Methyloceanibacter superfactus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2686 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E3W2I8|A0A1E3W2I8_9RHIZ Uncharacterized protein OS=Methyloceanibacter superfactus OX=1774969 GN=AUC69_07995 PE=4 SV=1
MM1 pKa = 7.51LGAGLAALSLTAVALVPATAKK22 pKa = 9.99TGEE25 pKa = 4.34SNHH28 pKa = 6.78DD29 pKa = 3.62KK30 pKa = 10.93AIQACSTVNPAQPVEE45 pKa = 4.55AIGAVDD51 pKa = 4.33DD52 pKa = 4.33GSGIGFSLVWITDD65 pKa = 3.5SEE67 pKa = 4.27DD68 pKa = 3.69RR69 pKa = 11.84LWMCDD74 pKa = 3.07ADD76 pKa = 4.28SEE78 pKa = 5.02GFVYY82 pKa = 10.34SYY84 pKa = 11.77SLVNDD89 pKa = 4.48DD90 pKa = 4.85LLEE93 pKa = 4.48GAGPEE98 pKa = 4.64LIGLKK103 pKa = 9.58PASNGGYY110 pKa = 9.36EE111 pKa = 4.27DD112 pKa = 3.58QPQTVAEE119 pKa = 4.76TICAAYY125 pKa = 8.67LTEE128 pKa = 4.87GGTVLSSRR136 pKa = 11.84PDD138 pKa = 3.5GLSEE142 pKa = 4.22DD143 pKa = 3.15PGYY146 pKa = 11.41AVFVEE151 pKa = 4.57DD152 pKa = 5.37KK153 pKa = 11.2GGALYY158 pKa = 10.62LCNATGDD165 pKa = 3.57AMVWAFEE172 pKa = 4.64PIGDD176 pKa = 4.24PLTFGKK182 pKa = 8.87TEE184 pKa = 3.88AVSS187 pKa = 3.24

Molecular weight: 19.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E3VWA9|A0A1E3VWA9_9RHIZ Uncharacterized protein OS=Methyloceanibacter superfactus OX=1774969 GN=AUC69_01470 PE=3 SV=1
MM1 pKa = 7.06VKK3 pKa = 10.0KK4 pKa = 10.16SKK6 pKa = 10.26SGRR9 pKa = 11.84PVAKK13 pKa = 9.79TSAKK17 pKa = 10.09RR18 pKa = 11.84KK19 pKa = 7.53TSAKK23 pKa = 9.77RR24 pKa = 11.84KK25 pKa = 9.6APAKK29 pKa = 9.19KK30 pKa = 7.9TAAKK34 pKa = 10.25AKK36 pKa = 8.75TAAKK40 pKa = 9.71RR41 pKa = 11.84KK42 pKa = 9.01LAAKK46 pKa = 9.56PVAKK50 pKa = 10.39KK51 pKa = 9.1KK52 pKa = 7.08TTAKK56 pKa = 10.11RR57 pKa = 11.84QVTAKK62 pKa = 9.21TAAKK66 pKa = 9.63RR67 pKa = 11.84KK68 pKa = 8.33PAVKK72 pKa = 10.23SKK74 pKa = 9.82VARR77 pKa = 11.84KK78 pKa = 7.42TVTPKK83 pKa = 10.3KK84 pKa = 9.89AAPKK88 pKa = 10.08KK89 pKa = 10.0GLAKK93 pKa = 10.4KK94 pKa = 8.39EE95 pKa = 3.83LRR97 pKa = 11.84RR98 pKa = 11.84KK99 pKa = 9.88GSAPRR104 pKa = 11.84RR105 pKa = 11.84RR106 pKa = 11.84LRR108 pKa = 11.84RR109 pKa = 11.84RR110 pKa = 11.84RR111 pKa = 11.84LRR113 pKa = 11.84SGQRR117 pKa = 11.84RR118 pKa = 11.84SGAPRR123 pKa = 11.84RR124 pKa = 11.84VRR126 pKa = 11.84RR127 pKa = 11.84RR128 pKa = 11.84NPRR131 pKa = 11.84RR132 pKa = 11.84SPPRR136 pKa = 11.84RR137 pKa = 11.84LPPKK141 pKa = 10.37NRR143 pKa = 3.42

Molecular weight: 16.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2686

0

2686

721614

29

1950

268.7

29.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.462 ± 0.072

0.933 ± 0.02

5.825 ± 0.042

6.001 ± 0.047

3.65 ± 0.033

8.513 ± 0.044

2.031 ± 0.026

4.934 ± 0.035

3.826 ± 0.043

10.155 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.359 ± 0.024

2.439 ± 0.026

5.406 ± 0.038

2.979 ± 0.03

6.917 ± 0.05

5.237 ± 0.031

5.236 ± 0.032

7.426 ± 0.045

1.334 ± 0.02

2.337 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski