Staphylococcus auricularis
Average proteome isoelectric point is 5.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2003 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2K4BZE8|A0A2K4BZE8_9STAP Organic hydroperoxide resistance protein OS=Staphylococcus auricularis OX=29379 GN=CD158_05380 PE=3 SV=1
MM1 pKa = 7.28 TNQVIQVDD9 pKa = 4.13 GMSCEE14 pKa = 4.06 HH15 pKa = 6.83 CKK17 pKa = 10.7 SAVEE21 pKa = 4.08 NALTALSGVDD31 pKa = 3.22 QAEE34 pKa = 4.48 VNLEE38 pKa = 4.17 AGNVTVDD45 pKa = 3.38 YY46 pKa = 10.89 DD47 pKa = 4.03 SEE49 pKa = 4.53 KK50 pKa = 10.23 VTTNDD55 pKa = 2.93 MKK57 pKa = 11.1 DD58 pKa = 3.25 AIEE61 pKa = 4.34 DD62 pKa = 3.07 QGYY65 pKa = 10.08 DD66 pKa = 3.55 VPQLL70 pKa = 3.47
Molecular weight: 7.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.761
IPC2_protein 3.77
IPC_protein 3.694
Toseland 3.49
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.414
Solomon 3.656
Lehninger 3.617
Nozaki 3.834
DTASelect 4.012
Thurlkill 3.567
EMBOSS 3.656
Sillero 3.821
Patrickios 1.863
IPC_peptide 3.656
IPC2_peptide 3.783
IPC2.peptide.svr19 3.748
Protein with the highest isoelectric point:
>tr|A0A2K4C221|A0A2K4C221_9STAP Nitrate reductase subunit gamma OS=Staphylococcus auricularis OX=29379 GN=narI PE=4 SV=1
MM1 pKa = 7.49 VKK3 pKa = 9.1 RR4 pKa = 11.84 TYY6 pKa = 10.35 QPNKK10 pKa = 8.16 RR11 pKa = 11.84 KK12 pKa = 9.54 HH13 pKa = 5.99 SKK15 pKa = 8.87 VHH17 pKa = 5.77 GFRR20 pKa = 11.84 KK21 pKa = 10.02 RR22 pKa = 11.84 MSTKK26 pKa = 10.18 NGRR29 pKa = 11.84 KK30 pKa = 8.49 VLARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.05 GRR40 pKa = 11.84 KK41 pKa = 8.7 VLSAA45 pKa = 4.05
Molecular weight: 5.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.415
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.427
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.149
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.006
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2003
0
2003
599734
24
3777
299.4
33.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.814 ± 0.063
0.599 ± 0.015
6.388 ± 0.068
7.047 ± 0.066
4.348 ± 0.049
6.286 ± 0.062
2.455 ± 0.033
7.97 ± 0.065
6.413 ± 0.056
9.285 ± 0.067
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.653 ± 0.03
4.791 ± 0.045
3.416 ± 0.038
4.558 ± 0.056
3.738 ± 0.041
5.871 ± 0.093
5.862 ± 0.041
6.947 ± 0.052
0.745 ± 0.019
3.813 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here