Amino acid dipepetide frequency for Streptococcus phage Javan548

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.721AlaAla: 3.721 ± 0.71
0.603AlaCys: 0.603 ± 0.329
4.928AlaAsp: 4.928 ± 0.609
7.643AlaGlu: 7.643 ± 0.81
2.212AlaPhe: 2.212 ± 0.477
6.134AlaGly: 6.134 ± 1.085
1.106AlaHis: 1.106 ± 0.357
6.637AlaIle: 6.637 ± 1.075
7.844AlaLys: 7.844 ± 1.02
6.335AlaLeu: 6.335 ± 0.749
1.81AlaMet: 1.81 ± 0.394
5.632AlaAsn: 5.632 ± 0.75
1.006AlaPro: 1.006 ± 0.317
3.821AlaGln: 3.821 ± 0.827
2.715AlaArg: 2.715 ± 0.556
4.928AlaSer: 4.928 ± 0.818
4.726AlaThr: 4.726 ± 0.911
4.928AlaVal: 4.928 ± 0.694
0.905AlaTrp: 0.905 ± 0.317
1.911AlaTyr: 1.911 ± 0.412
0.0AlaXaa: 0.0 ± 0.0
Cys
0.402CysAla: 0.402 ± 0.248
0.201CysCys: 0.201 ± 0.132
0.402CysAsp: 0.402 ± 0.179
0.402CysGlu: 0.402 ± 0.179
0.201CysPhe: 0.201 ± 0.141
0.402CysGly: 0.402 ± 0.242
0.201CysHis: 0.201 ± 0.137
0.302CysIle: 0.302 ± 0.169
0.302CysLys: 0.302 ± 0.196
0.503CysLeu: 0.503 ± 0.237
0.0CysMet: 0.0 ± 0.0
0.101CysAsn: 0.101 ± 0.107
0.201CysPro: 0.201 ± 0.126
0.201CysGln: 0.201 ± 0.166
0.503CysArg: 0.503 ± 0.345
0.503CysSer: 0.503 ± 0.205
0.101CysThr: 0.101 ± 0.11
0.503CysVal: 0.503 ± 0.265
0.101CysTrp: 0.101 ± 0.109
0.402CysTyr: 0.402 ± 0.223
0.0CysXaa: 0.0 ± 0.0
Asp
3.419AspAla: 3.419 ± 0.615
0.603AspCys: 0.603 ± 0.254
3.52AspAsp: 3.52 ± 0.616
4.224AspGlu: 4.224 ± 0.821
3.218AspPhe: 3.218 ± 0.717
4.827AspGly: 4.827 ± 0.633
0.805AspHis: 0.805 ± 0.285
4.928AspIle: 4.928 ± 0.641
4.425AspLys: 4.425 ± 0.64
5.732AspLeu: 5.732 ± 0.649
1.81AspMet: 1.81 ± 0.508
3.419AspAsn: 3.419 ± 0.534
1.609AspPro: 1.609 ± 0.428
1.307AspGln: 1.307 ± 0.34
3.52AspArg: 3.52 ± 0.619
3.419AspSer: 3.419 ± 0.501
3.017AspThr: 3.017 ± 0.519
4.626AspVal: 4.626 ± 0.684
1.106AspTrp: 1.106 ± 0.283
2.112AspTyr: 2.112 ± 0.624
0.0AspXaa: 0.0 ± 0.0
Glu
6.335GluAla: 6.335 ± 0.851
0.201GluCys: 0.201 ± 0.135
3.218GluAsp: 3.218 ± 0.653
5.129GluGlu: 5.129 ± 0.985
2.414GluPhe: 2.414 ± 0.429
3.922GluGly: 3.922 ± 0.597
1.408GluHis: 1.408 ± 0.46
5.632GluIle: 5.632 ± 0.989
4.626GluLys: 4.626 ± 0.915
7.14GluLeu: 7.14 ± 1.038
3.017GluMet: 3.017 ± 0.678
5.028GluAsn: 5.028 ± 0.719
1.408GluPro: 1.408 ± 0.459
3.117GluGln: 3.117 ± 0.518
4.827GluArg: 4.827 ± 0.793
4.425GluSer: 4.425 ± 0.603
4.023GluThr: 4.023 ± 0.7
4.626GluVal: 4.626 ± 0.731
0.704GluTrp: 0.704 ± 0.229
2.514GluTyr: 2.514 ± 0.711
0.0GluXaa: 0.0 ± 0.0
Phe
3.319PheAla: 3.319 ± 0.496
0.0PheCys: 0.0 ± 0.0
3.62PheAsp: 3.62 ± 0.625
5.43PheGlu: 5.43 ± 0.804
1.408PhePhe: 1.408 ± 0.281
3.62PheGly: 3.62 ± 0.66
0.503PheHis: 0.503 ± 0.198
2.212PheIle: 2.212 ± 0.447
2.514PheLys: 2.514 ± 0.376
2.313PheLeu: 2.313 ± 0.562
1.408PheMet: 1.408 ± 0.338
2.011PheAsn: 2.011 ± 0.5
1.106PhePro: 1.106 ± 0.304
0.603PheGln: 0.603 ± 0.306
1.408PheArg: 1.408 ± 0.501
1.609PheSer: 1.609 ± 0.453
2.313PheThr: 2.313 ± 0.552
2.313PheVal: 2.313 ± 0.463
0.402PheTrp: 0.402 ± 0.222
1.508PheTyr: 1.508 ± 0.34
0.0PheXaa: 0.0 ± 0.0
Gly
5.229GlyAla: 5.229 ± 1.104
0.101GlyCys: 0.101 ± 0.107
3.721GlyAsp: 3.721 ± 0.533
3.922GlyGlu: 3.922 ± 0.663
3.017GlyPhe: 3.017 ± 0.66
4.023GlyGly: 4.023 ± 0.95
1.106GlyHis: 1.106 ± 0.293
4.224GlyIle: 4.224 ± 0.883
5.531GlyLys: 5.531 ± 0.784
5.531GlyLeu: 5.531 ± 0.877
1.911GlyMet: 1.911 ± 0.403
3.821GlyAsn: 3.821 ± 0.875
0.905GlyPro: 0.905 ± 0.286
4.123GlyGln: 4.123 ± 0.568
2.816GlyArg: 2.816 ± 0.468
3.821GlySer: 3.821 ± 1.02
3.52GlyThr: 3.52 ± 0.6
4.224GlyVal: 4.224 ± 0.887
0.805GlyTrp: 0.805 ± 0.285
2.715GlyTyr: 2.715 ± 0.557
0.0GlyXaa: 0.0 ± 0.0
His
0.905HisAla: 0.905 ± 0.315
0.101HisCys: 0.101 ± 0.107
0.805HisAsp: 0.805 ± 0.313
1.006HisGlu: 1.006 ± 0.321
0.603HisPhe: 0.603 ± 0.287
1.207HisGly: 1.207 ± 0.274
0.402HisHis: 0.402 ± 0.213
0.704HisIle: 0.704 ± 0.233
0.805HisLys: 0.805 ± 0.281
1.207HisLeu: 1.207 ± 0.393
0.402HisMet: 0.402 ± 0.183
0.805HisAsn: 0.805 ± 0.273
1.207HisPro: 1.207 ± 0.342
0.503HisGln: 0.503 ± 0.178
0.603HisArg: 0.603 ± 0.257
0.905HisSer: 0.905 ± 0.288
0.603HisThr: 0.603 ± 0.244
0.905HisVal: 0.905 ± 0.34
0.101HisTrp: 0.101 ± 0.09
0.503HisTyr: 0.503 ± 0.228
0.0HisXaa: 0.0 ± 0.0
Ile
5.43IleAla: 5.43 ± 0.517
0.503IleCys: 0.503 ± 0.253
5.833IleAsp: 5.833 ± 0.739
5.632IleGlu: 5.632 ± 0.783
2.414IlePhe: 2.414 ± 0.535
4.626IleGly: 4.626 ± 0.568
0.503IleHis: 0.503 ± 0.229
4.224IleIle: 4.224 ± 0.869
6.134IleLys: 6.134 ± 0.694
3.821IleLeu: 3.821 ± 0.632
1.71IleMet: 1.71 ± 0.537
3.117IleAsn: 3.117 ± 0.968
1.911IlePro: 1.911 ± 0.573
3.218IleGln: 3.218 ± 0.608
2.414IleArg: 2.414 ± 0.442
4.023IleSer: 4.023 ± 0.892
4.123IleThr: 4.123 ± 0.757
3.821IleVal: 3.821 ± 0.652
0.805IleTrp: 0.805 ± 0.281
2.112IleTyr: 2.112 ± 0.513
0.0IleXaa: 0.0 ± 0.0
Lys
5.933LysAla: 5.933 ± 0.666
0.201LysCys: 0.201 ± 0.124
3.52LysAsp: 3.52 ± 0.56
4.827LysGlu: 4.827 ± 0.885
2.916LysPhe: 2.916 ± 0.593
4.626LysGly: 4.626 ± 0.814
1.307LysHis: 1.307 ± 0.44
5.129LysIle: 5.129 ± 0.753
7.542LysLys: 7.542 ± 1.286
5.028LysLeu: 5.028 ± 0.687
2.112LysMet: 2.112 ± 0.305
4.626LysAsn: 4.626 ± 0.827
2.816LysPro: 2.816 ± 0.522
4.626LysGln: 4.626 ± 0.725
4.425LysArg: 4.425 ± 0.669
4.525LysSer: 4.525 ± 0.676
5.229LysThr: 5.229 ± 0.946
5.129LysVal: 5.129 ± 0.966
1.106LysTrp: 1.106 ± 0.298
2.514LysTyr: 2.514 ± 0.552
0.0LysXaa: 0.0 ± 0.0
Leu
8.749LeuAla: 8.749 ± 0.883
0.704LeuCys: 0.704 ± 0.27
4.324LeuAsp: 4.324 ± 0.546
6.637LeuGlu: 6.637 ± 1.003
3.52LeuPhe: 3.52 ± 0.536
4.827LeuGly: 4.827 ± 0.987
1.106LeuHis: 1.106 ± 0.306
3.218LeuIle: 3.218 ± 0.465
7.542LeuLys: 7.542 ± 0.923
7.442LeuLeu: 7.442 ± 0.786
1.508LeuMet: 1.508 ± 0.429
4.425LeuAsn: 4.425 ± 0.619
2.916LeuPro: 2.916 ± 0.629
2.615LeuGln: 2.615 ± 0.538
4.023LeuArg: 4.023 ± 0.72
4.626LeuSer: 4.626 ± 0.976
5.43LeuThr: 5.43 ± 0.755
5.028LeuVal: 5.028 ± 0.658
0.704LeuTrp: 0.704 ± 0.247
1.207LeuTyr: 1.207 ± 0.436
0.0LeuXaa: 0.0 ± 0.0
Met
2.715MetAla: 2.715 ± 0.447
0.201MetCys: 0.201 ± 0.135
1.609MetAsp: 1.609 ± 0.348
1.408MetGlu: 1.408 ± 0.374
0.503MetPhe: 0.503 ± 0.176
1.508MetGly: 1.508 ± 0.663
0.201MetHis: 0.201 ± 0.127
1.911MetIle: 1.911 ± 0.568
2.212MetLys: 2.212 ± 0.551
1.81MetLeu: 1.81 ± 0.362
0.101MetMet: 0.101 ± 0.118
1.207MetAsn: 1.207 ± 0.468
0.905MetPro: 0.905 ± 0.264
0.603MetGln: 0.603 ± 0.199
0.905MetArg: 0.905 ± 0.311
1.911MetSer: 1.911 ± 0.384
3.218MetThr: 3.218 ± 0.432
1.609MetVal: 1.609 ± 0.422
0.402MetTrp: 0.402 ± 0.18
1.006MetTyr: 1.006 ± 0.418
0.0MetXaa: 0.0 ± 0.0
Asn
3.821AsnAla: 3.821 ± 0.809
0.603AsnCys: 0.603 ± 0.226
2.514AsnAsp: 2.514 ± 0.64
2.313AsnGlu: 2.313 ± 0.409
2.414AsnPhe: 2.414 ± 0.471
4.123AsnGly: 4.123 ± 0.651
0.603AsnHis: 0.603 ± 0.246
4.123AsnIle: 4.123 ± 0.874
3.922AsnLys: 3.922 ± 0.697
5.229AsnLeu: 5.229 ± 0.822
0.805AsnMet: 0.805 ± 0.329
2.615AsnAsn: 2.615 ± 0.534
2.615AsnPro: 2.615 ± 0.639
3.721AsnGln: 3.721 ± 0.618
2.816AsnArg: 2.816 ± 0.588
3.821AsnSer: 3.821 ± 0.678
2.916AsnThr: 2.916 ± 0.702
2.816AsnVal: 2.816 ± 0.49
0.805AsnTrp: 0.805 ± 0.236
2.514AsnTyr: 2.514 ± 0.573
0.0AsnXaa: 0.0 ± 0.0
Pro
1.71ProAla: 1.71 ± 0.407
0.201ProCys: 0.201 ± 0.118
2.313ProAsp: 2.313 ± 0.591
2.112ProGlu: 2.112 ± 0.606
1.106ProPhe: 1.106 ± 0.292
1.207ProGly: 1.207 ± 0.285
0.503ProHis: 0.503 ± 0.215
2.112ProIle: 2.112 ± 0.417
2.514ProLys: 2.514 ± 0.675
2.212ProLeu: 2.212 ± 0.396
1.207ProMet: 1.207 ± 0.298
1.911ProAsn: 1.911 ± 0.634
0.402ProPro: 0.402 ± 0.21
0.805ProGln: 0.805 ± 0.313
0.603ProArg: 0.603 ± 0.228
2.514ProSer: 2.514 ± 0.537
2.112ProThr: 2.112 ± 0.628
1.81ProVal: 1.81 ± 0.432
0.302ProTrp: 0.302 ± 0.192
0.603ProTyr: 0.603 ± 0.264
0.0ProXaa: 0.0 ± 0.0
Gln
4.224GlnAla: 4.224 ± 0.67
0.201GlnCys: 0.201 ± 0.138
2.615GlnAsp: 2.615 ± 0.447
2.615GlnGlu: 2.615 ± 0.613
1.207GlnPhe: 1.207 ± 0.408
1.307GlnGly: 1.307 ± 0.347
0.503GlnHis: 0.503 ± 0.324
3.017GlnIle: 3.017 ± 0.534
2.816GlnLys: 2.816 ± 0.507
4.123GlnLeu: 4.123 ± 0.655
0.905GlnMet: 0.905 ± 0.29
1.609GlnAsn: 1.609 ± 0.452
1.408GlnPro: 1.408 ± 0.342
1.911GlnGln: 1.911 ± 0.496
2.112GlnArg: 2.112 ± 0.473
3.62GlnSer: 3.62 ± 0.678
3.117GlnThr: 3.117 ± 0.81
2.816GlnVal: 2.816 ± 0.54
0.503GlnTrp: 0.503 ± 0.166
1.207GlnTyr: 1.207 ± 0.321
0.0GlnXaa: 0.0 ± 0.0
Arg
2.514ArgAla: 2.514 ± 0.529
0.402ArgCys: 0.402 ± 0.285
2.313ArgAsp: 2.313 ± 0.531
4.123ArgGlu: 4.123 ± 0.89
2.212ArgPhe: 2.212 ± 0.442
1.911ArgGly: 1.911 ± 0.371
1.207ArgHis: 1.207 ± 0.327
4.324ArgIle: 4.324 ± 0.706
3.218ArgLys: 3.218 ± 0.692
4.525ArgLeu: 4.525 ± 0.819
1.106ArgMet: 1.106 ± 0.336
2.916ArgAsn: 2.916 ± 0.634
1.307ArgPro: 1.307 ± 0.408
1.408ArgGln: 1.408 ± 0.452
2.112ArgArg: 2.112 ± 0.5
2.313ArgSer: 2.313 ± 0.413
3.117ArgThr: 3.117 ± 0.524
3.117ArgVal: 3.117 ± 0.503
0.402ArgTrp: 0.402 ± 0.175
2.313ArgTyr: 2.313 ± 0.469
0.0ArgXaa: 0.0 ± 0.0
Ser
5.732SerAla: 5.732 ± 1.448
0.402SerCys: 0.402 ± 0.174
3.62SerAsp: 3.62 ± 0.529
4.224SerGlu: 4.224 ± 0.674
3.017SerPhe: 3.017 ± 0.616
5.028SerGly: 5.028 ± 0.889
0.603SerHis: 0.603 ± 0.2
3.721SerIle: 3.721 ± 0.689
3.922SerLys: 3.922 ± 0.537
4.827SerLeu: 4.827 ± 0.921
2.313SerMet: 2.313 ± 0.501
3.419SerAsn: 3.419 ± 0.49
1.408SerPro: 1.408 ± 0.323
2.414SerGln: 2.414 ± 0.674
2.715SerArg: 2.715 ± 0.534
4.224SerSer: 4.224 ± 0.757
3.319SerThr: 3.319 ± 0.64
5.129SerVal: 5.129 ± 1.082
1.106SerTrp: 1.106 ± 0.335
2.816SerTyr: 2.816 ± 0.434
0.0SerXaa: 0.0 ± 0.0
Thr
5.833ThrAla: 5.833 ± 1.064
0.101ThrCys: 0.101 ± 0.116
3.721ThrAsp: 3.721 ± 0.616
3.821ThrGlu: 3.821 ± 0.767
2.212ThrPhe: 2.212 ± 0.501
5.028ThrGly: 5.028 ± 0.668
1.006ThrHis: 1.006 ± 0.355
3.419ThrIle: 3.419 ± 0.668
4.224ThrLys: 4.224 ± 0.623
4.726ThrLeu: 4.726 ± 0.815
0.905ThrMet: 0.905 ± 0.324
2.916ThrAsn: 2.916 ± 0.482
1.408ThrPro: 1.408 ± 0.314
2.112ThrGln: 2.112 ± 0.548
2.615ThrArg: 2.615 ± 0.506
4.023ThrSer: 4.023 ± 0.558
2.615ThrThr: 2.615 ± 0.532
6.838ThrVal: 6.838 ± 1.075
0.402ThrTrp: 0.402 ± 0.247
2.414ThrTyr: 2.414 ± 0.468
0.0ThrXaa: 0.0 ± 0.0
Val
5.833ValAla: 5.833 ± 0.682
0.302ValCys: 0.302 ± 0.164
5.632ValAsp: 5.632 ± 0.688
5.028ValGlu: 5.028 ± 0.834
2.715ValPhe: 2.715 ± 0.479
3.821ValGly: 3.821 ± 0.599
0.704ValHis: 0.704 ± 0.242
3.922ValIle: 3.922 ± 0.583
4.626ValLys: 4.626 ± 0.685
4.726ValLeu: 4.726 ± 0.68
1.408ValMet: 1.408 ± 0.452
3.62ValAsn: 3.62 ± 0.545
2.212ValPro: 2.212 ± 0.426
2.715ValGln: 2.715 ± 0.727
2.615ValArg: 2.615 ± 0.474
5.833ValSer: 5.833 ± 0.841
3.922ValThr: 3.922 ± 0.881
4.023ValVal: 4.023 ± 0.534
0.805ValTrp: 0.805 ± 0.262
2.313ValTyr: 2.313 ± 0.553
0.0ValXaa: 0.0 ± 0.0
Trp
1.106TrpAla: 1.106 ± 0.323
0.201TrpCys: 0.201 ± 0.135
0.603TrpAsp: 0.603 ± 0.215
1.207TrpGlu: 1.207 ± 0.437
0.603TrpPhe: 0.603 ± 0.271
1.106TrpGly: 1.106 ± 0.31
0.0TrpHis: 0.0 ± 0.0
0.805TrpIle: 0.805 ± 0.293
1.207TrpLys: 1.207 ± 0.297
0.503TrpLeu: 0.503 ± 0.214
0.201TrpMet: 0.201 ± 0.13
0.704TrpAsn: 0.704 ± 0.248
0.101TrpPro: 0.101 ± 0.09
0.503TrpGln: 0.503 ± 0.317
0.704TrpArg: 0.704 ± 0.249
0.704TrpSer: 0.704 ± 0.235
0.805TrpThr: 0.805 ± 0.224
0.503TrpVal: 0.503 ± 0.199
0.101TrpTrp: 0.101 ± 0.107
0.402TrpTyr: 0.402 ± 0.193
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.615TyrAla: 2.615 ± 0.472
0.101TyrCys: 0.101 ± 0.101
2.816TyrAsp: 2.816 ± 0.51
2.112TyrGlu: 2.112 ± 0.399
1.71TyrPhe: 1.71 ± 0.413
1.81TyrGly: 1.81 ± 0.438
0.503TyrHis: 0.503 ± 0.186
1.81TyrIle: 1.81 ± 0.487
2.212TyrLys: 2.212 ± 0.514
2.816TyrLeu: 2.816 ± 0.52
1.207TyrMet: 1.207 ± 0.361
1.207TyrAsn: 1.207 ± 0.338
1.408TyrPro: 1.408 ± 0.335
1.508TyrGln: 1.508 ± 0.371
2.414TyrArg: 2.414 ± 0.541
2.212TyrSer: 2.212 ± 0.473
2.112TyrThr: 2.112 ± 0.405
2.011TyrVal: 2.011 ± 0.323
0.503TyrTrp: 0.503 ± 0.215
0.905TyrTyr: 0.905 ± 0.222
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (9945 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski