Cladophialophora psammophila CBS 110553
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13417 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W9X6U1|W9X6U1_9EURO L-lactate dehydrogenase OS=Cladophialophora psammophila CBS 110553 OX=1182543 GN=A1O5_04199 PE=3 SV=1
MM1 pKa = 7.71 RR2 pKa = 11.84 CDD4 pKa = 4.79 GNDD7 pKa = 3.31 PCAKK11 pKa = 10.11 CLSTDD16 pKa = 3.54 SACQYY21 pKa = 9.65 SAPASSSSVPSDD33 pKa = 3.2 TLSARR38 pKa = 11.84 TPTEE42 pKa = 3.92 AYY44 pKa = 10.24 SFQATGDD51 pKa = 3.64 FNAPLLVPTTANVAGEE67 pKa = 4.28 FASQYY72 pKa = 10.37 PGLHH76 pKa = 6.47 LEE78 pKa = 4.55 ASSTGPSNCEE88 pKa = 3.75 TVDD91 pKa = 5.55 LDD93 pKa = 5.38 SLDD96 pKa = 3.77 NDD98 pKa = 3.76 DD99 pKa = 5.29 RR100 pKa = 11.84 NVPRR104 pKa = 11.84 LGLSRR109 pKa = 11.84 PAASTLDD116 pKa = 3.69 SMSCDD121 pKa = 3.39 VLAMPISPDD130 pKa = 3.57 DD131 pKa = 4.39 DD132 pKa = 3.52 FLQFFSAEE140 pKa = 3.93 GMRR143 pKa = 11.84 GQWQTTPVVV152 pKa = 3.29
Molecular weight: 16.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.708
IPC2_protein 3.834
IPC_protein 3.821
Toseland 3.592
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.808
Rodwell 3.643
Grimsley 3.503
Solomon 3.821
Lehninger 3.77
Nozaki 3.948
DTASelect 4.228
Thurlkill 3.668
EMBOSS 3.808
Sillero 3.948
Patrickios 0.947
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.833
Protein with the highest isoelectric point:
>tr|W9X2G0|W9X2G0_9EURO S-formylglutathione hydrolase OS=Cladophialophora psammophila CBS 110553 OX=1182543 GN=A1O5_02634 PE=3 SV=1
MM1 pKa = 6.54 NTARR5 pKa = 11.84 LLRR8 pKa = 11.84 LQPLRR13 pKa = 11.84 AVGLRR18 pKa = 11.84 QAAFRR23 pKa = 11.84 QSAQKK28 pKa = 8.28 QTARR32 pKa = 11.84 PFHH35 pKa = 5.15 NTRR38 pKa = 11.84 TRR40 pKa = 11.84 FRR42 pKa = 11.84 TKK44 pKa = 9.05 VSKK47 pKa = 10.65 RR48 pKa = 11.84 ISS50 pKa = 3.18
Molecular weight: 5.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.954
ProMoST 13.451
Dawson 12.954
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.574
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.451
Sillero 12.954
Patrickios 12.31
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.172
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13417
0
13417
6277743
49
6117
467.9
51.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.628 ± 0.016
1.269 ± 0.008
5.607 ± 0.014
6.105 ± 0.021
3.818 ± 0.014
6.897 ± 0.019
2.443 ± 0.01
5.035 ± 0.013
4.748 ± 0.016
9.09 ± 0.022
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.2 ± 0.007
3.61 ± 0.01
5.94 ± 0.019
4.073 ± 0.014
6.124 ± 0.016
7.981 ± 0.023
5.904 ± 0.015
6.202 ± 0.015
1.519 ± 0.008
2.806 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here