Lactobacillus phage Bassarid

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Coetzeevirus; unclassified Coetzeevirus

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K9VBZ2|A0A2K9VBZ2_9CAUD Uncharacterized protein OS=Lactobacillus phage Bassarid OX=2079430 PE=4 SV=1
MM1 pKa = 7.49SGGAQRR7 pKa = 11.84VLDD10 pKa = 4.17ALIGYY15 pKa = 9.53DD16 pKa = 4.1KK17 pKa = 11.16EE18 pKa = 4.7CGLEE22 pKa = 4.12FPIQAIIGFFDD33 pKa = 3.83GNIGEE38 pKa = 4.83DD39 pKa = 4.11YY40 pKa = 10.26EE41 pKa = 4.66ALTAEE46 pKa = 4.79EE47 pKa = 4.28EE48 pKa = 4.56SQVIEE53 pKa = 4.23AFLEE57 pKa = 4.0FAYY60 pKa = 10.51

Molecular weight: 6.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K9VC10|A0A2K9VC10_9CAUD Uncharacterized protein OS=Lactobacillus phage Bassarid OX=2079430 PE=4 SV=1
MM1 pKa = 6.88WWFIKK6 pKa = 10.1FALVISFIIGMAIAALPYY24 pKa = 10.32LIALAAVITLIVLLIKK40 pKa = 10.2LLKK43 pKa = 10.01RR44 pKa = 11.84KK45 pKa = 9.67GVII48 pKa = 3.35

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

11589

29

1078

199.8

22.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.242 ± 1.043

0.423 ± 0.103

6.791 ± 0.518

5.954 ± 0.478

3.805 ± 0.228

7.291 ± 0.534

1.493 ± 0.141

6.075 ± 0.222

6.894 ± 0.381

7.783 ± 0.321

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.046 ± 0.204

5.419 ± 0.245

2.934 ± 0.191

3.357 ± 0.17

3.848 ± 0.328

6.498 ± 0.468

6.61 ± 0.238

7.041 ± 0.255

1.104 ± 0.129

4.392 ± 0.428
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski