Porcine stool-associated circular virus 4

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 7.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W8TKM5|W8TKM5_9CIRC Replication-associated protein OS=Porcine stool-associated circular virus 4 OX=1475062 PE=4 SV=1
MM1 pKa = 7.86PARR4 pKa = 11.84NPEE7 pKa = 3.69GRR9 pKa = 11.84PQRR12 pKa = 11.84FRR14 pKa = 11.84FACITAWNMDD24 pKa = 4.04AFDD27 pKa = 5.42LDD29 pKa = 3.74WPQRR33 pKa = 11.84MHH35 pKa = 7.46DD36 pKa = 3.95LGLSYY41 pKa = 10.97VVIGRR46 pKa = 11.84EE47 pKa = 3.86TCPRR51 pKa = 11.84TGRR54 pKa = 11.84RR55 pKa = 11.84HH56 pKa = 4.92LQCYY60 pKa = 9.5FEE62 pKa = 4.37ATNPKK67 pKa = 8.86TVTAWQDD74 pKa = 3.18VVMDD78 pKa = 4.11KK79 pKa = 10.78PRR81 pKa = 11.84AHH83 pKa = 6.16VEE85 pKa = 3.9CRR87 pKa = 11.84AAWKK91 pKa = 10.15PEE93 pKa = 3.79GEE95 pKa = 5.2DD96 pKa = 3.11IAEE99 pKa = 4.19NQGDD103 pKa = 4.02KK104 pKa = 11.14AADD107 pKa = 3.59YY108 pKa = 10.18CKK110 pKa = 10.65KK111 pKa = 10.3EE112 pKa = 3.8GDD114 pKa = 3.72FIEE117 pKa = 4.82YY118 pKa = 7.81GTLAPGQGARR128 pKa = 11.84MDD130 pKa = 4.16LADD133 pKa = 4.95CKK135 pKa = 10.88TMIDD139 pKa = 4.09EE140 pKa = 4.9GKK142 pKa = 10.74QMIDD146 pKa = 3.16LYY148 pKa = 9.97EE149 pKa = 4.1KK150 pKa = 10.93HH151 pKa = 6.54FGTCVRR157 pKa = 11.84CHH159 pKa = 6.86RR160 pKa = 11.84GLMLYY165 pKa = 10.28KK166 pKa = 10.64DD167 pKa = 3.46LVDD170 pKa = 3.82RR171 pKa = 11.84KK172 pKa = 9.57RR173 pKa = 11.84RR174 pKa = 11.84KK175 pKa = 8.6EE176 pKa = 3.8AEE178 pKa = 4.2PEE180 pKa = 3.74PKK182 pKa = 10.24EE183 pKa = 3.51IVVYY187 pKa = 10.48VGASGSGKK195 pKa = 8.84SHH197 pKa = 6.67HH198 pKa = 6.92CWHH201 pKa = 7.23DD202 pKa = 3.02PDD204 pKa = 4.03YY205 pKa = 11.15RR206 pKa = 11.84RR207 pKa = 11.84SGYY210 pKa = 10.28KK211 pKa = 10.07YY212 pKa = 10.21PLLAEE217 pKa = 4.11NKK219 pKa = 8.11VWFDD223 pKa = 3.42GYY225 pKa = 10.87EE226 pKa = 4.11GEE228 pKa = 4.22EE229 pKa = 4.12VLWVDD234 pKa = 3.79EE235 pKa = 4.38FRR237 pKa = 11.84GSVFPFGLFLQVTDD251 pKa = 2.78KK252 pKa = 10.38WGARR256 pKa = 11.84VEE258 pKa = 4.36VKK260 pKa = 10.49GGSIEE265 pKa = 4.25TFFKK269 pKa = 10.73KK270 pKa = 10.62VLISTTVPPGEE281 pKa = 4.13WYY283 pKa = 10.48KK284 pKa = 11.27NCSNYY289 pKa = 10.47LKK291 pKa = 10.52NPKK294 pKa = 9.09QLWRR298 pKa = 11.84RR299 pKa = 11.84LTKK302 pKa = 10.28VYY304 pKa = 10.1WLGPVEE310 pKa = 4.03QDD312 pKa = 3.51DD313 pKa = 4.81DD314 pKa = 4.4GFTIYY319 pKa = 10.24PEE321 pKa = 4.53PEE323 pKa = 5.18LIPDD327 pKa = 4.21PEE329 pKa = 5.89HH330 pKa = 7.0DD331 pKa = 3.29TRR333 pKa = 11.84TNPFYY338 pKa = 11.11DD339 pKa = 3.3GVGTHH344 pKa = 5.99YY345 pKa = 10.45PRR347 pKa = 11.84EE348 pKa = 4.12

Molecular weight: 40.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W8TKM5|W8TKM5_9CIRC Replication-associated protein OS=Porcine stool-associated circular virus 4 OX=1475062 PE=4 SV=1
MM1 pKa = 7.26HH2 pKa = 7.81RR3 pKa = 11.84SGDD6 pKa = 3.64LALFLYY12 pKa = 9.97VVVRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84YY19 pKa = 8.05RR20 pKa = 11.84KK21 pKa = 9.39SRR23 pKa = 11.84GRR25 pKa = 11.84LLRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84ARR32 pKa = 11.84RR33 pKa = 11.84YY34 pKa = 9.62RR35 pKa = 11.84LSKK38 pKa = 9.65SMRR41 pKa = 11.84MRR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84TIRR50 pKa = 11.84RR51 pKa = 11.84NRR53 pKa = 11.84RR54 pKa = 11.84RR55 pKa = 11.84GGLTVSGSAEE65 pKa = 3.94YY66 pKa = 9.46PLSYY70 pKa = 10.96GSLLPVEE77 pKa = 5.45RR78 pKa = 11.84KK79 pKa = 10.1AKK81 pKa = 10.21GSDD84 pKa = 2.87VGVPHH89 pKa = 7.62DD90 pKa = 5.38GFCTWSICPLPMGVPQVIGDD110 pKa = 3.97LSVVSFWADD119 pKa = 3.06LNTSYY124 pKa = 11.7GEE126 pKa = 4.08RR127 pKa = 11.84YY128 pKa = 8.92KK129 pKa = 10.95PKK131 pKa = 10.24IGLGQNASDD140 pKa = 3.96EE141 pKa = 4.57TKK143 pKa = 9.88MLCDD147 pKa = 4.51LPVCLSSFTVRR158 pKa = 11.84SIVKK162 pKa = 10.5CEE164 pKa = 4.1TLWKK168 pKa = 10.14LYY170 pKa = 10.57KK171 pKa = 10.25LYY173 pKa = 10.4RR174 pKa = 11.84ISWVSVTFTVPEE186 pKa = 4.0FTGGQRR192 pKa = 11.84NHH194 pKa = 6.08NLYY197 pKa = 10.87LEE199 pKa = 4.41WTHH202 pKa = 6.87LPKK205 pKa = 10.48ARR207 pKa = 11.84CAAYY211 pKa = 9.25EE212 pKa = 4.0DD213 pKa = 4.83CIGMVVSGNGKK224 pKa = 10.16SSDD227 pKa = 3.33TGGWNWIYY235 pKa = 10.88NPPDD239 pKa = 3.13VATACSIDD247 pKa = 3.16GRR249 pKa = 11.84KK250 pKa = 9.61NGRR253 pKa = 11.84NGWRR257 pKa = 11.84RR258 pKa = 11.84AQLAYY263 pKa = 10.23NHH265 pKa = 7.09PVTISWRR272 pKa = 11.84PKK274 pKa = 9.17HH275 pKa = 6.65AEE277 pKa = 4.12LIQDD281 pKa = 3.74HH282 pKa = 5.97VNYY285 pKa = 9.81WDD287 pKa = 3.55ATSASTGGTKK297 pKa = 10.51VMDD300 pKa = 3.93LWPTKK305 pKa = 10.74DD306 pKa = 3.16KK307 pKa = 10.74FVRR310 pKa = 11.84SYY312 pKa = 11.34LPTDD316 pKa = 3.67IDD318 pKa = 3.53QQMTAEE324 pKa = 4.08RR325 pKa = 11.84QVWCGPVIRR334 pKa = 11.84LIDD337 pKa = 3.9ADD339 pKa = 3.52ITAAAIPSQPPTNTWYY355 pKa = 9.25SQYY358 pKa = 10.98GIRR361 pKa = 11.84CTCVVKK367 pKa = 10.83LKK369 pKa = 10.17FRR371 pKa = 11.84NMDD374 pKa = 3.45AADD377 pKa = 4.77PIFPEE382 pKa = 4.39YY383 pKa = 11.0VPP385 pKa = 4.56

Molecular weight: 43.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

733

348

385

366.5

42.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.73 ± 0.168

2.865 ± 0.005

6.548 ± 0.754

5.321 ± 1.697

3.274 ± 0.645

7.776 ± 0.347

2.319 ± 0.345

3.956 ± 0.495

5.866 ± 0.642

6.821 ± 0.49

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.319 ± 0.013

3.138 ± 0.344

6.412 ± 0.302

2.729 ± 0.09

8.868 ± 1.049

5.184 ± 1.618

5.73 ± 0.347

7.094 ± 0.302

3.274 ± 0.071

4.775 ± 0.069
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski