Amino acid dipepetide frequency for Polaribacter sp. MED152

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.108AlaAla: 4.108 ± 0.096
0.471AlaCys: 0.471 ± 0.025
3.494AlaAsp: 3.494 ± 0.081
3.825AlaGlu: 3.825 ± 0.073
3.257AlaPhe: 3.257 ± 0.054
3.838AlaGly: 3.838 ± 0.081
0.934AlaHis: 0.934 ± 0.03
5.633AlaIle: 5.633 ± 0.076
5.197AlaLys: 5.197 ± 0.099
5.825AlaLeu: 5.825 ± 0.101
1.386AlaMet: 1.386 ± 0.038
4.061AlaAsn: 4.061 ± 0.088
1.707AlaPro: 1.707 ± 0.05
2.219AlaGln: 2.219 ± 0.044
1.816AlaArg: 1.816 ± 0.048
4.198AlaSer: 4.198 ± 0.097
4.016AlaThr: 4.016 ± 0.141
3.954AlaVal: 3.954 ± 0.072
0.537AlaTrp: 0.537 ± 0.027
2.283AlaTyr: 2.283 ± 0.051
0.0AlaXaa: 0.0 ± 0.0
Cys
0.404CysAla: 0.404 ± 0.025
0.075CysCys: 0.075 ± 0.008
0.378CysAsp: 0.378 ± 0.022
0.404CysGlu: 0.404 ± 0.023
0.361CysPhe: 0.361 ± 0.023
0.523CysGly: 0.523 ± 0.028
0.156CysHis: 0.156 ± 0.016
0.538CysIle: 0.538 ± 0.024
0.49CysLys: 0.49 ± 0.027
0.567CysLeu: 0.567 ± 0.027
0.108CysMet: 0.108 ± 0.013
0.412CysAsn: 0.412 ± 0.022
0.257CysPro: 0.257 ± 0.02
0.171CysGln: 0.171 ± 0.015
0.161CysArg: 0.161 ± 0.015
0.512CysSer: 0.512 ± 0.027
0.394CysThr: 0.394 ± 0.029
0.406CysVal: 0.406 ± 0.022
0.055CysTrp: 0.055 ± 0.008
0.242CysTyr: 0.242 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
3.995AspAla: 3.995 ± 0.084
0.388AspCys: 0.388 ± 0.029
3.21AspAsp: 3.21 ± 0.064
3.88AspGlu: 3.88 ± 0.076
3.909AspPhe: 3.909 ± 0.08
3.537AspGly: 3.537 ± 0.092
0.765AspHis: 0.765 ± 0.027
4.489AspIle: 4.489 ± 0.086
4.473AspLys: 4.473 ± 0.096
5.546AspLeu: 5.546 ± 0.086
0.913AspMet: 0.913 ± 0.034
3.449AspAsn: 3.449 ± 0.087
1.413AspPro: 1.413 ± 0.056
1.353AspGln: 1.353 ± 0.044
1.711AspArg: 1.711 ± 0.045
3.137AspSer: 3.137 ± 0.084
2.788AspThr: 2.788 ± 0.068
4.043AspVal: 4.043 ± 0.087
0.665AspTrp: 0.665 ± 0.028
2.695AspTyr: 2.695 ± 0.06
0.0AspXaa: 0.0 ± 0.0
Glu
4.146GluAla: 4.146 ± 0.094
0.294GluCys: 0.294 ± 0.02
3.62GluAsp: 3.62 ± 0.081
4.967GluGlu: 4.967 ± 0.11
3.135GluPhe: 3.135 ± 0.064
3.588GluGly: 3.588 ± 0.07
0.992GluHis: 0.992 ± 0.039
6.019GluIle: 6.019 ± 0.104
5.956GluLys: 5.956 ± 0.115
6.068GluLeu: 6.068 ± 0.112
1.485GluMet: 1.485 ± 0.039
5.303GluAsn: 5.303 ± 0.094
1.509GluPro: 1.509 ± 0.049
2.116GluGln: 2.116 ± 0.052
2.264GluArg: 2.264 ± 0.053
3.237GluSer: 3.237 ± 0.063
3.681GluThr: 3.681 ± 0.059
4.332GluVal: 4.332 ± 0.069
0.513GluTrp: 0.513 ± 0.026
2.331GluTyr: 2.331 ± 0.057
0.0GluXaa: 0.0 ± 0.0
Phe
3.06PheAla: 3.06 ± 0.064
0.408PheCys: 0.408 ± 0.023
3.486PheAsp: 3.486 ± 0.067
3.18PheGlu: 3.18 ± 0.065
2.868PhePhe: 2.868 ± 0.068
3.806PheGly: 3.806 ± 0.075
0.785PheHis: 0.785 ± 0.031
4.264PheIle: 4.264 ± 0.086
4.247PheLys: 4.247 ± 0.092
5.018PheLeu: 5.018 ± 0.107
1.068PheMet: 1.068 ± 0.033
3.584PheAsn: 3.584 ± 0.069
1.587PhePro: 1.587 ± 0.046
1.564PheGln: 1.564 ± 0.039
1.587PheArg: 1.587 ± 0.045
4.322PheSer: 4.322 ± 0.091
3.494PheThr: 3.494 ± 0.077
3.188PheVal: 3.188 ± 0.058
0.572PheTrp: 0.572 ± 0.028
2.342PheTyr: 2.342 ± 0.069
0.0PheXaa: 0.0 ± 0.0
Gly
3.977GlyAla: 3.977 ± 0.072
0.499GlyCys: 0.499 ± 0.026
3.503GlyAsp: 3.503 ± 0.106
3.31GlyGlu: 3.31 ± 0.059
3.895GlyPhe: 3.895 ± 0.081
4.389GlyGly: 4.389 ± 0.112
0.958GlyHis: 0.958 ± 0.039
5.153GlyIle: 5.153 ± 0.084
4.919GlyLys: 4.919 ± 0.088
5.53GlyLeu: 5.53 ± 0.097
1.39GlyMet: 1.39 ± 0.04
3.738GlyAsn: 3.738 ± 0.1
1.17GlyPro: 1.17 ± 0.049
1.61GlyGln: 1.61 ± 0.045
1.945GlyArg: 1.945 ± 0.051
3.953GlySer: 3.953 ± 0.102
3.766GlyThr: 3.766 ± 0.116
4.269GlyVal: 4.269 ± 0.075
0.654GlyTrp: 0.654 ± 0.027
2.574GlyTyr: 2.574 ± 0.059
0.0GlyXaa: 0.0 ± 0.0
His
0.832HisAla: 0.832 ± 0.035
0.153HisCys: 0.153 ± 0.013
0.687HisAsp: 0.687 ± 0.027
0.875HisGlu: 0.875 ± 0.034
1.045HisPhe: 1.045 ± 0.036
0.869HisGly: 0.869 ± 0.036
0.451HisHis: 0.451 ± 0.028
1.225HisIle: 1.225 ± 0.038
1.334HisLys: 1.334 ± 0.041
1.658HisLeu: 1.658 ± 0.057
0.272HisMet: 0.272 ± 0.017
0.933HisAsn: 0.933 ± 0.035
0.806HisPro: 0.806 ± 0.037
0.738HisGln: 0.738 ± 0.029
0.601HisArg: 0.601 ± 0.03
0.909HisSer: 0.909 ± 0.03
0.857HisThr: 0.857 ± 0.03
0.832HisVal: 0.832 ± 0.035
0.164HisTrp: 0.164 ± 0.014
0.729HisTyr: 0.729 ± 0.029
0.0HisXaa: 0.0 ± 0.0
Ile
5.796IleAla: 5.796 ± 0.092
0.575IleCys: 0.575 ± 0.024
5.021IleAsp: 5.021 ± 0.097
5.455IleGlu: 5.455 ± 0.088
3.896IlePhe: 3.896 ± 0.082
4.898IleGly: 4.898 ± 0.087
1.322IleHis: 1.322 ± 0.045
6.35IleIle: 6.35 ± 0.109
6.408IleLys: 6.408 ± 0.11
7.362IleLeu: 7.362 ± 0.127
1.296IleMet: 1.296 ± 0.036
5.247IleAsn: 5.247 ± 0.1
3.137IlePro: 3.137 ± 0.062
2.678IleGln: 2.678 ± 0.058
2.443IleArg: 2.443 ± 0.056
5.939IleSer: 5.939 ± 0.088
5.155IleThr: 5.155 ± 0.14
5.021IleVal: 5.021 ± 0.072
0.698IleTrp: 0.698 ± 0.025
2.887IleTyr: 2.887 ± 0.061
