Streptomyces phage Daubenski

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Samistivirus; unclassified Samistivirus

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 207 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2WHL3|A0A5Q2WHL3_9CAUD Uncharacterized protein OS=Streptomyces phage Daubenski OX=2653725 GN=63 PE=4 SV=1
MM1 pKa = 7.76SYY3 pKa = 10.54QLQVLADD10 pKa = 3.61NPFSYY15 pKa = 9.85WKK17 pKa = 10.48LDD19 pKa = 3.37EE20 pKa = 4.85TGPAFPDD27 pKa = 3.31SAGSLRR33 pKa = 11.84TADD36 pKa = 3.61LVGTITRR43 pKa = 11.84HH44 pKa = 5.3PALVTGSGSALLLNNTNQLQMDD66 pKa = 4.02DD67 pKa = 3.63PVFNKK72 pKa = 10.34GYY74 pKa = 8.92EE75 pKa = 3.89LRR77 pKa = 11.84QFSLEE82 pKa = 3.89AWVKK86 pKa = 8.64PVNVTGEE93 pKa = 4.22VALMSHH99 pKa = 6.44SSTYY103 pKa = 10.64DD104 pKa = 3.18GLVISPTNITFRR116 pKa = 11.84TKK118 pKa = 10.49YY119 pKa = 8.85LTAGTCEE126 pKa = 3.71VTYY129 pKa = 10.18EE130 pKa = 3.94YY131 pKa = 10.85DD132 pKa = 3.12ASKK135 pKa = 10.6SFHH138 pKa = 6.0VVGVHH143 pKa = 5.58TNAKK147 pKa = 9.52NSLYY151 pKa = 10.73VDD153 pKa = 4.01GVLVAEE159 pKa = 4.55TDD161 pKa = 3.61LTDD164 pKa = 3.53EE165 pKa = 4.29QQTDD169 pKa = 3.53VYY171 pKa = 11.61AFLTTDD177 pKa = 4.78LIAGQSSGVSTIALDD192 pKa = 3.65APAIYY197 pKa = 9.7TFALTRR203 pKa = 11.84DD204 pKa = 3.93AVAKK208 pKa = 10.25HH209 pKa = 5.74YY210 pKa = 11.12GFGIDD215 pKa = 3.23VDD217 pKa = 4.19SSEE220 pKa = 5.43GIAGYY225 pKa = 10.83NDD227 pKa = 4.38AIFWNFSDD235 pKa = 3.5EE236 pKa = 4.23TRR238 pKa = 11.84NIALKK243 pKa = 9.71QLWSSAADD251 pKa = 3.34WATGTLSDD259 pKa = 4.4TVIIDD264 pKa = 3.41DD265 pKa = 5.04TIAPGYY271 pKa = 8.47SQSEE275 pKa = 4.41SSVVEE280 pKa = 4.34DD281 pKa = 3.83GLIIPVYY288 pKa = 10.11TNTSLPGTWQASIAFNVIPEE308 pKa = 4.35TTISDD313 pKa = 4.26ARR315 pKa = 11.84INWKK319 pKa = 9.71GQGSYY324 pKa = 9.76TIEE327 pKa = 3.92YY328 pKa = 9.37SLDD331 pKa = 3.53GGTVWLTPSDD341 pKa = 3.83SGATTLDD348 pKa = 3.29NDD350 pKa = 3.64STIDD354 pKa = 3.59IRR356 pKa = 11.84ITFDD360 pKa = 2.97GGIVDD365 pKa = 3.79DD366 pKa = 4.41TSFVEE371 pKa = 4.57YY372 pKa = 10.33IEE374 pKa = 4.29VVAYY378 pKa = 10.01LDD380 pKa = 3.38KK381 pKa = 10.99YY382 pKa = 10.84FRR384 pKa = 11.84GSRR387 pKa = 11.84SDD389 pKa = 2.93RR390 pKa = 11.84TAQMSGTGVASDD402 pKa = 3.94NYY404 pKa = 10.54YY405 pKa = 10.98EE406 pKa = 4.7PIEE409 pKa = 4.06YY410 pKa = 10.75ADD412 pKa = 3.5INGLRR417 pKa = 11.84MISGAINVSLDD428 pKa = 3.04SSYY431 pKa = 11.6AGEE434 pKa = 4.52EE435 pKa = 4.18EE436 pKa = 4.57PGDD439 pKa = 3.61LFIRR443 pKa = 11.84GFDD446 pKa = 3.31MWVKK450 pKa = 8.58PTAGNILNATGVNVEE465 pKa = 4.15RR466 pKa = 11.84SGNTIVFSGFSAFTVNGASVSSGATVFGSDD496 pKa = 2.61SWYY499 pKa = 10.47HH500 pKa = 4.92IAGVFTIPANLALQIGSPGIIIAQLSAIYY529 pKa = 8.38DD530 pKa = 4.18TINLAGLQQMYY541 pKa = 10.23AAYY544 pKa = 10.26LGLPGLVVNDD554 pKa = 4.13SSDD557 pKa = 3.48VGIDD561 pKa = 3.2EE562 pKa = 4.72ATPTTNLYY570 pKa = 9.67AHH572 pKa = 6.59VWGITPAGG580 pKa = 3.48

Molecular weight: 62.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2WIB1|A0A5Q2WIB1_9CAUD Uncharacterized protein OS=Streptomyces phage Daubenski OX=2653725 GN=108 PE=4 SV=1
MM1 pKa = 7.74ALTGTNGQCWFQRR14 pKa = 11.84CSRR17 pKa = 11.84KK18 pKa = 8.66ATTTIRR24 pKa = 11.84YY25 pKa = 7.5YY26 pKa = 10.01NKK28 pKa = 10.43SVVVCNGHH36 pKa = 6.55KK37 pKa = 10.75NLDD40 pKa = 3.8GVGCPSSRR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84PKK52 pKa = 10.46SS53 pKa = 3.11

Molecular weight: 5.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

207

0

207

35038

33

2097

169.3

18.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.491 ± 0.352

1.053 ± 0.124

6.596 ± 0.187

6.756 ± 0.315

3.819 ± 0.124

7.435 ± 0.197

1.918 ± 0.155

5.271 ± 0.138

6.071 ± 0.314

7.301 ± 0.197

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.905 ± 0.126

4.532 ± 0.141

3.722 ± 0.161

3.228 ± 0.18

5.702 ± 0.198

6.016 ± 0.277

6.316 ± 0.349

7.226 ± 0.216

1.889 ± 0.097

3.753 ± 0.176
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski