Polaribacter sp. MED152

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Polaribacter; unclassified Polaribacter

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2634 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A2U0Z5|A2U0Z5_9FLAO HI0933-like protein OS=Polaribacter sp. MED152 OX=313598 GN=MED152_04120 PE=4 SV=1
MM1 pKa = 7.56KK2 pKa = 10.35KK3 pKa = 10.11FLSFLVFLFCASSAFSQLSKK23 pKa = 10.85KK24 pKa = 10.16HH25 pKa = 6.39FIPPLTSVSTDD36 pKa = 3.48EE37 pKa = 5.19IGQQYY42 pKa = 9.87FYY44 pKa = 11.02ISTPKK49 pKa = 9.24TNNVSYY55 pKa = 10.68KK56 pKa = 8.62ITPIGNPDD64 pKa = 2.54ITAYY68 pKa = 10.46SGVVSNGNPFVQPILDD84 pKa = 3.45SSGNFEE90 pKa = 5.46DD91 pKa = 4.55FTSNSQLHH99 pKa = 5.21ITNFNVGGIIKK110 pKa = 10.31NKK112 pKa = 9.95GFIIEE117 pKa = 4.36ASDD120 pKa = 3.6VIYY123 pKa = 11.06VSVRR127 pKa = 11.84VISQSTFHH135 pKa = 6.95AAALVSKK142 pKa = 10.65GSSALGNSFRR152 pKa = 11.84IGGFVRR158 pKa = 11.84EE159 pKa = 4.12GSTVNGHH166 pKa = 5.78LTFASLMATQNNTTVNITNLPAGFTQPNGSNVPNDD201 pKa = 2.92IVLNEE206 pKa = 4.12GEE208 pKa = 4.62SYY210 pKa = 10.91VVALSALAGQDD221 pKa = 3.48PKK223 pKa = 11.65DD224 pKa = 4.28LIGGLISSDD233 pKa = 3.03KK234 pKa = 10.56PIVVNSGSVAGSFGNGNGRR253 pKa = 11.84DD254 pKa = 3.63YY255 pKa = 11.87GMDD258 pKa = 3.46QIVGADD264 pKa = 3.72KK265 pKa = 10.85IGTEE269 pKa = 4.37YY270 pKa = 10.78ILVRR274 pKa = 11.84GSGIDD279 pKa = 3.43SNEE282 pKa = 3.54NALIIAHH289 pKa = 6.47EE290 pKa = 4.48NDD292 pKa = 3.41TEE294 pKa = 4.09ISINGASSTTTIDD307 pKa = 2.87AGEE310 pKa = 4.21YY311 pKa = 10.28FVVEE315 pKa = 4.03GNNYY319 pKa = 10.15NNGNMYY325 pKa = 10.41ISTSKK330 pKa = 10.85AAFVYY335 pKa = 10.31QGIAEE340 pKa = 4.44INNQFPDD347 pKa = 3.49ANQGLFFVPPLSCEE361 pKa = 3.92NKK363 pKa = 10.31GDD365 pKa = 3.76VNNIASIDD373 pKa = 3.95RR374 pKa = 11.84IGSSNFVGGVTIVTNTGATVTINDD398 pKa = 3.8ADD400 pKa = 3.41ISTFSPQGPTNVTGNANYY418 pKa = 8.36VTYY421 pKa = 10.48NVSGLSGNIKK431 pKa = 10.25VNSDD435 pKa = 3.21QEE437 pKa = 4.48LYY439 pKa = 10.31CAYY442 pKa = 10.37FNRR445 pKa = 11.84SGAATTGAFYY455 pKa = 10.77SGFPSAPEE463 pKa = 3.71INFNTTVASLGSCIPNVTLQAANTDD488 pKa = 3.81LFDD491 pKa = 3.82SFEE494 pKa = 4.08WQFFNPATSNWEE506 pKa = 4.01QRR508 pKa = 11.84STTADD513 pKa = 3.55YY514 pKa = 11.04KK515 pKa = 10.72PLEE518 pKa = 4.54SEE520 pKa = 3.77PGRR523 pKa = 11.84YY524 pKa = 9.05RR525 pKa = 11.84LIGTINCTGATFNSVEE541 pKa = 4.46IPVSLCPDD549 pKa = 4.38DD550 pKa = 4.95YY551 pKa = 11.88DD552 pKa = 5.27SDD554 pKa = 5.78LIIDD558 pKa = 4.27NLDD561 pKa = 3.04VDD563 pKa = 5.12LDD565 pKa = 3.77NDD567 pKa = 4.83GILNSDD573 pKa = 3.7EE574 pKa = 4.33SLGDD578 pKa = 3.37GTINLTNLSNPTVEE592 pKa = 4.57ADD594 pKa = 3.55GAIIPNLLTGNLTSDD609 pKa = 3.77GNVSFVGSTNGNFTSTINDD628 pKa = 3.13NSTSNAANIYY638 pKa = 9.21VLNSSEE644 pKa = 4.05TFNFEE649 pKa = 3.74FTQDD653 pKa = 2.72ASQPHH658 