Amino acid dipepetide frequency for Streptomyces phage Daubenski

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.249AlaAla: 7.249 ± 0.801
0.828AlaCys: 0.828 ± 0.192
5.252AlaAsp: 5.252 ± 0.424
5.623AlaGlu: 5.623 ± 0.496
3.311AlaPhe: 3.311 ± 0.31
6.593AlaGly: 6.593 ± 0.572
1.256AlaHis: 1.256 ± 0.167
4.938AlaIle: 4.938 ± 0.524
4.938AlaLys: 4.938 ± 0.387
6.993AlaLeu: 6.993 ± 0.583
2.455AlaMet: 2.455 ± 0.278
3.482AlaAsn: 3.482 ± 0.4
2.911AlaPro: 2.911 ± 0.388
3.054AlaGln: 3.054 ± 0.399
4.567AlaArg: 4.567 ± 0.398
4.624AlaSer: 4.624 ± 0.42
5.708AlaThr: 5.708 ± 0.709
6.136AlaVal: 6.136 ± 0.487
1.798AlaTrp: 1.798 ± 0.189
3.168AlaTyr: 3.168 ± 0.3
0.0AlaXaa: 0.0 ± 0.0
Cys
0.599CysAla: 0.599 ± 0.137
0.2CysCys: 0.2 ± 0.09
0.628CysAsp: 0.628 ± 0.137
0.514CysGlu: 0.514 ± 0.139
0.257CysPhe: 0.257 ± 0.098
1.227CysGly: 1.227 ± 0.26
0.285CysHis: 0.285 ± 0.092
0.428CysIle: 0.428 ± 0.12
0.97CysLys: 0.97 ± 0.207
0.771CysLeu: 0.771 ± 0.152
0.285CysMet: 0.285 ± 0.092
0.628CysAsn: 0.628 ± 0.161
0.542CysPro: 0.542 ± 0.154
0.257CysGln: 0.257 ± 0.1
0.685CysArg: 0.685 ± 0.151
0.571CysSer: 0.571 ± 0.135
0.514CysThr: 0.514 ± 0.114
0.628CysVal: 0.628 ± 0.153
0.2CysTrp: 0.2 ± 0.082
0.342CysTyr: 0.342 ± 0.088
0.0CysXaa: 0.0 ± 0.0
Asp
6.051AspAla: 6.051 ± 0.542
0.599AspCys: 0.599 ± 0.124
4.253AspAsp: 4.253 ± 0.404
5.451AspGlu: 5.451 ± 0.482
3.082AspPhe: 3.082 ± 0.324
5.166AspGly: 5.166 ± 0.469
1.056AspHis: 1.056 ± 0.16
3.739AspIle: 3.739 ± 0.369
3.767AspLys: 3.767 ± 0.366
4.395AspLeu: 4.395 ± 0.399
2.141AspMet: 2.141 ± 0.267
2.711AspAsn: 2.711 ± 0.244
2.312AspPro: 2.312 ± 0.277
1.484AspGln: 1.484 ± 0.188
3.14AspArg: 3.14 ± 0.33
3.91AspSer: 3.91 ± 0.373
3.853AspThr: 3.853 ± 0.386
4.224AspVal: 4.224 ± 0.417
1.827AspTrp: 1.827 ± 0.249
2.797AspTyr: 2.797 ± 0.301
0.0AspXaa: 0.0 ± 0.0
Glu
6.479GluAla: 6.479 ± 0.537
0.571GluCys: 0.571 ± 0.134
4.452GluAsp: 4.452 ± 0.39
4.538GluGlu: 4.538 ± 0.418
2.512GluPhe: 2.512 ± 0.269
4.681GluGly: 4.681 ± 0.465
1.627GluHis: 1.627 ± 0.275
4.31GluIle: 4.31 ± 0.292
4.053GluLys: 4.053 ± 0.371
4.966GluLeu: 4.966 ± 0.4
2.255GluMet: 2.255 ± 0.275
3.082GluAsn: 3.082 ± 0.339
1.941GluPro: 1.941 ± 0.344
2.626GluGln: 2.626 ± 0.295
4.024GluArg: 4.024 ± 0.403
3.082GluSer: 3.082 ± 0.298
3.511GluThr: 3.511 ± 0.341
4.795GluVal: 4.795 ± 0.463
1.456GluTrp: 1.456 ± 0.248
2.597GluTyr: 2.597 ± 0.408
0.0GluXaa: 0.0 ± 0.0
Phe
2.769PheAla: 2.769 ± 0.27
0.4PheCys: 0.4 ± 0.137
3.368PheAsp: 3.368 ± 0.302
3.368PheGlu: 3.368 ± 0.327
1.627PhePhe: 1.627 ± 0.222
2.626PheGly: 2.626 ± 0.242
0.742PheHis: 0.742 ± 0.165
2.026PheIle: 2.026 ± 0.285
1.998PheLys: 1.998 ± 0.25
2.512PheLeu: 2.512 ± 0.314
0.942PheMet: 0.942 ± 0.174
1.741PheAsn: 1.741 ± 0.19
1.113PhePro: 1.113 ± 0.171
1.142PheGln: 1.142 ± 0.18
2.112PheArg: 2.112 ± 0.263
2.797PheSer: 2.797 ± 0.337
2.683PheThr: 2.683 ± 0.293
2.569PheVal: 2.569 ± 0.269
0.457PheTrp: 0.457 ± 0.14
1.199PheTyr: 1.199 ± 0.167
0.0PheXaa: 0.0 ± 0.0
Gly
5.309GlyAla: 5.309 ± 0.519
0.714GlyCys: 0.714 ± 0.129
4.338GlyAsp: 4.338 ± 0.394
4.196GlyGlu: 4.196 ± 0.332
3.14GlyPhe: 3.14 ± 0.275
4.966GlyGly: 4.966 ± 0.451
1.912GlyHis: 1.912 ± 0.273
4.338GlyIle: 4.338 ± 0.355
5.109GlyLys: 5.109 ± 0.495
5.423GlyLeu: 5.423 ± 0.487
2.54GlyMet: 2.54 ± 0.275
3.453GlyAsn: 3.453 ± 0.336
2.055GlyPro: 2.055 ± 0.27
2.512GlyGln: 2.512 ± 0.249
4.167GlyArg: 4.167 ± 0.372
4.481GlySer: 4.481 ± 0.564
5.566GlyThr: 5.566 ± 0.754
5.879GlyVal: 5.879 ± 0.403
1.341GlyTrp: 1.341 ± 0.203
2.911GlyTyr: 2.911 ± 0.273
0.0GlyXaa: 0.0 ± 0.0
His
1.284HisAla: 1.284 ± 0.189
0.285HisCys: 0.285 ± 0.103
1.399HisAsp: 1.399 ± 0.217
1.085HisGlu: 1.085 ± 0.197
0.599HisPhe: 0.599 ± 0.134
1.37HisGly: 1.37 ± 0.196
0.571HisHis: 0.571 ± 0.133
1.113HisIle: 1.113 ± 0.155
1.056HisLys: 1.056 ± 0.157
1.598HisLeu: 1.598 ± 0.206
0.628HisMet: 0.628 ± 0.141
0.656HisAsn: 0.656 ± 0.149
0.799HisPro: 0.799 ± 0.165
0.571HisGln: 0.571 ± 0.123
1.712HisArg: 1.712 ± 0.284
1.227HisSer: 1.227 ± 0.198
0.771HisThr: 0.771 ± 0.194
1.256HisVal: 1.256 ± 0.196
0.228HisTrp: 0.228 ± 0.088
0.97HisTyr: 0.97 ± 0.196
0.0HisXaa: 0.0 ± 0.0
Ile
4.624IleAla: 4.624 ± 0.373
0.628IleCys: 0.628 ± 0.141
4.138IleAsp: 4.138 ± 0.298
3.767IleGlu: 3.767 ± 0.352
1.598IlePhe: 1.598 ± 0.218
3.682IleGly: 3.682 ± 0.3
1.17IleHis: 1.17 ± 0.208
2.426IleIle: 2.426 ± 0.285
3.339IleLys: 3.339 ± 0.316
3.625IleLeu: 3.625 ± 0.363
1.256IleMet: 1.256 ± 0.193
2.084IleAsn: 2.084 ± 0.303
2.512IlePro: 2.512 ± 0.277
1.741IleGln: 1.741 ± 0.238
3.111IleArg: 3.111 ± 0.284
3.054IleSer: 3.054 ± 0.232
3.168IleThr: 3.168 ± 0.337
4.338IleVal: 4.338 ± 0.357
0.771IleTrp: 0.771 ± 0.147
1.684IleTyr: 1.684 ± 0.282
0.0IleXaa: 0.0 ± 0.0
Lys
5.594LysAla: 5.594 ± 0.5
0.828LysCys: 0.828 ± 0.21
3.653LysAsp: 3.653 ± 0.465
3.996LysGlu: 3.996 ± 0.42
1.884LysPhe: 1.884 ± 0.288
3.568LysGly: 3.568 ± 0.32
1.056LysHis: 1.056 ± 0.169
3.025LysIle: 3.025 ± 0.295
4.053LysLys: 4.053 ± 0.487
4.167LysLeu: 4.167 ± 0.373
2.169LysMet: 2.169 ± 0.252
3.254LysAsn: 3.254 ± 0.325
2.141LysPro: 2.141 ± 0.306
2.226LysGln: 2.226 ± 0.287
3.625LysArg: 3.625 ± 0.356
3.311LysSer: 3.311 ± 0.293
3.682LysThr: 3.682 ± 0.311
4.595LysVal: 4.595 ± 0.379
1.17LysTrp: 1.17 ± 0.205
2.711LysTyr: 2.711 ± 0.301
0.0LysXaa: 0.0 ± 0.0
Leu
7.278LeuAla: 7.278 ± 0.479
0.97LeuCys: 0.97 ± 0.188
4.966LeuAsp: 4.966 ± 0.429
5.223LeuGlu: 5.223 ± 0.457
2.512LeuPhe: 2.512 ± 0.265
4.938LeuGly: 4.938 ± 0.433
1.313LeuHis: 1.313 ± 0.209
3.939LeuIle: 3.939 ± 0.332
4.224LeuLys: 4.224 ± 0.354
4.538LeuLeu: 4.538 ± 0.428
2.026LeuMet: 2.026 ± 0.287
3.767LeuAsn: 3.767 ± 0.329
3.082LeuPro: 3.082 ± 0.271
1.998LeuGln: 1.998 ± 0.288
4.081LeuArg: 4.081 ± 0.345
4.852LeuSer: 4.852 ± 0.295
4.652LeuThr: 4.652 ± 0.322
5.052LeuVal: 5.052 ± 0.462
1.37LeuTrp: 1.37 ± 0.198
2.226LeuTyr: 2.226 ± 0.226
0.0LeuXaa: 0.0 ± 0.0
Met
3.396MetAla: 3.396 ± 0.302
0.371MetCys: 0.371 ± 0.115
1.456MetAsp: 1.456 ± 0.24
1.541MetGlu: 1.541 ± 0.228
0.571MetPhe: 0.571 ± 0.144
2.426MetGly: 2.426 ± 0.37
0.542MetHis: 0.542 ± 0.143
1.341MetIle: 1.341 ± 0.169
1.684MetLys: 1.684 ± 0.227
1.912MetLeu: 1.912 ± 0.232
0.799MetMet: 0.799 ± 0.168
1.37MetAsn: 1.37 ± 0.197
1.17MetPro: 1.17 ± 0.206
0.799MetGln: 0.799 ± 0.161
2.226MetArg: 2.226 ± 0.249
2.283MetSer: 2.283 ± 0.329
2.226MetThr: 2.226 ± 0.226
1.541MetVal: 1.541 ± 0.209
0.285MetTrp: 0.285 ± 0.087
1.113MetTyr: 1.113 ± 0.178
0.0MetXaa: 0.0 ± 0.0
Asn
4.081AsnAla: 4.081 ± 0.443
0.342AsnCys: 0.342 ± 0.084
3.025AsnAsp: 3.025 ± 0.303
2.826AsnGlu: 2.826 ± 0.347
1.541AsnPhe: 1.541 ± 0.222
3.767AsnGly: 3.767 ± 0.344
1.056AsnHis: 1.056 ± 0.163
2.397AsnIle: 2.397 ± 0.221
2.968AsnLys: 2.968 ± 0.394
3.453AsnLeu: 3.453 ± 0.351
1.027AsnMet: 1.027 ± 0.175
2.084AsnAsn: 2.084 ± 0.299
1.969AsnPro: 1.969 ± 0.248
1.313AsnGln: 1.313 ± 0.226
2.283AsnArg: 2.283 ± 0.226
2.255AsnSer: 2.255 ± 0.282
2.883AsnThr: 2.883 ± 0.344
3.254AsnVal: 3.254 ± 0.354
0.942AsnTrp: 0.942 ± 0.155
1.855AsnTyr: 1.855 ± 0.245
0.0AsnXaa: 0.0 ± 0.0
Pro
2.683ProAla: 2.683 ± 0.262
0.2ProCys: 0.2 ± 0.078
2.911ProAsp: 2.911 ± 0.335
2.369ProGlu: 2.369 ± 0.292
1.627ProPhe: 1.627 ± 0.201
3.339ProGly: 3.339 ± 0.341
0.599ProHis: 0.599 ± 0.12
1.77ProIle: 1.77 ± 0.204
2.141ProLys: 2.141 ± 0.314
2.312ProLeu: 2.312 ± 0.244
0.799ProMet: 0.799 ± 0.14
1.798ProAsn: 1.798 ± 0.246
1.284ProPro: 1.284 ± 0.243
0.771ProGln: 0.771 ± 0.17
2.255ProArg: 2.255 ± 0.291
2.255ProSer: 2.255 ± 0.415
2.711ProThr: 2.711 ± 0.392
3.682ProVal: 3.682 ± 0.336
0.4ProTrp: 0.4 ± 0.1
1.313ProTyr: 1.313 ± 0.217
0.0ProXaa: 0.0 ± 0.0
Gln
3.368GlnAla: 3.368 ± 0.422
0.428GlnCys: 0.428 ± 0.126
1.284GlnAsp: 1.284 ± 0.16
2.397GlnGlu: 2.397 ± 0.254
1.57GlnPhe: 1.57 ± 0.207
2.055GlnGly: 2.055 ± 0.245
0.542GlnHis: 0.542 ± 0.146
1.627GlnIle: 1.627 ± 0.216
2.026GlnLys: 2.026 ± 0.349
2.94GlnLeu: 2.94 ± 0.298
1.056GlnMet: 1.056 ± 0.194
1.256GlnAsn: 1.256 ± 0.211
0.942GlnPro: 0.942 ± 0.166
1.056GlnGln: 1.056 ± 0.293
2.112GlnArg: 2.112 ± 0.333
1.884GlnSer: 1.884 ± 0.186
1.655GlnThr: 1.655 ± 0.265
2.397GlnVal: 2.397 ± 0.247
0.485GlnTrp: 0.485 ± 0.115
1.199GlnTyr: 1.199 ± 0.17
0.0GlnXaa: 0.0 ± 0.0
Arg
4.395ArgAla: 4.395 ± 0.432
0.457ArgCys: 0.457 ± 0.153
3.254ArgAsp: 3.254 ± 0.379
3.853ArgGlu: 3.853 ± 0.339
2.397ArgPhe: 2.397 ± 0.32
4.31ArgGly: 4.31 ± 0.326
0.771ArgHis: 0.771 ± 0.152
2.997ArgIle: 2.997 ± 0.326
3.625ArgLys: 3.625 ± 0.372
4.281ArgLeu: 4.281 ± 0.355
2.312ArgMet: 2.312 ± 0.262
2.797ArgAsn: 2.797 ± 0.287
2.226ArgPro: 2.226 ± 0.297
2.169ArgGln: 2.169 ± 0.313
3.596ArgArg: 3.596 ± 0.474
3.311ArgSer: 3.311 ± 0.255
3.111ArgThr: 3.111 ± 0.322
3.568ArgVal: 3.568 ± 0.274
0.97ArgTrp: 0.97 ± 0.179
2.626ArgTyr: 2.626 ± 0.322
0.0ArgXaa: 0.0 ± 0.0
Ser
4.738SerAla: 4.738 ± 0.579
0.628SerCys: 0.628 ± 0.141
4.424SerAsp: 4.424 ± 0.378
3.225SerGlu: 3.225 ± 0.269
2.397SerPhe: 2.397 ± 0.234
5.508SerGly: 5.508 ± 0.691
1.284SerHis: 1.284 ± 0.208
2.597SerIle: 2.597 ± 0.285
3.682SerLys: 3.682 ± 0.344
4.909SerLeu: 4.909 ± 0.448
1.855SerMet: 1.855 ± 0.201
2.455SerAsn: 2.455 ± 0.349
2.283SerPro: 2.283 ± 0.285
1.827SerGln: 1.827 ± 0.205
2.997SerArg: 2.997 ± 0.276
4.338SerSer: 4.338 ± 0.575
3.796SerThr: 3.796 ± 0.566
4.367SerVal: 4.367 ± 0.424
0.999SerTrp: 0.999 ± 0.161
1.855SerTyr: 1.855 ± 0.239
0.0SerXaa: 0.0 ± 0.0
Thr
5.08ThrAla: 5.08 ± 0.779
0.685ThrCys: 0.685 ± 0.134
4.138ThrAsp: 4.138 ± 0.359
3.568ThrGlu: 3.568 ± 0.336
2.455ThrPhe: 2.455 ± 0.234
5.109ThrGly: 5.109 ± 0.619
0.856ThrHis: 0.856 ± 0.166
3.91ThrIle: 3.91 ± 0.386
3.425ThrLys: 3.425 ± 0.283
4.652ThrLeu: 4.652 ± 0.335
1.37ThrMet: 1.37 ± 0.211
3.082ThrAsn: 3.082 ± 0.583
3.197ThrPro: 3.197 ± 0.397
2.198ThrGln: 2.198 ± 0.313
2.883ThrArg: 2.883 ± 0.308
3.482ThrSer: 3.482 ± 0.372
4.338ThrThr: 4.338 ± 0.698
5.166ThrVal: 5.166 ± 0.5
1.456ThrTrp: 1.456 ± 0.182
2.112ThrTyr: 2.112 ± 0.282
0.0ThrXaa: 0.0 ± 0.0
Val
5.594ValAla: 5.594 ± 0.394
0.828ValCys: 0.828 ± 0.162
5.109ValAsp: 5.109 ± 0.531
5.623ValGlu: 5.623 ± 0.433
2.854ValPhe: 2.854 ± 0.286
4.31ValGly: 4.31 ± 0.334
1.484ValHis: 1.484 ± 0.214
3.539ValIle: 3.539 ± 0.324
4.624ValLys: 4.624 ± 0.381
4.995ValLeu: 4.995 ± 0.443
1.627ValMet: 1.627 ± 0.207
2.968ValAsn: 2.968 ± 0.319
2.711ValPro: 2.711 ± 0.261
2.455ValGln: 2.455 ± 0.252
4.452ValArg: 4.452 ± 0.34
4.938ValSer: 4.938 ± 0.445
4.024ValThr: 4.024 ± 0.573
5.537ValVal: 5.537 ± 0.499
1.627ValTrp: 1.627 ± 0.22
2.968ValTyr: 2.968 ± 0.33
0.0ValXaa: 0.0 ± 0.0
Trp
1.57TrpAla: 1.57 ± 0.21
0.228TrpCys: 0.228 ± 0.08
1.113TrpAsp: 1.113 ± 0.178
1.684TrpGlu: 1.684 ± 0.241
0.771TrpPhe: 0.771 ± 0.14
1.541TrpGly: 1.541 ± 0.266
0.428TrpHis: 0.428 ± 0.12
0.656TrpIle: 0.656 ± 0.128
0.97TrpLys: 0.97 ± 0.178
1.77TrpLeu: 1.77 ± 0.256
0.571TrpMet: 0.571 ± 0.155
0.97TrpAsn: 0.97 ± 0.164
0.457TrpPro: 0.457 ± 0.132
0.685TrpGln: 0.685 ± 0.156
0.913TrpArg: 0.913 ± 0.149
0.913TrpSer: 0.913 ± 0.165
1.456TrpThr: 1.456 ± 0.22
0.942TrpVal: 0.942 ± 0.158
0.485TrpTrp: 0.485 ± 0.104
0.771TrpTyr: 0.771 ± 0.138
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.769TyrAla: 2.769 ± 0.302
0.342TyrCys: 0.342 ± 0.096
2.797TyrAsp: 2.797 ± 0.365
2.711TyrGlu: 2.711 ± 0.316
1.484TyrPhe: 1.484 ± 0.247
3.168TyrGly: 3.168 ± 0.328
0.628TyrHis: 0.628 ± 0.12
1.598TyrIle: 1.598 ± 0.224
2.055TyrLys: 2.055 ± 0.276
2.797TyrLeu: 2.797 ± 0.297
0.913TyrMet: 0.913 ± 0.159
1.684TyrAsn: 1.684 ± 0.232
1.57TyrPro: 1.57 ± 0.279
1.399TyrGln: 1.399 ± 0.191
2.084TyrArg: 2.084 ± 0.259
2.597TyrSer: 2.597 ± 0.273
2.854TyrThr: 2.854 ± 0.284
2.34TyrVal: 2.34 ± 0.331
0.628TyrTrp: 0.628 ± 0.127
1.113TyrTyr: 1.113 ± 0.212
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 207 proteins (35038 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski