Sulfolobus islandicus rudivirus 3

Taxonomy: Viruses; Adnaviria; Zilligvirae; Taleaviricota; Tokiviricetes; Ligamenvirales; Rudiviridae; Rudivirus; unclassified Rudivirus

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B3SN07|A0A1B3SN07_9VIRU Uncharacterized protein OS=Sulfolobus islandicus rudivirus 3 OX=1895333 PE=4 SV=1
MM1 pKa = 7.56NYY3 pKa = 10.03KK4 pKa = 10.02EE5 pKa = 4.48LEE7 pKa = 4.08KK8 pKa = 10.25MLDD11 pKa = 3.59VIFEE15 pKa = 3.9NSEE18 pKa = 3.62IKK20 pKa = 10.61EE21 pKa = 3.61IDD23 pKa = 3.69LFFDD27 pKa = 3.82PEE29 pKa = 4.28VEE31 pKa = 3.71ISKK34 pKa = 10.4QEE36 pKa = 4.1FEE38 pKa = 5.19DD39 pKa = 3.92LVKK42 pKa = 10.98NADD45 pKa = 3.81PLQKK49 pKa = 10.57VVGDD53 pKa = 3.61NYY55 pKa = 9.65ITEE58 pKa = 4.23TFEE61 pKa = 3.21WWEE64 pKa = 3.85FEE66 pKa = 3.96NQYY69 pKa = 11.43LEE71 pKa = 4.86FEE73 pKa = 4.13LDD75 pKa = 3.73YY76 pKa = 11.02YY77 pKa = 11.64VKK79 pKa = 10.55DD80 pKa = 3.46EE81 pKa = 4.75KK82 pKa = 11.1IFVLEE87 pKa = 3.49MHH89 pKa = 7.23FWRR92 pKa = 11.84KK93 pKa = 8.18IRR95 pKa = 11.84KK96 pKa = 8.58

Molecular weight: 12.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B3SN30|A0A1B3SN30_9VIRU Coiled-coil structural protein OS=Sulfolobus islandicus rudivirus 3 OX=1895333 PE=4 SV=1
MM1 pKa = 7.66AKK3 pKa = 10.14GRR5 pKa = 11.84TPRR8 pKa = 11.84SYY10 pKa = 10.31SQRR13 pKa = 11.84YY14 pKa = 8.77AKK16 pKa = 9.27WQAKK20 pKa = 9.13FNAFSNPTVASTILSNVAPVAQQNFQTNVPKK51 pKa = 9.88FTAINEE57 pKa = 4.19NVSAVLSQYY66 pKa = 11.11GITGPNRR73 pKa = 11.84AVYY76 pKa = 10.1QGFALKK82 pKa = 10.23IARR85 pKa = 11.84ALNRR89 pKa = 11.84IGGGPALVNMISGLKK104 pKa = 9.85AYY106 pKa = 9.95YY107 pKa = 9.9ISAFNANPQVLDD119 pKa = 3.52AVVNIITGSPTGYY132 pKa = 10.61VSS134 pKa = 3.22

Molecular weight: 14.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

8767

48

1070

194.8

22.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.118 ± 0.364

0.753 ± 0.161

4.483 ± 0.33

6.821 ± 0.676

6.023 ± 0.302

4.026 ± 0.278

1.049 ± 0.176

10.711 ± 0.455

8.247 ± 0.605

9.798 ± 0.504

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.825 ± 0.192

7.289 ± 0.431

3.148 ± 0.267

4.631 ± 0.477

3.057 ± 0.287

6.536 ± 0.489

5.133 ± 0.372

5.327 ± 0.302

0.867 ± 0.125

6.159 ± 0.323
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski