Bifidobacterium samirii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1965 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A430FF14|A0A430FF14_9BIFI DNA topoisomerase (ATP-hydrolyzing) OS=Bifidobacterium samirii OX=2306974 GN=D2E24_1911 PE=4 SV=1
MM1 pKa = 7.73SIHH4 pKa = 7.13DD5 pKa = 3.28HH6 pKa = 5.46HH7 pKa = 7.29QRR9 pKa = 11.84IRR11 pKa = 11.84EE12 pKa = 4.15TVRR15 pKa = 11.84TVSDD19 pKa = 3.52AAAQLARR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84VTAVTALVLALACVAAATLTYY49 pKa = 10.41GVVWPSFDD57 pKa = 5.11DD58 pKa = 4.62GASSAAAGAAEE69 pKa = 4.41APAASSADD77 pKa = 3.42GSSTATAAAQSDD89 pKa = 4.36DD90 pKa = 3.6EE91 pKa = 4.78SAVADD96 pKa = 4.45ASAASPVDD104 pKa = 3.45TSLAALDD111 pKa = 4.38DD112 pKa = 4.6LGGAAAVSTVGFDD125 pKa = 4.97LPDD128 pKa = 3.47EE129 pKa = 4.46SLAALQAEE137 pKa = 4.99LDD139 pKa = 3.78TFASYY144 pKa = 10.75GYY146 pKa = 8.71GASVALVDD154 pKa = 4.0VTTGAAISIDD164 pKa = 3.64GGTARR169 pKa = 11.84YY170 pKa = 8.59SASAIKK176 pKa = 10.68GPFVLSLAASGTIDD190 pKa = 3.86ADD192 pKa = 3.74AAVAAATYY200 pKa = 10.05EE201 pKa = 4.02DD202 pKa = 4.8DD203 pKa = 3.94YY204 pKa = 11.96VRR206 pKa = 11.84QLVEE210 pKa = 3.82ATVTVSDD217 pKa = 3.59NDD219 pKa = 3.49AYY221 pKa = 10.31ATLFEE226 pKa = 5.19TYY228 pKa = 9.11WSDD231 pKa = 5.91AIEE234 pKa = 3.82QWAAEE239 pKa = 4.28SSVTAPIAGAEE250 pKa = 4.05YY251 pKa = 10.91LDD253 pKa = 3.9ISAIDD258 pKa = 3.81MARR261 pKa = 11.84LWTMGYY267 pKa = 10.69GYY269 pKa = 10.97LFADD273 pKa = 4.66DD274 pKa = 5.48ADD276 pKa = 4.49AADD279 pKa = 4.25EE280 pKa = 4.38SADD283 pKa = 3.69GSSDD287 pKa = 3.36AAVVASASDD296 pKa = 3.59GAEE299 pKa = 3.93SGRR302 pKa = 11.84AWLADD307 pKa = 3.23AFSNTLNSSIHH318 pKa = 5.29MALVGGDD325 pKa = 4.1DD326 pKa = 4.6GSDD329 pKa = 2.93ASAAADD335 pKa = 3.59GAAAATSGTTVYY347 pKa = 10.42TKK349 pKa = 10.71AGWINGEE356 pKa = 3.96GGLYY360 pKa = 10.56ALNDD364 pKa = 3.66AGIVRR369 pKa = 11.84SASGDD374 pKa = 3.63YY375 pKa = 10.39ILAVMTDD382 pKa = 2.78ACGEE386 pKa = 4.16YY387 pKa = 11.06GLLTDD392 pKa = 6.58LITLLDD398 pKa = 4.44SIHH401 pKa = 6.77AEE403 pKa = 3.98SMAAA407 pKa = 3.82

Molecular weight: 40.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A430FV92|A0A430FV92_9BIFI Ribosomal RNA small subunit methyltransferase G OS=Bifidobacterium samirii OX=2306974 GN=rsmG PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 4.82MKK15 pKa = 9.64HH16 pKa = 5.7GFRR19 pKa = 11.84LRR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84SGRR28 pKa = 11.84AVINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.06GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1965

0

1965

737665

29

3195

375.4

40.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.283 ± 0.083

0.947 ± 0.019

7.473 ± 0.062

5.441 ± 0.05

3.267 ± 0.035

8.465 ± 0.048

2.067 ± 0.026

4.839 ± 0.041

2.956 ± 0.053

8.585 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.623 ± 0.027

2.716 ± 0.037

4.81 ± 0.045

2.892 ± 0.036

6.909 ± 0.067

5.501 ± 0.044

6.225 ± 0.052

7.812 ± 0.054

1.421 ± 0.022

2.768 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski