Streptococcus phage Javan84

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6BF99|A0A4D6BF99_9CAUD NYN domain-containing protein OS=Streptococcus phage Javan84 OX=2548309 GN=Javan84_0057 PE=4 SV=1
MM1 pKa = 7.72PEE3 pKa = 4.14IYY5 pKa = 10.14QISLVDD11 pKa = 3.65YY12 pKa = 10.9QMEE15 pKa = 4.15LALLVLFDD23 pKa = 4.51YY24 pKa = 10.19YY25 pKa = 10.93TDD27 pKa = 3.58NVLVSSPHH35 pKa = 6.13PLSVVV40 pKa = 3.06

Molecular weight: 4.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6BEQ9|A0A4D6BEQ9_9CAUD Uncharacterized protein OS=Streptococcus phage Javan84 OX=2548309 GN=Javan84_0003 PE=4 SV=1
MM1 pKa = 7.48ASYY4 pKa = 10.53RR5 pKa = 11.84KK6 pKa = 9.31RR7 pKa = 11.84DD8 pKa = 3.51GSWEE12 pKa = 3.63YY13 pKa = 10.59RR14 pKa = 11.84ISYY17 pKa = 7.39KK18 pKa = 10.53TPDD21 pKa = 3.22GKK23 pKa = 11.03YY24 pKa = 8.55KK25 pKa = 10.48QKK27 pKa = 10.61SKK29 pKa = 10.98RR30 pKa = 11.84GFRR33 pKa = 11.84TKK35 pKa = 10.63KK36 pKa = 9.27EE37 pKa = 4.06AEE39 pKa = 3.95LAAAEE44 pKa = 4.39AEE46 pKa = 4.0RR47 pKa = 11.84LLNSTTIFDD56 pKa = 4.39EE57 pKa = 4.74NISLYY62 pKa = 10.79DD63 pKa = 4.67YY64 pKa = 10.37FDD66 pKa = 3.4KK67 pKa = 10.42WAKK70 pKa = 9.78IYY72 pKa = 10.1KK73 pKa = 9.15KK74 pKa = 8.83PHH76 pKa = 5.04VSIGTWKK83 pKa = 10.91NYY85 pKa = 10.35DD86 pKa = 3.36QTLKK90 pKa = 10.88LIEE93 pKa = 4.36RR94 pKa = 11.84EE95 pKa = 3.89FGQTKK100 pKa = 9.46IRR102 pKa = 11.84AITPSIYY109 pKa = 10.09QQFLNNLGKK118 pKa = 10.2RR119 pKa = 11.84YY120 pKa = 9.34YY121 pKa = 10.17QGTIHH126 pKa = 6.85KK127 pKa = 8.32VHH129 pKa = 5.99HH130 pKa = 6.73RR131 pKa = 11.84IRR133 pKa = 11.84RR134 pKa = 11.84AVKK137 pKa = 8.6QAVVEE142 pKa = 4.23KK143 pKa = 10.91LIDD146 pKa = 3.59TNFTDD151 pKa = 3.88LAKK154 pKa = 10.52INAEE158 pKa = 4.0RR159 pKa = 11.84QHH161 pKa = 7.63KK162 pKa = 9.66PIEE165 pKa = 4.44DD166 pKa = 3.4KK167 pKa = 10.89FLEE170 pKa = 4.31EE171 pKa = 4.49NEE173 pKa = 4.16YY174 pKa = 11.22LSLLTTLKK182 pKa = 10.53QNCPQTDD189 pKa = 3.87YY190 pKa = 11.32VQLYY194 pKa = 8.69LLSVTGMRR202 pKa = 11.84IGEE205 pKa = 4.3SLGLTWNDD213 pKa = 2.33IDD215 pKa = 5.06FKK217 pKa = 11.5NGLININKK225 pKa = 7.28TWNVYY230 pKa = 8.84TNAEE234 pKa = 3.98FAPTKK239 pKa = 10.13NKK241 pKa = 10.26QSMRR245 pKa = 11.84TIPLDD250 pKa = 3.22NTTAKK255 pKa = 10.33ILLQFKK261 pKa = 8.92TEE263 pKa = 3.74EE264 pKa = 4.18WTVNPYY270 pKa = 10.07NRR272 pKa = 11.84LFIRR276 pKa = 11.84VNHH279 pKa = 6.34PWLNRR284 pKa = 11.84LIKK287 pKa = 10.55KK288 pKa = 7.59LTKK291 pKa = 10.26TNIHH295 pKa = 4.95VHH297 pKa = 5.1SLRR300 pKa = 11.84HH301 pKa = 5.46TYY303 pKa = 10.81ASYY306 pKa = 10.88LISRR310 pKa = 11.84QIDD313 pKa = 3.81LLTISNLLGHH323 pKa = 7.16KK324 pKa = 10.21DD325 pKa = 3.46LTVTLQTYY333 pKa = 9.12AHH335 pKa = 6.13QLEE338 pKa = 4.39QQKK341 pKa = 10.67EE342 pKa = 3.95KK343 pKa = 11.06DD344 pKa = 3.35FKK346 pKa = 10.85EE347 pKa = 3.89IKK349 pKa = 10.28KK350 pKa = 10.66LFGG353 pKa = 3.74

Molecular weight: 41.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

13199

40

1271

227.6

25.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.97 ± 0.756

0.568 ± 0.102

6.076 ± 0.351

7.16 ± 0.549

3.781 ± 0.178

6.447 ± 0.402

1.614 ± 0.152

6.985 ± 0.279

7.448 ± 0.491

8.129 ± 0.299

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.705 ± 0.208

4.909 ± 0.318

3.068 ± 0.201

4.25 ± 0.296

4.493 ± 0.258

6.372 ± 0.454

6.213 ± 0.35

6.788 ± 0.396

1.197 ± 0.165

3.826 ± 0.348
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski