 pKa = 5.4 CPTDD25 pKa = 3.33 MGLSLMEE32 pKa = 3.88 MLKK35 pKa = 10.61 ASEE38 pKa = 4.39 YY39 pKa = 10.21 PVLATCGGMALCATCCVDD57 pKa = 4.3 VLEE60 pKa = 5.22 GLDD63 pKa = 3.58 KK64 pKa = 11.22 LGEE67 pKa = 4.19 ISDD70 pKa = 4.95 DD71 pKa = 3.81 EE72 pKa = 4.55 YY73 pKa = 12.04 AMLDD77 pKa = 3.8 TLPDD81 pKa = 4.06 LLPNSRR87 pKa = 11.84 LACQLRR93 pKa = 11.84 LNPDD97 pKa = 2.8 MHH99 pKa = 6.12 GLKK102 pKa = 10.38 FKK104 pKa = 10.56 IHH106 pKa = 7.19 DD107 pKa = 4.07 PQEE110 pKa = 4.09 GG111 pKa = 3.28
Molecular weight: 12.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.846
IPC2_protein 4.101
IPC_protein 4.05
Toseland 3.859
ProMoST 4.202
Dawson 4.037
Bjellqvist 4.19
Wikipedia 3.961
Rodwell 3.884
Grimsley 3.77
Solomon 4.024
Lehninger 3.986
Nozaki 4.151
DTASelect 4.355
Thurlkill 3.897
EMBOSS 3.973
Sillero 4.177
Patrickios 1.036
IPC_peptide 4.024
IPC2_peptide 4.151
IPC2.peptide.svr19 4.05
Protein with the highest isoelectric point:
>tr|A0A3N7CD51|A0A3N7CD51_9BACT Uncharacterized protein OS=Taibaiella sp. KBW10 OX=2153357 GN=DBR32_14535 PE=4 SV=1
MM1 pKa = 7.65 KK2 pKa = 10.09 KK3 pKa = 9.78 HH4 pKa = 5.87 KK5 pKa = 9.21 HH6 pKa = 4.56 TLLLRR11 pKa = 11.84 LALVVILLMHH21 pKa = 6.97 SIPGIFNNGINDD33 pKa = 4.18 FGNLYY38 pKa = 10.32 LNHH41 pKa = 7.17 AGFAPLGVPLAWAIKK56 pKa = 9.72 LSHH59 pKa = 5.87 VVCALCFLCNRR70 pKa = 11.84 YY71 pKa = 8.13 ITIAGIATIFILITGIFMIHH91 pKa = 6.52 LKK93 pKa = 10.28 EE94 pKa = 3.55 GWYY97 pKa = 10.19 VVGGGRR103 pKa = 11.84 NGIEE107 pKa = 4.12 FNLLLIVVTLSVMFPNGWTKK127 pKa = 10.82 NSGIRR132 pKa = 11.84 RR133 pKa = 11.84 RR134 pKa = 11.84 RR135 pKa = 3.28
Molecular weight: 15.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.33
IPC2_protein 9.692
IPC_protein 10.101
Toseland 10.496
ProMoST 10.335
Dawson 10.628
Bjellqvist 10.306
Wikipedia 10.789
Rodwell 11.038
Grimsley 10.672
Solomon 10.687
Lehninger 10.657
Nozaki 10.511
DTASelect 10.292
Thurlkill 10.496
EMBOSS 10.877
Sillero 10.555
Patrickios 10.804
IPC_peptide 10.687
IPC2_peptide 9.399
IPC2.peptide.svr19 8.536
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3074
0
3074
1064520
25
3339
346.3
38.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.988 ± 0.047
0.94 ± 0.018
4.996 ± 0.033
5.364 ± 0.052
4.653 ± 0.034
6.831 ± 0.043
1.915 ± 0.022
7.283 ± 0.037
6.666 ± 0.051
9.248 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.449 ± 0.021
5.697 ± 0.046
3.851 ± 0.026
4.136 ± 0.03
3.594 ± 0.032
6.276 ± 0.036
6.416 ± 0.073
6.252 ± 0.036
1.125 ± 0.016
4.32 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here