Amino acid dipepetide frequency for Lactobacillus phage Bassarid

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.269AlaAla: 10.269 ± 3.128
0.259AlaCys: 0.259 ± 0.156
5.005AlaAsp: 5.005 ± 0.779
5.178AlaGlu: 5.178 ± 0.726
3.193AlaPhe: 3.193 ± 0.525
7.335AlaGly: 7.335 ± 1.928
1.381AlaHis: 1.381 ± 0.275
5.609AlaIle: 5.609 ± 1.025
5.609AlaLys: 5.609 ± 0.792
7.68AlaLeu: 7.68 ± 0.859
4.401AlaMet: 4.401 ± 0.801
5.437AlaAsn: 5.437 ± 0.675
2.071AlaPro: 2.071 ± 0.504
3.02AlaGln: 3.02 ± 0.686
3.366AlaArg: 3.366 ± 0.504
5.782AlaSer: 5.782 ± 1.283
5.696AlaThr: 5.696 ± 0.971
6.99AlaVal: 6.99 ± 0.761
1.036AlaTrp: 1.036 ± 0.324
3.107AlaTyr: 3.107 ± 0.542
0.0AlaXaa: 0.0 ± 0.0
Cys
0.259CysAla: 0.259 ± 0.146
0.173CysCys: 0.173 ± 0.148
0.173CysAsp: 0.173 ± 0.145
0.431CysGlu: 0.431 ± 0.217
0.0CysPhe: 0.0 ± 0.0
0.345CysGly: 0.345 ± 0.184
0.173CysHis: 0.173 ± 0.186
0.259CysIle: 0.259 ± 0.178
0.086CysLys: 0.086 ± 0.094
0.0CysLeu: 0.0 ± 0.0
0.259CysMet: 0.259 ± 0.159
0.259CysAsn: 0.259 ± 0.133
0.173CysPro: 0.173 ± 0.144
0.086CysGln: 0.086 ± 0.082
0.345CysArg: 0.345 ± 0.166
0.345CysSer: 0.345 ± 0.203
0.173CysThr: 0.173 ± 0.116
0.431CysVal: 0.431 ± 0.184
0.0CysTrp: 0.0 ± 0.0
0.259CysTyr: 0.259 ± 0.158
0.0CysXaa: 0.0 ± 0.0
Asp
5.782AspAla: 5.782 ± 0.725
0.259AspCys: 0.259 ± 0.145
4.833AspAsp: 4.833 ± 0.841
4.056AspGlu: 4.056 ± 0.713
1.812AspPhe: 1.812 ± 0.368
7.853AspGly: 7.853 ± 0.936
0.777AspHis: 0.777 ± 0.237
3.624AspIle: 3.624 ± 0.506
6.213AspLys: 6.213 ± 0.787
5.696AspLeu: 5.696 ± 0.66
2.416AspMet: 2.416 ± 0.668
4.833AspAsn: 4.833 ± 0.685
1.985AspPro: 1.985 ± 0.473
1.553AspGln: 1.553 ± 0.465
1.812AspArg: 1.812 ± 0.371
3.711AspSer: 3.711 ± 0.654
3.02AspThr: 3.02 ± 0.616
3.797AspVal: 3.797 ± 0.663
1.122AspTrp: 1.122 ± 0.418
2.761AspTyr: 2.761 ± 0.551
0.0AspXaa: 0.0 ± 0.0
Glu
4.833GluAla: 4.833 ± 0.772
0.345GluCys: 0.345 ± 0.186
4.487GluAsp: 4.487 ± 0.67
4.746GluGlu: 4.746 ± 0.83
2.416GluPhe: 2.416 ± 0.564
3.107GluGly: 3.107 ± 0.654
1.553GluHis: 1.553 ± 0.429
3.452GluIle: 3.452 ± 0.639
2.761GluLys: 2.761 ± 0.606
5.696GluLeu: 5.696 ± 0.709
1.726GluMet: 1.726 ± 0.366
2.416GluAsn: 2.416 ± 0.485
1.899GluPro: 1.899 ± 0.409
2.589GluGln: 2.589 ± 0.558
2.675GluArg: 2.675 ± 0.59
3.452GluSer: 3.452 ± 0.539
4.056GluThr: 4.056 ± 0.733
3.279GluVal: 3.279 ± 0.547
0.173GluTrp: 0.173 ± 0.132
3.883GluTyr: 3.883 ± 0.741
0.0GluXaa: 0.0 ± 0.0
Phe
2.589PheAla: 2.589 ± 0.419
0.0PheCys: 0.0 ± 0.0
2.675PheAsp: 2.675 ± 0.562
1.899PheGlu: 1.899 ± 0.38
1.294PhePhe: 1.294 ± 0.29
3.711PheGly: 3.711 ± 0.661
0.518PheHis: 0.518 ± 0.201
2.761PheIle: 2.761 ± 0.525
2.761PheLys: 2.761 ± 0.497
2.244PheLeu: 2.244 ± 0.384
1.208PheMet: 1.208 ± 0.307
2.848PheAsn: 2.848 ± 0.496
0.949PhePro: 0.949 ± 0.249
0.777PheGln: 0.777 ± 0.218
1.381PheArg: 1.381 ± 0.298
2.33PheSer: 2.33 ± 0.374
3.624PheThr: 3.624 ± 0.524
2.33PheVal: 2.33 ± 0.49
0.345PheTrp: 0.345 ± 0.198
1.812PheTyr: 1.812 ± 0.384
0.0PheXaa: 0.0 ± 0.0
Gly
5.954GlyAla: 5.954 ± 1.901
0.259GlyCys: 0.259 ± 0.173
5.264GlyAsp: 5.264 ± 0.922
5.091GlyGlu: 5.091 ± 0.765
3.538GlyPhe: 3.538 ± 0.574
4.746GlyGly: 4.746 ± 0.64
0.431GlyHis: 0.431 ± 0.151
4.66GlyIle: 4.66 ± 0.973
5.437GlyLys: 5.437 ± 0.808
5.005GlyLeu: 5.005 ± 0.776
1.899GlyMet: 1.899 ± 0.564
4.229GlyAsn: 4.229 ± 0.585
0.604GlyPro: 0.604 ± 0.273
2.244GlyGln: 2.244 ± 0.479
2.848GlyArg: 2.848 ± 0.487
5.264GlySer: 5.264 ± 0.645
6.041GlyThr: 6.041 ± 0.813
5.696GlyVal: 5.696 ± 0.76
0.431GlyTrp: 0.431 ± 0.187
3.279GlyTyr: 3.279 ± 0.487
0.0GlyXaa: 0.0 ± 0.0
His
1.036HisAla: 1.036 ± 0.238
0.0HisCys: 0.0 ± 0.0
1.64HisAsp: 1.64 ± 0.426
1.036HisGlu: 1.036 ± 0.269
0.345HisPhe: 0.345 ± 0.185
1.208HisGly: 1.208 ± 0.296
0.173HisHis: 0.173 ± 0.118
0.777HisIle: 0.777 ± 0.294
1.208HisLys: 1.208 ± 0.335
0.69HisLeu: 0.69 ± 0.251
0.259HisMet: 0.259 ± 0.139
0.863HisAsn: 0.863 ± 0.274
0.518HisPro: 0.518 ± 0.24
0.518HisGln: 0.518 ± 0.194
0.69HisArg: 0.69 ± 0.235
1.036HisSer: 1.036 ± 0.247
0.69HisThr: 0.69 ± 0.295
1.208HisVal: 1.208 ± 0.344
0.345HisTrp: 0.345 ± 0.175
0.69HisTyr: 0.69 ± 0.25
0.0HisXaa: 0.0 ± 0.0
Ile
6.127IleAla: 6.127 ± 0.713
0.173IleCys: 0.173 ± 0.124
5.178IleAsp: 5.178 ± 0.726
3.97IleGlu: 3.97 ± 0.77
1.985IlePhe: 1.985 ± 0.393
4.056IleGly: 4.056 ± 0.923
0.863IleHis: 0.863 ± 0.285
3.107IleIle: 3.107 ± 0.538
5.091IleLys: 5.091 ± 0.761
2.589IleLeu: 2.589 ± 0.492
2.157IleMet: 2.157 ± 0.371
4.229IleAsn: 4.229 ± 0.558
1.64IlePro: 1.64 ± 0.309
1.381IleGln: 1.381 ± 0.309
1.553IleArg: 1.553 ± 0.321
3.624IleSer: 3.624 ± 0.574
5.35IleThr: 5.35 ± 0.592
5.091IleVal: 5.091 ± 0.623
0.431IleTrp: 0.431 ± 0.171
2.157IleTyr: 2.157 ± 0.425
0.0IleXaa: 0.0 ± 0.0
Lys
7.249LysAla: 7.249 ± 0.696
0.259LysCys: 0.259 ± 0.16
3.624LysAsp: 3.624 ± 0.65
4.142LysGlu: 4.142 ± 0.673
2.848LysPhe: 2.848 ± 0.387
2.848LysGly: 2.848 ± 0.495
1.381LysHis: 1.381 ± 0.33
2.503LysIle: 2.503 ± 0.464
5.35LysLys: 5.35 ± 0.941
7.076LysLeu: 7.076 ± 1.004
2.675LysMet: 2.675 ± 0.652
4.229LysAsn: 4.229 ± 0.549
1.985LysPro: 1.985 ± 0.373
3.279LysGln: 3.279 ± 0.695
3.279LysArg: 3.279 ± 0.657
4.142LysSer: 4.142 ± 0.855
4.401LysThr: 4.401 ± 0.568
3.711LysVal: 3.711 ± 0.575
0.518LysTrp: 0.518 ± 0.214
3.452LysTyr: 3.452 ± 0.735
0.0LysXaa: 0.0 ± 0.0
Leu
6.3LeuAla: 6.3 ± 0.985
0.345LeuCys: 0.345 ± 0.181
5.523LeuAsp: 5.523 ± 0.725
4.229LeuGlu: 4.229 ± 0.842
2.589LeuPhe: 2.589 ± 0.578
4.401LeuGly: 4.401 ± 0.639
1.208LeuHis: 1.208 ± 0.316
5.264LeuIle: 5.264 ± 0.738
6.127LeuLys: 6.127 ± 0.69
6.472LeuLeu: 6.472 ± 0.675
2.071LeuMet: 2.071 ± 0.455
3.97LeuAsn: 3.97 ± 0.53
3.366LeuPro: 3.366 ± 0.465
3.02LeuGln: 3.02 ± 0.554
2.157LeuArg: 2.157 ± 0.416
5.954LeuSer: 5.954 ± 0.864
5.264LeuThr: 5.264 ± 0.742
5.523LeuVal: 5.523 ± 0.699
1.122LeuTrp: 1.122 ± 0.34
2.934LeuTyr: 2.934 ± 0.661
0.0LeuXaa: 0.0 ± 0.0
Met
3.711MetAla: 3.711 ± 0.767
0.259MetCys: 0.259 ± 0.156
1.381MetAsp: 1.381 ± 0.403
1.122MetGlu: 1.122 ± 0.318
1.381MetPhe: 1.381 ± 0.352
1.812MetGly: 1.812 ± 0.531
0.259MetHis: 0.259 ± 0.158
2.157MetIle: 2.157 ± 0.589
1.553MetLys: 1.553 ± 0.33
3.02MetLeu: 3.02 ± 0.636
1.036MetMet: 1.036 ± 0.33
1.122MetAsn: 1.122 ± 0.345
0.431MetPro: 0.431 ± 0.184
1.467MetGln: 1.467 ± 0.435
1.726MetArg: 1.726 ± 0.468
2.071MetSer: 2.071 ± 0.423
2.848MetThr: 2.848 ± 0.504
1.64MetVal: 1.64 ± 0.325
0.086MetTrp: 0.086 ± 0.082
1.294MetTyr: 1.294 ± 0.404
0.0MetXaa: 0.0 ± 0.0
Asn
4.574AsnAla: 4.574 ± 0.6
0.431AsnCys: 0.431 ± 0.213
4.142AsnAsp: 4.142 ± 0.652
3.02AsnGlu: 3.02 ± 0.519
1.467AsnPhe: 1.467 ± 0.432
5.868AsnGly: 5.868 ± 0.694
1.036AsnHis: 1.036 ± 0.285
3.366AsnIle: 3.366 ± 0.467
3.797AsnLys: 3.797 ± 0.632
3.107AsnLeu: 3.107 ± 0.514
1.467AsnMet: 1.467 ± 0.425
3.107AsnAsn: 3.107 ± 0.687
0.949AsnPro: 0.949 ± 0.349
1.381AsnGln: 1.381 ± 0.257
1.985AsnArg: 1.985 ± 0.384
3.366AsnSer: 3.366 ± 0.593
3.452AsnThr: 3.452 ± 0.524
4.315AsnVal: 4.315 ± 0.582
0.949AsnTrp: 0.949 ± 0.283
2.416AsnTyr: 2.416 ± 0.542
0.0AsnXaa: 0.0 ± 0.0
Pro
2.675ProAla: 2.675 ± 0.448
0.173ProCys: 0.173 ± 0.136
2.157ProAsp: 2.157 ± 0.472
2.244ProGlu: 2.244 ± 0.455
1.553ProPhe: 1.553 ± 0.385
1.985ProGly: 1.985 ± 0.447
0.173ProHis: 0.173 ± 0.113
1.64ProIle: 1.64 ± 0.392
1.812ProLys: 1.812 ± 0.437
2.244ProLeu: 2.244 ± 0.431
0.604ProMet: 0.604 ± 0.205
1.467ProAsn: 1.467 ± 0.325
0.345ProPro: 0.345 ± 0.228
1.381ProGln: 1.381 ± 0.344
1.036ProArg: 1.036 ± 0.343
1.899ProSer: 1.899 ± 0.504
2.244ProThr: 2.244 ± 0.509
1.812ProVal: 1.812 ± 0.373
0.259ProTrp: 0.259 ± 0.137
1.64ProTyr: 1.64 ± 0.283
0.0ProXaa: 0.0 ± 0.0
Gln
3.97GlnAla: 3.97 ± 0.647
0.086GlnCys: 0.086 ± 0.085
0.863GlnAsp: 0.863 ± 0.322
2.244GlnGlu: 2.244 ± 0.438
1.208GlnPhe: 1.208 ± 0.354
2.157GlnGly: 2.157 ± 0.449
0.777GlnHis: 0.777 ± 0.211
1.726GlnIle: 1.726 ± 0.327
1.64GlnLys: 1.64 ± 0.436
3.366GlnLeu: 3.366 ± 0.545
1.122GlnMet: 1.122 ± 0.311
1.208GlnAsn: 1.208 ± 0.26
1.294GlnPro: 1.294 ± 0.325
1.208GlnGln: 1.208 ± 0.259
1.294GlnArg: 1.294 ± 0.375
2.589GlnSer: 2.589 ± 0.545
2.589GlnThr: 2.589 ± 0.517
2.848GlnVal: 2.848 ± 0.548
0.173GlnTrp: 0.173 ± 0.107
1.208GlnTyr: 1.208 ± 0.337
0.0GlnXaa: 0.0 ± 0.0
Arg
3.97ArgAla: 3.97 ± 0.499
0.086ArgCys: 0.086 ± 0.094
2.33ArgAsp: 2.33 ± 0.421
2.071ArgGlu: 2.071 ± 0.469
2.589ArgPhe: 2.589 ± 0.563
2.503ArgGly: 2.503 ± 0.342
0.518ArgHis: 0.518 ± 0.24
2.934ArgIle: 2.934 ± 0.499
2.416ArgLys: 2.416 ± 0.488
3.538ArgLeu: 3.538 ± 0.644
0.604ArgMet: 0.604 ± 0.207
1.381ArgAsn: 1.381 ± 0.384
1.381ArgPro: 1.381 ± 0.498
1.036ArgGln: 1.036 ± 0.303
1.899ArgArg: 1.899 ± 0.57
1.64ArgSer: 1.64 ± 0.358
2.244ArgThr: 2.244 ± 0.488
2.761ArgVal: 2.761 ± 0.62
0.69ArgTrp: 0.69 ± 0.225
1.899ArgTyr: 1.899 ± 0.391
0.0ArgXaa: 0.0 ± 0.0
Ser
5.437SerAla: 5.437 ± 1.586
0.173SerCys: 0.173 ± 0.17
5.091SerAsp: 5.091 ± 0.722
3.193SerGlu: 3.193 ± 0.539
2.675SerPhe: 2.675 ± 0.459
5.782SerGly: 5.782 ± 1.007
1.036SerHis: 1.036 ± 0.214
3.797SerIle: 3.797 ± 0.544
3.797SerLys: 3.797 ± 0.535
5.609SerLeu: 5.609 ± 0.679
2.157SerMet: 2.157 ± 0.428
2.675SerAsn: 2.675 ± 0.454
2.244SerPro: 2.244 ± 0.383
2.244SerGln: 2.244 ± 0.376
1.467SerArg: 1.467 ± 0.399
6.127SerSer: 6.127 ± 0.922
3.279SerThr: 3.279 ± 0.463
5.178SerVal: 5.178 ± 0.665
0.863SerTrp: 0.863 ± 0.32
2.157SerTyr: 2.157 ± 0.509
0.0SerXaa: 0.0 ± 0.0
Thr
6.817ThrAla: 6.817 ± 0.877
0.345ThrCys: 0.345 ± 0.17
5.005ThrAsp: 5.005 ± 0.905
3.279ThrGlu: 3.279 ± 0.58
2.761ThrPhe: 2.761 ± 0.46
5.609ThrGly: 5.609 ± 0.75
0.777ThrHis: 0.777 ± 0.252
4.056ThrIle: 4.056 ± 0.692
2.934ThrLys: 2.934 ± 0.397
5.35ThrLeu: 5.35 ± 0.752
1.294ThrMet: 1.294 ± 0.296
2.934ThrAsn: 2.934 ± 0.61
3.02ThrPro: 3.02 ± 0.528
2.416ThrGln: 2.416 ± 0.424
2.675ThrArg: 2.675 ± 0.467
3.279ThrSer: 3.279 ± 0.527
3.538ThrThr: 3.538 ± 0.602
5.523ThrVal: 5.523 ± 0.642
0.863ThrTrp: 0.863 ± 0.248
3.624ThrTyr: 3.624 ± 0.551
0.0ThrXaa: 0.0 ± 0.0
Val
6.817ValAla: 6.817 ± 1.028
0.173ValCys: 0.173 ± 0.147
4.919ValAsp: 4.919 ± 0.66
3.711ValGlu: 3.711 ± 0.523
2.33ValPhe: 2.33 ± 0.492
4.315ValGly: 4.315 ± 0.583
1.208ValHis: 1.208 ± 0.32
5.005ValIle: 5.005 ± 0.508
5.609ValLys: 5.609 ± 0.754
4.487ValLeu: 4.487 ± 0.78
1.467ValMet: 1.467 ± 0.301
3.193ValAsn: 3.193 ± 0.537
2.761ValPro: 2.761 ± 0.506
1.899ValGln: 1.899 ± 0.417
3.97ValArg: 3.97 ± 0.586
4.919ValSer: 4.919 ± 0.72
4.833ValThr: 4.833 ± 0.614
4.746ValVal: 4.746 ± 0.742
1.036ValTrp: 1.036 ± 0.291
3.02ValTyr: 3.02 ± 0.61
0.0ValXaa: 0.0 ± 0.0
Trp
0.863TrpAla: 0.863 ± 0.248
0.0TrpCys: 0.0 ± 0.0
0.863TrpAsp: 0.863 ± 0.266
0.69TrpGlu: 0.69 ± 0.207
0.604TrpPhe: 0.604 ± 0.267
0.345TrpGly: 0.345 ± 0.157
0.259TrpHis: 0.259 ± 0.187
1.036TrpIle: 1.036 ± 0.307
0.69TrpLys: 0.69 ± 0.264
1.208TrpLeu: 1.208 ± 0.423
0.173TrpMet: 0.173 ± 0.125
0.69TrpAsn: 0.69 ± 0.226
0.173TrpPro: 0.173 ± 0.114
0.086TrpGln: 0.086 ± 0.084
0.431TrpArg: 0.431 ± 0.211
1.036TrpSer: 1.036 ± 0.255
0.345TrpThr: 0.345 ± 0.186
0.604TrpVal: 0.604 ± 0.246
0.259TrpTrp: 0.259 ± 0.148
0.69TrpTyr: 0.69 ± 0.286
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.193TyrAla: 3.193 ± 0.624
0.431TyrCys: 0.431 ± 0.177
2.761TyrAsp: 2.761 ± 0.612
3.193TyrGlu: 3.193 ± 0.6
1.467TyrPhe: 1.467 ± 0.363
2.934TyrGly: 2.934 ± 0.412
0.431TyrHis: 0.431 ± 0.263
3.02TyrIle: 3.02 ± 0.463
4.056TyrLys: 4.056 ± 0.897
2.761TyrLeu: 2.761 ± 0.674
1.381TyrMet: 1.381 ± 0.274
3.107TyrAsn: 3.107 ± 0.507
1.553TyrPro: 1.553 ± 0.369
1.985TyrGln: 1.985 ± 0.466
1.899TyrArg: 1.899 ± 0.454
2.416TyrSer: 2.416 ± 0.515
2.416TyrThr: 2.416 ± 0.572
2.934TyrVal: 2.934 ± 0.586
0.345TyrTrp: 0.345 ± 0.155
1.64TyrTyr: 1.64 ± 0.386
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 58 proteins (11589 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski