Streptococcus phage Javan548

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6B9I7|A0A4D6B9I7_9CAUD Uncharacterized protein OS=Streptococcus phage Javan548 OX=2548251 GN=Javan548_0016 PE=4 SV=1
MM1 pKa = 7.6TKK3 pKa = 9.63TEE5 pKa = 4.16LAPYY9 pKa = 10.05LNSLSSGHH17 pKa = 5.93SVALLFHH24 pKa = 6.6TANGTYY30 pKa = 8.5ITTEE34 pKa = 4.06VNFSDD39 pKa = 3.68GDD41 pKa = 4.08TVVFSYY47 pKa = 10.73EE48 pKa = 3.5QGFASLEE55 pKa = 3.79YY56 pKa = 10.92DD57 pKa = 4.81DD58 pKa = 4.43IQKK61 pKa = 8.13VTALKK66 pKa = 10.35FLEE69 pKa = 4.27SHH71 pKa = 6.71SLDD74 pKa = 3.47TT75 pKa = 5.22

Molecular weight: 8.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6B9Z0|A0A4D6B9Z0_9CAUD Uncharacterized protein OS=Streptococcus phage Javan548 OX=2548251 GN=Javan548_0048 PE=4 SV=1
MM1 pKa = 7.72ASMKK5 pKa = 10.36FKK7 pKa = 10.99LNRR10 pKa = 11.84AGVRR14 pKa = 11.84EE15 pKa = 4.12LMKK18 pKa = 10.61SPEE21 pKa = 4.08MQAVLTDD28 pKa = 3.37KK29 pKa = 11.39ANAIRR34 pKa = 11.84NRR36 pKa = 11.84AGDD39 pKa = 4.0GYY41 pKa = 11.09EE42 pKa = 3.67SDD44 pKa = 3.49IYY46 pKa = 10.89VGKK49 pKa = 8.48TRR51 pKa = 11.84ANAMVYY57 pKa = 10.69ADD59 pKa = 4.22SIKK62 pKa = 10.85AKK64 pKa = 10.25RR65 pKa = 11.84DD66 pKa = 3.58NKK68 pKa = 10.31KK69 pKa = 10.32HH70 pKa = 4.82NTLLKK75 pKa = 10.33AVKK78 pKa = 10.03SS79 pKa = 3.89

Molecular weight: 8.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

9945

39

1226

198.9

22.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.954 ± 0.546

0.593 ± 0.109

6.164 ± 0.287

7.381 ± 0.523

3.962 ± 0.249

6.506 ± 0.553

1.428 ± 0.192

6.637 ± 0.306

7.813 ± 0.55

8.054 ± 0.275

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.594 ± 0.216

5.51 ± 0.261

2.826 ± 0.255

4.183 ± 0.208

4.686 ± 0.368

6.395 ± 0.478

6.033 ± 0.427

6.677 ± 0.274

1.207 ± 0.104

3.399 ± 0.236
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski