Candida viswanathii
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10576 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A367YFG1|A0A367YFG1_9ASCO Protein BIM1 OS=Candida viswanathii OX=5486 GN=BIM1_1 PE=3 SV=1
MM1 pKa = 8.11 DD2 pKa = 5.97 ANLEE6 pKa = 4.05 NTKK9 pKa = 8.51 MWEE12 pKa = 4.35 SVDD15 pKa = 4.21 HH16 pKa = 6.71 SLTSNLLSRR25 pKa = 11.84 ITSTVEE31 pKa = 3.46 CTICSEE37 pKa = 3.86 IMAVPVTAEE46 pKa = 4.35 CGHH49 pKa = 5.91 SFCYY53 pKa = 10.36 DD54 pKa = 4.35 CIYY57 pKa = 10.84 QWFSNKK63 pKa = 8.75 VNCPTCRR70 pKa = 11.84 HH71 pKa = 5.95 EE72 pKa = 5.0 IEE74 pKa = 4.73 NKK76 pKa = 7.93 PTLNLQLKK84 pKa = 7.16 EE85 pKa = 3.8 ICKK88 pKa = 10.25 NLLDD92 pKa = 4.43 MIIDD96 pKa = 3.67 SRR98 pKa = 11.84 LEE100 pKa = 3.64 NHH102 pKa = 6.46 EE103 pKa = 3.84 VLKK106 pKa = 10.49 NHH108 pKa = 6.66 KK109 pKa = 10.24 LEE111 pKa = 4.6 SEE113 pKa = 4.03 NNYY116 pKa = 10.32 NLDD119 pKa = 3.33 VRR121 pKa = 11.84 NKK123 pKa = 10.71 SLFGDD128 pKa = 4.45 LFNSTVTLIDD138 pKa = 3.58 NSDD141 pKa = 3.35 GVPRR145 pKa = 11.84 CGNCHH150 pKa = 5.93 WEE152 pKa = 3.82 AHH154 pKa = 6.1 GPVCQHH160 pKa = 6.39 CGTRR164 pKa = 11.84 FRR166 pKa = 11.84 VNPHH170 pKa = 5.02 SRR172 pKa = 11.84 FGGGYY177 pKa = 10.33 DD178 pKa = 4.7 DD179 pKa = 6.67 DD180 pKa = 6.58 DD181 pKa = 7.03 DD182 pKa = 4.9 EE183 pKa = 7.0 DD184 pKa = 4.23 EE185 pKa = 5.58 EE186 pKa = 5.02 SFQDD190 pKa = 4.35 VIAGYY195 pKa = 9.95 HH196 pKa = 6.18 RR197 pKa = 11.84 EE198 pKa = 3.85 LDD200 pKa = 3.77 TYY202 pKa = 11.3 DD203 pKa = 4.37 SEE205 pKa = 6.08 DD206 pKa = 3.47 SFIDD210 pKa = 3.14 SRR212 pKa = 11.84 TAHH215 pKa = 7.29 EE216 pKa = 4.86 IPVDD220 pKa = 3.63 MSDD223 pKa = 3.88 EE224 pKa = 4.64 DD225 pKa = 4.91 DD226 pKa = 4.56 VDD228 pKa = 5.24 DD229 pKa = 5.35 EE230 pKa = 4.99 GDD232 pKa = 3.57 NEE234 pKa = 4.94 RR235 pKa = 11.84 GDD237 pKa = 4.6 DD238 pKa = 4.61 SDD240 pKa = 5.62 DD241 pKa = 4.21 FSDD244 pKa = 4.12 NYY246 pKa = 10.76 EE247 pKa = 4.13 GDD249 pKa = 3.73 SADD252 pKa = 3.46 GDD254 pKa = 4.26 RR255 pKa = 11.84 NNSLLSADD263 pKa = 4.29 DD264 pKa = 4.92 DD265 pKa = 4.09 YY266 pKa = 12.1 HH267 pKa = 7.37 EE268 pKa = 5.22 LVLNGHH274 pKa = 7.08 PADD277 pKa = 3.52 EE278 pKa = 4.17 WHH280 pKa = 6.54 GFEE283 pKa = 4.68 SASSSMIDD291 pKa = 2.82 RR292 pKa = 11.84 ALSNDD297 pKa = 2.84 SDD299 pKa = 4.4 YY300 pKa = 11.21 QRR302 pKa = 11.84 HH303 pKa = 5.09 IHH305 pKa = 7.5 DD306 pKa = 5.8 DD307 pKa = 3.53 NDD309 pKa = 4.29 DD310 pKa = 4.14 DD311 pKa = 6.13 DD312 pKa = 5.52 EE313 pKa = 4.62 EE314 pKa = 4.96 SHH316 pKa = 5.35 TQEE319 pKa = 5.27 IEE321 pKa = 3.75 DD322 pKa = 3.89 DD323 pKa = 3.75 HH324 pKa = 7.47 PEE326 pKa = 4.24 RR327 pKa = 11.84 YY328 pKa = 9.38 HH329 pKa = 6.91 LSSPSSEE336 pKa = 4.64 NIIQLSGNDD345 pKa = 3.45 DD346 pKa = 3.45 DD347 pKa = 5.98 SYY349 pKa = 11.8 YY350 pKa = 11.01 DD351 pKa = 3.61 SEE353 pKa = 4.64 DD354 pKa = 3.25 VRR356 pKa = 11.84 EE357 pKa = 4.52 ALDD360 pKa = 4.73 DD361 pKa = 4.44 LDD363 pKa = 4.28 NVEE366 pKa = 5.65 ALLSYY371 pKa = 11.28 DD372 pKa = 3.79 NLIEE376 pKa = 4.83 DD377 pKa = 3.83 VSDD380 pKa = 4.94 DD381 pKa = 3.92 GLEE384 pKa = 4.3 DD385 pKa = 5.04 GYY387 pKa = 11.39 DD388 pKa = 3.5 SSGMVHH394 pKa = 7.1 ALEE397 pKa = 5.69 DD398 pKa = 5.28 DD399 pKa = 3.8 INLSDD404 pKa = 5.32 DD405 pKa = 3.71 YY406 pKa = 12.06
Molecular weight: 46.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.704
IPC2_protein 3.859
IPC_protein 3.897
Toseland 3.668
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.821
Rodwell 3.719
Grimsley 3.579
Solomon 3.884
Lehninger 3.846
Nozaki 3.986
DTASelect 4.253
Thurlkill 3.719
EMBOSS 3.821
Sillero 4.012
Patrickios 1.316
IPC_peptide 3.884
IPC2_peptide 3.999
IPC2.peptide.svr19 3.905
Protein with the highest isoelectric point:
>tr|A0A367XLL5|A0A367XLL5_9ASCO Vacuolar protein sorting-associated protein 33 OS=Candida viswanathii OX=5486 GN=VPS33_1 PE=3 SV=1
MM1 pKa = 7.74 TIRR4 pKa = 11.84 EE5 pKa = 4.2 KK6 pKa = 10.8 RR7 pKa = 11.84 SNCSVFVPSLEE18 pKa = 4.56 TKK20 pKa = 10.43 CLLYY24 pKa = 9.78 LTQSLCNPGGKK35 pKa = 8.89 IGLFNVFANHH45 pKa = 6.56 RR46 pKa = 11.84 SMARR50 pKa = 11.84 SNSLFGKK57 pKa = 9.11 VRR59 pKa = 11.84 WRR61 pKa = 11.84 NGG63 pKa = 2.83
Molecular weight: 7.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.34
IPC2_protein 9.633
IPC_protein 10.218
Toseland 10.789
ProMoST 10.394
Dawson 10.847
Bjellqvist 10.54
Wikipedia 11.023
Rodwell 11.155
Grimsley 10.877
Solomon 10.979
Lehninger 10.95
Nozaki 10.789
DTASelect 10.526
Thurlkill 10.76
EMBOSS 11.169
Sillero 10.789
Patrickios 10.95
IPC_peptide 10.979
IPC2_peptide 9.838
IPC2.peptide.svr19 8.54
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10576
0
10576
5109780
25
4929
483.1
54.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.977 ± 0.026
1.071 ± 0.008
6.057 ± 0.022
6.768 ± 0.033
4.386 ± 0.018
5.434 ± 0.028
2.145 ± 0.012
6.274 ± 0.022
6.821 ± 0.031
9.508 ± 0.033
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.848 ± 0.01
5.449 ± 0.022
4.892 ± 0.028
4.179 ± 0.026
4.174 ± 0.018
8.297 ± 0.037
6.081 ± 0.042
6.097 ± 0.025
1.016 ± 0.008
3.527 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here