pKa = 5.6TSISGVEE665 pKa = 4.0YY666 pKa = 10.43EE667 pKa = 4.62VGIGPAAKK675 pKa = 9.9NVTLIDD681 pKa = 4.14PDD683 pKa = 4.26NILLVDD689 pKa = 3.75TNFDD693 pKa = 3.81GEE695 pKa = 4.39FEE697 pKa = 4.55AGVDD701 pKa = 3.8NYY703 pKa = 10.99SSSLIRR709 pKa = 11.84FTYY712 pKa = 10.47NPAPNGNTPFKK723 pKa = 10.59FVANDD728 pKa = 2.9VDD730 pKa = 4.22EE731 pKa = 5.54VIFLHH736 pKa = 6.1TATSNVTNSIFNGNIKK752 pKa = 8.79LTNVRR757 pKa = 11.84MDD759 pKa = 3.27SDD761 pKa = 3.89GDD763 pKa = 4.18GIEE766 pKa = 4.9DD767 pKa = 5.07ALDD770 pKa = 4.05LDD772 pKa = 4.3SDD774 pKa = 4.08NDD776 pKa = 3.92GVPDD780 pKa = 3.37IFEE783 pKa = 4.51AANEE787 pKa = 4.47SISLLNTDD795 pKa = 4.49ANLDD799 pKa = 3.73GLDD802 pKa = 4.02DD803 pKa = 4.09VFDD806 pKa = 4.23SVTLVNDD813 pKa = 3.52TDD815 pKa = 4.35GDD817 pKa = 4.27GVPNHH822 pKa = 7.19LDD824 pKa = 3.53LDD826 pKa = 4.04SDD828 pKa = 3.75NDD830 pKa = 4.53GIYY833 pKa = 10.95DD834 pKa = 3.77LVEE837 pKa = 4.22SGFTLTDD844 pKa = 3.43ANNDD848 pKa = 4.2GIIDD852 pKa = 3.73NANAANVGINGLLDD866 pKa = 4.06ALEE869 pKa = 4.32STPDD873 pKa = 3.31SGILANALRR882 pKa = 11.84NSDD885 pKa = 3.25ATSVVTANQDD895 pKa = 3.68TIYY898 pKa = 11.29DD899 pKa = 4.01FADD902 pKa = 3.75LDD904 pKa = 4.67ADD906 pKa = 4.26GDD908 pKa = 4.1DD909 pKa = 4.12CFDD912 pKa = 3.86VIEE915 pKa = 5.3AGFTGNGSGILFANPFATDD934 pKa = 3.34ANGLVINNTDD944 pKa = 3.74GYY946 pKa = 6.23TTPNADD952 pKa = 4.54YY953 pKa = 8.9ITSAPIIVNSFVDD966 pKa = 4.15PVFCEE971 pKa = 4.04LNTEE975 pKa = 4.16VMTIDD980 pKa = 3.56SNADD984 pKa = 2.9AFQWQVSTDD993 pKa = 3.49GTNWTSLTDD1002 pKa = 3.65DD1003 pKa = 3.2ATYY1006 pKa = 11.29NGVTTKK1012 pKa = 10.67DD1013 pKa = 3.49LQITDD1018 pKa = 3.85TPLSFNNNQYY1028 pKa = 10.53RR1029 pKa = 11.84VLLSRR1034 pKa = 11.84TGNSCTEE1041 pKa = 4.21EE1042 pKa = 4.13EE1043 pKa = 4.52SSSVTLTVNPLPIIKK1058 pKa = 10.3NNPSEE1063 pKa = 4.1INQCIDD1069 pKa = 3.78ASDD1072 pKa = 3.76TNPTVNLTTSEE1083 pKa = 4.53DD1084 pKa = 4.11NISDD1088 pKa = 3.77TPNITFEE1095 pKa = 4.23YY1096 pKa = 8.8FTDD1099 pKa = 3.49INGTNQITNPTSYY1112 pKa = 10.17PVTVNTIEE1120 pKa = 4.03VVYY1123 pKa = 10.64VSVISDD1129 pKa = 3.65EE1130 pKa = 4.5GCSNGLVEE1138 pKa = 5.36LRR1140 pKa = 11.84INVGQTPDD1148 pKa = 3.21NPYY1151 pKa = 11.1NDD1153 pKa = 3.98IQPPVCDD1160 pKa = 5.08DD1161 pKa = 4.85LLDD1164 pKa = 4.7ADD1166 pKa = 5.41GNDD1169 pKa = 3.65TPGANDD1175 pKa = 3.57DD1176 pKa = 4.04TDD1178 pKa = 4.87FITNFSLDD1186 pKa = 2.84RR1187 pKa = 11.84DD1188 pKa = 4.26AIVNSINPPANTEE1201 pKa = 3.86VFFYY1205 pKa = 10.89EE1206 pKa = 4.52NSSDD1210 pKa = 3.72RR1211 pKa = 11.84NNSLNEE1217 pKa = 3.61IDD1219 pKa = 3.12ITNYY1223 pKa = 10.28RR1224 pKa = 11.84NDD1226 pKa = 3.29INKK1229 pKa = 9.28IDD1231 pKa = 3.65ITTIPEE1237 pKa = 5.2GIQFPIYY1244 pKa = 10.15YY1245 pKa = 10.01KK1246 pKa = 10.46IISTINNNCQGLGEE1260 pKa = 4.67FFLQIHH1266 pKa = 6.72AVPQATTVSDD1276 pKa = 4.01IEE1278 pKa = 4.32EE1279 pKa = 4.57CDD1281 pKa = 3.73DD1282 pKa = 4.07ALSGNTADD1290 pKa = 5.06GRR1292 pKa = 11.84NAAINLRR1299 pKa = 11.84DD1300 pKa = 3.69KK1301 pKa = 10.88VAEE1304 pKa = 3.96ILGTGQTEE1312 pKa = 3.75ADD1314 pKa = 3.89YY1315 pKa = 11.09EE1316 pKa = 4.89VTFHH1320 pKa = 5.4TTEE1323 pKa = 3.65EE1324 pKa = 4.28GAINNIDD1331 pKa = 4.37VITNDD1336 pKa = 3.32TNYY1339 pKa = 10.33TNQAPSGFTAGTTSEE1354 pKa = 3.63QTIYY1358 pKa = 11.06VRR1360 pKa = 11.84VQNRR1364 pKa = 11.84TGSMCYY1370 pKa = 9.78NANTSFKK1377 pKa = 10.45IIIQPIPTVPANVPDD1392 pKa = 4.82LMVCDD1397 pKa = 4.03VVTPSDD1403 pKa = 3.67ADD1405 pKa = 3.33PRR1407 pKa = 11.84NRR1409 pKa = 11.84IAQNINLTEE1418 pKa = 4.15RR1419 pKa = 11.84EE1420 pKa = 3.74ADD1422 pKa = 3.3ILDD1425 pKa = 3.74GRR1427 pKa = 11.84TGLIVEE1433 pKa = 4.76YY1434 pKa = 10.66YY1435 pKa = 10.15ISQQDD1440 pKa = 3.28AEE1442 pKa = 4.29NRR1444 pKa = 11.84TNAIADD1450 pKa = 3.47PSNFQNTTAEE1460 pKa = 4.31TSFPTDD1466 pKa = 3.83FNTDD1470 pKa = 3.46DD1471 pKa = 5.07PGVQTIFFIIVDD1483 pKa = 4.15EE1484 pKa = 5.08NGLQCPSVFSTFQLLVYY1501 pKa = 8.79PEE1503 pKa = 4.32PSINPVSVLSEE1514 pKa = 4.39CDD1516 pKa = 3.36NDD1518 pKa = 5.72DD1519 pKa = 5.75DD1520 pKa = 6.92GDD1522 pKa = 4.05DD1523 pKa = 3.94ANGIIQTIDD1532 pKa = 3.44LSSKK1536 pKa = 9.71IPEE1539 pKa = 3.93VLGASRR1545 pKa = 11.84NVSDD1549 pKa = 4.82FNVTFHH1555 pKa = 7.28ISQADD1560 pKa = 3.49ATSGDD1565 pKa = 3.47NSLASPYY1572 pKa = 9.65TNSNSTEE1579 pKa = 3.97TIYY1582 pKa = 10.61IRR1584 pKa = 11.84VKK1586 pKa = 10.58NKK1588 pKa = 7.58QTMCVNDD1595 pKa = 3.95DD1596 pKa = 3.94ANFEE1600 pKa = 5.04LIVNPLPNFTVTTPQILCLNDD1621 pKa = 3.28LPYY1624 pKa = 10.79NISVEE1629 pKa = 4.25NPSDD1633 pKa = 3.2NYY1635 pKa = 10.09TYY1637 pKa = 11.41VWTDD1641 pKa = 3.16EE1642 pKa = 4.37NGNTLNPNASVDD1654 pKa = 3.96NIDD1657 pKa = 3.61ISSGGNYY1664 pKa = 9.49KK1665 pKa = 8.53VTATTTDD1672 pKa = 2.71GTMCSRR1678 pKa = 11.84EE1679 pKa = 3.82EE1680 pKa = 4.1TIEE1683 pKa = 4.12VIEE1686 pKa = 4.44SNIATLEE1693 pKa = 4.06SSFITIVDD1701 pKa = 3.39EE1702 pKa = 4.5SNNLGSTNNLSISIDD1717 pKa = 3.75TINNDD1722 pKa = 4.53LGPGDD1727 pKa = 3.84YY1728 pKa = 10.69QFAIVNTDD1736 pKa = 2.66NNTRR1740 pKa = 11.84IPFIGYY1746 pKa = 8.49QDD1748 pKa = 3.58EE1749 pKa = 4.36PLFEE1753 pKa = 4.34NLEE1756 pKa = 4.06GGIYY1760 pKa = 9.47QVIVNDD1766 pKa = 4.77KK1767 pKa = 10.06NGCSPDD1773 pKa = 3.3TTLLVSVIQFPKK1785 pKa = 10.42FFTPNGDD1792 pKa = 3.97NINDD1796 pKa = 3.35TWVVKK1801 pKa = 10.13GANQTFYY1808 pKa = 10.51PNASINIFNRR1818 pKa = 11.84FGNLVAQVPIDD1829 pKa = 3.87SNGWNGMYY1837 pKa = 10.04NGKK1840 pKa = 9.23LLPSDD1845 pKa = 4.74DD1846 pKa = 3.13YY1847 pKa = 10.66WYY1849 pKa = 11.35NVTLVPADD1857 pKa = 3.67TTKK1860 pKa = 9.64PTINKK1865 pKa = 9.34KK1866 pKa = 10.95GNFSLLRR1873 pKa = 11.84KK1874 pKa = 9.56

Molecular weight: 201.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A2U400|A2U400_9FLAO O-glycosyl hydrolase family 30 OS=Polaribacter sp. MED152 OX=313598 GN=MED152_11364 PE=3 SV=1
MM1 pKa = 6.77TKK3 pKa = 9.03IWKK6 pKa = 9.02FFQYY10 pKa = 10.36GYY12 pKa = 11.32LMVAFICLVEE22 pKa = 4.61GIVRR26 pKa = 11.84FNTEE30 pKa = 3.26RR31 pKa = 11.84TRR33 pKa = 11.84AYY35 pKa = 10.58LFLGFSIFITLVFFFKK51 pKa = 10.37RR52 pKa = 11.84HH53 pKa = 4.19FRR55 pKa = 11.84KK56 pKa = 10.32KK57 pKa = 9.44IEE59 pKa = 4.07KK60 pKa = 10.29RR61 pKa = 11.84NNQNKK66 pKa = 9.6

Molecular weight: 8.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2634

0

2634

915757

35

7986

347.7

39.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.276 ± 0.041

0.662 ± 0.017

5.636 ± 0.04

6.556 ± 0.062

5.443 ± 0.047

6.12 ± 0.052

1.597 ± 0.027

8.158 ± 0.048

8.358 ± 0.094

9.222 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.006 ± 0.03

6.779 ± 0.064

3.102 ± 0.025

3.272 ± 0.025

3.17 ± 0.039

6.533 ± 0.055

5.938 ± 0.105

6.235 ± 0.041

0.963 ± 0.018

3.974 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski