Reichenbachiella faecimaris
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3972 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W2GHE7|A0A1W2GHE7_9BACT Antitoxin component YwqK of the YwqJK toxin-antitoxin module OS=Reichenbachiella faecimaris OX=692418 GN=SAMN04488029_2702 PE=4 SV=1
MM1 pKa = 7.67 KK2 pKa = 10.33 NFKK5 pKa = 10.1 IYY7 pKa = 9.87 TLILVSACIAFSCDD21 pKa = 3.51 EE22 pKa = 4.22 EE23 pKa = 5.65 DD24 pKa = 5.54 KK25 pKa = 11.34 LITDD29 pKa = 4.56 ILTEE33 pKa = 4.2 NPLPVPTGDD42 pKa = 3.9 PGEE45 pKa = 5.02 LDD47 pKa = 3.33 LSTYY51 pKa = 9.86 VAIGNSLTAGFQDD64 pKa = 3.1 NALYY68 pKa = 10.29 TDD70 pKa = 3.8 GQINAFPAYY79 pKa = 9.0 LTTQFSAQGVGGGDD93 pKa = 3.39 YY94 pKa = 10.66 VYY96 pKa = 11.0 PDD98 pKa = 3.31 INSEE102 pKa = 4.0 NGYY105 pKa = 10.49 SGTDD109 pKa = 3.33 GTNLFGKK116 pKa = 10.3 YY117 pKa = 9.34 ILNITEE123 pKa = 4.2 LGIEE127 pKa = 4.25 ISAGEE132 pKa = 3.99 NNIGLRR138 pKa = 11.84 ATEE141 pKa = 4.53 DD142 pKa = 3.57 EE143 pKa = 5.11 IEE145 pKa = 4.12 ALTNFGVPGATISDD159 pKa = 3.99 LDD161 pKa = 4.1 DD162 pKa = 4.37 ASFANPYY169 pKa = 9.91 FLRR172 pKa = 11.84 FSSNPLNVSILDD184 pKa = 3.61 DD185 pKa = 3.72 VVARR189 pKa = 11.84 NPTFISLWIGSNDD202 pKa = 3.4 YY203 pKa = 10.94 LGYY206 pKa = 10.99 ALGGAASGAPTDD218 pKa = 3.52 VATFQTMFTNHH229 pKa = 4.57 MTKK232 pKa = 10.59 LAGTGAKK239 pKa = 9.88 GVVLNLPPVTLIPYY253 pKa = 9.21 FRR255 pKa = 11.84 AVPYY259 pKa = 10.44 NAVPLTDD266 pKa = 3.64 QSDD269 pKa = 3.82 VDD271 pKa = 4.39 DD272 pKa = 5.54 LNAATAFGGYY282 pKa = 8.75 NAALDD287 pKa = 5.17 GIAAMDD293 pKa = 4.32 PNFSQEE299 pKa = 4.1 DD300 pKa = 3.43 ADD302 pKa = 4.03 EE303 pKa = 4.81 RR304 pKa = 11.84 KK305 pKa = 8.33 VTYY308 pKa = 10.51 SLGANPVLIKK318 pKa = 10.54 DD319 pKa = 3.67 TDD321 pKa = 4.11 LVDD324 pKa = 4.02 LTTTLAGINPALALYY339 pKa = 9.5 GQTRR343 pKa = 11.84 QANEE347 pKa = 3.66 NDD349 pKa = 4.91 LILLPASTLIGTDD362 pKa = 3.39 AEE364 pKa = 4.47 GLPDD368 pKa = 3.88 VPIGVANPLGDD379 pKa = 3.78 EE380 pKa = 4.3 YY381 pKa = 11.85 VLTFSEE387 pKa = 4.33 QVEE390 pKa = 4.94 VITARR395 pKa = 11.84 ATFNGVISAVLANSAFSNFKK415 pKa = 10.65 LVDD418 pKa = 3.51 IQPFFADD425 pKa = 3.64 AFGLSAAQATALALTSDD442 pKa = 5.13 AIAAADD448 pKa = 3.65 GVLGIEE454 pKa = 4.31 YY455 pKa = 10.31 QGFDD459 pKa = 3.81 YY460 pKa = 11.37 SPDD463 pKa = 3.5 FTPNGLWSNDD473 pKa = 2.91 GVHH476 pKa = 6.63 PNPRR480 pKa = 11.84 GHH482 pKa = 7.25 AIVANEE488 pKa = 4.21 IIEE491 pKa = 4.43 TMNEE495 pKa = 3.41 TWSADD500 pKa = 3.14 IPTVTVNQKK509 pKa = 9.59 RR510 pKa = 11.84 ASPFTQQ516 pKa = 3.08
Molecular weight: 54.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.681
IPC2_protein 3.732
IPC_protein 3.77
Toseland 3.541
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.706
Rodwell 3.592
Grimsley 3.439
Solomon 3.757
Lehninger 3.719
Nozaki 3.872
DTASelect 4.126
Thurlkill 3.592
EMBOSS 3.706
Sillero 3.884
Patrickios 1.329
IPC_peptide 3.757
IPC2_peptide 3.859
IPC2.peptide.svr19 3.798
Protein with the highest isoelectric point:
>tr|A0A1W2GMQ2|A0A1W2GMQ2_9BACT 3-oxoacyl-[acyl-carrier-protein] reductase OS=Reichenbachiella faecimaris OX=692418 GN=SAMN04488029_3551 PE=4 SV=1
MM1 pKa = 7.57 KK2 pKa = 10.5 GIQEE6 pKa = 4.23 FFEE9 pKa = 4.66 KK10 pKa = 10.83 YY11 pKa = 10.66 AFGVCTRR18 pKa = 11.84 LGEE21 pKa = 4.23 KK22 pKa = 10.34 LRR24 pKa = 11.84 IPSSSIRR31 pKa = 11.84 LFFIYY36 pKa = 10.14 TSFLTFGSPVLVYY49 pKa = 11.01 LMLAFVMNFRR59 pKa = 11.84 KK60 pKa = 10.04 HH61 pKa = 3.6 IRR63 pKa = 11.84 RR64 pKa = 11.84 RR65 pKa = 11.84 HH66 pKa = 4.71 SLWYY70 pKa = 9.8 HH71 pKa = 6.01
Molecular weight: 8.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.317
IPC2_protein 9.97
IPC_protein 10.701
Toseland 10.54
ProMoST 10.292
Dawson 10.701
Bjellqvist 10.438
Wikipedia 10.921
Rodwell 10.965
Grimsley 10.789
Solomon 10.774
Lehninger 10.745
Nozaki 10.526
DTASelect 10.423
Thurlkill 10.57
EMBOSS 10.935
Sillero 10.628
Patrickios 10.716
IPC_peptide 10.774
IPC2_peptide 9.458
IPC2.peptide.svr19 8.273
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3972
0
3972
1432065
29
9392
360.5
40.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.695 ± 0.039
0.749 ± 0.012
5.946 ± 0.053
6.835 ± 0.04
5.001 ± 0.029
6.782 ± 0.045
1.919 ± 0.02
7.42 ± 0.035
6.747 ± 0.06
9.453 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.416 ± 0.024
5.456 ± 0.034
3.35 ± 0.021
3.636 ± 0.023
3.593 ± 0.03
6.99 ± 0.035
5.519 ± 0.055
6.328 ± 0.031
1.146 ± 0.016
4.02 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here