0.0IleXaa: 0.0 ± 0.0
Lys
5.049LysAla: 5.049 ± 0.106
0.295LysCys: 0.295 ± 0.021
4.716LysAsp: 4.716 ± 0.089
6.884LysGlu: 6.884 ± 0.132
3.302LysPhe: 3.302 ± 0.066
4.674LysGly: 4.674 ± 0.081
1.353LysHis: 1.353 ± 0.044
6.929LysIle: 6.929 ± 0.104
7.925LysLys: 7.925 ± 0.162
6.881LysLeu: 6.881 ± 0.125
2.076LysMet: 2.076 ± 0.057
6.261LysAsn: 6.261 ± 0.11
2.432LysPro: 2.432 ± 0.065
2.863LysGln: 2.863 ± 0.068
2.935LysArg: 2.935 ± 0.073
5.072LysSer: 5.072 ± 0.096
4.882LysThr: 4.882 ± 0.076
5.105LysVal: 5.105 ± 0.089
0.759LysTrp: 0.759 ± 0.035
3.189LysTyr: 3.189 ± 0.074
0.0LysXaa: 0.0 ± 0.0
Leu
5.759LeuAla: 5.759 ± 0.1
0.575LeuCys: 0.575 ± 0.027
5.218LeuAsp: 5.218 ± 0.075
6.628LeuGlu: 6.628 ± 0.108
5.073LeuPhe: 5.073 ± 0.106
5.777LeuGly: 5.777 ± 0.084
1.461LeuHis: 1.461 ± 0.05
7.066LeuIle: 7.066 ± 0.113
8.106LeuLys: 8.106 ± 0.147
8.375LeuLeu: 8.375 ± 0.14
1.896LeuMet: 1.896 ± 0.056
6.124LeuAsn: 6.124 ± 0.092
3.329LeuPro: 3.329 ± 0.07
3.215LeuGln: 3.215 ± 0.061
2.941LeuArg: 2.941 ± 0.067
6.439LeuSer: 6.439 ± 0.103
5.114LeuThr: 5.114 ± 0.132
5.461LeuVal: 5.461 ± 0.081
0.717LeuTrp: 0.717 ± 0.036
2.943LeuTyr: 2.943 ± 0.073
0.0LeuXaa: 0.0 ± 0.0
Met
1.39MetAla: 1.39 ± 0.048
0.128MetCys: 0.128 ± 0.013
1.02MetAsp: 1.02 ± 0.034
1.022MetGlu: 1.022 ± 0.041
0.827MetPhe: 0.827 ± 0.03
1.168MetGly: 1.168 ± 0.039
0.363MetHis: 0.363 ± 0.021
1.553MetIle: 1.553 ± 0.044
2.123MetLys: 2.123 ± 0.051
1.797MetLeu: 1.797 ± 0.047
0.566MetMet: 0.566 ± 0.025
1.333MetAsn: 1.333 ± 0.043
0.779MetPro: 0.779 ± 0.032
0.85MetGln: 0.85 ± 0.032
0.761MetArg: 0.761 ± 0.033
1.39MetSer: 1.39 ± 0.04
0.953MetThr: 0.953 ± 0.032
1.187MetVal: 1.187 ± 0.033
0.138MetTrp: 0.138 ± 0.012
0.714MetTyr: 0.714 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
4.154AsnAla: 4.154 ± 0.08
0.48AsnCys: 0.48 ± 0.026
3.666AsnAsp: 3.666 ± 0.098
3.974AsnGlu: 3.974 ± 0.078
3.6AsnPhe: 3.6 ± 0.08
4.157AsnGly: 4.157 ± 0.119
1.14AsnHis: 1.14 ± 0.04
5.112AsnIle: 5.112 ± 0.074
5.131AsnLys: 5.131 ± 0.103
6.154AsnLeu: 6.154 ± 0.114
1.196AsnMet: 1.196 ± 0.042
4.788AsnAsn: 4.788 ± 0.127
2.746AsnPro: 2.746 ± 0.066
2.573AsnGln: 2.573 ± 0.056
2.133AsnArg: 2.133 ± 0.05
4.572AsnSer: 4.572 ± 0.103
4.183AsnThr: 4.183 ± 0.12
3.996AsnVal: 3.996 ± 0.1
0.823AsnTrp: 0.823 ± 0.033
3.212AsnTyr: 3.212 ± 0.07
0.0AsnXaa: 0.0 ± 0.0
Pro
1.734ProAla: 1.734 ± 0.063
0.171ProCys: 0.171 ± 0.013
1.729ProAsp: 1.729 ± 0.049
2.429ProGlu: 2.429 ± 0.052
1.814ProPhe: 1.814 ± 0.046
1.576ProGly: 1.576 ± 0.046
0.538ProHis: 0.538 ± 0.029
2.688ProIle: 2.688 ± 0.06
2.734ProLys: 2.734 ± 0.069
2.674ProLeu: 2.674 ± 0.061
0.593ProMet: 0.593 ± 0.028
2.346ProAsn: 2.346 ± 0.09
0.639ProPro: 0.639 ± 0.031
0.874ProGln: 0.874 ± 0.027
0.842ProArg: 0.842 ± 0.031
2.001ProSer: 2.001 ± 0.052
2.037ProThr: 2.037 ± 0.068
2.038ProVal: 2.038 ± 0.052
0.328ProTrp: 0.328 ± 0.021
1.237ProTyr: 1.237 ± 0.038
0.0ProXaa: 0.0 ± 0.0
Gln
1.754GlnAla: 1.754 ± 0.044
0.152GlnCys: 0.152 ± 0.012
1.592GlnAsp: 1.592 ± 0.042
2.476GlnGlu: 2.476 ± 0.066
1.803GlnPhe: 1.803 ± 0.045
1.61GlnGly: 1.61 ± 0.046
0.507GlnHis: 0.507 ± 0.024
2.719GlnIle: 2.719 ± 0.051
3.119GlnLys: 3.119 ± 0.07
3.4GlnLeu: 3.4 ± 0.063
0.729GlnMet: 0.729 ± 0.03
2.31GlnAsn: 2.31 ± 0.06
0.972GlnPro: 0.972 ± 0.034
1.443GlnGln: 1.443 ± 0.049
1.152GlnArg: 1.152 ± 0.04
1.759GlnSer: 1.759 ± 0.042
1.896GlnThr: 1.896 ± 0.059
1.859GlnVal: 1.859 ± 0.048
0.282GlnTrp: 0.282 ± 0.019
1.191GlnTyr: 1.191 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
2.007ArgAla: 2.007 ± 0.052
0.159ArgCys: 0.159 ± 0.015
1.589ArgAsp: 1.589 ± 0.047
2.034ArgGlu: 2.034 ± 0.053
1.839ArgPhe: 1.839 ± 0.046
1.827ArgGly: 1.827 ± 0.051
0.484ArgHis: 0.484 ± 0.024
2.754ArgIle: 2.754 ± 0.069
2.874ArgLys: 2.874 ± 0.067
2.877ArgLeu: 2.877 ± 0.066
0.781ArgMet: 0.781 ± 0.032
2.212ArgAsn: 2.212 ± 0.061
0.927ArgPro: 0.927 ± 0.03
0.952ArgGln: 0.952 ± 0.04
1.232ArgArg: 1.232 ± 0.046
1.677ArgSer: 1.677 ± 0.044
1.785ArgThr: 1.785 ± 0.048
2.004ArgVal: 2.004 ± 0.047
0.308ArgTrp: 0.308 ± 0.019
1.373ArgTyr: 1.373 ± 0.047
0.0ArgXaa: 0.0 ± 0.0
Ser
3.755SerAla: 3.755 ± 0.098
0.574SerCys: 0.574 ± 0.035
3.622SerAsp: 3.622 ± 0.079
4.143SerGlu: 4.143 ± 0.069
4.119SerPhe: 4.119 ± 0.073
4.395SerGly: 4.395 ± 0.118
0.954SerHis: 0.954 ± 0.032
5.427SerIle: 5.427 ± 0.095
5.394SerLys: 5.394 ± 0.085
6.121SerLeu: 6.121 ± 0.089
1.245SerMet: 1.245 ± 0.034
4.231SerAsn: 4.231 ± 0.099
1.794SerPro: 1.794 ± 0.051
1.93SerGln: 1.93 ± 0.051
1.886SerArg: 1.886 ± 0.049
4.504SerSer: 4.504 ± 0.099
3.734SerThr: 3.734 ± 0.097
3.975SerVal: 3.975 ± 0.069
0.692SerTrp: 0.692 ± 0.031
2.833SerTyr: 2.833 ± 0.057
0.0SerXaa: 0.0 ± 0.0
Thr
3.687ThrAla: 3.687 ± 0.119
0.364ThrCys: 0.364 ± 0.025
3.313ThrAsp: 3.313 ± 0.114
3.64ThrGlu: 3.64 ± 0.068
3.228ThrPhe: 3.228 ± 0.071
3.712ThrGly: 3.712 ± 0.098
0.91ThrHis: 0.91 ± 0.035
5.414ThrIle: 5.414 ± 0.167
4.442ThrLys: 4.442 ± 0.073
5.429ThrLeu: 5.429 ± 0.099
0.886ThrMet: 0.886 ± 0.034
3.931ThrAsn: 3.931 ± 0.15
2.278ThrPro: 2.278 ± 0.069
1.773ThrGln: 1.773 ± 0.045
1.629ThrArg: 1.629 ± 0.048
4.048ThrSer: 4.048 ± 0.107
3.794ThrThr: 3.794 ± 0.146
3.837ThrVal: 3.837 ± 0.172
0.574ThrTrp: 0.574 ± 0.031
2.491ThrTyr: 2.491 ± 0.075
0.0ThrXaa: 0.0 ± 0.0
Val
4.265ValAla: 4.265 ± 0.075
0.48ValCys: 0.48 ± 0.024
3.865ValAsp: 3.865 ± 0.077
3.811ValGlu: 3.811 ± 0.068
3.32ValPhe: 3.32 ± 0.062
3.875ValGly: 3.875 ± 0.074
0.972ValHis: 0.972 ± 0.033
4.935ValIle: 4.935 ± 0.082
4.619ValLys: 4.619 ± 0.079
6.192ValLeu: 6.192 ± 0.094
1.189ValMet: 1.189 ± 0.033
4.009ValAsn: 4.009 ± 0.093
1.93ValPro: 1.93 ± 0.048
1.753ValGln: 1.753 ± 0.04
1.873ValArg: 1.873 ± 0.049
4.454ValSer: 4.454 ± 0.09
3.817ValThr: 3.817 ± 0.158
4.117ValVal: 4.117 ± 0.074
0.592ValTrp: 0.592 ± 0.029
2.284ValTyr: 2.284 ± 0.05
0.0ValXaa: 0.0 ± 0.0
Trp
0.534TrpAla: 0.534 ± 0.025
0.073TrpCys: 0.073 ± 0.008
0.499TrpAsp: 0.499 ± 0.024
0.55TrpGlu: 0.55 ± 0.026
0.606TrpPhe: 0.606 ± 0.028
0.618TrpGly: 0.618 ± 0.03
0.18TrpHis: 0.18 ± 0.015
0.668TrpIle: 0.668 ± 0.027
0.752TrpLys: 0.752 ± 0.032
0.914TrpLeu: 0.914 ± 0.032
0.302TrpMet: 0.302 ± 0.016
0.686TrpAsn: 0.686 ± 0.031
0.19TrpPro: 0.19 ± 0.017
0.42TrpGln: 0.42 ± 0.02
0.378TrpArg: 0.378 ± 0.024
0.626TrpSer: 0.626 ± 0.032
0.521TrpThr: 0.521 ± 0.044
0.55TrpVal: 0.55 ± 0.029
0.127TrpTrp: 0.127 ± 0.012
0.437TrpTyr: 0.437 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.352TyrAla: 2.352 ± 0.058
0.299TyrCys: 0.299 ± 0.019
2.132TyrAsp: 2.132 ± 0.047
2.07TyrGlu: 2.07 ± 0.059
2.53TyrPhe: 2.53 ± 0.058
2.364TyrGly: 2.364 ± 0.06
0.745TyrHis: 0.745 ± 0.033
2.648TyrIle: 2.648 ± 0.063
3.338TyrLys: 3.338 ± 0.074
3.83TyrLeu: 3.83 ± 0.076
0.684TyrMet: 0.684 ± 0.029
2.834TyrAsn: 2.834 ± 0.071
1.42TyrPro: 1.42 ± 0.044
1.629TyrGln: 1.629 ± 0.047
1.409TyrArg: 1.409 ± 0.043
2.579TyrSer: 2.579 ± 0.056
2.512TyrThr: 2.512 ± 0.078
2.18TyrVal: 2.18 ± 0.041
0.435TyrTrp: 0.435 ± 0.024
1.755TyrTyr: 1.755 ± 0.059
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2634 proteins (915757 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski