Wenling tombus-like virus 5

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGT2|A0A1L3KGT2_9VIRU Chropara_Vmeth domain-containing protein OS=Wenling tombus-like virus 5 OX=1923547 PE=4 SV=1
MM1 pKa = 7.75PGAKK5 pKa = 9.44PKK7 pKa = 9.05PQRR10 pKa = 11.84VTRR13 pKa = 11.84TPRR16 pKa = 11.84RR17 pKa = 11.84NAQQRR22 pKa = 11.84SVTQQLQSTRR32 pKa = 11.84QVVNLVASNANHH44 pKa = 6.45RR45 pKa = 11.84PVDD48 pKa = 3.74NSTHH52 pKa = 6.59RR53 pKa = 11.84LNGSDD58 pKa = 3.7YY59 pKa = 10.89LGEE62 pKa = 4.27VTTATPLTAKK72 pKa = 9.17NTVRR76 pKa = 11.84LSMQLSPTGFKK87 pKa = 9.19GTRR90 pKa = 11.84VSALGEE96 pKa = 3.94LYY98 pKa = 10.33EE99 pKa = 4.25KK100 pKa = 10.58YY101 pKa = 9.8RR102 pKa = 11.84YY103 pKa = 9.18RR104 pKa = 11.84RR105 pKa = 11.84AVVRR109 pKa = 11.84YY110 pKa = 9.37VPALPATVGGQLLAYY125 pKa = 9.99VDD127 pKa = 4.5QDD129 pKa = 3.65PKK131 pKa = 11.33DD132 pKa = 3.97DD133 pKa = 3.87PRR135 pKa = 11.84EE136 pKa = 4.05VQDD139 pKa = 4.49LKK141 pKa = 11.28ALRR144 pKa = 11.84AMASSSSGAQAWNLPLAKK162 pKa = 9.92SITMPGTNEE171 pKa = 3.86NKK173 pKa = 9.24WYY175 pKa = 8.46YY176 pKa = 9.9TGHH179 pKa = 5.46QEE181 pKa = 4.03EE182 pKa = 4.41NPRR185 pKa = 11.84LSVQGVLHH193 pKa = 6.95LLQMTDD199 pKa = 3.07AVGTDD204 pKa = 3.94GQPLAANSVAGTLYY218 pKa = 10.73LDD220 pKa = 3.41WVLEE224 pKa = 4.35FKK226 pKa = 9.99TPQMNPEE233 pKa = 4.11LVAAAKK239 pKa = 10.47GYY241 pKa = 10.74VPDD244 pKa = 4.33VPIGPPGGGKK254 pKa = 9.35HH255 pKa = 4.87VTLDD259 pKa = 3.4TRR261 pKa = 11.84KK262 pKa = 10.46LGDD265 pKa = 3.73QASSWIPIPSEE276 pKa = 3.93DD277 pKa = 3.89GFITVRR283 pKa = 11.84NIVGCQPNDD292 pKa = 3.07KK293 pKa = 10.91LMEE296 pKa = 4.64HH297 pKa = 7.04KK298 pKa = 11.15GNGFWIGVYY307 pKa = 8.94WSEE310 pKa = 4.12TGSYY314 pKa = 10.78SDD316 pKa = 5.84DD317 pKa = 3.66PLANPIWRR325 pKa = 11.84VKK327 pKa = 10.26YY328 pKa = 10.15INHH331 pKa = 7.09PLSGDD336 pKa = 2.82MWTSWFYY343 pKa = 11.31LADD346 pKa = 5.25DD347 pKa = 5.11DD348 pKa = 6.09SSMSPFIPPANAKK361 pKa = 9.64YY362 pKa = 8.95VTLIEE367 pKa = 4.41STDD370 pKa = 3.97FPSGSEE376 pKa = 4.26VPVDD380 pKa = 3.78PVDD383 pKa = 3.53TPLYY387 pKa = 7.95TGCRR391 pKa = 11.84VDD393 pKa = 4.91IMWTHH398 pKa = 5.45VKK400 pKa = 10.65EE401 pKa = 4.28EE402 pKa = 3.9TDD404 pKa = 3.54LLVRR408 pKa = 11.84RR409 pKa = 4.71

Molecular weight: 45.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGZ0|A0A1L3KGZ0_9VIRU RNA-directed RNA polymerase OS=Wenling tombus-like virus 5 OX=1923547 PE=4 SV=1
MM1 pKa = 7.69LPQILLTTLLTMLPGLAPMPEE22 pKa = 3.9LSTYY26 pKa = 9.47EE27 pKa = 3.95WLGLPGLRR35 pKa = 11.84TVRR38 pKa = 11.84HH39 pKa = 5.05VCEE42 pKa = 4.5SSPLPAALHH51 pKa = 5.15WVVSNTLIDD60 pKa = 3.75QDD62 pKa = 3.48RR63 pKa = 11.84LEE65 pKa = 4.5YY66 pKa = 9.71LTAACEE72 pKa = 3.83RR73 pKa = 11.84RR74 pKa = 11.84ADD76 pKa = 3.62ARR78 pKa = 11.84ASLHH82 pKa = 6.58ALTANRR88 pKa = 11.84LWVGTVPEE96 pKa = 4.94LPHH99 pKa = 6.03VCARR103 pKa = 11.84LNSRR107 pKa = 11.84NGYY110 pKa = 9.08IGVPHH115 pKa = 6.42VVIRR119 pKa = 11.84WLEE122 pKa = 3.96SAQLVLATLPSLFLVGGLLSTALFTCIAVAIVRR155 pKa = 11.84YY156 pKa = 9.67RR157 pKa = 11.84CTAHH161 pKa = 6.34VQVACQSPPYY171 pKa = 8.12THH173 pKa = 6.78RR174 pKa = 11.84QVGQMITQRR183 pKa = 11.84LGTWAPCASGDD194 pKa = 3.82NLHH197 pKa = 6.48GHH199 pKa = 6.92LADD202 pKa = 3.66VRR204 pKa = 11.84RR205 pKa = 11.84TLEE208 pKa = 4.04GACFHH213 pKa = 5.85VLKK216 pKa = 10.68SLTGSAGRR224 pKa = 11.84LRR226 pKa = 11.84DD227 pKa = 3.21IGGSLTRR234 pKa = 11.84NARR237 pKa = 11.84LGKK240 pKa = 9.6KK241 pKa = 9.63LHH243 pKa = 6.04VCFPCLTTADD253 pKa = 3.69AARR256 pKa = 11.84LHH258 pKa = 6.39GAQTSNDD265 pKa = 3.74VGHH268 pKa = 6.7HH269 pKa = 6.29TGQDD273 pKa = 3.66CPVSHH278 pKa = 7.15LPSIMTYY285 pKa = 10.64VDD287 pKa = 4.1FHH289 pKa = 5.92MTPSQLVKK297 pKa = 10.47AIKK300 pKa = 10.47SPTLIVTHH308 pKa = 6.83DD309 pKa = 4.09FARR312 pKa = 11.84VGDD315 pKa = 3.88RR316 pKa = 11.84EE317 pKa = 4.3SWFGDD322 pKa = 3.22EE323 pKa = 5.78AIVEE327 pKa = 4.39KK328 pKa = 10.62FGSSVTMTVKK338 pKa = 10.71GGFTYY343 pKa = 9.49THH345 pKa = 7.54GYY347 pKa = 9.28HH348 pKa = 5.77SWGAEE353 pKa = 3.78GTVCTRR359 pKa = 11.84DD360 pKa = 2.91GAFRR364 pKa = 11.84YY365 pKa = 7.62TRR367 pKa = 11.84VYY369 pKa = 10.9DD370 pKa = 3.51NHH372 pKa = 7.2HH373 pKa = 6.74SVILWCCPLAGSFTPSRR390 pKa = 11.84DD391 pKa = 3.39NEE393 pKa = 4.3LVSSVGVVDD402 pKa = 5.45HH403 pKa = 6.5LVCGDD408 pKa = 3.57GTNAAVSGGKK418 pKa = 9.67YY419 pKa = 9.59VFQAGNRR426 pKa = 11.84TLGTVLASTIVRR438 pKa = 11.84VAYY441 pKa = 10.58QMATGARR448 pKa = 11.84DD449 pKa = 3.94EE450 pKa = 4.47KK451 pKa = 10.65WLSNLRR457 pKa = 11.84NLLRR461 pKa = 11.84GRR463 pKa = 11.84FTADD467 pKa = 3.02KK468 pKa = 10.44QDD470 pKa = 3.85EE471 pKa = 4.4NLLPHH476 pKa = 6.77AADD479 pKa = 3.35VCVQLADD486 pKa = 4.34RR487 pKa = 11.84MGTTYY492 pKa = 10.63TNSVLGDD499 pKa = 3.46PSSYY503 pKa = 11.24SFIVRR508 pKa = 11.84PFVRR512 pKa = 11.84YY513 pKa = 8.57AAAAWHH519 pKa = 6.41LVPAPVAACLRR530 pKa = 11.84WATTCVAQVTYY541 pKa = 10.8VRR543 pKa = 11.84SPLTAWAWSTVNVPSYY559 pKa = 10.99EE560 pKa = 4.3VFWDD564 pKa = 3.49QLATVNPSRR573 pKa = 11.84PVPGPFPATGAGSSAAPAQQPHH595 pKa = 6.41SLPGKK600 pKa = 10.06DD601 pKa = 4.22DD602 pKa = 3.89GASGSRR608 pKa = 11.84DD609 pKa = 3.66PEE611 pKa = 4.06KK612 pKa = 10.64SSCGSTPAAPSTGGQPKK629 pKa = 9.0PAAALPPQPLATPPIPLPRR648 pKa = 11.84TTFPAVTPVASKK660 pKa = 10.57SGQDD664 pKa = 3.07SGAAAQAPRR673 pKa = 11.84DD674 pKa = 4.04DD675 pKa = 4.04KK676 pKa = 11.44RR677 pKa = 11.84GVSAGGSKK685 pKa = 8.78GTKK688 pKa = 9.32RR689 pKa = 11.84PVSIPLPPHH698 pKa = 6.1PSEE701 pKa = 4.08TAGARR706 pKa = 11.84LPPKK710 pKa = 10.07ARR712 pKa = 11.84EE713 pKa = 3.92RR714 pKa = 11.84VAKK717 pKa = 10.5GPAANAVRR725 pKa = 11.84PVGVPVQPVKK735 pKa = 10.21TDD737 pKa = 3.35PAQRR741 pKa = 11.84RR742 pKa = 11.84TDD744 pKa = 3.62PSPTTGAIPKK754 pKa = 9.54RR755 pKa = 11.84RR756 pKa = 11.84RR757 pKa = 11.84NEE759 pKa = 3.83GLHH762 pKa = 6.09QDD764 pKa = 3.43GDD766 pKa = 4.05LTKK769 pKa = 11.14GNGSAQHH776 pKa = 5.37QSKK779 pKa = 9.97AAPVSSGSGAPRR791 pKa = 11.84GQPGKK796 pKa = 10.64GSRR799 pKa = 11.84KK800 pKa = 9.6KK801 pKa = 9.93PLSGKK806 pKa = 9.58GANTNRR812 pKa = 11.84KK813 pKa = 8.24GAQAGALPARR823 pKa = 11.84RR824 pKa = 11.84RR825 pKa = 11.84DD826 pKa = 3.34

Molecular weight: 87.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1634

399

826

544.7

59.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.792 ± 1.139

1.958 ± 0.505

4.957 ± 0.502

3.305 ± 0.629

2.693 ± 0.75

7.895 ± 0.781

3.06 ± 0.375

3.06 ± 0.772

3.55 ± 0.299

8.813 ± 0.096

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.53 ± 0.344

2.938 ± 0.61

8.017 ± 0.568

3.978 ± 0.26

6.916 ± 0.457

7.344 ± 0.196

7.528 ± 0.3

8.262 ± 0.111

1.775 ± 0.248

2.632 ± 0.467
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski