Sewage-associated circular DNA virus-26

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4UI48|A0A0B4UI48_9VIRU Replication-associated protein OS=Sewage-associated circular DNA virus-26 OX=1592093 PE=3 SV=1
MM1 pKa = 7.42PKK3 pKa = 9.87KK4 pKa = 10.68GKK6 pKa = 10.56FEE8 pKa = 4.2FRR10 pKa = 11.84TKK12 pKa = 10.98AKK14 pKa = 9.97LHH16 pKa = 6.12SLTYY20 pKa = 7.63PQCDD24 pKa = 3.15IPVDD28 pKa = 4.03FMMAALKK35 pKa = 10.38KK36 pKa = 10.0IAGDD40 pKa = 3.08RR41 pKa = 11.84WGWALIGAEE50 pKa = 4.12DD51 pKa = 3.59HH52 pKa = 7.34AEE54 pKa = 3.9RR55 pKa = 11.84TTDD58 pKa = 3.39EE59 pKa = 4.45FKK61 pKa = 10.15HH62 pKa = 6.35CGVHH66 pKa = 4.41RR67 pKa = 11.84HH68 pKa = 5.52VMQQYY73 pKa = 7.93EE74 pKa = 4.84CKK76 pKa = 10.75SFDD79 pKa = 3.9TVNVRR84 pKa = 11.84YY85 pKa = 9.11WDD87 pKa = 3.68IEE89 pKa = 4.34WNGKK93 pKa = 9.02NYY95 pKa = 9.89HH96 pKa = 5.91PHH98 pKa = 6.33FEE100 pKa = 4.07PVKK103 pKa = 10.59IKK105 pKa = 10.83AKK107 pKa = 8.92WLEE110 pKa = 3.81YY111 pKa = 10.42CMKK114 pKa = 10.05DD115 pKa = 3.15GEE117 pKa = 4.56YY118 pKa = 9.75IYY120 pKa = 10.91EE121 pKa = 4.25GDD123 pKa = 3.83YY124 pKa = 11.36NGAPFDD130 pKa = 4.65PNTYY134 pKa = 9.79FQANKK139 pKa = 10.05SKK141 pKa = 10.66QSYY144 pKa = 8.72GFVWMANEE152 pKa = 3.75IKK154 pKa = 10.5AGKK157 pKa = 7.79TLDD160 pKa = 3.87EE161 pKa = 5.34LDD163 pKa = 4.42DD164 pKa = 4.2LVPGHH169 pKa = 5.9VLQHH173 pKa = 5.35KK174 pKa = 10.15RR175 pKa = 11.84KK176 pKa = 8.74IEE178 pKa = 4.06EE179 pKa = 4.01YY180 pKa = 10.86QEE182 pKa = 3.85FQAKK186 pKa = 9.55KK187 pKa = 10.08KK188 pKa = 9.09EE189 pKa = 3.88RR190 pKa = 11.84RR191 pKa = 11.84TPKK194 pKa = 9.95PIFYY198 pKa = 10.33GFNDD202 pKa = 3.47VEE204 pKa = 4.28DD205 pKa = 4.54PQWQRR210 pKa = 11.84VVKK213 pKa = 9.23WANKK217 pKa = 9.27NFLKK221 pKa = 10.59KK222 pKa = 10.27RR223 pKa = 11.84SSKK226 pKa = 10.41QKK228 pKa = 10.62QLFLWSEE235 pKa = 4.0APNLGKK241 pKa = 7.96THH243 pKa = 7.08PWAEE247 pKa = 4.04TLTEE251 pKa = 4.79FFKK254 pKa = 11.03CYY256 pKa = 9.99NWVNSEE262 pKa = 4.18RR263 pKa = 11.84QGKK266 pKa = 9.53ALMDD270 pKa = 4.13CDD272 pKa = 4.89YY273 pKa = 11.38ILLDD277 pKa = 4.28EE278 pKa = 4.62YY279 pKa = 11.43KK280 pKa = 11.13GMLTITDD287 pKa = 4.56LKK289 pKa = 10.95RR290 pKa = 11.84NSQMMKK296 pKa = 8.49WPVEE300 pKa = 3.83IKK302 pKa = 10.88YY303 pKa = 10.83GDD305 pKa = 3.52STEE308 pKa = 4.13FTRR311 pKa = 11.84NVPMIITSNHH321 pKa = 5.73DD322 pKa = 2.96MRR324 pKa = 11.84SIYY327 pKa = 10.05KK328 pKa = 9.75KK329 pKa = 10.1CDD331 pKa = 3.18YY332 pKa = 10.96RR333 pKa = 11.84DD334 pKa = 3.55IEE336 pKa = 4.29SLEE339 pKa = 3.81VRR341 pKa = 11.84FKK343 pKa = 10.69VVKK346 pKa = 10.54VDD348 pKa = 4.12TVCHH352 pKa = 5.95LTLKK356 pKa = 10.51GPPPVVLVPLPRR368 pKa = 11.84EE369 pKa = 3.99QPTLVAVPEE378 pKa = 3.98NDD380 pKa = 4.36KK381 pKa = 11.37EE382 pKa = 4.15DD383 pKa = 4.03LSDD386 pKa = 3.67EE387 pKa = 4.29EE388 pKa = 5.13EE389 pKa = 4.33ILSSLDD395 pKa = 3.97DD396 pKa = 4.18EE397 pKa = 5.68DD398 pKa = 6.07SEE400 pKa = 4.76FTTTLKK406 pKa = 10.78LAAKK410 pKa = 9.24EE411 pKa = 4.09KK412 pKa = 10.65YY413 pKa = 8.85SVKK416 pKa = 10.68YY417 pKa = 10.24FDD419 pKa = 3.85

Molecular weight: 49.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4UI48|A0A0B4UI48_9VIRU Replication-associated protein OS=Sewage-associated circular DNA virus-26 OX=1592093 PE=3 SV=1
MM1 pKa = 7.58SYY3 pKa = 10.78KK4 pKa = 10.6SKK6 pKa = 10.57LWVGKK11 pKa = 10.19RR12 pKa = 11.84NAPRR16 pKa = 11.84TKK18 pKa = 9.33SQRR21 pKa = 11.84YY22 pKa = 7.86ANQVRR27 pKa = 11.84TNLLRR32 pKa = 11.84KK33 pKa = 9.34IVGNRR38 pKa = 11.84VPRR41 pKa = 11.84PMPMRR46 pKa = 11.84RR47 pKa = 11.84SIKK50 pKa = 9.6TGEE53 pKa = 4.27IKK55 pKa = 10.89CLDD58 pKa = 3.49QTFLATYY65 pKa = 9.14NATWVPDD72 pKa = 3.8AFPQKK77 pKa = 10.65VLNADD82 pKa = 3.54SNGAFQAINLIQQGNGISQRR102 pKa = 11.84IGNKK106 pKa = 9.41INLKK110 pKa = 8.4SVRR113 pKa = 11.84LRR115 pKa = 11.84FSLDD119 pKa = 3.0PTTVTTASPTDD130 pKa = 3.44TRR132 pKa = 11.84VMLVYY137 pKa = 10.47DD138 pKa = 4.2RR139 pKa = 11.84QPNGLYY145 pKa = 10.1PSLNDD150 pKa = 3.33FLNGVSQSNALLNPSGLALCSSNINPNNFEE180 pKa = 4.21RR181 pKa = 11.84FTVLMDD187 pKa = 3.88KK188 pKa = 10.87SITLPSSQASSNFGPTGDD206 pKa = 4.37GQNSPWWIDD215 pKa = 3.46EE216 pKa = 4.28YY217 pKa = 10.82IKK219 pKa = 11.1CKK221 pKa = 10.24GLPVTYY227 pKa = 9.64KK228 pKa = 10.75ANSAPSVIGDD238 pKa = 3.63IATGTLFLFCVGEE251 pKa = 4.09NVALGTSWSYY261 pKa = 10.97RR262 pKa = 11.84GQARR266 pKa = 11.84LRR268 pKa = 11.84YY269 pKa = 9.11YY270 pKa = 10.77DD271 pKa = 3.22AA272 pKa = 6.64

Molecular weight: 30.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

691

272

419

345.5

39.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.499 ± 0.638

1.737 ± 0.152

6.223 ± 1.033

5.644 ± 2.381

4.342 ± 0.379

5.355 ± 0.93

1.737 ± 0.991

4.486 ± 0.377

8.828 ± 2.1

7.959 ± 0.703

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.46 ± 0.355

5.933 ± 1.649

5.644 ± 0.346

4.052 ± 0.415

5.065 ± 0.886

6.223 ± 1.694

5.933 ± 0.6

6.078 ± 0.112

2.46 ± 0.355

4.342 ± 0.379
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski