Chryseobacterium sp. 3008163
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3789 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G2G9Q3|A0A3G2G9Q3_9FLAO Thioredoxin family protein OS=Chryseobacterium sp. 3008163 OX=2478663 GN=EAG08_10760 PE=4 SV=1
MM1 pKa = 7.39 NSYY4 pKa = 9.92 IKK6 pKa = 9.86 KK7 pKa = 10.11 IKK9 pKa = 8.71 MKK11 pKa = 10.66 KK12 pKa = 10.21 NIFKK16 pKa = 10.52 YY17 pKa = 10.41 VLVPTMILCAFGINFFKK34 pKa = 10.39 AQNWTTVWTEE44 pKa = 3.66 NFNGLSYY51 pKa = 10.9 SSSYY55 pKa = 10.77 NNGVTGYY62 pKa = 9.34 WNNSANPPQNSQINGAFEE80 pKa = 4.97 ANPANNIVAGTTQVPSDD97 pKa = 3.43 AAGGRR102 pKa = 11.84 FLMFWTQGGVAIPASEE118 pKa = 3.71 NVFFKK123 pKa = 10.22 KK124 pKa = 9.36 TMTVVPGKK132 pKa = 8.39 TYY134 pKa = 10.29 RR135 pKa = 11.84 INYY138 pKa = 8.99 RR139 pKa = 11.84 FATLGAVPGTATNRR153 pKa = 11.84 ANATFRR159 pKa = 11.84 VSPQGNTTSFYY170 pKa = 8.76 TSGMVVADD178 pKa = 3.32 VNAWKK183 pKa = 10.35 NVQYY187 pKa = 10.67 EE188 pKa = 4.15 FTVPSSVTSVDD199 pKa = 3.61 MTWEE203 pKa = 4.07 NTTKK207 pKa = 9.8 STTGNDD213 pKa = 3.23 FALDD217 pKa = 3.75 DD218 pKa = 4.61 LVLQEE223 pKa = 5.55 AVDD226 pKa = 3.93 SDD228 pKa = 4.03 GDD230 pKa = 4.26 GIPDD234 pKa = 4.85 YY235 pKa = 11.2 IDD237 pKa = 6.02 DD238 pKa = 5.7 DD239 pKa = 4.86 DD240 pKa = 6.7 DD241 pKa = 5.72 NDD243 pKa = 5.25 GILDD247 pKa = 3.72 TAEE250 pKa = 4.44 CGGGAIYY257 pKa = 10.22 NYY259 pKa = 10.23 SGFGIRR265 pKa = 11.84 TISATQANLIGLTGNTYY282 pKa = 9.93 TPTTIATNIIPGNDD296 pKa = 3.76 ANHH299 pKa = 5.76 MTTDD303 pKa = 3.48 AARR306 pKa = 11.84 NRR308 pKa = 11.84 VLFANNIAQGSLFAYY323 pKa = 9.55 QFSTNTVVNVGSGFLSSLGDD343 pKa = 3.62 ASGGAAMYY351 pKa = 10.92 NNDD354 pKa = 3.19 YY355 pKa = 10.8 FIYY358 pKa = 10.15 DD359 pKa = 3.65 DD360 pKa = 4.52 TGTTSQGLWRR370 pKa = 11.84 VTFNASGNASTLTKK384 pKa = 10.54 VVDD387 pKa = 4.85 PISGIDD393 pKa = 3.86 LGDD396 pKa = 3.25 IAINSSGMVYY406 pKa = 10.56 VIGSTGALYY415 pKa = 10.86 KK416 pKa = 10.55 LDD418 pKa = 4.24 LSSLNLSITTPSSAWISVGTTASASGTQLFFGANGDD454 pKa = 4.44 LIGSNSGNLIRR465 pKa = 11.84 IDD467 pKa = 3.78 PNNAANLGTISTLTSGYY484 pKa = 9.18 SWGDD488 pKa = 2.92 ISEE491 pKa = 4.36 APSVGFSCGTDD502 pKa = 3.13 TDD504 pKa = 4.16 SDD506 pKa = 4.6 GIPNNLDD513 pKa = 3.53 LDD515 pKa = 4.21 SDD517 pKa = 4.41 GDD519 pKa = 3.94 GCPDD523 pKa = 3.19 ARR525 pKa = 11.84 EE526 pKa = 4.1 GAGNFNPTTIASGTIASQTPNTNFGTAVSVTTGVPTAVGAGQSVGQSQDD575 pKa = 2.75 SSKK578 pKa = 11.24 NDD580 pKa = 3.64 CLDD583 pKa = 3.32 SDD585 pKa = 5.08 GDD587 pKa = 5.0 GLPDD591 pKa = 3.7 WQDD594 pKa = 3.56 LDD596 pKa = 4.64 DD597 pKa = 6.11 DD598 pKa = 4.68 NDD600 pKa = 5.0 GILDD604 pKa = 3.97 TVEE607 pKa = 4.12 CTPTYY612 pKa = 10.36 LVRR615 pKa = 11.84 PVTSSSVTADD625 pKa = 3.18 KK626 pKa = 10.73 PITVGTAPQIADD638 pKa = 3.55 GEE640 pKa = 4.76 GAGGSGDD647 pKa = 3.69 GPFPYY652 pKa = 9.71 WYY654 pKa = 9.14 TNVPNLPIAFSMNMQSSSTIDD675 pKa = 3.22 HH676 pKa = 6.16 VKK678 pKa = 10.65 LYY680 pKa = 10.71 GPWGFNEE687 pKa = 4.35 WIGNFTIEE695 pKa = 5.21 LYY697 pKa = 10.8 NAGNTLLGTEE707 pKa = 4.43 NFVAPDD713 pKa = 3.69 QYY715 pKa = 10.88 TGTPIFSFTKK725 pKa = 9.8 EE726 pKa = 3.9 YY727 pKa = 10.73 TNVARR732 pKa = 11.84 VRR734 pKa = 11.84 FTIVSSQGYY743 pKa = 7.73 STVTPPRR750 pKa = 11.84 ASVNEE755 pKa = 3.9 IVFLDD760 pKa = 4.29 LQPLTCDD767 pKa = 3.61 TDD769 pKa = 3.56 NDD771 pKa = 5.2 GIPNHH776 pKa = 7.09 LDD778 pKa = 3.34 LDD780 pKa = 4.19 SDD782 pKa = 4.4 NDD784 pKa = 3.88 GCLDD788 pKa = 4.14 AMEE791 pKa = 5.03 GDD793 pKa = 4.01 EE794 pKa = 4.19 NVTVNMLVNAASGLSVGTGSSASNQNLCASGTCVNANGVPTQVNSGGAADD844 pKa = 3.6 IGGDD848 pKa = 3.43 VGQGIGDD855 pKa = 3.89 SQNALISSGCYY866 pKa = 8.82 CYY868 pKa = 10.6 KK869 pKa = 10.43 PEE871 pKa = 4.22 VVVAGNPNPVKK882 pKa = 10.7 HH883 pKa = 6.72 GITALKK889 pKa = 9.5 RR890 pKa = 11.84 ANSGTSDD897 pKa = 3.32 WPVVRR902 pKa = 11.84 QSAWTVLEE910 pKa = 4.27 SKK912 pKa = 9.68 TKK914 pKa = 10.27 GFVLNRR920 pKa = 11.84 MAFVDD925 pKa = 3.9 ADD927 pKa = 4.29 SNTATPTTPPLASIPAANYY946 pKa = 8.55 VEE948 pKa = 4.61 GMMVYY953 pKa = 9.04 DD954 pKa = 4.7 TVAKK958 pKa = 10.09 CLKK961 pKa = 9.76 IYY963 pKa = 10.91 NGTIWSCFSTQTCPDD978 pKa = 3.14
Molecular weight: 102.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.751
IPC2_protein 3.935
IPC_protein 3.986
Toseland 3.745
ProMoST 4.139
Dawson 3.986
Bjellqvist 4.151
Wikipedia 3.948
Rodwell 3.808
Grimsley 3.643
Solomon 3.986
Lehninger 3.948
Nozaki 4.088
DTASelect 4.406
Thurlkill 3.795
EMBOSS 3.948
Sillero 4.113
Patrickios 1.074
IPC_peptide 3.973
IPC2_peptide 4.088
IPC2.peptide.svr19 3.978
Protein with the highest isoelectric point:
>tr|A0A3G2G9K5|A0A3G2G9K5_9FLAO Uncharacterized protein OS=Chryseobacterium sp. 3008163 OX=2478663 GN=EAG08_13650 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPSEE10 pKa = 3.58 RR11 pKa = 11.84 KK12 pKa = 9.37 KK13 pKa = 10.2 RR14 pKa = 11.84 NKK16 pKa = 9.71 HH17 pKa = 4.09 GFRR20 pKa = 11.84 EE21 pKa = 4.33 RR22 pKa = 11.84 MSTPNGRR29 pKa = 11.84 RR30 pKa = 11.84 VLAARR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 9.98 GRR40 pKa = 11.84 KK41 pKa = 8.81 SLTVSSARR49 pKa = 11.84 AKK51 pKa = 10.4 RR52 pKa = 3.45
Molecular weight: 6.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 10.745
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.486
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.223
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.959
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.101
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3789
0
3789
1171329
25
2833
309.1
34.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.004 ± 0.04
0.729 ± 0.012
5.403 ± 0.03
6.56 ± 0.05
5.52 ± 0.034
6.233 ± 0.044
1.637 ± 0.016
8.035 ± 0.043
8.404 ± 0.046
8.966 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.315 ± 0.02
6.677 ± 0.049
3.329 ± 0.022
3.53 ± 0.024
3.069 ± 0.024
6.765 ± 0.032
5.653 ± 0.044
5.933 ± 0.031
1.061 ± 0.016
4.178 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here