Chimpanzee associated porprismacovirus 1
Average proteome isoelectric point is 6.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D2WKE3|D2WKE3_9VIRU Putative replicase protein OS=Chimpanzee associated porprismacovirus 1 OX=2170109 GN=rep PE=4 SV=1
MM1 pKa = 7.01 FVKK4 pKa = 10.65 VSEE7 pKa = 4.49 TYY9 pKa = 10.72 DD10 pKa = 4.37 LSTQTDD16 pKa = 2.71 KK17 pKa = 11.16 MGFVGIHH24 pKa = 4.75 TPEE27 pKa = 4.3 GKK29 pKa = 9.88 LVYY32 pKa = 10.76 NMWSGLFKK40 pKa = 10.78 NFRR43 pKa = 11.84 KK44 pKa = 9.39 FRR46 pKa = 11.84 YY47 pKa = 8.92 ASCDD51 pKa = 3.08 VTMACASMLPADD63 pKa = 4.26 PLQIGVEE70 pKa = 4.09 AGDD73 pKa = 4.21 IAPQDD78 pKa = 3.63 MFNPILYY85 pKa = 8.86 KK86 pKa = 10.64 ACSNDD91 pKa = 3.26 SMSVLLNRR99 pKa = 11.84 LYY101 pKa = 11.07 AGADD105 pKa = 3.19 ASTSAINKK113 pKa = 9.21 NSVVAQNDD121 pKa = 3.17 EE122 pKa = 4.44 SFGYY126 pKa = 10.62 DD127 pKa = 3.07 ADD129 pKa = 4.02 NDD131 pKa = 3.39 VDD133 pKa = 4.06 QFAMYY138 pKa = 10.28 YY139 pKa = 10.82 GLLADD144 pKa = 3.49 SDD146 pKa = 4.19 GWRR149 pKa = 11.84 KK150 pKa = 10.0 AMPQAGLQMHH160 pKa = 6.13 GRR162 pKa = 11.84 KK163 pKa = 8.97 PLVFGITSNFGQPSNVGLPNGKK185 pKa = 8.15 MVYY188 pKa = 9.01 TGNGDD193 pKa = 3.34 GSLTARR199 pKa = 11.84 NTIDD203 pKa = 3.26 STLFQYY209 pKa = 9.91 MRR211 pKa = 11.84 GPTMGMPALDD221 pKa = 3.54 TFVQLGGSTVVNGFADD237 pKa = 3.74 SVTVPEE243 pKa = 4.4 VSDD246 pKa = 3.29 RR247 pKa = 11.84 AYY249 pKa = 9.87 PISNGSITTPGNVCTPNLDD268 pKa = 3.78 TPDD271 pKa = 3.94 CFVAAIVLPPAKK283 pKa = 10.22 LNRR286 pKa = 11.84 LYY288 pKa = 11.4 YY289 pKa = 10.02 RR290 pKa = 11.84 MKK292 pKa = 9.55 VTWTIEE298 pKa = 4.11 FSGLRR303 pKa = 11.84 PDD305 pKa = 3.7 TDD307 pKa = 3.35 LTNWYY312 pKa = 9.43 GLALAGVQSYY322 pKa = 10.98 GSDD325 pKa = 3.47 YY326 pKa = 10.38 LTQTATIARR335 pKa = 11.84 ATSTANTSGMVDD347 pKa = 3.1 TGDD350 pKa = 3.35 VSLTKK355 pKa = 10.74 VMEE358 pKa = 4.68 GASS361 pKa = 3.42
Molecular weight: 38.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.505
IPC2_protein 4.622
IPC_protein 4.584
Toseland 4.38
ProMoST 4.749
Dawson 4.584
Bjellqvist 4.736
Wikipedia 4.533
Rodwell 4.418
Grimsley 4.291
Solomon 4.584
Lehninger 4.533
Nozaki 4.685
DTASelect 4.978
Thurlkill 4.418
EMBOSS 4.533
Sillero 4.711
Patrickios 3.427
IPC_peptide 4.571
IPC2_peptide 4.698
IPC2.peptide.svr19 4.66
Protein with the highest isoelectric point:
>tr|D2WKE3|D2WKE3_9VIRU Putative replicase protein OS=Chimpanzee associated porprismacovirus 1 OX=2170109 GN=rep PE=4 SV=1
MM1 pKa = 7.3 GHH3 pKa = 5.99 MFGYY7 pKa = 8.5 TCLKK11 pKa = 9.54 RR12 pKa = 11.84 GCPRR16 pKa = 11.84 RR17 pKa = 11.84 PLDD20 pKa = 3.25 YY21 pKa = 11.08 ALVYY25 pKa = 10.52 GVGISGHH32 pKa = 5.9 NDD34 pKa = 2.9 NDD36 pKa = 3.65 GTHH39 pKa = 5.19 PHH41 pKa = 6.02 EE42 pKa = 4.72 RR43 pKa = 11.84 RR44 pKa = 11.84 ANPLVQNLPRR54 pKa = 11.84 SRR56 pKa = 11.84 YY57 pKa = 8.26 SQMGYY62 pKa = 9.79 RR63 pKa = 11.84 AGGRR67 pKa = 11.84 SRR69 pKa = 11.84 RR70 pKa = 11.84 VQALAGQMQCAGCEE84 pKa = 3.8 RR85 pKa = 11.84 PAVDD89 pKa = 3.38 GLSTVRR95 pKa = 11.84 IRR97 pKa = 11.84 MGRR100 pKa = 11.84 GYY102 pKa = 10.44 LDD104 pKa = 3.16 SYY106 pKa = 10.03 RR107 pKa = 11.84 RR108 pKa = 11.84 SVRR111 pKa = 11.84 ISTSTRR117 pKa = 11.84 RR118 pKa = 11.84 KK119 pKa = 9.34 RR120 pKa = 11.84 ATIGHH125 pKa = 6.55 RR126 pKa = 11.84 GIRR129 pKa = 11.84 WAHH132 pKa = 5.33 VNSEE136 pKa = 4.13 FGKK139 pKa = 9.44 MRR141 pKa = 11.84 WNQEE145 pKa = 3.43 GALEE149 pKa = 4.1 ALQRR153 pKa = 11.84 TNDD156 pKa = 3.41 RR157 pKa = 11.84 EE158 pKa = 4.08 IVVWYY163 pKa = 9.81 DD164 pKa = 2.92 QDD166 pKa = 4.05 GNMGKK171 pKa = 9.23 SWLCGHH177 pKa = 7.6 LYY179 pKa = 8.32 EE180 pKa = 5.0 TGKK183 pKa = 10.73 AYY185 pKa = 10.16 YY186 pKa = 8.97 IPPYY190 pKa = 10.09 ISTIQSMIQTVASLVLQDD208 pKa = 4.91 RR209 pKa = 11.84 EE210 pKa = 3.99 AGYY213 pKa = 9.36 PPRR216 pKa = 11.84 PLIVIDD222 pKa = 5.23 IPRR225 pKa = 11.84 SWKK228 pKa = 9.63 WSTEE232 pKa = 3.64 LYY234 pKa = 9.64 TAIEE238 pKa = 4.54 AIKK241 pKa = 10.41 DD242 pKa = 3.74 GLIMDD247 pKa = 4.67 PRR249 pKa = 11.84 YY250 pKa = 9.71 GARR253 pKa = 11.84 PVNIHH258 pKa = 4.49 GTKK261 pKa = 10.52 VIVLTNTKK269 pKa = 9.98 PKK271 pKa = 10.2 LDD273 pKa = 3.97 KK274 pKa = 10.88 LSEE277 pKa = 4.3 DD278 pKa = 2.82 RR279 pKa = 11.84 WVLYY283 pKa = 10.74 DD284 pKa = 3.75 PLLYY288 pKa = 9.93 MM289 pKa = 5.73
Molecular weight: 33.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.8
IPC2_protein 9.428
IPC_protein 9.823
Toseland 9.692
ProMoST 9.648
Dawson 10.043
Bjellqvist 9.838
Wikipedia 10.292
Rodwell 10.145
Grimsley 10.16
Solomon 10.072
Lehninger 10.014
Nozaki 9.794
DTASelect 9.809
Thurlkill 9.853
EMBOSS 10.145
Sillero 9.97
Patrickios 4.838
IPC_peptide 10.058
IPC2_peptide 8.873
IPC2.peptide.svr19 8.252
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
650
289
361
325.0
35.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.385 ± 0.99
1.538 ± 0.126
6.462 ± 0.838
2.769 ± 0.683
2.615 ± 1.267
9.231 ± 0.074
1.538 ± 0.81
4.615 ± 1.063
3.692 ± 0.075
7.846 ± 0.074
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
4.154 ± 0.457
4.769 ± 0.863
5.385 ± 0.1
3.538 ± 0.176
6.769 ± 2.836
7.077 ± 0.787
7.077 ± 1.243
6.769 ± 0.584
1.692 ± 0.481
5.077 ± 0.531
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here